Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	684,503:1	+A	22.2%	intergenic (‑34/+296)	insH1 ← / ← lnt	IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	684,503	1	.	A	22.2%	160.7 / 79.7	72	intergenic (‑34/+296)	insH1/lnt	IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base . (29/27);  new base A (7/9);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 7.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTA  >  CP009273/684360‑684649                                                                                                                                                 |                                                                                                                                                   aaGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTaa                                                                                                                                              >  1:259477/1‑150 (MQ=31)   gAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACa                                                                                                                                            >  1:124587/1‑150 (MQ=32)    aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACAt                                                                                                                                           <  2:767134/150‑1 (MQ=32)      aaTCTCTTTTCTGGTCTGACGGCGCTTATTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACAtt                                                                                                                                          >  1:763416/1‑149 (MQ=18)       aTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTa                                                                                                                                        <  2:112387/150‑1 (MQ=35)         ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGt                                                                                                                                      <  1:403041/150‑1 (MQ=37)              ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGttt                                                                                                                                    <  1:909067/147‑1 (MQ=38)              ttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTg                                                                                                                                   <  2:521708/148‑1 (MQ=39)                cTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGc                                                                                                                                 >  2:1066172/1‑149 (MQ=255)                 tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTa                                                                                                                               >  2:740636/1‑149 (MQ=255)                    tCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATg                                                                                                                           <  2:470730/150‑1 (MQ=255)                        aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTaa                                                                                                                        <  2:609134/150‑1 (MQ=255)                        aCGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGt                                                                                                                          <  1:639488/147‑1 (MQ=255)                        aCGGCGATTAATGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACGCATGATGAACCCTGTTCTATGGCTACAGATGACAAACATGATCTCATATCAGGGAGTTGTTCGTGCCTTCCAT‑AGGTAAGATTTAGTTTGGCGAAATGt                                                                                                                          <  2:515491/147‑1 (MQ=14)                         cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTa                                                                                                                         >  2:16050/1‑147 (MQ=255)                          ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTa                                                                                                                         >  2:444026/1‑146 (MQ=255)                          ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGa                                                                                                                     <  2:259477/150‑1 (MQ=255)                           gcgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGa                                                                                                                     <  1:284281/150‑1 (MQ=255)                            cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGata                                                                                                                   <  1:92997/150‑1 (MQ=255)                            cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGata                                                                                                                   <  1:1005751/150‑1 (MQ=255)                                 aCTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATAtt                                                                                                                 <  2:299647/148‑1 (MQ=255)                                     ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGt                                                                                                            <  1:194828/148‑1 (MQ=255)                                         aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTAt                                                                                                       >  1:472897/1‑150 (MQ=255)                                            cACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTAttgc                                                                                                    <  1:740636/149‑2 (MQ=255)                                             aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTAttgt                                                                                                    <  2:240459/149‑1 (MQ=255)                                                   ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGttttt                                                                                            <  1:339003/150‑1 (MQ=255)                                                   ggCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGtttt                                                                                             <  2:47354/149‑1 (MQ=255)                                                        aGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGc                                                                                          >  1:265850/1‑147 (MQ=255)                                                         ggTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGc                                                                                          >  2:1161219/1‑147 (MQ=255)                                                         ggTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCgag                                                                                       <  2:265850/149‑1 (MQ=255)                                                           tAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATg                                                                                    >  2:1022438/1‑150 (MQ=255)                                                            aaGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATgc                                                                                   >  1:793206/1‑150 (MQ=255)                                                              gTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATgcgc                                                                                 >  1:827912/1‑150 (MQ=255)                                                               ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGcgcc                                                                                >  2:18500/1‑150 (MQ=255)                                                               ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGcgcc                                                                                >  2:132151/1‑150 (MQ=255)                                                                 gatgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGcgcc                                                                                <  1:386772/149‑1 (MQ=255)                                                                     aCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCAc                                                                            >  2:51784/1‑149 (MQ=255)                                                                      cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCa                                                                          >  2:842771/1‑150 (MQ=255)                                                                      cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAt                                                                         >  2:102002/1‑150 (MQ=255)                                                                      cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAt                                                                         <  2:694429/150‑1 (MQ=255)                                                                        cATGATGAACCCTGTTCTATGGCGCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTc                                                                       >  1:317339/1‑150 (MQ=255)                                                                               aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGc                                                                 >  1:594353/1‑149 (MQ=255)                                                                                aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCaa                                                               <  2:931809/150‑1 (MQ=255)                                                                                      ttCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCt                                                         >  2:604523/1‑150 (MQ=255)                                                                                          aTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTaa                                                      >  2:311424/1‑149 (MQ=255)                                                                                            ggCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAAt                                                   >  2:9939/1‑150 (MQ=255)                                                                                                 cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCaaaa                                                             >  1:349044/1‑135 (MQ=255)                                                                                                 cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCAct                                              >  2:1023727/1‑150 (MQ=255)                                                                                                 cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCAc                                               <  1:157335/149‑1 (MQ=255)                                                                                                  aGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAAt                                                            <  2:349044/135‑1 (MQ=255)                                                                                                     tgtcAAACATGAACTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCtt                                           <  2:348782/147‑1 (MQ=255)                                                                                                      gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCttt                                          >  2:528628/1‑149 (MQ=255)                                                                                                      gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCttt                                          >  2:435284/1‑149 (MQ=255)                                                                                                           aCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTg                                     <  1:314562/150‑1 (MQ=255)                                                                                                            cATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGc                                    >  1:835960/1‑149 (MQ=255)                                                                                                             aTGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCt                                   <  2:472897/150‑1 (MQ=255)                                                                                                                aTCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTAcc                                <  1:481258/149‑1 (MQ=255)                                                                                                                 tctcATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGc                              >  1:626900/1‑150 (MQ=255)                                                                                                                  ctcATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGc                              >  1:113480/1‑150 (MQ=255)                                                                                                                  ctcATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCt                             >  2:401698/1‑150 (MQ=255)                                                                                                                    cATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTg                            >  1:1169660/1‑149 (MQ=255)                                                                                                                     atatCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCt                             <  2:124587/147‑1 (MQ=255)                                                                                                                     atatCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTgg                           <  2:131353/149‑1 (MQ=255)                                                                                                                         cAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTAc                      >  1:621578/1‑150 (MQ=255)                                                                                                                          aGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTAc                      <  2:594353/149‑1 (MQ=255)                                                                                                                          aGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACt                     <  1:1023727/150‑1 (MQ=255)                                                                                                                          aGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACt                     >  1:712264/1‑150 (MQ=255)                                                                                                                            ggACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACtgt                   >  1:72713/1‑150 (MQ=255)                                                                                                                              aCTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACtgtg                  >  1:766052/1‑150 (MQ=255)                                                                                                                                     cGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAAc           <  1:102002/149‑1 (MQ=255)                                                                                                                                       cACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTa         <  1:1066172/150‑1 (MQ=255)                                                                                                                                       cACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAg        <  1:581874/150‑1 (MQ=255)                                                                                                                                           ttcctt‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTg    >  2:1090309/1‑150 (MQ=255)                                                                                                                                             cctt‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTg    <  2:906965/148‑1 (MQ=255)                                                                                                                                             cctt‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTa  >  1:472501/1‑150 (MQ=255)                                                                                                                                              ctt‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTa  <  1:16050/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   AAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTA  >  CP009273/684360‑684649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑