BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	8,324	G→T	35.7%	P29P (CCG→CCT)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	8,324	0	G	T	35.7%	140.1 / 141.9	112	P29P (CCG→CCT)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base G (35/37); new base T (22/18); total (57/55)
Fisher's exact test for biased strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.96e-06

Only 100 of 113 total aligned reads displayed.

TGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGG  >  CP009273/8176‑8469

tGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCg                                                                                                                                                   >  1:720596/1‑149 (MQ=255)

  atatCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAg                                                                                                                                                <  2:220589/150‑1 (MQ=255)

     tcatcaGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGAt                                                                                                                                             <  1:208494/150‑1 (MQ=255)

         caGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCca                                                                                                                                         >  2:155379/1‑150 (MQ=255)

             gCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaac                                                                                                                                     <  1:236257/150‑1 (MQ=255)

              cAGACCGGTTACATCCCTCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaac                                                                                                                                     >  1:134259/1‑149 (MQ=255)

              cAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaac                                                                                                                                     >  1:360873/1‑149 (MQ=255)

               aGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaacca                                                                                                                                   <  2:360873/150‑1 (MQ=255)

                       tACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTtct                                                                                                                           >  2:771332/1‑150 (MQ=255)

                          aTCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTtctctc                                                                                                                        <  2:433504/150‑1 (MQ=255)

                              cccTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTc                                                                                                                    <  2:768965/150‑1 (MQ=255)

                                cTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCtt                                                                                                                  <  2:851495/150‑1 (MQ=255)

                                   aCAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCTCAACCAACCCTTCTCTCATTCTTAAc                                                                                                               <  1:905634/150‑1 (MQ=255)

                                     aaGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgc                                                                                                             <  2:947455/150‑1 (MQ=255)

                                            ttAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcg                                                                                                         >  1:442265/1‑147 (MQ=255)

                                               aaGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGAt                                                                                                    <  1:658031/149‑1 (MQ=255)

                                                agagAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTc                                                                                                  >  2:653806/1‑150 (MQ=255)

                                                  agaAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGAGGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCg                                                                                                >  1:1042122/1‑150 (MQ=255)

                                                     aaTACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGaa                                                                                             <  2:833653/150‑1 (MQ=255)

                                                        aCTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATAc                                                                                          >  2:102263/1‑150 (MQ=255)

                                                        aCTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATAc                                                                                          >  1:1052834/1‑150 (MQ=255)

                                                         cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATAcc                                                                                         <  2:1028636/150‑1 (MQ=255)

                                                         cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATAcc                                                                                         <  1:750697/150‑1 (MQ=255)

                                                         acaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTAcc                                                                                         >  1:315741/6‑150 (MQ=255)

                                                         acaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTAcc                                                                                         >  1:428332/6‑150 (MQ=255)

                                                          caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                                                                                        <  2:266040/146‑1 (MQ=255)

                                                          caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                                                                                        <  1:468668/146‑1 (MQ=255)

                                                          caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                                                                                        <  2:369418/146‑1 (MQ=255)

                                                           aaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                                                                                       <  1:388126/147‑1 (MQ=255)

                                                            tCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCg                                                                                        <  2:483465/148‑1 (MQ=255)

                                                             cATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGt                                                                                       >  2:787570/1‑148 (MQ=255)

                                                             cATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGt                                                                                       >  1:678858/1‑148 (MQ=255)

                                                             aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                                                                                       >  2:257851/2‑148 (MQ=255)

                                                              aTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAg                                                                                    >  1:734995/1‑150 (MQ=255)

                                                              aTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAg                                                                                    <  2:1174033/150‑1 (MQ=255)

                                                                gACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGtt                                                                                  >  2:1126570/1‑150 (MQ=255)

                                                                  cGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctga                                                                                >  2:1038402/1‑150 (MQ=255)

                                                                  cGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttga                                                                                >  2:633300/1‑150 (MQ=255)

                                                                   ggATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgat                                                                               <  1:1075467/150‑1 (MQ=255)

                                                                   ggATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgat                                                                               <  2:49848/150‑1 (MQ=255)

                                                                    gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgatt                                                                              >  2:1192639/1‑150 (MQ=255)

                                                                     aCAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgatt                                                                              >  1:817462/1‑149 (MQ=255)

                                                                      taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga                                                                            <  2:531907/149‑1 (MQ=255)

                                                                      taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga                                                                            >  2:1169343/2‑150 (MQ=255)

                                                                      taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga                                                                            <  2:809958/149‑1 (MQ=255)

                                                                        aaTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTtgattgat                                                                           >  1:214340/1‑149 (MQ=255)

                                                                        aaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattgac                                                                           <  1:934558/149‑2 (MQ=255)

                                                                        aaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattgac                                                                           <  2:760692/149‑2 (MQ=255)

                                                                          ttGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTgatgat                                                                        <  2:632217/150‑1 (MQ=255)

                                                                             aCCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCt                                                                     <  2:868868/150‑1 (MQ=255)

                                                                                   cTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGcc                                                               <  2:421435/150‑1 (MQ=255)

                                                                                    ttCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCt                                                              >  2:1007092/1‑150 (MQ=255)

                                                                                     gcGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTg                                                             >  1:457934/2‑150 (MQ=255)

                                                                                      cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                                                            <  1:960182/150‑1 (MQ=255)

                                                                                       gTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGAGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTggg                                                           <  1:80216/150‑1 (MQ=255)

                                                                                       gTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTggg                                                           >  1:1191317/1‑150 (MQ=255)

                                                                                       gTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTggg                                                           >  1:564387/1‑150 (MQ=255)

                                                                                       gTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTgg                                                            <  2:134679/149‑1 (MQ=255)

                                                                                        tCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGc                                                          <  2:134259/150‑1 (MQ=255)

                                                                                         cAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGc                                                          >  2:409302/1‑149 (MQ=255)

                                                                                           gTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcg                                                       <  1:831030/150‑4 (MQ=255)

                                                                                           gTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcg                                                       <  2:30185/150‑4 (MQ=255)

                                                                                              caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag                                                    >  2:875033/1‑144 (MQ=255)

                                                                                              caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag                                                    <  1:816999/150‑7 (MQ=255)

                                                                                              caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag                                                    >  1:511431/1‑144 (MQ=255)

                                                                                              caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag                                                    >  2:896458/1‑144 (MQ=255)

                                                                                              caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag                                                    >  2:615151/1‑144 (MQ=255)

                                                                                              caccacCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa                                                      >  1:672247/1‑148 (MQ=255)

                                                                                              caccacCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAc                                                     <  1:633300/149‑1 (MQ=255)

                                                                                               accaccGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcag                                                   <  2:1052834/150‑1 (MQ=255)

                                                                                                ccaccGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagc                                                  <  2:785236/150‑1 (MQ=255)

                                                                                                   ccGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCaga                                               <  2:817462/150‑1 (MQ=255)

                                                                                                      tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCa                                            <  1:1026921/150‑1 (MQ=255)

                                                                                                      tAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCa                                            <  1:771332/150‑1 (MQ=255)

                                                                                                       aGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCaa                                           >  2:918938/1‑150 (MQ=255)

                                                                                                        gTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAAc                                          <  2:1075961/150‑1 (MQ=255)

                                                                                                        gTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAAc                                          >  1:528165/1‑150 (MQ=255)

                                                                                                              gACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgc                                    <  2:720596/150‑1 (MQ=255)

                                                                                                                 acTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATcgcgcg                                 >  1:1142939/1‑150 (MQ=255)

                                                                                                                     gggACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCgcagc                             <  1:725406/150‑1 (MQ=255)

                                                                                                                         tattgcgGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCgcag                              >  2:420077/5‑145 (MQ=255)

                                                                                                                         tattgcgGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCgcag                              >  1:253692/5‑145 (MQ=255)

                                                                                                                         tattgcgGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCgcag                              >  1:1132482/5‑145 (MQ=255)

                                                                                                                         cATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGAt                         <  1:779517/150‑1 (MQ=255)

                                                                                                                          attgcgGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATc                        >  2:955598/4‑150 (MQ=255)

                                                                                                                          aTCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATc                        <  1:918938/150‑1 (MQ=255)

                                                                                                                              cgGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATcctcg                    <  2:315741/150‑5 (MQ=255)

                                                                                                                               ggCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGa                   >  1:325155/1‑150 (MQ=255)

                                                                                                                                gCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAc                  <  1:787570/150‑1 (MQ=255)

                                                                                                                                 cAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATcctcgatg                 >  2:402266/1‑143 (MQ=255)

                                                                                                                                 cAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATcctcgatg                 >  1:839056/1‑143 (MQ=255)

                                                                                                                                 cAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcg                 >  1:157184/1‑150 (MQ=255)

                                                                                                                                    tGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCgca                               >  1:133896/1‑133 (MQ=255)

                                                                                                                                     gAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCgcag                              <  2:133896/133‑1 (MQ=255)

                                                                                                                                     gAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGcgac             >  2:17371/1‑150 (MQ=255)

                                                                                                                                      aaGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGcgacc            <  2:5709/150‑1 (MQ=255)

                                                                                                                                      aaGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGcgacc            <  1:1122061/150‑1 (MQ=255)

                                                                                                                                            tATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACaaa      >  2:425519/1‑150 (MQ=255)

                                                                                                                                                aaCCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTg   <  1:1034559/149‑1 (MQ=255)

                                                                                                                                                  ccGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTgg  >  2:363146/1‑148 (MQ=255)

TGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGG  >  CP009273/8176‑8469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`