BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,480	Δ1 bp	100%	coding (1/963 nt)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,480	0	A	.	100.0%	1225.8 / NA	249	coding (1/963 nt)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (142/107); total (142/107)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

TAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGAC  >  CP009273/4097336‑4097623

taAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTt                                                                                                                                           <  1:759956‑M1/150‑2 (MQ=255)

taAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTt                                                                                                                                           >  2:30594‑M1/1‑149 (MQ=255)

taAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATT                                                                                                                                            <  1:932655‑M1/149‑1 (MQ=255)

  aaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtc                                                                                                                                          <  2:657187‑M1/149‑3 (MQ=255)

        cGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaa                                                                                                                                     >  1:859524‑M1/1‑141 (MQ=255)

         gCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaag                                                                                                                                  >  1:1407075‑M1/1‑140 (MQ=255)

         gCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaag                                                                                                                                  >  2:446474‑M1/1‑140 (MQ=255)

          ccATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                 <  2:818311‑M1/150‑12 (MQ=255)

           cATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggc                                                                                                                                <  1:796769‑M1/150‑13 (MQ=255)

           cATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                 >  2:553842‑M1/1‑138 (MQ=255)

           cATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                 >  1:343761‑M1/1‑138 (MQ=255)

            aTTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                 >  1:550152‑M1/1‑137 (MQ=255)

                ggCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagat                                                                                                                            <  1:767633‑M1/149‑17 (MQ=255)

                   ctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcct                                                                                                                        >  2:6672‑M1/1‑130 (MQ=255)

                   ctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcct                                                                                                                        >  1:145979‑M1/1‑130 (MQ=255)

                      acctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattccttta                                                                                                                     <  2:1380765‑M1/150‑24 (MQ=255)

                        ctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttacc                                                                                                                   >  2:35450‑M1/1‑125 (MQ=255)

                         tgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccc                                                                                                                  >  1:840258‑M1/1‑124 (MQ=255)

                         tgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccc                                                                                                                  >  2:1364044‑M1/1‑124 (MQ=255)

                           aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                 >  2:233403‑M1/1‑122 (MQ=255)

                            aTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctg                                                                                                                >  1:291932‑M1/1‑121 (MQ=255)

                            aTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctg                                                                                                                >  1:117469‑M1/1‑121 (MQ=255)

                               aaTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaac                                                                                                            >  1:1207346‑M1/1‑118 (MQ=255)

                               aaTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaa                                                                                                             <  1:984762‑M1/149‑32 (MQ=255)

                                aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                           <  1:275247‑M1/150‑34 (MQ=255)

                                aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                           >  1:634876‑M1/1‑117 (MQ=255)

                                    agcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                        >  2:174264‑M1/1‑113 (MQ=255)

                                     gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                      <  2:766799‑M1/150‑39 (MQ=255)

                                     gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                      >  2:520641‑M1/1‑112 (MQ=255)

                                     gcagGAAGTGATTGTTATACTAGTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                      <  2:1118521‑M1/150‑39 (MQ=255)

                                      cagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                      >  1:110567‑M1/1‑111 (MQ=255)

                                        ggAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                      >  2:743395‑M1/1‑109 (MQ=255)

                                         gAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacag                                                                                                   >  1:501811‑M1/1‑108 (MQ=255)

                                          aaGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacag                                                                                                   >  2:70129‑M1/1‑107 (MQ=255)

                                          aaGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacag                                                                                                   >  1:725146‑M1/1‑107 (MQ=255)

                                          aaGTAATTGTTATACTATTTGCACATTCGTTGGATTATTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggataaaggcagattcctttaccctgaacccgatgacag                                                                                                   >  2:1410392‑M1/1‑107 (MQ=255)

                                           aGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                                >  2:1201598‑M1/1‑106 (MQ=255)

                                            gTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                                >  1:405128‑M1/1‑105 (MQ=255)

                                             tGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagca                                                                                              <  1:1006142‑M1/150‑47 (MQ=255)

                                               aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaa                                                                                             <  2:105919‑M1/149‑48 (MQ=255)

                                                 tGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaa                                                                                          >  2:373668‑M1/1‑100 (MQ=255)

                                                     ataCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcc                                                                                      >  1:1462013‑M1/1‑96 (MQ=255)

                                                     ataCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcc                                                                                      >  1:90127‑M1/1‑96 (MQ=255)

                                                      taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcct                                                                                     >  2:1240615‑M1/1‑95 (MQ=255)

                                                        cTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctga                                                                                   >  2:1247853‑M1/1‑93 (MQ=255)

                                                         tATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgat                                                                                  >  1:1301281‑M1/1‑92 (MQ=255)

                                                           tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgat                                                                                  <  2:412485‑M1/148‑59 (MQ=255)

                                                           tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCACTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgc                                                                                >  1:215074‑M1/1‑90 (MQ=255)

                                                            ttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcg                                                                               >  2:1018435‑M1/1‑89 (MQ=255)

                                                            ttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcg                                                                               >  2:872885‑M1/1‑89 (MQ=255)

                                                            ttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcg                                                                               >  2:98349‑M1/1‑89 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                            >  1:1134064‑M1/1‑86 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                                >  1:1094066‑M1/1‑86 (MQ=255)

                                                                acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttc                                                                           <  2:27719‑M1/150‑66 (MQ=255)

                                                                acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttc                                                                           <  1:378100‑M1/150‑66 (MQ=255)

                                                                acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttc                                                                           >  1:555424‑M1/1‑85 (MQ=255)

                                                                acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagc                                                                                               <  2:1094066‑M1/130‑46 (MQ=255)

                                                                 caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcg                                                                          <  1:774274‑M1/150‑67 (MQ=255)

                                                                 caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcg                                                                          <  1:1326206‑M1/150‑67 (MQ=255)

                                                                      gTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttat                                                                     <  1:1035263‑M1/150‑72 (MQ=255)

                                                                      gTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttat                                                                     >  1:1365860‑M1/1‑79 (MQ=255)

                                                                        tGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttat                                                                     >  2:1188843‑M1/1‑77 (MQ=255)

                                                                          gATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcagg                                                                 <  2:74020‑M1/150‑76 (MQ=255)

                                                                          gATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcag                                                                  <  1:1273956‑M1/149‑75 (MQ=255)

                                                                           aTCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggc                                                                <  1:672015‑M1/150‑77 (MQ=255)

                                                                            tCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcc                                                               >  2:495159‑M1/1‑73 (MQ=255)

                                                                            tCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcc                                                               <  1:1012019‑M1/150‑78 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggccta                                                             >  1:244311‑M1/1‑71 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcct                                                              <  1:1192517‑M1/149‑79 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcct                                                              <  1:893008‑M1/149‑79 (MQ=255)

                                                                                ttCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctaca                                                           >  2:1289027‑M1/1‑69 (MQ=255)

                                                                                  cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacat                                                          <  2:1031901‑M1/149‑83 (MQ=255)

                                                                                     tgtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaat                                                      <  1:1018435‑M1/150‑87 (MQ=255)

                                                                                             aagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaat                                              <  2:725146‑M1/150‑95 (MQ=255)

                                                                                             aagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaat                                              >  1:1260581‑M1/1‑56 (MQ=255)

                                                                                             aagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaat                                              >  1:565653‑M1/1‑56 (MQ=255)

                                                                                              agaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatt                                             >  1:641616‑M1/1‑55 (MQ=255)

                                                                                                 cTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttat                                          >  1:185409‑M1/1‑52 (MQ=255)

                                                                                                    ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattg                                        <  1:702962‑M1/149‑101 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                     >  2:52810‑M1/1‑47 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                     >  1:328876‑M1/1‑47 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                     >  1:744115‑M1/1‑47 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                     <  2:1306850‑M1/150‑104 (MQ=255)

                                                                                                        cAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaattt                                   <  2:967050‑M1/150‑106 (MQ=255)

                                                                                                        cAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaattt                                   >  1:31427‑M1/1‑45 (MQ=255)

                                                                                                          aCAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgc                                 <  1:531410‑M1/150‑108 (MQ=255)

                                                                                                              aTTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaac                             >  1:161314‑M1/1‑39 (MQ=255)

                                                                                                                 tCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactt                           <  1:1052415‑M1/149‑114 (MQ=255)

                                                                                                                  cATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttt                          <  2:1407075‑M1/149‑115 (MQ=255)

                                                                                                                   aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttg                        <  2:422099‑M1/150‑117 (MQ=255)

                                                                                                                              cAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggat              <  2:1011086‑M1/149‑127 (MQ=255)

                                                                                                                                aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggat              >  1:452133‑M1/1‑21 (MQ=255)

                                                                                                                                 aGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataagg          >  1:955576‑M1/1‑20 (MQ=255)

                                                                                                                                   ttCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcg        >  2:779215‑M1/1‑18 (MQ=255)

                                                                                                                                      agagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgtatttgcaaacttttgtaggccggataaggcgttc     <  2:610646‑M1/150‑136 (MQ=255)

                                                                                                                                      agagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttc     <  2:634876‑M1/150‑136 (MQ=255)

                                                                                                                                      agagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttc     <  1:913026‑M1/150‑136 (MQ=255)

                                                                                                                                       gagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcg    >  1:601890‑M1/1‑14 (MQ=255)

                                                                                                                                        agGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgc   >  2:599530‑M1/1‑13 (MQ=255)

                                                                                                                                         ggTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgcg  >  1:720340‑M1/1‑12 (MQ=255)

TAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGAC  >  CP009273/4097336‑4097623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`