Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,804 T→C 74.1% N189N (AAT→AAC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,8040TC74.1% 410.8 / 181.7 239N189N (AAT→AAC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (34/28);  new base C (94/83);  total (128/111)
Fisher's exact test for biased strand distribution p-value = 8.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-03

Only 100 of 239 total aligned reads displayed.

CCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCT  >  CP009273/8657‑8952
                                                                                                                                                   |                                                                                                                                                    
ccGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATAc                                                                                                                                                    >  2:336888/1‑150 (MQ=255)
  gTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATAcc                                                                                                                                                   >  2:717136/1‑149 (MQ=255)
    gctgcAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGa                                                                                                                                                 >  1:555750/1‑149 (MQ=255)
      tgcAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGAt                                                                                                                                                >  2:905955/1‑148 (MQ=255)
        cAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATaaga                                                                                                                                            <  1:1089560/150‑1 (MQ=255)
           aaCAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaagaaag                                                                                                                                         <  1:52560/150‑1 (MQ=255)
            aCAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaagaaaga                                                                                                                                        <  2:1124350/150‑1 (MQ=255)
            aCAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaagaaa                                                                                                                                          >  1:276299/1‑148 (MQ=255)
             cAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                                                                                                       <  2:811658/150‑1 (MQ=255)
             cAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                                                                                                       >  1:331839/1‑150 (MQ=255)
              aGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                                                                                                       >  2:987068/1‑149 (MQ=255)
               gCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTa                                                                                                                                     <  2:601115/150‑1 (MQ=255)
                cTGGAAAAAGTAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTAc                                                                                                                                    <  1:248138/150‑1 (MQ=255)
                cTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTAc                                                                                                                                    <  2:641708/150‑1 (MQ=255)
                     aaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                                                                                               <  2:1028648/150‑1 (MQ=255)
                       aagaagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCgg                                                                                                                             <  1:743061/150‑1 (MQ=255)
                          aagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCgg                                                                                                                             >  2:496012/1‑147 (MQ=255)
                           agGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                          >  1:1073647/1‑149 (MQ=255)
                             gCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCagaag                                                                                                                       <  1:549874/150‑1 (MQ=255)
                             gCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                          >  2:730865/1‑147 (MQ=255)
                                ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATc                                                                                                                    <  1:354409/148‑1 (MQ=255)
                                 cAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATcc                                                                                                                   >  2:1132250/1‑150 (MQ=255)
                                  aaCTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATcc                                                                                                                   <  1:659249/149‑1 (MQ=255)
                                   aCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCg                                                                                                                  <  1:171323/149‑1 (MQ=255)
                                   aCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCg                                                                                                                  <  2:785605/149‑1 (MQ=255)
                                    cTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCggg                                                                                                                >  1:763980/1‑150 (MQ=255)
                                       tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGt                                                                                                             >  1:709061/1‑150 (MQ=255)
                                         aCCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTg                                                                                                            <  1:253196/149‑1 (MQ=255)
                                         aCCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTg                                                                                                            <  1:81306/149‑1 (MQ=255)
                                         aCCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTg                                                                                                            <  1:464164/149‑1 (MQ=255)
                                          ccTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGt                                                                                                          >  1:1173855/1‑150 (MQ=255)
                                           cTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGtt                                                                                                         <  1:905955/150‑1 (MQ=255)
                                            tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                        >  1:1178690/1‑150 (MQ=255)
                                                gctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGt                                                                                                    >  2:1062806/2‑150 (MQ=255)
                                                    ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagc                                                                                                <  1:1016335/150‑5 (MQ=255)
                                                    ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagc                                                                                                >  1:1149344/1‑146 (MQ=255)
                                                    ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagc                                                                                                >  2:836858/1‑146 (MQ=255)
                                                            cTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTa                                                                                        >  1:60653/1‑150 (MQ=255)
                                                             ttCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTAc                                                                                       >  2:593328/1‑150 (MQ=255)
                                                               cGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTAc                                                                                       <  1:597106/148‑1 (MQ=255)
                                                                 cTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAATACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAg                                                                                    >  1:255664/1‑149 (MQ=255)
                                                                  tCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGa                                                                                              >  2:635347/1‑138 (MQ=255)
                                                                   cAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGaa                                                                                             <  1:635347/138‑1 (MQ=255)
                                                                    aGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGtat                                                                                 >  1:785858/1‑148 (MQ=255)
                                                                       cTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGtattac                                                                              >  2:238509/1‑149 (MQ=255)
                                                                       cTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACa                                                                             <  1:125370/150‑1 (MQ=255)
                                                                          gTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTTCCAGTACTACAAag                                                                          <  1:921541/150‑1 (MQ=255)
                                                                            gCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACaagcag                                                                        >  1:732277/1‑146 (MQ=255)
                                                                                gcgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACg                                                                    >  2:1119387/6‑150 (MQ=255)
                                                                                   ccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACggct                                                                 <  2:707731/148‑3 (MQ=255)
                                                                                    cgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTa                                                                >  1:205194/2‑150 (MQ=255)
                                                                                     gAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTAt                                                               <  2:288324/150‑1 (MQ=255)
                                                                                       aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATg                                                              >  2:458976/1‑149 (MQ=255)
                                                                                         cGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGaaa                                                           >  2:932017/1‑150 (MQ=255)
                                                                                         cGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGaaa                                                           <  2:1188199/150‑1 (MQ=255)
                                                                                          gggCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAAc                                                          >  2:58966/1‑150 (MQ=255)
                                                                                          gggCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAAc                                                          >  1:427219/1‑150 (MQ=255)
                                                                                           ggCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAAcc                                                         <  2:685668/150‑1 (MQ=255)
                                                                                            gCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCg                                                        <  1:633215/150‑1 (MQ=255)
                                                                                             cGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGt                                                       <  2:254516/150‑1 (MQ=255)
                                                                                             cGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGt                                                       <  2:535215/150‑1 (MQ=255)
                                                                                              gtgtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTg                                                      <  2:769300/150‑1 (MQ=255)
                                                                                              gtgtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTg                                                      >  1:300811/1‑150 (MQ=255)
                                                                                                cttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgt                                                    >  1:1054570/2‑150 (MQ=255)
                                                                                                 ttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgt                                                    >  1:896688/1‑149 (MQ=255)
                                                                                                  tCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTa                                                  >  1:1128292/1‑150 (MQ=255)
                                                                                                   ccTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgtga                                                  <  1:496012/149‑3 (MQ=255)
                                                                                                    cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATg                                                <  2:176279/150‑1 (MQ=255)
                                                                                                     tGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATgg                                               >  2:847494/1‑150 (MQ=255)
                                                                                                     tGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATgg                                               >  2:1097408/1‑150 (MQ=255)
                                                                                                       atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgc                                             <  2:390896/147‑1 (MQ=255)
                                                                                                       atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgc                                             >  1:138870/4‑150 (MQ=255)
                                                                                                        tttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGAt                                                 >  2:873334/3‑145 (MQ=255)
                                                                                                         ttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATg                                                <  1:873334/144‑1 (MQ=255)
                                                                                                            gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAg                                        <  2:450813/150‑1 (MQ=255)
                                                                                                            gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAg                                        >  1:51475/1‑150 (MQ=255)
                                                                                                              cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                      >  2:1000323/1‑150 (MQ=255)
                                                                                                                 tcgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTcc                                   >  2:408008/3‑150 (MQ=255)
                                                                                                                   gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                  <  1:98767/149‑1 (MQ=255)
                                                                                                                   gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                  <  2:1178690/149‑1 (MQ=255)
                                                                                                                   gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                  >  1:22507/1‑149 (MQ=255)
                                                                                                                        ccGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACAt                            <  1:58966/150‑1 (MQ=255)
                                                                                                                        ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                            >  2:753172/1‑148 (MQ=255)
                                                                                                                        ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                            <  2:128466/150‑3 (MQ=255)
                                                                                                                          gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                          <  1:932253/150‑5 (MQ=255)
                                                                                                                               ctcgACTGGTACAAAGCCAACACCGACAAGACAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGa                      >  2:1057865/4‑149 (MQ=255)
                                                                                                                               ctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGa                      >  1:363915/4‑149 (MQ=255)
                                                                                                                                  gACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGaa                     <  1:838355/147‑1 (MQ=255)
                                                                                                                                  gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAAtt                  <  1:676016/150‑1 (MQ=255)
                                                                                                                                  gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAAt                   <  1:1032982/149‑1 (MQ=255)
                                                                                                                                   aCTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTc                 <  2:991476/150‑1 (MQ=255)
                                                                                                                                   aCTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTc                 <  2:1073647/150‑1 (MQ=255)
                                                                                                                                         aCAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc           >  2:188350/1‑150 (MQ=255)
                                                                                                                                         aCAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc           >  1:1000771/1‑150 (MQ=255)
                                                                                                                                          cAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACt          >  1:733841/1‑150 (MQ=255)
                                                                                                                                          cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc           <  1:1062806/149‑1 (MQ=255)
                                                                                                                                          cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt          <  2:652283/150‑1 (MQ=255)
                                                                                                                                           aaaGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTg         <  1:849238/150‑1 (MQ=255)
                                                                                                                                            aaGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTgg        >  2:788510/1‑150 (MQ=255)
                                                                                                                                                  aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCt  >  2:955863/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCT  >  CP009273/8657‑8952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑