Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,666 A→T 68.8% A143A (GCA→GCT)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,6660AT68.8% 347.0 / 231.6 218A143A (GCA→GCT) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base A (38/30);  new base T (83/67);  total (121/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.73e-05

Only 100 of 219 total aligned reads displayed.

AACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGA  >  CP009273/8519‑8814
                                                                                                                                                   |                                                                                                                                                    
aaCTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCa                                                                                                                                                      <  2:1095627/148‑1 (MQ=255)
   cgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACa                                                                                                                                                 <  1:1054298/149‑1 (MQ=255)
     aaGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACa                                                                                                                                                 <  2:272108/148‑1 (MQ=255)
          gATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGa                                                                                                                                           <  1:824078/149‑1 (MQ=255)
          gATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGa                                                                                                                                           <  1:760344/149‑1 (MQ=255)
              cgcgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGaac                                                                                                                                         >  1:514793/1‑146 (MQ=255)
                cgTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAaagaa                                                                                                                                    <  1:442801/150‑1 (MQ=255)
                    tttCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCa                                                                                                                                >  1:1123272/1‑150 (MQ=255)
                       ccTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATc                                                                                                                              <  1:1190830/149‑1 (MQ=255)
                        cTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTaa                                                                                                                            >  1:128466/1‑150 (MQ=255)
                           tGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACt                                                                                                                          <  2:461781/149‑1 (MQ=255)
                           tGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACt                                                                                                                          <  1:738123/149‑1 (MQ=255)
                               acCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAAc                                                                                                                     >  2:65836/1‑150 (MQ=255)
                                 cGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCt                                                                                                                   >  2:634668/1‑150 (MQ=255)
                                    aGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGAc                                                                                                                <  2:566252/150‑1 (MQ=255)
                                     gCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGAcg                                                                                                               >  1:145790/1‑149 (MQ=255)
                                      cGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAaagaag                                                                                                                                   <  1:615135/129‑1 (MQ=255)
                                      cGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGAcgc                                                                                                              >  1:1188199/1‑148 (MQ=255)
                                       gTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGct                                                                                                             >  2:594395/1‑150 (MQ=255)
                                            tcgCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACgctgct                                                                                                          <  1:854300/146‑1 (MQ=255)
                                              gCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGtt                                                                                                       <  2:334748/149‑1 (MQ=255)
                                              gCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGtt                                                                                                       <  2:793634/149‑1 (MQ=255)
                                              gCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTtc                                                                                                      <  2:1062291/150‑1 (MQ=255)
                                              gCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTtc                                                                                                      >  1:624994/1‑150 (MQ=255)
                                               cGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTtct                                                                                                     >  2:86830/1‑150 (MQ=255)
                                                 aaaGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTcc                                                                                                   <  2:1158850/150‑1 (MQ=255)
                                                 aaaGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTcc                                                                                                   <  2:1165176/150‑1 (MQ=255)
                                                  aaGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCt                                                                                                  >  1:1052632/1‑150 (MQ=255)
                                                  aaGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCt                                                                                                  >  1:288324/1‑150 (MQ=255)
                                                  aaGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCt                                                                                                  <  2:347520/150‑1 (MQ=255)
                                                   aGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCtt                                                                                                 >  1:253992/1‑150 (MQ=255)
                                                   aGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCtt                                                                                                 >  2:843681/1‑150 (MQ=255)
                                                   aGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCtt                                                                                                 <  1:638071/150‑1 (MQ=255)
                                                       aaaCGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCt                                                                                             <  2:61672/150‑1 (MQ=255)
                                                          cGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAg                                                                                          >  2:764654/1‑150 (MQ=255)
                                                           gcatcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAgg                                                                                         <  2:139585/145‑1 (MQ=255)
                                                           gcatcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAgg                                                                                         <  1:586931/145‑1 (MQ=255)
                                                           gcatcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAgg                                                                                         >  2:434529/6‑150 (MQ=255)
                                                             atcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCt                                                                                       <  2:933587/147‑1 (MQ=255)
                                                             atcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCt                                                                                       >  2:805406/4‑150 (MQ=255)
                                                              tcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGTAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTc                                                                                      <  1:770747/148‑1 (MQ=255)
                                                                   aagctgtACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCt                                                                                 <  1:545735/144‑1 (MQ=255)
                                                                      ctgtACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTtgc                                                                              <  2:526665/147‑2 (MQ=255)
                                                                       tgtACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTtgcg                                                                             >  2:816743/3‑148 (MQ=255)
                                                                         tACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgcc                                                                           >  1:710760/1‑149 (MQ=255)
                                                                           cAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCAGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGc                                                                            <  2:157184/147‑1 (MQ=255)
                                                                           cAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGa                                                                         <  2:374581/150‑1 (MQ=255)
                                                                            aaCGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgccg                                                                          >  2:1092953/1‑146 (MQ=255)
                                                                              cGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGc                                                                      <  1:641759/150‑1 (MQ=255)
                                                                                     ggcatcaGCAATGACCGCATTCTGATCCAACTGGCCTCCACCTGGTAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTTACGCTGCTGTTCTCCTTCACTCAGGCTCGCGCTTGCGCCGAAGCGGGCgtc                                                               >  2:901266/7‑149 (MQ=255)
                                                                                     ggcatcaGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCgtc                                                               >  1:920784/7‑149 (MQ=255)
                                                                                     ggTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGc                                                                            <  2:448338/137‑1 (MQ=255)
                                                                                      gTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAACAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCgt                                                                <  2:514793/148‑1 (MQ=255)
                                                                                       tATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGtt                                                             >  2:977741/1‑150 (MQ=255)
                                                                                        gtcaGCAACGATCGCATTCTGATCAAACTGGCCACCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                            >  2:358494/4‑150 (MQ=255)
                                                                                        atcaGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                            >  1:254659/4‑150 (MQ=255)
                                                                                        accaGCAACGATCGCATTCCGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGCAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                            >  2:659646/4‑150 (MQ=255)
                                                                                           aGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGGGCCGAAGCGGGCGTCTTCCt                                                          >  1:99160/1‑149 (MQ=255)
                                                                                            gCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTg                                                         <  1:594738/149‑1 (MQ=255)
                                                                                             cAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCtt                                                             >  1:1096672/1‑144 (MQ=255)
                                                                                             cAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAt                                                       <  2:667618/150‑1 (MQ=255)
                                                                                              aaCGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAtt                                                      <  1:661181/150‑2 (MQ=255)
                                                                                              aaCGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAtt                                                      <  2:1022517/150‑2 (MQ=255)
                                                                                               aCGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAttt                                                     >  1:183079/1‑148 (MQ=255)
                                                                                               aCGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAttt                                                     <  2:38731/150‑3 (MQ=255)
                                                                                                cGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAtttc                                                    >  2:228211/1‑150 (MQ=255)
                                                                                                cGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAtttc                                                    >  1:321319/1‑150 (MQ=255)
                                                                                                 gATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCg                                                   <  1:493849/150‑1 (MQ=255)
                                                                                                 gATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCg                                                   >  2:215179/1‑150 (MQ=255)
                                                                                                 gATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCg                                                   >  2:730772/1‑150 (MQ=255)
                                                                                                   tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                                 >  2:394302/1‑150 (MQ=255)
                                                                                                   tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                                 <  1:1156729/150‑1 (MQ=255)
                                                                                                   tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                                 <  2:536846/150‑1 (MQ=255)
                                                                                                   tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                                 <  1:580567/150‑1 (MQ=255)
                                                                                                    cgcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCg                                                >  2:552482/4‑150 (MQ=255)
                                                                                                     gcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGt                                               >  1:747628/3‑150 (MQ=255)
                                                                                                     gcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGt                                               <  2:42966/148‑1 (MQ=255)
                                                                                                     gTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCg                                                <  1:379514/149‑1 (MQ=255)
                                                                                                         tCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTtcgt                                           >  2:743061/1‑150 (MQ=255)
                                                                                                            gATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTgg                                        >  1:458366/1‑150 (MQ=255)
                                                                                                             aTCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGc                                       >  2:253196/1‑150 (MQ=255)
                                                                                                             aTCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCACAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCcgttcgtt                                          <  2:1052632/147‑1 (MQ=255)
                                                                                                                   cTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTAtc                                 <  1:531109/150‑2 (MQ=255)
                                                                                                                    tGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTc                                >  2:467082/1‑150 (MQ=255)
                                                                                                                      gcctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCt                               <  2:292887/143‑1 (MQ=255)
                                                                                                                       cctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtc                              >  2:120225/6‑148 (MQ=255)
                                                                                                                        ctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTtcgt                                           >  1:401349/5‑135 (MQ=255)
                                                                                                                        ctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGa                            >  1:227386/5‑150 (MQ=255)
                                                                                                                          cTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACt                          >  1:695746/1‑150 (MQ=255)
                                                                                                                           caCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACt                          <  2:499941/148‑1 (MQ=255)
                                                                                                                             ccTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTgg                        >  1:823405/1‑149 (MQ=255)
                                                                                                                              cTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTa                      >  2:946555/1‑150 (MQ=255)
                                                                                                                               tGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTa                      >  1:385555/1‑149 (MQ=255)
                                                                                                                               tGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTa                      >  1:390896/1‑149 (MQ=255)
                                                                                                                                 gCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACa                    <  1:434529/149‑1 (MQ=255)
                                                                                                                                   aGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAg                 <  2:535012/150‑1 (MQ=255)
                                                                                                                                     ggTATCCGTGATGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCg               <  1:1007829/150‑1 (MQ=255)
                                                                                                                                            gTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATAcc         >  2:717136/1‑149 (MQ=255)
                                                                                                                                              gctgcAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGa       >  1:555750/1‑149 (MQ=255)
                                                                                                                                                  cAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATaaga  <  1:1089560/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGA  >  CP009273/8519‑8814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑