Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,551,119 A→C 16.1% T80P (ACC→CCC)  osmC → lipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,551,1190AC16.1% 670.1 / 15.9 255T80P (ACC→CCC) osmClipoyl‑dependent Cys‑based peroxidase, hydroperoxide resistance; salt‑shock inducible membrane protein; peroxiredoxin
Reads supporting (aligned to +/- strand):  ref base A (113/89);  new base C (2/49);  total (115/140)
Fisher's exact test for biased strand distribution p-value = 5.70e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

ACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAG  >  CP009273/1550970‑1551268
                                                                                                                                                         |                                                                                                                                                     
ccagcgcccaatattttcgatttcaccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑Ta                                                                                                                                                       >  2:983358‑M1/32‑150 (MQ=255)
     cAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑Taccacc                                                                                                                                                  >  1:286762/1‑150 (MQ=255)
      aGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCAccg                                                                                                                                                 <  1:313694/150‑1 (MQ=255)
      aGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCAccg                                                                                                                                                 >  2:635087/1‑150 (MQ=255)
       ccaatattttcgatttcaccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCAccgc                                                                                                                                                <  1:495203‑M1/126‑1 (MQ=255)
        caatattttcgatttcaccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCAccgcc                                                                                                                                               >  2:703598‑M1/24‑150 (MQ=255)
         aatattttcgatttcaccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCAccgccg                                                                                                                                              >  1:1437316‑M1/23‑150 (MQ=255)
                 tttAACACGCGTTTTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCg                                                                                                                                      >  2:4818/1‑150 (MQ=255)
                    tttcaccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTg                                                                                                                                   <  2:1237738‑M1/139‑1 (MQ=255)
                     ttcaccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTg                                                                                                                                   <  2:1144596‑M1/139‑1 (MQ=255)
                       acGCGTTTGGAGGGC‑AAAAAAGAACCAACCCCTGAAAAACTGATGGGCGCGGCGCATGCCGCTTGTTTCTAAAGGGCGTTTTCATTAAGGCTGGGGGAGG‑GGGGATTCACGCAAACATCGT‑TTGA‑TACCACCGCCGATGTGTCGCTGGAt                                                                                                                                <  2:950788/150‑1 (MQ=255)
                        accaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGAt                                                                                                                                <  2:722164‑M1/142‑1 (MQ=255)
                        accaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGAt                                                                                                                                <  2:941239‑M1/142‑1 (MQ=255)
                         ccaatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATa                                                                                                                               <  2:161850‑M1/143‑1 (MQ=255)
                          caatcTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATa                                                                                                                               <  1:434616‑M1/143‑1 (MQ=255)
                          caatcTGAAGGCGAAAAAGAAACCAA‑CCCTGAAGAACTGATGGGCGCGGCGCATGCCGCATGTTTCTCAATGGCGTTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGT‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATaaa                                                                                                                             >  2:515113‑M1/6‑150 (MQ=255)
                             tttGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTg                                                                                                                          >  2:793116/1‑150 (MQ=255)
                             ttagAAGGGAAAAAAAAAACCAC‑CCGTGAAGATCTGAGTGGCGCAACACATGCGGCATGTTTCTAAAGGGCGCTTACATAAATGCTGGGGAAAG‑CGGATTTCACCCCAACACCGA‑TTGA‑AACCACCGCCGATGTGTCGCTGGATAAAGt                                                                                                                           <  1:177770/146‑1 (MQ=255)
                              ttGAAGGCAAAAAAGAACCCAC‑CCCTGAAGAACTGATGGGCGCAGCGAAGGCCGCTTTTTTCTAAATGGCGTTTTCATTAATGCTGGGGGAAG‑GGGGATTCACGCCAACATCGT‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTg                                                                                                                          <  2:775996/149‑1 (MQ=255)
                               tGAAGGAAAAAAAGAAACCAA‑CCAAAAAAAAGTGATGGGCGCGGCAAATGCAGCATGTTTCTAAATGGCGCTTTAATAAATGCTGGGGGAAG‑GGGGATCCACGCCAAAACCGA‑TTAA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGa                                                                                                                        <  2:1116508/150‑1 (MQ=255)
                                 agggCAAAAAAGGACCCAC‑CCCTGAAGAACTGATTGGCGCAGCGCAGGCCGCATTTTTCTAAATGGCGTTTTCATTAATGCTGGGGGAGG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGAt                                                                                                                       <  1:1116603/147‑1 (MQ=255)
                                 aaGGCAAAAAAGAAACCAC‑CCCTGAAAAACTGATTGGCGCGGCGCATCCCGCTTTTTTCTCAATGCCGCTTTCATTAATGCTGGGGAAGG‑CGGGATTCACGCCACCATCGT‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGAt                                                                                                                       <  1:825994/149‑1 (MQ=255)
                                  aGGCGAAAATGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCAGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACAGCCGATGTGTCGCTGGATAAAGGGGATGc                                                                                                                     >  2:39048/1‑150 (MQ=255)
                                   gccaaaaaaGAAACCAC‑CCCTGAAGACCTGTTCGGCCCAGCGCATGCCGCATTTTCCCCACTGCCGTTTTCATTAA‑CCTGGGGGGAGCCGGGGTCCACCCCAACACCAT‑TTGT‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGcc                                                                                                                    <  1:1359711/146‑1 (MQ=255)
                                    gCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCg                                                                                                                   <  2:1329258/150‑1 (MQ=255)
                                      gAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGt                                                                                                                 >  1:430659/1‑150 (MQ=255)
                                        aaagGGAACAAA‑CCCGGAAGGCCTGGTTGGCGCGGGGAGTGCCAAATATTTCTGGATGGCCTTTTCATAATGGCTGGGGGAAG‑GGAAATTCGCGACAACATCGA‑TTGA‑CGCCGCCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGttt                                                                                                               <  2:324132/150‑1 (MQ=255)
                                         aaaGGAACCAC‑CCTGGAAAAGCTGATGGGCGCCGCGCTTGCCGCAGGTTTCTGAAGGGCGCTTTCATTAATGTTGGGGGAAG‑GGGAATTCCCCCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGtttt                                                                                                              <  1:123479/150‑1 (MQ=255)
                                         aaaGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGtttt                                                                                                              >  2:1293071/1‑150 (MQ=255)
                                              aaccac‑ccCGGAAGGACTGTTTGGCGCGGCGCATGCCGCATGTTCCTAAATGGCGTTTTCATTAATGCTGGGGGAGG‑CGGGATTCAGGCCAACATCAA‑TTTA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGAt                                                                                                         <  2:751773/150‑1 (MQ=255)
                                               accaa‑ccCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGAt                                                                                                         >  2:265230/1‑149 (MQ=255)
                                               accaa‑ccCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGATTTGCGAt                                                                                                         >  1:544972/1‑149 (MQ=255)
                                                ccaa‑ccCAGAAGACCTGTTTGGCGCAGCCCATGCCGCGTTTTCCTCAATGGCGCTTTCATTAATGCTGGGGGAGG‑GGGGATTCCCGCCAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTa                                                                                                       <  1:260549/150‑1 (MQ=255)
                                                  caccccTGAAGAACTGATTGGCCCAGCGCAGGCCGCATGTTTCCCAATGCCGCTTTCATTAATGCTGGGGGAAG‑CGGGATCCACGCCACCCTCGA‑TTAA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTAc                                                                                                      <  1:1040973/147‑1 (MQ=255)
                                                  aaccccTAAAAAACGGATTGGCGCAGCGCATCCCGAATTTTTCTCAATGGCGTTTTCATAAAGGCTGGGGGAGG‑CGGGATTCACGCCAACATCGT‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTAc                                                                                                      <  2:539625/147‑1 (MQ=255)
                                                   a‑ccCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACg                                                                                                     >  1:265082/1‑149 (MQ=255)
                                                     cccTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGTTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGaa                                                                                                   <  1:354033/150‑1 (MQ=255)
                                                     cccTAAAGACCTGATGGGCGCGGCGCATGCCGCTTTTTTCTCAATGGCGTTTTCATTAATGTTGGGGGAGG‑CGGGATTCACGCCACCATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGaa                                                                                                   <  2:897652/150‑1 (MQ=255)
                                                     cccTAAAGAATTGATGGGCGCGGCGCATGCCGCATGTTTCCCAATGGCGTTTTCATTAATGCGGGGGGAGG‑CGGGATCCACGCCAACATGGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGaa                                                                                                   <  2:1359400/150‑1 (MQ=255)
                                                      ccTAAAGAACTGATGGGCGCAGCGCATGCCGAATGTTTCTCAAGGGCGTTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGaaa                                                                                                  <  1:1152398/150‑1 (MQ=255)
                                                       cTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGaaaa                                                                                                 >  1:88984/1‑150 (MQ=255)
                                                       cTGAAGAACTAATGGGCACAGCGCAGGCCGCATTTTCCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑GGGGATTCACGCCAACATCAA‑TTAA‑TCCCGCCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGaaaa                                                                                                 <  1:1278867/150‑1 (MQ=255)
                                                         gaagaaCTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATc                                                                                               >  1:553277/1‑150 (MQ=255)
                                                          aagaaCTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCg                                                                                              <  1:765121/150‑1 (MQ=255)
                                                          aagaaCTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCg                                                                                              >  1:748402/1‑150 (MQ=255)
                                                           agaaCTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCg                                                                                              >  2:405202/1‑149 (MQ=255)
                                                             aaCTGATGGGCGCAGCGCATGCCGCTTGTTTCTCAATGGCGTTTTCATTAATGCTGGGGGAGG‑CGGGATTCACGCAAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCAc                                                                                           <  2:1157610/150‑1 (MQ=255)
                                                               cTGATTGGCGCGGCGCATGCCGCATTTTTCTCAATGGCGCTTTAATTAATGCTGGGGGAGG‑CGGGATTCACGCCAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTg                                                                                         <  1:1305747/150‑1 (MQ=255)
                                                                tGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTg                                                                                         >  2:1398774/1‑149 (MQ=255)
                                                                tGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTg                                                                                         >  1:446925/1‑149 (MQ=255)
                                                                 gATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTg                                                                                         >  2:215908/1‑148 (MQ=255)
                                                                  aTTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAag                                                                                      >  1:1179664/1‑150 (MQ=255)
                                                                   ttGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGTTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAaga                                                                                     <  2:213827/150‑1 (MQ=255)
                                                                   ttGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGaa                                                                                       >  1:719256/1‑148 (MQ=255)
                                                                     ggCGCAGCGCATGCCGATTGTTTCTAAATGGCGTTTTCATTAATGCTGGGGGAGG‑CGGGATTCACGCAAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGt                                                                                   <  2:262015/150‑1 (MQ=255)
                                                                       cgcagcgcaTGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTg                                                                                  >  2:294297/1‑149 (MQ=255)
                                                                       cgcagcgcaTGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTg                                                                                  >  1:1332348/1‑149 (MQ=255)
                                                                          agcgcaTGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGt                                                                              >  1:1420724/1‑150 (MQ=255)
                                                                           gcgcaTGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGtt                                                                             <  1:635087/150‑1 (MQ=255)
                                                                             gcaTGCCGCATGTTTCTCAATGGCGTTTTCATTAATGCTGGGGGAAG‑CGGGTTCCACGCCAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGc                                                                           <  1:451602/150‑1 (MQ=255)
                                                                              taTGCCGATTTTTTCAAAATGGCGTTTTAATTAATGTTGGGGGAGG‑GGGTATTCACCCAAACATCGA‑TTGA‑CCCCTCCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCg                                                                          <  2:1438494/149‑1 (MQ=255)
                                                                                       tGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCggg                                                                  >  1:1353/1‑149 (MQ=255)
                                                                                         tttCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTAt                                                               >  1:1093995/1‑150 (MQ=255)
                                                                                          ttCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTAtt                                                              >  1:700927/1‑150 (MQ=255)
                                                                                           tctcAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTAtt                                                              <  1:722164/149‑1 (MQ=255)
                                                                                            ctcAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGa                                                            >  1:920486/1‑150 (MQ=255)
                                                                                            ctcAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGa                                                            >  2:601791/1‑150 (MQ=255)
                                                                                               aaTGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGa                                                            <  1:215908/147‑1 (MQ=255)
                                                                                                  ggCGCTTTCATTAATGCTGGGGGAGG‑CGGGATTCACACCAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCctc                                                      <  1:125654/150‑1 (MQ=255)
                                                                                                   gcgcTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCctct                                                     <  2:430659/150‑1 (MQ=255)
                                                                                                    ctcTTTCATTAAGGCTGGGGGAGG‑CGGGATTCACGCAAACATCGA‑TTGA‑TCCCCCCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTa                                                    <  1:329140/148‑1 (MQ=255)
                                                                                                    cgcTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTa                                                    >  2:579111/1‑150 (MQ=255)
                                                                                                     gcTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTAc                                                   >  1:1309033/1‑150 (MQ=255)
                                                                                                     gcTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTAc                                                   >  2:1078873/1‑150 (MQ=255)
                                                                                                     gcTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTAc                                                   >  2:683292/1‑150 (MQ=255)
                                                                                                         tCATTAATGCGGGGGGAGG‑CGGGATCCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCtt                                                <  2:1224789/149‑1 (MQ=255)
                                                                                                          cATTAATGCTGGGGGAAG‑GGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCttt                                               <  2:66991/149‑1 (MQ=255)
                                                                                                           aTTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTg                                              <  1:1387711/149‑1 (MQ=255)
                                                                                                              attggtGGGGGAGG‑GGGCATTCCCGCCAACA‑CGATTTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTTCCTTTGACg                                           <  2:131035/144‑1 (MQ=255)
                                                                                                               aTGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACgg                                          >  1:1215679/1‑149 (MQ=255)
                                                                                                                    ggggagaG‑CGGAATTCCCCCCAACAGCGT‑TTGT‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAAt                                    <  2:1250483/144‑1 (MQ=255)
                                                                                                                     ggggAAG‑CGGGTTTCACCCCAATATC‑A‑TTAAGAACCGCCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATc                                   <  1:572219/150‑1 (MQ=255)
                                                                                                                     ggggAAG‑CGGGATTCCCGCAAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATc                                   <  2:805451/150‑1 (MQ=255)
                                                                                                                         aaG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAg                                >  1:669234/1‑149 (MQ=255)
                                                                                                                           g‑CGGGATTCCCCCCAACATCGA‑TTGA‑TCCCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaa                             <  2:1418013/150‑1 (MQ=255)
                                                                                                                             cGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaag                            <  1:796435/150‑1 (MQ=255)
                                                                                                                              gggATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaag                            >  2:450915/1‑149 (MQ=255)
                                                                                                                                gATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaagca                          >  1:845535/1‑149 (MQ=255)
                                                                                                                                 aTTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaagcaaa                        <  2:131575/150‑1 (MQ=255)
                                                                                                                                 aTTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaagcaaa                        <  2:859420/150‑1 (MQ=255)
                                                                                                                                  ttCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGaaagcaa                         >  1:746647/1‑148 (MQ=255)
                                                                                                                                         cAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGAt                >  2:333744/1‑150 (MQ=255)
                                                                                                                                         cAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGCATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGAt                >  2:363771/1‑150 (MQ=255)
                                                                                                                                           aCATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGc              >  2:1032668/1‑150 (MQ=255)
                                                                                                                                            cATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc             >  1:993104/1‑150 (MQ=255)
                                                                                                                                            cATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc             >  1:384571/1‑150 (MQ=255)
                                                                                                                                            cATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGcc             >  1:1015682/1‑150 (MQ=255)
                                                                                                                                             aTCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGccc            >  2:234265/1‑150 (MQ=255)
                                                                                                                                               cGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCgg          >  1:1172507/1‑150 (MQ=255)
                                                                                                                                                        tACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCa   <  1:686527/150‑1 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
ACCAACAGCCGTATGGATTTAACACGCGTTTTGAAGGCGAAAAAGGAACCAA‑CCCTGAAGAACTGATTGGCGCAGCGCATGCCGCATGTTTCTCAATGGCGCTTTCATTAATGCTGGGGGAAG‑CGGGATTCACGCCAACATCGA‑TTGA‑TACCACCGCCGATGTGTCGCTGGATAAAGTGGATGCCGGTTTTGCGATTACGAAAATCGCACTGAAGAGTGAAGTTGCGGTGCCGGGTATTGATGCCTCTACCTTTGACGGCATAATCCAGAAAGCAAAAGCAGGATGCCCGGTCTCTCAG  >  CP009273/1550970‑1551268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑