BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,480	Δ1 bp	100%	coding (1/963 nt)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,480	0	A	.	100.0%	499.6 / NA	103	coding (1/963 nt)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (54/49); total (54/49)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 103 total aligned reads displayed.

TAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACC  >  CP009273/4097336‑4097624

taAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTt                                                                                                                                            <  2:91148‑M1/150‑2 (MQ=255)

taAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATT                                                                                                                                             <  1:72634‑M1/149‑1 (MQ=255)

 aaaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtc                                                                                                                                           >  1:595424‑M1/1‑148 (MQ=255)

  aaaTTCCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcg                                                                                                                                          <  2:890530‑M1/150‑4 (MQ=255)

  aaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtc                                                                                                                                           <  2:15419‑M1/149‑3 (MQ=255)

  aaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtc                                                                                                                                           <  2:793582‑M1/149‑3 (MQ=255)

          ccATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                  <  2:487648‑M1/150‑12 (MQ=255)

          ccATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                  >  2:477875‑M1/1‑139 (MQ=255)

           cATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                  >  1:981179‑M1/1‑138 (MQ=255)

             tttGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                  >  2:875762‑M1/1‑136 (MQ=255)

                ggCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagatt                                                                                                                            >  2:70080‑M1/1‑133 (MQ=255)

                ggCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagat                                                                                                                             <  1:391644‑M1/149‑17 (MQ=255)

                  cctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcc                                                                                                                          >  1:479239‑M1/1‑131 (MQ=255)

                   ctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcct                                                                                                                         <  2:225476‑M1/150‑21 (MQ=255)

                          gaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                  >  1:636999‑M1/1‑123 (MQ=255)

                           aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                  <  1:544950‑M1/149‑28 (MQ=255)

                              cAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaa                                                                                                              >  1:920530‑M1/1‑119 (MQ=255)

                               aaTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaac                                                                                                             >  2:894836‑M1/1‑118 (MQ=255)

                                aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                            <  2:433631‑M1/150‑34 (MQ=255)

                                  tCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccga                                                                                                          >  2:368254‑M1/1‑115 (MQ=255)

                                   cagcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                         >  1:506298‑M1/1‑114 (MQ=255)

                                      cagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                       >  2:499620‑M1/1‑111 (MQ=255)

                                       agGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                       >  2:54826‑M1/1‑110 (MQ=255)

                                        ggAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacag                                                                                                    <  1:570065‑M1/150‑42 (MQ=255)

                                           aGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                                 >  1:531414‑M1/1‑106 (MQ=255)

                                           aGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                                 <  1:531614‑M1/150‑45 (MQ=255)

                                            gTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                                 >  2:938124‑M1/1‑105 (MQ=255)

                                               aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaa                                                                                              <  2:370741‑M1/149‑48 (MQ=255)

                                               aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcagaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaa                                                                                             <  2:802818‑M1/150‑49 (MQ=255)

                                                   ttATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatg                                                                                         >  1:342944‑M1/1‑98 (MQ=255)

                                                     ataCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcc                                                                                       >  1:742562‑M1/1‑96 (MQ=255)

                                                     ataCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcc                                                                                       >  1:596098‑M1/1‑96 (MQ=255)

                                                      taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcct                                                                                      <  1:447381‑M1/150‑56 (MQ=255)

                                                      taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcct                                                                                      <  2:742562‑M1/150‑56 (MQ=255)

                                                      taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcc                                                                                       <  2:662313‑M1/149‑55 (MQ=255)

                                                         tATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgat                                                                                   >  2:157696‑M1/1‑92 (MQ=255)

                                                           tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgat                                                                                   <  2:833411‑M1/148‑59 (MQ=255)

                                                           tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgat                                                                                   <  2:625624‑M1/148‑59 (MQ=255)

                                                            ttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcg                                                                                >  2:414401‑M1/1‑89 (MQ=255)

                                                             tGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgc                                                                               >  2:973793‑M1/1‑88 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                             >  2:230874‑M1/1‑86 (MQ=255)

                                                                acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttc                                                                            <  1:129993‑M1/150‑66 (MQ=255)

                                                                 caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcg                                                                           >  1:163883‑M1/1‑84 (MQ=255)

                                                                 caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcg                                                                           >  1:622881‑M1/1‑84 (MQ=255)

                                                                   ttCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgct                                                                         <  1:188842‑M1/150‑69 (MQ=255)

                                                                    tCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgct                                                                         >  2:33804‑M1/1‑81 (MQ=255)

                                                                       ttGGATCACTTCGATGTGCAAGAAGACTTCTGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatc                                                                     <  2:119647‑M1/150‑73 (MQ=255)

                                                                        tGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttat                                                                      >  1:11796‑M1/1‑77 (MQ=255)

                                                                          gATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcagg                                                                  >  1:571762‑M1/1‑75 (MQ=255)

                                                                          gATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcag                                                                   <  2:674720‑M1/149‑75 (MQ=255)

                                                                           aTCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggc                                                                 >  1:500008‑M1/1‑74 (MQ=255)

                                                                           aTCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggc                                                                 <  2:596098‑M1/150‑77 (MQ=255)

                                                                             cACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcc                                                                >  2:256694‑M1/1‑72 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcct                                                               <  1:894836‑M1/149‑79 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcct                                                               <  1:230874‑M1/149‑79 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcct                                                               <  1:112930‑M1/149‑79 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagactcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggccta                                                              <  2:981179‑M1/150‑80 (MQ=255)

                                                                                ttCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctaca                                                            >  1:761045‑M1/1‑69 (MQ=255)

                                                                                  cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctaca                                                            <  1:85181‑M1/148‑82 (MQ=255)

                                                                                   gATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatg                                                          >  2:359954‑M1/1‑66 (MQ=255)

                                                                                     tgtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaat                                                       <  2:595424‑M1/150‑87 (MQ=255)

                                                                                      gtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaatt                                                      >  1:307760‑M1/1‑63 (MQ=255)

                                                                                         cAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                   <  1:271168‑M1/150‑91 (MQ=255)

                                                                                         cAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                   <  1:229007‑M1/150‑91 (MQ=255)

                                                                                          aagaagACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgc                                                  <  2:219165‑M1/150‑92 (MQ=255)

                                                                                           agaagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgc                                                  >  2:992021‑M1/1‑58 (MQ=255)

                                                                                            gaagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaa                                                >  1:818286‑M1/1‑57 (MQ=255)

                                                                                            gaagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaa                                                >  2:682389‑M1/1‑57 (MQ=255)

                                                                                             aagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaat                                               >  1:805115‑M1/1‑56 (MQ=255)

                                                                                              agaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatt                                              <  1:849633‑M1/150‑96 (MQ=255)

                                                                                               gaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatt                                              >  2:642830‑M1/1‑54 (MQ=255)

                                                                                               gaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatt                                              >  2:298488‑M1/1‑54 (MQ=255)

                                                                                                     cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaa                                       >  1:97907‑M1/1‑48 (MQ=255)

                                                                                                     cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaa                                       >  2:200834‑M1/1‑48 (MQ=255)

                                                                                                     cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattga                                        <  1:203368‑M1/149‑102 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                      >  1:144092‑M1/1‑47 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                      <  1:252782‑M1/150‑104 (MQ=255)

                                                                                                         aaCAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttg                                   >  2:134178‑M1/1‑44 (MQ=255)

                                                                                                            aGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcatacatgaattctgcaatttattgaatttgcaa                                <  1:774259‑M1/150‑110 (MQ=255)

                                                                                                              aTTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaac                              >  1:387410‑M1/1‑39 (MQ=255)

                                                                                                              aTTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaac                              >  2:953003‑M1/1‑39 (MQ=255)

                                                                                                               tttCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaact                             >  2:639718‑M1/1‑38 (MQ=255)

                                                                                                               tttCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaact                             <  1:157696‑M1/150‑113 (MQ=255)

                                                                                                                 tCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactt                            <  2:479239‑M1/149‑114 (MQ=255)

                                                                                                                   aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactttt                          <  2:622881‑M1/149‑116 (MQ=255)

                                                                                                                        gCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggc                    >  1:664025‑M1/1‑29 (MQ=255)

                                                                                                                        gCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtagg                     <  2:500008‑M1/149‑121 (MQ=255)

                                                                                                                          aTTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccg                  >  2:225096‑M1/1‑27 (MQ=255)

                                                                                                                           ttCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgtaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcagtcctacatgaattctgcatttttttgtttttgcaaatttttgtaggccgt                 >  1:775569‑M1/1‑26 (MQ=255)

                                                                                                                             ccAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccgga                <  1:477875‑M1/149‑126 (MQ=255)

                                                                                                                                aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag            >  2:325374‑M1/1‑21 (MQ=255)

                                                                                                                                  gTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggc          <  1:135873‑M1/150‑132 (MQ=255)

                                                                                                                                   ttCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcg         <  1:33804‑M1/150‑133 (MQ=255)

                                                                                                                                      aggggtagTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttc      <  1:150871‑M1/147‑136 (MQ=255)

                                                                                                                                      agagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttc      <  2:342944‑M1/150‑136 (MQ=255)

                                                                                                                                      agagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgtt       <  1:54826‑M1/149‑135 (MQ=255)

                                                                                                                                       gagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcg     >  1:586856‑M1/1‑14 (MQ=255)

                                                                                                                                        agGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgc    >  2:444648‑M1/1‑13 (MQ=255)

                                                                                                                                         ggTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgcg   >  2:920629‑M1/1‑12 (MQ=255)

                                                                                                                                          gTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgcgc  >  1:244950‑M1/1‑11 (MQ=255)

TAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGACC  >  CP009273/4097336‑4097624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`