| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,147 | G→C | 33.4% | intergenic (‑81/+514) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,147 | 0 | G | C | 33.4% | 156.6 / 34.2 | 79 | intergenic (‑81/+514) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (32/20); new base C (24/3); total (56/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAA > CP009273/359998‑360291 | gACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCg > 1:1385733/1‑150 (MQ=255) aTCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGc < 1:652421/150‑1 (MQ=255) ttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGGGCTTCCCGCCACCCCCCCTGCCGtt < 2:1341334‑M1/51‑1 (MQ=255) agagGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGCCCTGCCCCCCACCCCCCccgccgct > 1:749980/1‑148 (MQ=255) tgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGtt > 1:123937‑M1/99‑149 (MQ=255) ctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCtt > 1:364253‑M1/95‑149 (MQ=255) ctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTc > 1:586247‑M1/95‑150 (MQ=255) ctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTc > 1:638127‑M1/95‑150 (MQ=255) ctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTc > 2:652464‑M1/95‑150 (MQ=255) cTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑Gc < 1:912865/150‑1 (MQ=255) ctcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑g > 1:647691‑M1/92‑148 (MQ=255) cACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTCGCCCCCCCCCCCCGCGCCGTTGCTTT‑GCCAc > 2:1384251/1‑150 (MQ=255) aggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCaa > 1:1458800‑M1/89‑148 (MQ=255) aggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAAc > 1:342117‑M1/89‑149 (MQ=255) aggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACg > 2:1155973‑M1/89‑150 (MQ=255) ggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGGGGCCTGCCCGCCCCCCCCCCGGCCCCTGCTTC‑GCaaccc < 2:452767‑M1/63‑3 (MQ=255) ccGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCTCCCGGTCGTTGTTTC‑GCaaaaa > 1:557591/1‑147 (MQ=38) cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGCCGTTGCTTC‑GCAACG‑tt > 1:371759/1‑150 (MQ=255) gCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGCCCTCCCCCCCCCCCCCCCCGCCGTTGCTTC‑CCCACA‑TTc > 2:662225/1‑150 (MQ=255) cTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCa < 1:1408618/150‑1 (MQ=255) gcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGGCCGGCCCGCCACCCCCCCGGGCGCTGCTTCCgcaacttcca < 2:1460566‑M1/67‑6 (MQ=255) gttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATc > 1:1006297‑M1/81‑150 (MQ=255) ccAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGGCTCCCCCCCCCCCCCCCGGCCGTTGGTTT‑GCTACG‑TTCAAATc > 2:1255826/1‑150 (MQ=38) cAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCACCCCCCCGGCCGGTGCCTT‑GCCACC‑CTCAAAAcc > 2:620579/1‑150 (MQ=255) gCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGc < 2:234010/150‑1 (MQ=255) cGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCCGCCCGTGCTTC‑GCAACG‑TTCAAAtaccct > 2:123937/1‑145 (MQ=38) ggCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGTTCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTc < 2:1243472/150‑1 (MQ=255) ggCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCCGCCGGTGCTTC‑GCCACG‑TTCAAAAcccccc > 2:1268764/1‑146 (MQ=37) cTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTccc < 1:1243307/150‑1 (MQ=255) cTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCGCCACCCCCCCGGCCGTTGCCTT‑GCAAAGTTTCAAAT‑CGCTccc > 1:182977/1‑150 (MQ=255) aGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTg < 2:1431589/150‑1 (MQ=255) ataaaacgaaaggcccagtctttcggctgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTc > 2:661800‑M1/62‑150 (MQ=255) ccaccaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCCCCGGGCCGGTGCTTT‑GCAAAG‑TTCAAAACCCCTCCCCGCGGGttttttct > 1:678153/1‑145 (MQ=25) aaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCt > 1:486606‑M1/59‑149 (MQ=255) aacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTAc > 2:1390650‑M1/58‑150 (MQ=255) caTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCACCCCCCCGGCCGTTGCTTC‑TCAACC‑ATTAAAAACGCCCCCCGCGGGGTTTTCctcctc > 2:963211/1‑150 (MQ=25) tCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAg < 2:12792/150‑1 (MQ=255) aaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAgg > 1:452767‑M1/53‑150 (MQ=255) aggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGga > 1:657387‑M1/52‑150 (MQ=255) gTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGgaga < 2:1385733/150‑1 (MQ=255) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGagag > 2:305937‑M1/49‑150 (MQ=255) aGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGGTGCTTC‑GCAACG‑TTCAAATCCGCTCCCCGCGGATTTTTTCTACTCAGGAGAGGGTTCa > 1:1021711/1‑150 (MQ=21) aGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCCCTTCTTT‑GCAACG‑GTCAAATCCCCCCCCCGCGGGGTTGTCCTCCCCCCGAGAGCGTTCa > 2:593412/1‑150 (MQ=21) tcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCa > 1:348976‑M1/40‑147 (MQ=255) cGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTC‑GCAACG‑TTCAAATCCCCTCCCCGCGGGTTTGTCCTACTCCGGGGAGCGTTTACCGa > 1:652464/1‑149 (MQ=18) gatgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTCCCCCCCCCCCCCCCGGCCGCTGCTTC‑GCAACC‑TTCAAAATCCCCCCCCGCGGGGTTTTTCTTCTCCGGAGAGGGtttcccccc > 1:701435/1‑142 (MQ=16) actgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGaca > 1:1212943‑M1/37‑150 (MQ=255) gatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCTCCCGGCCCCTGCTTT‑CCAACC‑TTCAAATCCCCCCCCCGCGGGGTTTTTCTTCTCCGGGGAGCGTTCACCCacaaa > 1:1320314/1‑149 (MQ=18) agcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaaca > 1:1002719‑M1/33‑150 (MQ=255) agcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaaca > 1:1192025‑M1/33‑150 (MQ=255) gcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaacaa > 1:1460566‑M1/32‑150 (MQ=255) ctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAacaaca > 1:593412‑M1/30‑150 (MQ=255) ctttcgttttatttgatgcctgggaaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAacaaca > 1:564983‑M1/30‑150 (MQ=255) cTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGa < 1:662225/150‑1 (MQ=255) cTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCTCCCCCCCCCCCCCCCGCCGCTGCTTT‑GCCACC‑TTCAAATCCCCCCCCCGCGGGTTTTTTCTTCTCCGGAGAGCGGTCACCCCCAAacaacaa > 2:1002719/1‑148 (MQ=12) cgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAt > 1:1315145‑M1/26‑149 (MQ=255) cgttttatttgatgccgggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGAt > 2:951800‑M1/26‑149 (MQ=255) aaaCATGAGAATTCCGTTCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaa < 2:93329/149‑1 (MQ=255) aaaCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCACCCCCCCGGCCGCTGCTTC‑GCAACG‑GTCAAAAACCCTCCCCGCGGGTTTGTTCTACTCCGGAGAGCGGTTCCCGACAAAAAAAAGAaaaa > 1:521223/1‑149 (MQ=17) tatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAAc > 2:521223‑M1/21‑149 (MQ=255) aCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:424310/150‑1 (MQ=255) atcagaATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCACCCCCCCGGCCGTTGCTTT‑GCAAAG‑TTTAAATCCGCCCCCCGCGGGTTTTTCCTACTCAGGAGAGCGGTCACCGCCAAACAACAGATAAAAAGa > 1:678987/4‑149 (MQ=14) aTGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCCCCCCCCCGGCCGTTGCTTC‑GCAACG‑TTCAAATCCCCCCCCCGCGGGTTTTTTCTACTCAGGAGAGCGTTCACCGACAAACAACAAATaaaaaca > 1:548176/1‑146 (MQ=14) aTGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCCTTGCTTT‑CCAACC‑TTCCAATCCCCCCCCCGCGGGGTTTTTCTTCTCCCGAGGGCGTTCTCCCACCAACAACAAATaaaaaca > 2:348976/1‑146 (MQ=9) agaATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg < 2:695369/149‑1 (MQ=255) gaATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCGCCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGc < 2:451056/150‑1 (MQ=255) ctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg > 1:1221516‑M1/11‑150 (MQ=255) gaattaaTTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCGGCCGTTGCGTC‑GCAAAG‑TTCAAAACCGCCCCCCGCGGGTTTTTTTTACTCACGAGAGCGGTTACCCACAAACAACAAATAAAAAAAAAAGCCCCATCttt > 2:782327/6‑150 (MQ=255) gaattaaTTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCACCCCCCCGGCCGTTGCTTC‑GCAACG‑TTCAAATCCCCCCCCCGCGGCTTTTTTCTTCTCCGGAGAGCGTTCACCGACAAACAACAAATAAAAAGAAAAACCCAGTCctt > 2:1329502/6‑150 (MQ=11) ccccAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCCCCCCGCCGTTGCTTT‑TCCACG‑GTCCAAACCGCCCCCCGCGGGTTTGTTCTTCTCAGGGGAGGGGTCCCCGACAAAAAAAAAAGAAAAAGAAAGGCCCCGCCTTTc > 2:497885/1‑150 (MQ=2) ccccAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTC‑GCAACC‑TTCAAATCCGCCCCCCGCGGGTTTTTCCTACTCCGGAGAGCGTTCACCCACCAAAAACAAATAAAAAGAAAAGCCCCGACTTTc > 1:951800/1‑150 (MQ=11) ccccAATTCCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCACCCCCCCGGCCCTTGCTTT‑GCCAAG‑TTCAAATCCCCCCCCCGCGGGTTTTTCCTTCTCAGGAGAGCGTTCCCCGCCAAACAACAGAAAAAAAGAAAAACCCAGTCtttt > 1:874230/1‑149 (MQ=2) aattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc > 1:455501‑M1/5‑150 (MQ=255) cAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 1:840040/150‑1 (MQ=33) cAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:804193/150‑1 (MQ=33) cAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 2:331995/150‑1 (MQ=33) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt < 2:875172/150‑1 (MQ=32) aTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTg < 1:874018/150‑1 (MQ=32) cGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat > 1:305937/1‑150 (MQ=33) gCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat > 2:295605/1‑149 (MQ=33) ccagcGGGCCGTTGATTC‑GCAACG‑TTCAAATCCGTTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattgggaa < 2:6209/146‑1 (MQ=255) | GACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTC‑GCAACG‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAA > CP009273/359998‑360291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |