Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,486 G→C 61.2% L83L (CTG→CTC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,4860GC61.2% 174.4 / 256.7 183L83L (CTG→CTC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base G (30/41);  new base C (50/62);  total (80/103)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-02

Only 100 of 183 total aligned reads displayed.

ACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCA  >  CP009273/8341‑8631
                                                                                                                                                 |                                                                                                                                                 
accCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGaaa                                                                                                                                               >  2:905427/1‑150 (MQ=255)
     tctctcATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCt                                                                                                                                           >  2:564471/1‑149 (MQ=255)
              ctcggcgcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGGTGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTgat                                                                                                                                  <  1:545652/144‑3 (MQ=255)
              ctcggcgcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTgat                                                                                                                                  <  2:1186683/144‑3 (MQ=255)
                 ggcgcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCg                                                                                                                              <  1:1139197/148‑1 (MQ=255)
                 ggcgcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCg                                                                                                                              <  1:383450/148‑1 (MQ=255)
                  aCGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCgg                                                                                                                             <  2:1199968/150‑1 (MQ=255)
                    gcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGc                                                                                                                           >  1:328867/7‑150 (MQ=255)
                        ctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTa                                                                                                                       >  2:1389790/3‑150 (MQ=255)
                        ctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTa                                                                                                                       >  1:742041/3‑150 (MQ=255)
                        ctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTa                                                                                                                       >  2:386149/3‑150 (MQ=255)
                        cgcagATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTa                                                                                                                       >  2:966603/1‑150 (MQ=255)
                        cgcagATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTa                                                                                                                       >  2:263741/1‑150 (MQ=255)
                         gcagATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTAt                                                                                                                      >  2:150514/1‑150 (MQ=255)
                            gATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTAtctc                                                                                                                   >  1:637988/1‑150 (MQ=255)
                                cctgagtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCAcc                                                                                                               >  2:16366/7‑149 (MQ=255)
                                 ctgagtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCAccg                                                                                                              <  1:32753/145‑3 (MQ=255)
                                 cGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACt                                                                                                               <  2:806057/149‑1 (MQ=255)
                                    agtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAg                                                                                                           >  2:613413/3‑150 (MQ=255)
                                    aaTACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAg                                                                                                           >  1:102506/1‑150 (MQ=255)
                                        ccGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGa                                                                                                       >  2:1231274/1‑150 (MQ=255)
                                         cGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGAt                                                                                                      <  1:889581/150‑1 (MQ=255)
                                          gTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGt                                                                                                          <  1:220808/145‑1 (MQ=255)
                                           tAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgc                                                                                                    <  2:77019/150‑1 (MQ=255)
                                             agctgattgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgcac                                                                                                  <  2:1170958/147‑3 (MQ=255)
                                             agctgattgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgcac                                                                                                  <  1:819991/147‑3 (MQ=255)
                                             agctgattgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgcac                                                                                                  <  2:474069/147‑3 (MQ=255)
                                              gTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGcgcg                                                                                                 >  1:514358/1‑150 (MQ=255)
                                              gTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGcgcg                                                                                                 <  2:102506/150‑1 (MQ=255)
                                                 gattgatGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTc                                                                                               <  1:358440/149‑1 (MQ=255)
                                                    tgatgaTGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGa                                                                                                       >  1:165945/1‑138 (MQ=255)
                                                      atgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTcc                                                                                          >  1:612297/1‑149 (MQ=255)
                                                         atgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTAt                                                                                       <  2:954018/149‑1 (MQ=255)
                                                         atgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATg                                                                                      <  2:469130/150‑1 (MQ=255)
                                                                    tGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCg                                                                           >  1:395229/1‑150 (MQ=255)
                                                                      ggCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGt                                                                          <  2:1412350/149‑1 (MQ=255)
                                                                          aaaCAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAAtt                                                                     >  2:1419802/1‑150 (MQ=255)
                                                                           gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTAt                                                                                       <  1:1441606/126‑1 (MQ=255)
                                                                           gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAtc                                                                     <  1:165662/144‑2 (MQ=255)
                                                                             cagcagAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCg                                                                  <  1:150514/150‑1 (MQ=255)
                                                                             agccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAtc                                                                     <  1:1178337/144‑2 (MQ=255)
                                                                             agccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGc                                                                   >  1:1397927/4‑149 (MQ=255)
                                                                             agccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGc                                                                   >  2:368954/4‑149 (MQ=255)
                                                                              gccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATcgcg                                                                  <  2:528047/147‑1 (MQ=255)
                                                                               gcagAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGaa                                                                <  2:876283/150‑1 (MQ=255)
                                                                                 agagCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAACTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAg                                                              >  1:700620/1‑150 (MQ=255)
                                                                                   agCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCa                                                            <  1:444012/150‑1 (MQ=255)
                                                                                     cagcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGcc                                                            >  1:1113995/4‑147 (MQ=255)
                                                                                      aaCGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                                         <  1:841537/150‑1 (MQ=255)
                                                                                      aaCGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCaaaa                                                         <  1:263741/150‑1 (MQ=255)
                                                                                         gATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGc                                                      <  2:514358/150‑1 (MQ=255)
                                                                                         gATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCg                                                       >  1:61949/1‑149 (MQ=255)
                                                                                          aTCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgca                                                     <  2:698419/150‑2 (MQ=255)
                                                                                            cgcgcgCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                                    >  2:1101171/1‑149 (MQ=255)
                                                                                             gcgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                                  >  2:217033/1‑150 (MQ=255)
                                                                                               gcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                                <  1:1426316/150‑1 (MQ=255)
                                                                                                cgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                               <  1:1362153/150‑1 (MQ=255)
                                                                                                 gcagcaGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCa                                               >  2:860577/1‑149 (MQ=255)
                                                                                                 gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa                                              <  2:1204336/150‑1 (MQ=255)
                                                                                                 gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa                                              <  2:1039831/150‑1 (MQ=255)
                                                                                                  cagcagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaag                                             <  2:827299/150‑2 (MQ=255)
                                                                                                     cagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGctg                                          <  2:732039/150‑2 (MQ=255)
                                                                                                      agATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActct                                         <  1:966603/150‑1 (MQ=255)
                                                                                                       gATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTa                                        <  2:745684/150‑1 (MQ=255)
                                                                                                        aTCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                                       <  2:661600/150‑1 (MQ=255)
                                                                                                              gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa                                  <  2:612297/149‑1 (MQ=255)
                                                                                                               aCGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATg                                <  1:1419689/150‑1 (MQ=255)
                                                                                                                 gcttccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGcg                              >  1:853012/5‑149 (MQ=255)
                                                                                                                  cttccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGcgg                             <  2:292133/147‑3 (MQ=255)
                                                                                                                  cttccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGcg                              <  1:482029/146‑2 (MQ=255)
                                                                                                                  cttccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGcg                              <  2:1348252/146‑2 (MQ=255)
                                                                                                                    tccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                                                  >  2:1368190/2‑123 (MQ=255)
                                                                                                                     ccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCgggca                          >  2:354387/1‑148 (MQ=255)
                                                                                                                     ccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCgggca                          >  1:120821/1‑148 (MQ=255)
                                                                                                                     ccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTa                          <  2:604308/150‑1 (MQ=255)
                                                                                                                      cgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCAt                         <  2:473502/150‑1 (MQ=255)
                                                                                                                      cgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCAt                         <  2:1324877/150‑1 (MQ=255)
                                                                                                                         taagcTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAg                      >  1:99154/5‑150 (MQ=255)
                                                                                                                           agcTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGc                     <  2:1224658/147‑1 (MQ=255)
                                                                                                                             cTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAAc                  <  1:1315543/150‑1 (MQ=255)
                                                                                                                             cTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAAc                  <  1:16366/150‑1 (MQ=255)
                                                                                                                             cTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAAc                  <  2:1067949/150‑1 (MQ=255)
                                                                                                                                 cAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATc              <  2:395229/150‑1 (MQ=255)
                                                                                                                                   gTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGt            <  1:564471/150‑1 (MQ=255)
                                                                                                                                     gaacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAt          >  1:36662/8‑150 (MQ=255)
                                                                                                                                     gaacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAt          >  2:332619/8‑150 (MQ=255)
                                                                                                                                     aaaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTAt          >  1:453002/1‑150 (MQ=255)
                                                                                                                                      aacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAt          <  1:1455466/143‑1 (MQ=255)
                                                                                                                                      aacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAt          <  1:190111/143‑1 (MQ=255)
                                                                                                                                      aaTATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTAtt         >  2:1429382/1‑150 (MQ=255)
                                                                                                                                        catcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCt       >  1:1104811/5‑150 (MQ=255)
                                                                                                                                         atcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCt       >  1:1187746/4‑149 (MQ=255)
                                                                                                                                         atcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTg      <  2:61949/147‑1 (MQ=255)
                                                                                                                                            ggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGAt    >  1:435668/1‑149 (MQ=255)
                                                                                                                                            ggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGAt    >  2:1034024/1‑149 (MQ=255)
                                                                                                                                            ggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGAt    >  2:939572/1‑149 (MQ=255)
                                                                                                                                             gTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATc   >  1:1041297/1‑149 (MQ=255)
                                                                                                                                             gTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCa  <  2:1269592/150‑1 (MQ=255)
                                                                                                                                             gTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATc   >  2:780742/1‑149 (MQ=255)
                                                                                                                                             gTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCa  <  2:856499/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCA  >  CP009273/8341‑8631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑