Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,076,988	Δ1 bp	100%	coding (2195/3051 nt)	fdoG ←	formate dehydrogenase‑O, large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,076,988	0	G	.	100.0%	347.5 / NA	72	coding (2195/3051 nt)	fdoG	formate dehydrogenase‑O, large subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (37/35);  total (37/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCG  >  CP009273/4076844‑4077129                                                                                                                                                 |                                                                                                                                              taCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCg                                                                                                                                          <  2:702006/149‑1 (MQ=255)  aCAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGGGTTACCGAGGCCAGACGGATCGGCGTTATCACCGCGTGCCATCTGGTTGCCTTCCGGCGTCCCGCTACCGGCGAAAAACCAGCAGCCCCAGGAGGGT‑TACCGTc                                                                                                                                        >  2:622912/1‑150 (MQ=255)   cAGAATGCGGCGGTTAAGCGGCCATGCCCCTGGCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGGCCAGCCACCGGCGCAAATCCAGCCGCCAAAGGAGGTT‑TACCGTct                                                                                                                                       >  2:231703/1‑149 (MQ=255)    aGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCAt                                                                                                                                      <  1:978040/150‑1 (MQ=255)     gAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATc                                                                                                                                     <  1:47325/150‑1 (MQ=255)        tGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATcgcg                                                                                                                                  <  1:1006209/150‑1 (MQ=255)              ggTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTg                                                                                                                            >  1:315273/1‑150 (MQ=255)                 tAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGc                                                                                                                         >  1:214698/1‑150 (MQ=255)                     cGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGcgaac                                                                                                                     >  2:259327/1‑150 (MQ=255)                        ccatgcccatgcCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGcgaacgaa                                                                                                                  <  1:1191747/150‑1 (MQ=255)                        ccatgcccatgcCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGcgaacgaa                                                                                                                  >  1:32717/1‑150 (MQ=255)                         catgcccatgccCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCgaacgaac                                                                                                                 >  1:909290/1‑150 (MQ=255)                          atgcccatgcccAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCgaacgaac                                                                                                                 >  1:851783/1‑149 (MQ=255)                          atgcccatgcccAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACt                                                                                                                <  1:863072/150‑1 (MQ=255)                              ccatgcccaGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAgt                                                                                                            <  1:205415/150‑1 (MQ=255)                                    ccagcccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTgg                                                                                                      >  2:320358/1‑150 (MQ=255)                                     cagcccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGc                                                                                                     <  2:657836/150‑1 (MQ=255)                                     cagcccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGc                                                                                                     >  1:250393/1‑150 (MQ=255)                                      agcccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGcc                                                                                                    >  2:471867/1‑150 (MQ=255)                                      agcccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGcc                                                                                                    >  1:929304/1‑150 (MQ=255)                                         ccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCttt                                                                                                 >  1:426239/1‑150 (MQ=255)                                         ccagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCttt                                                                                                 >  2:147025/1‑150 (MQ=255)                                          cagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTc                                                                                                >  2:545579/1‑150 (MQ=255)                                          cagcGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTc                                                                                                >  2:860296/1‑150 (MQ=255)                                                      gAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAAcc                                                                                    <  1:897538/150‑1 (MQ=255)                                                       aGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCg                                                                                   <  1:147025/150‑1 (MQ=255)                                                       aGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCg                                                                                   >  2:197936/1‑150 (MQ=255)                                                          ccAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCAc                                                                                >  1:52063/1‑150 (MQ=255)                                                          ccAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCAc                                                                                <  2:32717/150‑1 (MQ=255)                                                           cAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCAcc                                                                               <  1:679940/150‑1 (MQ=255)                                                           cAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCAcc                                                                               >  2:631607/1‑150 (MQ=255)                                                            aGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCg                                                                              <  2:681703/150‑1 (MQ=255)                                                             gACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCCTCGCGCCATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCgg                                                                             >  2:751277/1‑150 (MQ=255)                                                              aCGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGt                                                                            <  1:230381/150‑1 (MQ=255)                                                                ggATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTg                                                                          >  2:61570/1‑150 (MQ=255)                                                                  aTCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGcc                                                                        >  2:71325/1‑150 (MQ=255)                                                                      gCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGAGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGAt                                                                    <  2:879637/150‑1 (MQ=255)                                                                       cGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATc                                                                   <  2:315273/150‑1 (MQ=255)                                                                           aTCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGt                                                                <  1:622120/149‑1 (MQ=255)                                                                            tCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTaa                                                              >  1:608371/1‑150 (MQ=255)                                                                                ggCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATc                                                          <  1:980822/150‑1 (MQ=255)                                                                                   gTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGc                                                       >  1:139512/1‑150 (MQ=255)                                                                                   gTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGc                                                       >  1:270841/1‑150 (MQ=255)                                                                                    tGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGcc                                                      <  2:214698/150‑1 (MQ=255)                                                                                    tGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGc                                                       >  1:647494/1‑149 (MQ=255)                                                                                       cATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAgc                                                   >  1:524900/1‑150 (MQ=255)                                                                                        aTCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAgcg                                                  >  2:985463/1‑150 (MQ=255)                                                                                          cTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGcc                                                >  1:72744/1‑150 (MQ=255)                                                                                             gTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCt                                               <  1:65024/148‑1 (MQ=255)                                                                                                gCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTAcc                                           <  1:180491/149‑1 (MQ=255)                                                                                                 ccTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCg                                          <  2:13390/149‑1 (MQ=255)                                                                                                 ccTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGt                                         <  1:259327/150‑1 (MQ=255)                                                                                                     ccGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCt                                               >  2:1012705/1‑140 (MQ=255)                                                                                                      cGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCtt                                              <  1:1012705/140‑1 (MQ=255)                                                                                                      cGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGctc                                    >  2:774/1‑150 (MQ=255)                                                                                                       ggCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGctct                                   >  2:827306/1‑150 (MQ=255)                                                                                                               gCTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGc                            >  1:890545/1‑149 (MQ=255)                                                                                                                cTACCGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCa                          <  1:471867/150‑1 (MQ=255)                                                                                                                   ccGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCActt                       <  2:909290/150‑1 (MQ=255)                                                                                                                    cGGCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCActtc                      <  1:798936/150‑1 (MQ=255)                                                                                                                      gCGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCActtctt                    >  2:866731/1‑150 (MQ=255)                                                                                                                       cGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACttcttc                   <  2:496842/150‑1 (MQ=255)                                                                                                                       cGAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACttcttc                   <  2:159334/150‑1 (MQ=255)                                                                                                                        gAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTtcttct                  >  2:676463/1‑150 (MQ=255)                                                                                                                        gAAAATCCAGCAGCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTtcttct                  <  1:71325/150‑1 (MQ=255)                                                                                                                               cagcagCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGAt            <  2:851783/149‑1 (MQ=255)                                                                                                                               cagcagCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGAt            <  2:139512/149‑1 (MQ=255)                                                                                                                               cagcagCCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATg           <  2:79902/150‑1 (MQ=255)                                                                                                                                    gcCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCa      >  1:347105/1‑150 (MQ=255)                                                                                                                                    gcCACAGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCa      <  2:433792/150‑1 (MQ=255)                                                                                                                                        caGGAGGTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCg  <  1:631607/150‑1 (MQ=255)                                                                                                                                         aggaggTT‑TACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCg  >  1:814493/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                              TACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTGTTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTACCGGCGAAAATCCAGCAGCCACAGGAGGTTGTACCGTCATCGCGCAATTGGGCGAACGAACTAAGTTGTTGGCCTTTCTTGACGATAACCGCACCGGTTGCCGGATCGGTAATATCGGCCAGCGCCTTACCGTTGCTCTCCATCGCCACTTCTTCTGAAGATGGCTCATGCG  >  CP009273/4076844‑4077129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑