| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,145,170 | G→T | 35.1% | intergenic (+43/+22) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,145,170 | 0 | G | T | 35.1% | 29.3 / 10.9 | 51 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (20/12); new base T (10/8); total (31/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.77e-01 | |||||||||||
TATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCG > CP009273/2145023‑2145316 | tataCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTc < 2:143667/150‑1 (MQ=255) aaaTTTACGGCGGTTATACAATTGATCTTTTATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGAAAAGAGAAAAATTAG‑TTCGCTAACCAGCCGTTATTTTTATTGTTAAACCGGGGGG‑TTGAATTGTTGTCTTCAACCGCCATgaga < 2:140136/150‑1 (MQ=255) ggcggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTAATTTTTGTCTTTCACCGCCCTGAGAAaatcact > 1:1025148/1‑150 (MQ=255) cggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTAATTGTTGTTTTCAACCGCCATGAGAAACTCActct > 2:1140426/1‑150 (MQ=255) cggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTCAATTGTTGGTTTTATCCCCCCTGAGAAACTCActct > 2:834388/1‑150 (MQ=255) ggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTg > 1:476866/1‑150 (MQ=255) gTTATACCATTGATCTTTTATTGGTCACATATCAAAAAGAAAAAGCCAACGAGAAAAAAGAAAAATTAG‑TTCGAAAACAAGCAGTTATATTAATTTTTAAAACGGGCGG‑TTGATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGc < 1:203899/150‑1 (MQ=255) gTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTCTTCAACCGCCATGAGAAAATCACCCTGc > 1:856586/1‑150 (MQ=255) aCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTTTTTTTTTCAACCGCCATGAGAAAATCACTCTGCCcac > 2:839504/1‑149 (MQ=255) cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAgc > 2:173378/1‑150 (MQ=255) cATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTTTTCTACCCCCCTGAGAAAATCACTCTGccccccgc > 2:346803/1‑145 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTGATTGTTGTCCTCCACCACCATGTGAAACACACTCTGCCCACAgcga > 2:234404/1‑148 (MQ=255) gATCTTTTTTTGCTCCCCTATCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAAATTAG‑TTCTCTAATCAGCCTTTATTTTTATTTTTAAAACGGGCGG‑TTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc < 2:624299/149‑1 (MQ=255) aTTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTTTTCAACCGCCCTTAGAAAATCACTCTGCCCCCAGCGCCCGTGCCAAc > 2:1044760/1‑150 (MQ=255) aTTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAAc > 1:971076/1‑150 (MQ=255) ttgtcccccTAACAAAAAAAAGAGGCCGACGAGCTAAGAGAAAAATTAT‑TTCGCTAATCGCCCTTTATTTTTATTGTTAAACCTGGCGG‑TTGAATTGTTTTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACg < 2:731654/143‑1 (MQ=255) aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTat > 1:1004128/1‑150 (MQ=255) ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTat > 1:606401/1‑149 (MQ=255) ccTATCAAAAAGAAGAAGCAGACGAGATAAGAGAAAAATTAT‑TTCGATAATCGGCTTTTATATTTATTGTTAAACCGGGGGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatc < 2:693074/150‑1 (MQ=255) agaagaagCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTAATTGTTGTCTTCAACCGCCATGAGAAAATCCCTCTGCCCACAGCGCCAGTGCCAACGCTTTTTCATCCTCCGt > 1:872388/1‑149 (MQ=255) agaagaagCCGACGAGCAAAAAAAAAAATTAG‑TTCGAAAATCAGCTTTTATATTTATTGTTAAAACGGGGGG‑TTAAATTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTa < 2:774071/150‑1 (MQ=255) gaggaggCCGACGAAAAAAGAGAAAAATTAT‑TTCACTATTCAGCCTTTATATTTTTTGTTTAAACGGGGGG‑TTGATTTGTTGTTTTCAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc < 2:33163/144‑1 (MQ=255) aagaagCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑GTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg > 2:14357/1‑150 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCCGTGCCAACGGTTTATCATCATCCGTACGc > 1:446662/1‑150 (MQ=255) aagCCGAGGAGCAAAAAAAAAAATTAT‑TTCGTTAATCACCGTTTATTTTTTTTGTTAAAACGGGCGT‑TTAAATTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTc < 2:881234/150‑1 (MQ=255) aagCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAACGCCAGTGCCAACGGTTTATCATCATCCGTACGCTc > 1:876550/1‑150 (MQ=255) ccGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGtt > 2:213716/1‑150 (MQ=255) ccGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTCTCCTCAACCGCCATGAGAAAATCCCTCTCCCCACCGCCCCAATGCCAACGCCTTATCATTTTCCGTACCCTCgat > 1:57248/1‑148 (MQ=255) cgaGCTAAGAGAAAATTTAT‑TCCGTTAATCACCCTTTATTTTTATTTTTTAAACGGGGGT‑TTAATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAcc < 1:170678/150‑1 (MQ=255) gaGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTTTCATCATCCCTACCCCCGTTTAccg > 2:425972/1‑150 (MQ=255) gCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTCTTTAACCGCCATTAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTAccgcc > 1:399957/1‑150 (MQ=255) taaagaaaaaaTTTGTTTCGATATTCAGCTTTTATTTTTTTTTTTAAAACGGGGGT‑TTGATTTTTTGTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccgccg < 1:253908/143‑1 (MQ=255) tAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCgg > 2:400146/1‑150 (MQ=255) agagCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTTTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCCTACGCCCGTTTACCGCCGGGc > 2:431716/1‑150 (MQ=255) gagCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCt > 1:376525/1‑150 (MQ=255) gCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAAACGCCATGAGAAACTCACTTTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTgg > 1:1000980/1‑150 (MQ=255) aaaaTTAT‑TTCGTTAATCCGCCTTTATTTTTTTTGTTAAACCTGGGGT‑TTAAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGAc < 1:716363/150‑1 (MQ=255) aaaaTTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTTTTCCACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCCGTGCCAACGTTTTTTCATCATCCGCACCCCCGTTTACCCCCCGGCCGGa > 1:487349/1‑149 (MQ=255) aaaTTAT‑TCCGCTACTCACCCTTTTTATTT‑TTTTTTAAACCGGGGGTTTGATTTTTTTCCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACa < 1:997273/150‑1 (MQ=255) aG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTTAATTGTTGTCTTCAAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTTGGCaaaaaa > 1:660163/1‑149 (MQ=255) tttCTCTAATCAGCCTTTATTTTTATTTTTAAAACGGGCGT‑TTAAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAc < 2:164570/148‑1 (MQ=255) cTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTCTTTAAACGCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAAAAGCttt > 1:1190264/1‑150 (MQ=255) aaTCAGCTTTTTTTTTTTTTTTTAAAACGGGGGT‑TTAATTTTTTTTTTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTa < 1:1044760/149‑1 (MQ=255) aaTCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTTTTTTCTTCAACCCCCATTAGAAAATCACTCTGCCCCCCGCCCCCGTGCCCACGGTTTTTTATCATCCGTACGCCCGTTTTCCGCCCGGCTGGGCAAAAACTGCtttt > 2:587407/1‑148 (MQ=255) aTCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATTATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCtttta > 1:1103777/1‑147 (MQ=255) tataTTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCCGGCTGGACAAAAACTGCTTTTACAATTCAgg > 2:294495/1‑149 (MQ=255) attttttttttttAAACGGGCGG‑TTGATTTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGt < 2:476866/146‑1 (MQ=255) tattttttTTTAAAACGGGGGT‑TTAATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta < 2:446662/149‑1 (MQ=255) ttattTTTTAAACGGGCGG‑TTAAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata < 1:511822/149‑1 (MQ=255) taaaaCGGGGGT‑TTGATTTTTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg < 2:561505/147‑1 (MQ=255) accgggggg‑TTGATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgt < 1:1123577/143‑1 (MQ=255) ggtgtttttgttcTTTTCAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg < 1:346803/143‑1 (MQ=255) ttgttgTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc > 2:17109/1‑150 (MQ=255) ttgttgTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc > 1:1151904/1‑150 (MQ=255) gttgtCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgccg > 1:1159955/1‑150 (MQ=255) | TATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAG‑TTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGG‑TTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCG > CP009273/2145023‑2145316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |