Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 9,178 G→A 25.5% G314D (GGC→GAC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092739,1780GA25.5% 161.5 / 68.3 94G314D (GGC→GAC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base G (35/35);  new base A (11/13);  total (46/48)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

TACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGC  >  CP009273/9030‑9326
                                                                                                                                                    |                                                                                                                                                    
tACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGc                                                                                                                                                     >  2:802581/1‑150 (MQ=255)
 acacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGc                                                                                                                                                     <  1:900858/149‑1 (MQ=255)
  tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                                                                                    <  1:727675/148‑1 (MQ=255)
  tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                                                                                    <  1:384967/148‑1 (MQ=255)
  cacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCg                                                                                                                                                    <  1:1064288/149‑1 (MQ=255)
    ccGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATGCGTAAGTTTGCGATCGGCCAGGAAAAACTGGAAAAAATGATcgacgaac                                                                                                                                                 <  2:1025558/150‑3 (MQ=255)
    ccGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATc                                                                                                                                                 >  2:522803/1‑150 (MQ=255)
     cGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAct                                                                                                                                                >  2:1024700/1‑150 (MQ=255)
     cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGGGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                                                                                                                >  2:424514/1‑150 (MQ=255)
     cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                                                                                                                <  2:230251/150‑1 (MQ=255)
     cGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATct                                                                                                                                                <  1:407673/150‑1 (MQ=255)
      ggtgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActg                                                                                                                                               >  2:326476/4‑150 (MQ=255)
      ggtgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActg                                                                                                                                               <  2:723468/147‑1 (MQ=255)
      ggCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctg                                                                                                                                               <  2:796720/150‑1 (MQ=255)
        tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                                                                                                                             <  1:668224/149‑1 (MQ=255)
        tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                                                                                                                             <  1:946813/149‑1 (MQ=255)
         gAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATctgctg                                                                                                                                            >  2:1013747/1‑150 (MQ=255)
         gAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                                                                                                                                            <  2:864323/150‑1 (MQ=255)
          aaGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGt                                                                                                                                           <  1:219786/150‑1 (MQ=255)
          aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                                                                                                                                            >  2:408503/1‑149 (MQ=255)
          aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgctg                                                                                                                                            >  1:598130/1‑149 (MQ=255)
          aaGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                                                                                           <  2:1153484/150‑1 (MQ=255)
           aGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTa                                                                                                                                          <  2:635552/150‑1 (MQ=255)
           aGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTa                                                                                                                                          <  1:642624/150‑1 (MQ=255)
            gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaa                                                                                                                                                  >  1:1169086/1‑140 (MQ=255)
            gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                                                                                                                                         <  1:1057865/150‑1 (MQ=255)
             tGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                                                                                                                                        <  2:95272/150‑1 (MQ=255)
             tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaac                                                                                                                                                 <  2:1169086/141‑3 (MQ=255)
             tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                                                                                           >  1:737485/1‑147 (MQ=255)
             tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                                                                                           >  1:1120585/1‑147 (MQ=255)
              gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAATAAATGATCGACGAACTGCTGTaa                                                                                                                                         >  1:661029/1‑148 (MQ=255)
               aaaGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATc                                                                                                                                       <  2:460422/149‑1 (MQ=255)
               aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag                                                                                                                                      <  2:852528/150‑4 (MQ=255)
               aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaaga                                                                                                                                       <  1:849142/149‑3 (MQ=255)
                aaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagaga                                                                                                                                     <  1:972620/150‑5 (MQ=255)
                aaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagag                                                                                                                                      >  2:572042/1‑146 (MQ=255)
                 aGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa                                                                                                                                    >  2:1190756/1‑145 (MQ=255)
                  gcgcGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa                                                                                                                                    >  2:404614/1‑144 (MQ=255)
                   cgcgTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa                                                                                                                                    >  1:995845/1‑143 (MQ=255)
                         cGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGt                                                                                                                            <  2:461997/150‑1 (MQ=255)
                                    aCTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAg                                                                                                                  <  2:695746/149‑1 (MQ=255)
                                    aCTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGt                                                                                                                 >  1:841643/1‑150 (MQ=255)
                                      tGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCg                                                                                                               >  2:356791/1‑150 (MQ=255)
                                           ccGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCAc                                                                                                          >  1:90232/1‑150 (MQ=255)
                                             gAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGc                                                                                                        >  1:535888/1‑150 (MQ=255)
                                                  ccTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTAcc                                                                                                    <  2:980262/149‑1 (MQ=255)
                                                      tgGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACctct                                                                                                 <  1:424514/148‑1 (MQ=255)
                                                      tgGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCtcttc                                                                                               >  2:723171/1‑150 (MQ=255)
                                                      tgGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCtctt                                                                                                <  1:356791/149‑1 (MQ=255)
                                                          agcaCAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTg                                                                                             >  2:905699/1‑148 (MQ=255)
                                                              caACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCct                                                                                       <  2:1042522/150‑1 (MQ=255)
                                                              caACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCct                                                                                       >  1:753667/1‑150 (MQ=255)
                                                               aaCCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCctg                                                                                      >  1:964611/1‑150 (MQ=255)
                                                               aaCCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCctg                                                                                      >  1:258702/1‑150 (MQ=255)
                                                                  cAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCctgtct                                                                                   >  2:849831/1‑150 (MQ=255)
                                                                  cAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCctgtct                                                                                   >  1:28607/1‑150 (MQ=255)
                                                                     gATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCctgtctgt                                                                                 >  2:532789/1‑149 (MQ=255)
                                                                         cAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACt                                                                             <  1:1161296/149‑1 (MQ=255)
                                                                          aaTGGCAGTAGATAAACTGGCGGAAGGTATACGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTAACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGACTGTCTGTCACAcc                                                                           <  2:951686/150‑1 (MQ=255)
                                                                           aTGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTccc                                                                          >  2:908432/1‑150 (MQ=255)
                                                                           aTGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTccc                                                                          >  2:942954/1‑150 (MQ=255)
                                                                           aTGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTcac                                                                          <  2:758653/150‑3 (MQ=255)
                                                                                    gATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAgtg                                                                 <  2:771723/150‑1 (MQ=255)
                                                                                    gATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAgtg                                                                 >  1:298568/1‑150 (MQ=255)
                                                                                    gATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAgtg                                                                 >  2:6457/1‑150 (MQ=255)
                                                                                    gATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAgtg                                                                 <  2:624261/150‑1 (MQ=255)
                                                                                    gATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAgtg                                                                 <  2:120707/150‑1 (MQ=255)
                                                                                    gATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAgtg                                                                 <  1:522803/150‑1 (MQ=255)
                                                                                      tAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTa                                                               >  1:190184/1‑150 (MQ=255)
                                                                                              cGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTg                                                       >  1:581962/1‑150 (MQ=255)
                                                                                                gAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGtt                                                     <  1:723171/150‑1 (MQ=255)
                                                                                                 aaGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGttt                                                    <  1:412422/150‑1 (MQ=255)
                                                                                                     tATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACg                                                <  2:391232/150‑1 (MQ=255)
                                                                                                     tATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACg                                                <  2:658935/150‑1 (MQ=255)
                                                                                                       tCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAAc                                                 >  2:1034310/1‑147 (MQ=255)
                                                                                                        ccGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACgaga                                             >  1:14836/1‑150 (MQ=255)
                                                                                                         cGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGAc                                            >  2:416509/1‑150 (MQ=255)
                                                                                                           tAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTg                                          >  1:665424/1‑150 (MQ=255)
                                                                                                           tAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTg                                          >  2:1067239/1‑150 (MQ=255)
                                                                                                                 tGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTaa                                     <  2:753667/149‑1 (MQ=255)
                                                                                                                   cTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAAc                                   <  1:676254/149‑1 (MQ=255)
                                                                                                                   cTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAAc                                   <  2:168140/149‑1 (MQ=255)
                                                                                                                          ccAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAAt                           <  2:46191/150‑1 (MQ=255)
                                                                                                                           cAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATc                          >  2:1037414/1‑150 (MQ=255)
                                                                                                                            aGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCt                         >  2:435609/1‑150 (MQ=255)
                                                                                                                            aGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCt                         >  2:755110/1‑150 (MQ=255)
                                                                                                                            aGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCt                         <  1:772826/150‑1 (MQ=255)
                                                                                                                             ggAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTc                                                         >  2:752513/1‑117 (MQ=255)
                                                                                                                             ggAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCtt                        >  1:236251/1‑150 (MQ=255)
                                                                                                                              gAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCt                                                        <  1:752513/117‑1 (MQ=255)
                                                                                                                                  aaCTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTtgatga                   <  1:849831/150‑1 (MQ=255)
                                                                                                                                    cTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAAt                 <  2:355758/150‑1 (MQ=255)
                                                                                                                                        aaaaaaTGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACtt             <  2:258702/150‑1 (MQ=255)
                                                                                                                                            aaTGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACg         >  1:363980/1‑150 (MQ=255)
                                                                                                                                              tGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTa       <  1:497110/150‑1 (MQ=255)
                                                                                                                                               gATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTAt      >  2:263609/1‑150 (MQ=255)
                                                                                                                                                  cGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTgg   >  1:45127/1‑150 (MQ=255)
                                                                                                                                                   ggCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTgg   <  2:298568/149‑1 (MQ=255)
                                                                                                                                                   ggCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGc  >  1:204824/1‑150 (MQ=255)
                                                                                                                                                   ggCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGc  <  1:905699/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGCAGTGTATCATTCTGTTTAACGAGACTGTTTAAACGGAAAAATCTTGATGAATACTTTACGTATTGGC  >  CP009273/9030‑9326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑