Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,145,172	C→T	15.1%	intergenic (+45/+20)	yegJ → / ← yegK	uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,145,172	0	C	T	15.1%	109.2 / 4.3	63	intergenic (+45/+20)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base T (6/4);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGC  >  CP009273/2145025‑2145314                                                                                                                                                      |                                                                                                                                                taCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCt                                                                                                                                                <  2:625014/149‑1 (MQ=255)   cgcgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCa                                                                                                                                             <  1:479293/150‑1 (MQ=255)       cgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCg                                                                                                                                        <  1:90256/150‑1 (MQ=255)       cgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCg                                                                                                                                        <  2:774579/150‑1 (MQ=255)       cgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCg                                                                                                                                        <  2:527211/150‑1 (MQ=255)        ggggATAAAATTTACGGCGGTTATAACATTTATTCTTTTTTTGTGACCTATCCCAAAAAAAAAACCGACGAGCTAAAAACAAAAATAATTTGCTAATCAACCCTTATTTTTATTTTTTTAAATTGCGGGTTGAA‑TTTTTTTTTTTAA‑ccc                                                                                                                                        >  1:920480/1‑149 (MQ=255)                      cggcggTTATACCATTGATCCTTTATTTGTCACCTATCCAAAAGAAGAAGCCGGCGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGGTGGA‑TTTTTTTTTTTCA‑CCCCCCCGAGaaaaccac                                                                                                                         >  2:392087/1‑145 (MQ=255)                       ggcggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGGTTAA‑TTTTTGTTTTTAA‑ACGCCAAGAGAAaattact                                                                                                                        >  1:1309117/1‑150 (MQ=255)                          ggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTCAA‑CCGCCATGAGAAAATCACTCTg                                                                                                                     >  2:1310454/1‑150 (MQ=255)                          ggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTg                                                                                                                     >  2:213358/1‑150 (MQ=255)                              ataCCTTTGATCTTTTATTGGTCACCTATCAAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTAGTTCGCTAATCAGCCTTTATTTTTT‑TTGTTAAAACGGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGccc                                                                                                                  <  2:583524/149‑1 (MQ=255)                               taCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCCTGAGAAAATCACCCTTCCca                                                                                                                 >  1:55196/1‑149 (MQ=255)                                 cttttGATCTTTTATTGTTCCCCTATCCAAAGGAAAAACCCGGCGTGGTAAAAAAAAAATTTGTCCGCTATTCAGCGGTTTTTTTTA‑TTGTTTAAGCTGGGGTTTGAT‑TTGTTTTTTTCAG‑CCGCCATGAGAAACTCACTCTGCCcaca                                                                                                               <  1:1042841/146‑1 (MQ=255)                                   aTTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAgcg                                                                                                            >  1:1253508/1‑150 (MQ=255)                                             tATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTTTTTTCAA‑CCGCCTGGAGAAACTTAACCTGCCCCCAGCGCCAGTGCCaa                                                                                                  >  1:1076727/1‑150 (MQ=255)                                                    cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTa                                                                                           >  1:279689/1‑150 (MQ=255)                                                      ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑ACGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACCTTTTat                                                                                          >  2:1017081/1‑149 (MQ=255)                                                         aTCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcat                                                                                       >  1:745257/1‑149 (MQ=255)                                                            aaaaaaaAGAAGCCGACGAGATAAGAAAAAATTTATTTCGCAAAACAGCTTTTATATTTT‑TTTTTTAACCGGGGGGTTAAT‑TTTTTGTCTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcatc                                                                                   <  1:747189/149‑1 (MQ=255)                                                            aaaaaGAAGAAGCCGACGAGCTAAAAGAAAATTTAGTTCGCTAATCAGCCGTTATTTTTA‑TTGTTTAACCTGGCGGTTGAT‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat                                                                                    <  2:632055/148‑1 (MQ=255)                                                              aaaaaaGAAGCGGACGAGAAAAGAGAAAATTTTTTTCGCTAACCAGCCGTTATATTTT‑TTTTTAAACCGGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                 <  2:856477/150‑1 (MQ=255)                                                              aaaGAAGAAGCCGACGAGCAAAGAGAAAAATTATTTCGTTAACCAGCCGTTATTTTTA‑TTGTTAAAACTGGCGGTTGAT‑TTGTTGTCTTAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                 <  2:231977/150‑1 (MQ=255)                                                                  aagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTTAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGCTTTATCATCATCCGTACg                                                                             >  2:923811/1‑150 (MQ=255)                                                                  aaaaagCAGAGGAGCAAAGAAAAAAATTAGTTCGATAATCAGCCTTTATATTTA‑TTTTTAAAACTGGCGGTTGAA‑TTTTTGTCTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACg                                                                             <  1:840804/147‑1 (MQ=255)                                                                        ccGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGGA‑TTTTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCCGTGCCAACGGTTTTTCATCATTCGTACGCCCGtt                                                                       >  2:1132071/1‑150 (MQ=255)                                                                         cgacgaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTTAA‑TTGTTGTCTTTAA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCCGACGCTCGtt                                                                       >  1:498634/1‑149 (MQ=255)                                                                          gacgaGTAAAGAAAAAAATTAGTTCTATAATCAGCCTTTATTTTTA‑TTTTTTAACCTGGGGGTTAAT‑TTGTTTTTTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTa                                                                     <  2:55196/150‑1 (MQ=255)                                                                          gacgaGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTa                                                                     >  1:758524/1‑150 (MQ=255)                                                                                cTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTTTTTTTTTTAA‑CCGCCATGAGAAACTCACTCTTCCCACAGCGCCCGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTTccgccg                                                               >  2:1186550/1‑150 (MQ=255)                                                                                     agCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGGACGCCCGTTTACCGCCGGGCTg                                                          >  1:426891/1‑150 (MQ=255)                                                                                       cAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGGTGTTTTCAA‑CCGCCTAGTGAAACTCACACTCCCCACAGCGCCCGTGCCAACGTTTTTTCATCATCCGTACCCTTGTTTAACGCCGGGCTggg                                                        >  2:1283207/1‑149 (MQ=255)                                                                                        aaaaTTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTTTTGTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGa                                                        >  2:105456/1‑149 (MQ=255)                                                                                          aaTTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGGCAACGGTTTTTCATCATCCCTACGCTCGTTTAACCCCGGGCTGGGCa                                                      >  2:48204/1‑149 (MQ=255)                                                                                           attattCCGCTAATCAG‑CTTTTTTTTTT‑TTTTTTAAAGCGGGGGTTTAATTTGTGGTCTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaa                                                    <  2:498634/150‑1 (MQ=255)                                                                                             tAGTTCTCTAATCACCTTTTATTTTTT‑TTTTTAAACCTGGGGGTTAAT‑TTGTTGTCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa                                                   <  2:279689/149‑1 (MQ=255)                                                                                              aGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGGTGAA‑TTGTTGTCTTCAA‑ACGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGGCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaaa                                                  >  2:1005447/1‑149 (MQ=255)                                                                                               gTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTTTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACt                                                >  2:85759/1‑150 (MQ=255)                                                                                                tccGCTAACCCGCCTTTATTTTTT‑TTTTTTAAACGGGGGTTTAAT‑TTGTTTTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACt                                                <  2:1047235/147‑1 (MQ=255)                                                                                                  cccTAATCAGCGTTTATTTTTA‑TTTTTTAACCGGGCGTTTAAT‑TTTTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGc                                              <  1:858634/147‑1 (MQ=255)                                                                                                  cGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTTTCTTCAA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCCCGGTTACCGCCCGGCTGGACAAAAAATGCt                                             >  2:23621/1‑150 (MQ=255)                                                                                                       aTCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTaa                                         >  1:155144/1‑149 (MQ=255)                                                                                                        tCAGCCGTAATATTTA‑TTGTTAAAACGGGGGTTTGAA‑TTGTTTTCTCAAG‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACa                                       <  1:1366604/150‑1 (MQ=255)                                                                                                         cAGCCTTTATATTTT‑TTTTTAAACCTGGGGTTTAAT‑TTTTTTTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACaa                                      <  1:290263/150‑1 (MQ=255)                                                                                                          aGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTTAA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTTCCAACGTTTTATCATCATCCGTACGCTCGTTTATCGCCGGGCTGGACAAAAACTGCTTTAACAAt                                     >  1:141342/1‑150 (MQ=255)                                                                                                            cctttATATTTT‑TTGTTAAAACGGGGGGTTGAT‑TTTTTGTCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTc                                   <  1:87293/147‑1 (MQ=255)                                                                                                              gTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAAAAATTCAg                                 >  1:312477/1‑150 (MQ=255)                                                                                                                tataTTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTTAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAgg                                >  1:1190146/1‑149 (MQ=255)                                                                                                                   tttta‑ttGTTTAACCGGGGGTTTGAT‑TTGTTTTCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaa                             <  1:1310454/148‑1 (MQ=255)                                                                                                                   attta‑ttGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaa                             >  1:72891/1‑149 (MQ=255)                                                                                                                    ttta‑ttGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTTAA‑CCGCCATGAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaat                            >  1:402869/1‑149 (MQ=255)                                                                                                                     tta‑ttGTTTAAACTGGCGGTTTAA‑TTGTTGTCTTCAA‑CCGCCCTGAGAAAATCACCCTGCCCACAGCGCCAGTGCCCACGTTTTTTCATCATTCGTACGCTCGTTTACCGCCCGGCTGGACAAAAACTGCTTTTACAATTCAAGgaata                           >  2:1462760/1‑149 (MQ=255)                                                                                                                       a‑ttTTTTAAACTGGCGGTTAAA‑TTGTTTTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGAAATaaa                         <  2:690846/149‑1 (MQ=255)                                                                                                                         ttGTTAAAACGGGGGTTTAAA‑TTTTGGTCTAGAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc                       <  2:811104/150‑1 (MQ=255)                                                                                                                          ttttaaaaCTGGCGTTTAAT‑TTTTTTTCTCCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc                       <  2:1061222/144‑1 (MQ=255)                                                                                                                                aaCTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttgt                >  2:381123/1‑150 (MQ=255)                                                                                                                                     ggcgTTTAA‑TTGTTTTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCt            <  2:426891/147‑1 (MQ=255)                                                                                                                                      ggtttGAT‑TTTTTGTTTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGc          <  1:797949/147‑1 (MQ=255)                                                                                                                                      cGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGc          >  1:1126348/1‑150 (MQ=255)                                                                                                                                       ggTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg         >  1:1119909/1‑150 (MQ=255)                                                                                                                                         ttGAA‑TTGTTTTCTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGtt       <  2:23557/150‑1 (MQ=255)                                                                                                                                          tGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtg      >  1:211138/1‑150 (MQ=255)                                                                                                                                             attttttgtCTTAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct    <  2:865662/145‑1 (MQ=255)                                                                                                                                               ttgttaTCTACAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgctgc  <  1:1217865/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                TACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTA‑TTGTTTAAACTGGCGGTTGAA‑TTGTTGTCTTCAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGC  >  CP009273/2145025‑2145314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑