Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,555,013 T→C 10.9% G58G (GGA→GGG)  ddpB ← D‑ala‑D‑ala transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,555,0130TC10.9% 2332.1 / 38.6 889G58G (GGA→GGG) ddpBD‑ala‑D‑ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (462/285);  new base C (8/124);  total (477/412)
Fisher's exact test for biased strand distribution p-value = 2.80e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 900 total aligned reads displayed.

AGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTG  >  CP009273/1554864‑1555162
                                                                                                                                                     |                                                                                                                                                     
agGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGt                                                                                                                                                       >  2:16221/1‑150 (MQ=255)
agGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGt                                                                                                                                                       <  2:878790/150‑1 (MQ=255)
agGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGt                                                                                                                                                       >  1:870557/1‑150 (MQ=255)
  ggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTcc                                                                                                                                                     >  1:917790/1‑150 (MQ=255)
   gCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTccc                                                                                                                                                    <  2:465802/150‑1 (MQ=255)
     aCCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAg                                                                                                                                                  <  2:749333/150‑1 (MQ=255)
        aaaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTg                                                                                                                                               <  1:366973/150‑1 (MQ=255)
         aaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGc                                                                                                                                              <  1:135262/150‑1 (MQ=255)
          aaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCt                                                                                                                                             >  2:558081/1‑150 (MQ=255)
          aaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCt                                                                                                                                             >  1:1044661/1‑150 (MQ=255)
           aGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                                                                                                            <  2:575219/150‑1 (MQ=255)
           aGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                                                                                                            <  1:578631/150‑1 (MQ=255)
           aGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                                                                                                            <  2:108059/150‑1 (MQ=255)
             ccAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGc                                                                                                                                          <  2:239684/150‑1 (MQ=255)
               aGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                                                         >  2:854275/1‑149 (MQ=255)
                    cAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAatat                                                                                                                                    <  1:484716/149‑1 (MQ=255)
                    cAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAatat                                                                                                                                    <  1:471124/149‑1 (MQ=255)
                    cAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAata                                                                                                                                     <  1:1132767/148‑1 (MQ=255)
                     aGCCTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTCCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGCTCCAGCTGCTGGCGCATAatt                                                                                                                                  >  2:118635/1‑150 (MQ=255)
                      gCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATAtttt                                                                                                                                 <  2:375867/150‑1 (MQ=255)
                       cGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTc                                                                                                                                <  2:1056745/150‑1 (MQ=255)
                        gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTcc                                                                                                                               <  1:1022347/150‑1 (MQ=255)
                         tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                                                                                                              <  1:1002950/150‑1 (MQ=255)
                         tCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCa                                                                                                                              <  1:1102323/150‑1 (MQ=255)
                           gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACg                                                                                                                            <  2:393661/150‑1 (MQ=255)
                           gCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACg                                                                                                                            <  1:558081/150‑1 (MQ=255)
                             cGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGa                                                                                                                           >  1:802958/1‑149 (MQ=255)
                              gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAg                                                                                                                                                  <  1:32116/125‑1 (MQ=255)
                              gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGa                                                                                                                           >  1:987932/1‑148 (MQ=255)
                                gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCTGGCGAATATTTTCCACGATAg                                                                                                                        >  2:632380/1‑149 (MQ=255)
                                gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAg                                                                                                                        >  1:65708/1‑149 (MQ=255)
                                gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAg                                                                                                                        >  1:191111/1‑149 (MQ=255)
                                 aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCa                                                                                                                      <  2:594454/150‑1 (MQ=255)
                                    aaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCg                                                                                                                   <  1:1082010/150‑1 (MQ=255)
                                      tataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCt                                                                                                                 <  1:163767/150‑1 (MQ=255)
                                       ataCGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTg                                                                                                                >  1:516635/1‑150 (MQ=255)
                                          cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                                                              <  2:982117/149‑1 (MQ=255)
                                          cGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgc                                                                                                              <  1:969205/149‑1 (MQ=255)
                                           gCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGgcgc                                                                                                            <  2:150521/150‑1 (MQ=255)
                                            cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                                                                                                           <  2:802958/150‑1 (MQ=255)
                                                    ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCTGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAcc                                                                                                     <  1:442258/148‑1 (MQ=255)
                                                     cAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAAAGCATCGCTGGCGCGGTCACCCGc                                                                                                  >  2:207020/1‑150 (MQ=255)
                                                      aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCCGGCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCCCGGGCCCCCGcc                                                                                                 >  2:95438/1‑150 (MQ=255)
                                                       gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                <  2:83474/150‑1 (MQ=255)
                                                        cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg                                                                                               >  1:1067068/1‑150 (MQ=255)
                                                         cccGGACGCCCGGTACGAATGGATGTCCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGAAAAACTGTCCATACAGTGGCTGGCCCAGCCCCAGTTGCTGGCAAATATTTCCCACGATAGCACCGCTGGCGCGGTCACCCGCCAGt                                                                                              <  2:128704/149‑1 (MQ=255)
                                                                    ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCg                                                                                    >  1:501605/1‑149 (MQ=255)
                                                                    ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCg                                                                                    >  1:386353/1‑149 (MQ=255)
                                                                        cGAATGGATGTCCCCAGGTCACCATGAAACGGGTCGCTGACGTAGGGGAAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTCCCACGATAGCATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATc                                                                                <  1:115332/149‑1 (MQ=255)
                                                                         gAATGGATGTCCCCAGGTCACCAGAAAACAGGTCGCTGACGTAGGGGAAAAACTGTACATACAGGGGCTGGTCCAGCCCCAGTTGCGGGCGAATATTTCCCACGATAGCATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCAc                                                                              <  2:713490/150‑1 (MQ=255)
                                                                         aaggggATTTCCCCGGGTCACCAGGAAGCAGGTCGCTGTGGTAGCGATAAACCTAAACATGGGGGGGCTGCTCCGCTCCCGTTGGTTGGCGAATTTTTTCCAGGGTACCTGCGCTGGCGGGGCCCCCCGCCAGTAACCGCGCCGGATCAc                                                                              <  2:348567/146‑1 (MQ=255)
                                                                           agggATGTTCCCAGCTCACCATGAAACAGGCCGCTGCCGTGGGGAAAAACCTTTACATACAGTGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTCCCACGATACCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCa                                                                            <  2:293028/148‑1 (MQ=255)
                                                                            tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAg                                                                           >  2:73398/1‑150 (MQ=255)
                                                                            tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAg                                                                           >  1:699323/1‑150 (MQ=255)
                                                                              gatttccccGGGTCCCCATGAAACAGGTCGCGGACGGAGCGATAAACCTGTACATACAGTGGCTGGCCCGGTCCCAGTTGCTGGCAAATATTTCCCACAATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                         <  1:745878/144‑1 (MQ=255)
                                                                                tGTCCCCAGGTCACCATGAAACGGGTCGCTGACGTAGGGGTAAAACTGTACATACAGGGGCTGGTCCAGTCCGGGTTGCGGGCGAATATTTTCCCCGATAGCTTCGCTGGCGGGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                                                       <  1:944725/150‑1 (MQ=255)
                                                                                 gtccccAGGTCACAATGAAACGGGTCGCGGACGTAGGGGAAAAACTGTACATACAGTGGCTGGCCCAGCCCCGTTTGCTGGCAAATATTTCCCACGAAAGCATGGGGGGGGGGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                                                       <  2:648633/146‑1 (MQ=255)
                                                                                  ttCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGGCACCCGCCAGTAAACGCGCCGGATCACCAGGGAt                                                                       >  2:78777/1‑148 (MQ=255)
                                                                                     ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCAAATATTTCCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGAt                                                                  <  1:233601/150‑1 (MQ=255)
                                                                                     ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCAAATATTTCCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGAt                                                                  <  1:609397/150‑1 (MQ=255)
                                                                                       aGGTCACCATAAACCGGGCCGCTGACGTGGCGGTAAAACTGTACATACAGTGGTTGGCCCAGCCCGGGTTGCTGGCAAATTTTTCCCACGATAGCACCGCTGGCGCGGCCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGc                                                                <  2:324571/150‑1 (MQ=255)
                                                                                         gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCCGTCCCAGTTGGTGGTGAATATTTTCTACCATAGCCTCGCTTGCGCCGTCTCCCGGCAAAAAACGCCCCGGAACACCACGGACCAGATGc                                                                >  2:613185/1‑148 (MQ=255)
                                                                                            aCCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGGTCAGATGCgaga                                                            >  2:1010649/1‑149 (MQ=255)
                                                                                               aTGAAACGGATCGCTGACGTGGGGTTAAACCTTTACATACGGTGGCTGGCCCGGTCCCAGTTGCTGGGAAATATTTCCCACGATAGCACCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                                                        <  2:933204/150‑1 (MQ=255)
                                                                                               aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                         >  1:534796/1‑149 (MQ=255)
                                                                                                 gAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGGCCCAGTTGCTGGCGGATATTTTCCACGAAAGCATCGCTGGCGCGGTTACCCGCCCGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGgtaata                                                       >  1:682442/1‑149 (MQ=255)
                                                                                                 aaaccgggTCGCTGACGTAGCGGTAAAACTGTACATACGGTGGCTGGCCCGGCCCCAGTTGCTGGCGAATATTTCCCACGATAGCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGAtaataa                                                      <  2:218715/144‑1 (MQ=255)
                                                                                                  aaaCGGGTCGCTGACGTAGGGGTAAAACTGTACATACGGTGGTTGGCCCAGTCCGAGTTGCGGGCGAATATTTTCCAGAATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATaaa                                                     <  1:825708/150‑1 (MQ=255)
                                                                                                  aaaCGGGTCGCTGACGGAGCGGTAAAACTGTACATACGGTGGCTGGTCCAGCCCCGGTTGCTGGCAAATATTTCCCACGATAGCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGAtaataa                                                      <  1:90252/149‑1 (MQ=255)
                                                                                                  aaaCAGGCCGCTAGCGTGGGGGTAAAACTTTACATGCAGTGGTTGCCCCAGCCCCAGTTGCGGGCAAATTTTTCCCGGGATAGCAGCGCGGGCGGGTTCTCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATaaa                                                     <  2:422692/150‑1 (MQ=255)
                                                                                                   aaCAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAAc                                                    <  2:698525/150‑1 (MQ=255)
                                                                                                   aaCAGGTCGCTGACGTAGCGATAAAACTGTACATACGGTGGCTGGCCCAGCCCCAGTTGCTGGCAAATATTTTCCACGATAGCATCGCTGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAAc                                                    <  1:673820/150‑1 (MQ=255)
                                                                                                    acgggTCGCTGACGTAGGGGTAAAACTGTACATACAGTGGCTGGCCCAGCCCCGGTTGCTGGCAAATTTTTCCCACGATAGCACCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACg                                                   <  1:854275/147‑1 (MQ=255)
                                                                                                    acgggTCGCGGACGTGGGGGAAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCAAATATTTCCCACGATAGCATCGCTGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACg                                                   <  1:779359/147‑1 (MQ=255)
                                                                                                       ggTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGGTCAGATGCGAGATAATAAACGTaa                                                >  2:814634/1‑150 (MQ=255)
                                                                                                        gTCGCTGACGTGGCGTTAAAATTGTACATACAGTGGCTGGTCCAGTCCCATTGGCGGGCAAATTTTTTCCACGATAGCACCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAAt                                               <  2:1128097/150‑1 (MQ=255)
                                                                                                              gACGTGGCGGTAAAACTTAACAAACGGGGGTTGGCCCGGTCCCAGTGGCGGGCAAATTTTTCCCACGATACCATCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCa                                         <  2:268422/150‑1 (MQ=255)
                                                                                                              gACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACg                                           >  1:403773/1‑148 (MQ=255)
                                                                                                               aCGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACg                                           >  1:333329/1‑147 (MQ=255)
                                                                                                                cGTAGCGGAAAAACTGTCCATACGGGGGCTGCCCCAGCCCCAGTTGCGGGCGAATTTTTCCCACGATAGCATCGCGGGCGCGGCCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGa                                       <  2:681392/150‑1 (MQ=255)
                                                                                                                 gtgggggTAAAACTGAACATACGGTGTTTGGCCCGGCCCCAGTTGTGGGCAAATTTTTCCCAGAAGAGCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAc                                      <  2:517204/145‑1 (MQ=255)
                                                                                                                      ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAACGCTGGCGCGGTCACCCGCCAGGAAACGCGCCGGAACACCAGGGATCAGAAGCGAGATAATAAAAGTAATCACGCAGAcgcccg                                 >  2:407886/1‑150 (MQ=255)
                                                                                                                         aaaaCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGcca                              >  1:931798/1‑150 (MQ=255)
                                                                                                                          aaaCTGTACATACAGTGGCTGGGCCAGTCCCACCTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACCCCCGccac                             >  2:111282/1‑150 (MQ=255)
                                                                                                                            aCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGccacca                           >  1:723678/1‑150 (MQ=255)
                                                                                                                                 aaatacgGGGGCGGGCCCAGCCCCGGTTGGGGGAGAATTTTTCCCACGATAGCATCGCGGGCGGGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAg                       <  2:898158/147‑1 (MQ=255)
                                                                                                                                   atacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCAc                    >  2:490474/1‑150 (MQ=255)
                                                                                                                                     acaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCAc                    >  2:1035370/1‑148 (MQ=255)
                                                                                                                                         tGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGccc              >  2:44145/1‑150 (MQ=255)
                                                                                                                                          ggCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcccc             >  1:120200/1‑150 (MQ=255)
                                                                                                                                           gttGGCCCAGCCCAGGTTGCGGGAGAATATTTCCCACGATAGCACCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGccccc            <  1:599437/148‑1 (MQ=255)
                                                                                                                                             tGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcag          >  1:641265/1‑150 (MQ=255)
                                                                                                                                             gggcccAGTCCCTGTTGCTGCGAAATTTTTCCCAGGATACCACCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcag          <  1:842561/146‑1 (MQ=255)
                                                                                                                                              ggTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcag          >  1:214909/1‑149 (MQ=255)
                                                                                                                                                tCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcagcag       >  1:292863/1‑150 (MQ=255)
                                                                                                                                                tCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcagcag       >  2:184540/1‑150 (MQ=255)
                                                                                                                                                 ccAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCagcagc      >  2:1095237/1‑150 (MQ=255)
                                                                                                                                                 ccAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCagcagc      >  2:1150644/1‑150 (MQ=255)
                                                                                                                                                  cAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCg     >  1:268374/1‑150 (MQ=255)
                                                                                                                                                   aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt    >  1:42724/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTG  >  CP009273/1554864‑1555162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑