BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	8,282	G→C	35.8%	V15V (GTG→GTC)	talB →	transaldolase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	8,282	0	G	C	35.8%	139.1 / 153.6	120	V15V (GTG→GTC)	talB	transaldolase B
Reads supporting (aligned to +/- strand): ref base G (32/45); new base C (30/13); total (62/58)
Fisher's exact test for biased strand distribution p-value = 4.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.22e-01

Only 100 of 120 total aligned reads displayed.

GCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACG  >  CP009273/8135‑8430

gCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGc                                                                                                                                                    <  2:38825/150‑1 (MQ=255)

 cGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGcc                                                                                                                                                   <  1:385261/150‑1 (MQ=255)

    tAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGac                                                                                                                                                <  2:1038949/150‑1 (MQ=255)

       ccGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACt                                                                                                                                             <  1:341503/150‑1 (MQ=255)

          tCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTggg                                                                                                                                          >  2:1211255/1‑150 (MQ=255)

            ttGTCGGCGGTTTCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGGTACATCCCCCTAACAAGCTGTTTTAAGAGAAATACTATCATGACGGGCAAAATGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTgggg                                                                                                                                         >  2:23439/1‑149 (MQ=255)

              gTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGa                                                                                                                                        <  2:1375857/148‑1 (MQ=255)

                    ggTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCgg                                                                                                                                >  1:394934/1‑150 (MQ=255)

                     gTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGc                                                                                                                               <  2:1302493/150‑1 (MQ=255)

                      ttGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCa                                                                                                                              <  2:653679/150‑1 (MQ=255)

                         cgcTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACAGCGCGGCAAt                                                                                                                            <  2:907832/149‑1 (MQ=255)

                           cTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGaa                                                                                                                         <  2:1270158/150‑1 (MQ=255)

                                gTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGt                                                                                                                    <  1:471923/150‑1 (MQ=255)

                                gTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGt                                                                                                                    <  2:394934/150‑1 (MQ=255)

                                     gtcgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCaa                                                                                                               >  2:946803/1‑150 (MQ=255)

                                      tcgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAAc                                                                                                              <  2:1219164/150‑1 (MQ=255)

                                      tcgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAAc                                                                                                              <  2:1219144/150‑1 (MQ=255)

                                          gATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCg                                                                                                            >  1:1338879/1‑148 (MQ=255)

                                              tcatcaGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGa                                                                                                       <  1:1211255/149‑1 (MQ=255)

                                              tcatcaGGGCAGACCGGTTACACCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGa                                                                                                       <  2:1402842/149‑1 (MQ=255)

                                                  caGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCca                                                                                                  >  1:161569/1‑150 (MQ=255)

                                                   aGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCca                                                                                                  >  2:1365843/1‑149 (MQ=255)

                                                   aGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCca                                                                                                  >  1:1062724/1‑149 (MQ=255)

                                                         gACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaaccaa                                                                                           <  1:889147/150‑1 (MQ=255)

                                                         gACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaaccaa                                                                                           <  1:526171/150‑1 (MQ=255)

                                                         gACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaaccaa                                                                                           <  2:1131890/150‑1 (MQ=255)

                                                           ccGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACaaccaacc                                                                                         <  1:191258/150‑1 (MQ=255)

                                                            cGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAAccc                                                                                        <  1:1376037/150‑1 (MQ=255)

                                                             ggTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCt                                                                                       >  2:840047/1‑150 (MQ=255)

                                                             ggTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCt                                                                                       >  1:906211/1‑150 (MQ=255)

                                                                    tCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCa                                                                                <  1:1182634/150‑1 (MQ=255)

                                                                         cTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCtt                                                                           <  1:519572/150‑1 (MQ=255)

                                                                           aaCAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTaa                                                                         >  1:354067/1‑150 (MQ=255)

                                                                             cAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCtttaag                                                                       <  2:1062724/150‑5 (MQ=255)

                                                                                gCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcag                                                                    <  1:1316224/150‑1 (MQ=255)

                                                                                  tGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcg                                                                  >  1:909599/1‑150 (MQ=255)

                                                                                   gTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTTAcgcagcg                                                                  >  1:797988/1‑149 (MQ=255)

                                                                                       aaaGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGa                                                              <  1:705708/149‑1 (MQ=255)

                                                                                        aaGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGAtt                                                            >  2:840327/1‑150 (MQ=255)

                                                                                        aaGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGAt                                                             <  1:863475/149‑1 (MQ=255)

                                                                                                acacaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTa                                                    <  2:399656/143‑1 (MQ=255)

                                                                                                  cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCGCCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATAcc                                                  <  1:1387125/150‑1 (MQ=255)

                                                                                                  acaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTAcc                                                  >  1:558041/6‑150 (MQ=255)

                                                                                                   caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                                                 >  2:1070120/5‑150 (MQ=255)

                                                                                                   caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                                                 >  2:329329/5‑150 (MQ=255)

                                                                                                    aTCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCg                                                 >  1:820221/1‑149 (MQ=255)

                                                                                                      aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                                                >  2:1004355/2‑148 (MQ=255)

                                                                                                      aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGACGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCGCGGCGCGGCTCAGATCCCTGAGTACCgg                                                >  2:1226429/2‑147 (MQ=255)

                                                                                                       aTGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTaa                                              <  2:615812/149‑1 (MQ=255)

                                                                                                          aCGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAgct                                           >  1:571191/1‑147 (MQ=255)

                                                                                                           cGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctga                                         >  1:1229291/1‑150 (MQ=255)

                                                                                                            ggATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgat                                        <  2:628261/150‑1 (MQ=255)

                                                                                                            ggATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgat                                        <  1:485112/150‑1 (MQ=255)

                                                                                                            ggACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgat                                        >  2:579869/1‑150 (MQ=255)

                                                                                                              ataaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgatt                                       >  2:703276/3‑149 (MQ=255)

                                                                                                              ataaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgatt                                       >  2:168984/3‑149 (MQ=255)

                                                                                                              ataaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgatt                                       >  1:327012/3‑149 (MQ=255)

                                                                                                              ataaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgatt                                       >  1:1230926/3‑149 (MQ=255)

                                                                                                               taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga                                     >  2:177236/2‑150 (MQ=255)

                                                                                                                aaaTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTtgattgat                                    <  2:113968/150‑1 (MQ=255)

                                                                                                                aaaTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTtgattgat                                    >  1:1412350/1‑150 (MQ=255)

                                                                                                                aaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCt                                                                            >  2:308250/1‑110 (MQ=255)

                                                                                                                 aaTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATtgatg                                   <  1:23439/150‑1 (MQ=255)

                                                                                                                 aaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattgac                                    <  1:726299/149‑2 (MQ=255)

                                                                                                                 aaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattgac                                    <  1:381967/149‑2 (MQ=255)

                                                                                                                  aTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATtgatga                                  <  1:299508/150‑1 (MQ=255)

                                                                                                                  aTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATtgacga                                  >  1:541653/1‑150 (MQ=255)

                                                                                                                    tGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGatgatg                                >  2:1438521/1‑150 (MQ=255)

                                                                                                                      aCCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCt                              >  2:891334/1‑150 (MQ=255)

                                                                                                                        cTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgat                                        <  2:442693/138‑1 (MQ=255)

                                                                                                                          ctctgcGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGt                            <  1:1461454/143‑1 (MQ=255)

                                                                                                                           tctgcGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGc                         >  1:1339682/5‑150 (MQ=255)

                                                                                                                           tctgcGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGc                         >  1:473502/5‑150 (MQ=255)

                                                                                                                           ccTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGc                         >  1:1175435/1‑150 (MQ=255)

                                                                                                                             tgcGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGc                         <  2:70694/146‑1 (MQ=255)

                                                                                                                              gcGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTg                      >  1:698419/2‑150 (MQ=255)

                                                                                                                               cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                     >  2:217201/1‑150 (MQ=255)

                                                                                                                               cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                     >  2:276375/1‑150 (MQ=255)

                                                                                                                               cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                     >  1:451465/1‑150 (MQ=255)

                                                                                                                               cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                     >  1:762484/1‑150 (MQ=255)

                                                                                                                               cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                     <  1:794785/150‑1 (MQ=255)

                                                                                                                               cGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTgg                     >  1:845259/1‑150 (MQ=255)

                                                                                                                               cGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCt                       <  2:354067/148‑1 (MQ=255)

                                                                                                                               cGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTgg                     >  1:604308/1‑150 (MQ=255)

                                                                                                                                gTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTggg                    <  1:19236/150‑1 (MQ=255)

                                                                                                                                 tCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGc                   <  1:66381/150‑1 (MQ=255)

                                                                                                                                  cAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGct                  >  2:457260/1‑149 (MQ=255)

                                                                                                                                   aGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctc                 >  2:827683/1‑148 (MQ=255)

                                                                                                                                   aGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctc                 <  2:1111522/150‑3 (MQ=255)

                                                                                                                                     tACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGaaa               <  2:1411418/150‑1 (MQ=255)

                                                                                                                                      acacCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcg                >  1:1170958/1‑145 (MQ=255)

                                                                                                                                       caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag             <  2:1417477/150‑7 (MQ=255)

                                                                                                                                       caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag             >  2:931987/1‑144 (MQ=255)

                                                                                                                                       caccacTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGctcgtag             <  1:775895/150‑7 (MQ=255)

                                                                                                                                         ccaccGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcagc           <  2:1147268/150‑1 (MQ=255)

                                                                                                                                          caccGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcag            >  2:327947/1‑148 (MQ=255)

                                                                                                                                           accGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAAcag            >  2:541174/1‑147 (MQ=255)

                                                                                                                                             cGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCagag       >  1:806057/1‑150 (MQ=255)

                                                                                                                                                 gTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAAc   >  1:943104/1‑150 (MQ=255)

                                                                                                                                                 gTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAAc   <  2:9225/150‑1 (MQ=255)

GCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACG  >  CP009273/8135‑8430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`