Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 9,092 C→T 54.9% H285H (CAC→CAT)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092739,0920CT54.9% 50.7 / 199.5 142H285H (CAC→CAT) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base C (31/33);  new base T (29/49);  total (60/82)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 145 total aligned reads displayed.

GCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCT  >  CP009273/8946‑9241
                                                                                                                                                  |                                                                                                                                                     
gCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGcaca                                                                                                                                                      <  2:1098841/148‑1 (MQ=255)
 cAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAAcc                                                                                                                                                   <  1:120382/150‑1 (MQ=255)
 cAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAAc                                                                                                                                                    >  1:460422/1‑149 (MQ=255)
  aGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCa                                                                                                                                                  <  1:293348/150‑1 (MQ=255)
   ggCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAg                                                                                                                                                 <  2:431098/150‑1 (MQ=255)
        tgACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGAt                                                                                                                                              <  1:753172/147‑1 (MQ=255)
         gACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATcc                                                                                                                                            >  1:552802/1‑149 (MQ=255)
             gccTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATgg                                                                                                                                       >  2:379293/3‑150 (MQ=255)
              tCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATgg                                                                                                                                       >  1:658935/1‑149 (MQ=255)
              ccTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGc                                                                                                                                      <  2:7601/149‑1 (MQ=255)
                 gACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGcgg                                                                                                                                    >  2:516100/1‑147 (MQ=255)
                  aCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAg                                                                                                                                    <  2:332904/148‑1 (MQ=255)
                  aCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTa                                                                                                                                  <  2:206543/150‑1 (MQ=255)
                   ccATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAg                                                                                                                                 <  2:930989/150‑1 (MQ=255)
                    cATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGa                                                                                                                                <  1:841011/150‑1 (MQ=255)
                      tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                                                                                              <  2:585747/150‑3 (MQ=255)
                      tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                                                                                              <  2:495315/150‑3 (MQ=255)
                      tCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATa                                                                                                                              <  2:300811/150‑1 (MQ=255)
                        gCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATaa                                                                                                                             <  2:555750/149‑1 (MQ=255)
                         ccccGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAAc                                                                                                                           >  1:351981/3‑150 (MQ=255)
                          cccGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACt                                                                                                                          <  1:702804/149‑1 (MQ=255)
                          aCCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACt                                                                                                                          >  2:69323/1‑150 (MQ=255)
                                       aaaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTAt                                                                                                              <  2:440491/149‑1 (MQ=255)
                                        aaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATcc                                                                                                            <  2:565520/150‑1 (MQ=255)
                                         agagCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCg                                                                                                           >  1:1073330/1‑150 (MQ=255)
                                         agagCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCg                                                                                                           <  1:571235/150‑1 (MQ=255)
                                         agagCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCg                                                                                                           <  2:500970/150‑1 (MQ=255)
                                          gagCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGt                                                                                                          <  2:617690/150‑1 (MQ=255)
                                           agCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTa                                                                                                         <  2:822847/150‑1 (MQ=255)
                                           agCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGt                                                                                                          >  1:771723/1‑149 (MQ=255)
                                            gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                                                                                        <  2:89771/150‑1 (MQ=255)
                                               gtccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                                                     >  1:305181/8‑150 (MQ=255)
                                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                                                                                    <  2:1095317/144‑1 (MQ=255)
                                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                                                                                    <  2:604448/144‑1 (MQ=255)
                                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                                                     <  1:292146/143‑1 (MQ=255)
                                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                                                     <  1:122294/143‑1 (MQ=255)
                                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                                                     <  1:192822/143‑1 (MQ=255)
                                                   gagaGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCt                                                                                                 <  1:677555/150‑1 (MQ=255)
                                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                              <  1:516100/150‑2 (MQ=255)
                                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                              >  1:201946/1‑149 (MQ=255)
                                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                              <  2:680495/150‑2 (MQ=255)
                                                       gCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtcg                                                                                             <  2:1040356/150‑3 (MQ=255)
                                                       gCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                              >  1:173339/1‑148 (MQ=255)
                                                        cGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGa                                                                                            >  2:24321/1‑150 (MQ=255)
                                                         gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                                                                                           >  2:772826/1‑150 (MQ=255)
                                                         gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                                                                                           >  2:412422/1‑150 (MQ=255)
                                                               gCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGa                                                                                      >  1:1042522/1‑149 (MQ=255)
                                                                cTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                                                    <  2:1060469/150‑1 (MQ=255)
                                                                 tATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                                                    >  1:391232/1‑149 (MQ=255)
                                                                 tATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaa                                                                                    >  2:250373/1‑149 (MQ=255)
                                                                  aTCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGaaaaa                                                                                  >  1:758653/1‑150 (MQ=255)
                                                                   tCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAc                                                                                 <  2:1128292/150‑1 (MQ=255)
                                                                   tCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAAc                                                                                 <  1:696100/150‑1 (MQ=255)
                                                                     gAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTg                                                                               >  1:980262/1‑150 (MQ=255)
                                                                       aCGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTgg                                                                              >  1:624261/1‑149 (MQ=255)
                                                                            aaCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaa                                                                         <  1:1134194/149‑1 (MQ=255)
                                                                               tGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGa                                                                     >  1:168140/1‑150 (MQ=255)
                                                                                 gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGa                                                                     <  2:748836/142‑1 (MQ=255)
                                                                                 gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                                                   >  2:536646/7‑150 (MQ=255)
                                                                                 gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                                                   >  2:645393/7‑150 (MQ=255)
                                                                                 gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                                                   >  2:1145094/7‑150 (MQ=255)
                                                                                 gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                                                   <  2:1119199/144‑1 (MQ=255)
                                                                                    tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                                                <  1:977653/147‑3 (MQ=255)
                                                                                    tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                                                >  1:1025510/4‑148 (MQ=255)
                                                                                    tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCga                                                                 <  2:457801/146‑2 (MQ=255)
                                                                                    tACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGc                                                                >  2:802581/1‑150 (MQ=255)
                                                                                      tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                               <  1:384967/148‑1 (MQ=255)
                                                                                      cacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCg                                                               <  1:1064288/149‑1 (MQ=255)
                                                                                        ccGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATc                                                            >  2:522803/1‑150 (MQ=255)
                                                                                         cGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAct                                                           >  2:1024700/1‑150 (MQ=255)
                                                                                         cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                           <  1:407673/150‑1 (MQ=255)
                                                                                          ggtgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActg                                                          <  2:723468/147‑1 (MQ=255)
                                                                                          ggtgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActg                                                          >  2:326476/4‑150 (MQ=255)
                                                                                            tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                                        <  1:946813/149‑1 (MQ=255)
                                                                                            tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                                        <  1:668224/149‑1 (MQ=255)
                                                                                             gAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgctg                                                       >  2:1013747/1‑150 (MQ=255)
                                                                                             gAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAt                                                                                               >  1:670427/1‑110 (MQ=255)
                                                                                             gAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                                                       <  2:864323/150‑1 (MQ=255)
                                                                                              aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                              <  2:670427/110‑2 (MQ=255)
                                                                                              aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                                                       >  2:408503/1‑149 (MQ=255)
                                                                                              aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                      <  2:1153484/150‑1 (MQ=255)
                                                                                               aGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTa                                                     <  1:642624/150‑1 (MQ=255)
                                                                                                gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaa                                                             >  1:1169086/1‑140 (MQ=255)
                                                                                                gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                                                    <  1:1057865/150‑1 (MQ=255)
                                                                                                 tGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                                                   <  2:95272/150‑1 (MQ=255)
                                                                                                 tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                      >  1:737485/1‑147 (MQ=255)
                                                                                                 tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                      >  1:1120585/1‑147 (MQ=255)
                                                                                                  gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAATAAATGATCGACGAACTGCTGTaa                                                    >  1:661029/1‑148 (MQ=255)
                                                                                                   aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag                                                 <  2:852528/150‑4 (MQ=255)
                                                                                                   aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaaga                                                  <  1:849142/149‑3 (MQ=255)
                                                                                                     aGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa                                               >  2:1190756/1‑145 (MQ=255)
                                                                                                             cGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGt                                       <  2:461997/150‑1 (MQ=255)
                                                                                                                        aCTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGt                            >  1:841643/1‑150 (MQ=255)
                                                                                                                          tGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCg                          >  2:356791/1‑150 (MQ=255)
                                                                                                                               ccGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCAc                     >  1:90232/1‑150 (MQ=255)
                                                                                                                                          tgGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACctct            <  1:424514/148‑1 (MQ=255)
                                                                                                                                          tgGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCtcttc          >  2:723171/1‑150 (MQ=255)
                                                                                                                                          tgGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCtctt           <  1:356791/149‑1 (MQ=255)
                                                                                                                                              agcaCAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTg        >  2:905699/1‑148 (MQ=255)
                                                                                                                                                  caACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCct  <  2:1042522/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCT  >  CP009273/8946‑9241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑