Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC CP009273 982,570 IS5 (–) +4 bp 52.0% intergenic (‑132/+468) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 683307 =NA (NA)26 (0.450) 24/276 NT 51.3% noncoding (1195/1195 nt) IS5 repeat region
?CP009273 = 982573 24 (0.430)intergenic (‑135/+468) ompF/asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
* ? CP009273 = 684501NA (NA)26 (0.450) 25/276 NT 51.3% noncoding (1/1195 nt) IS5 repeat region
?CP009273 982570 = 24 (0.430)intergenic (‑132/+471) ompF/asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase

TCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTAC  >  CP009273/684374‑684650
                                                                                                                               |                                                                                                                                                      
tCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGc                                                                                                                                  >  2:96044/1‑150 (MQ=255)
      cTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGTTGACTCATGATGAACCCTGTTCTATGGCCCCCGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATg                                                                                                                            >  2:900215/1‑149 (MQ=255)
       tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATg                                                                                                                            >  2:343522/1‑149 (MQ=255)
           ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAg                                                                                                                       <  2:622907/150‑1 (MQ=255)
             cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGat                                                                                                                     <  1:934322/150‑1 (MQ=255)
             cgcTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGat                                                                                                                     <  2:157646/150‑1 (MQ=255)
               cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGatat                                                                                                                   <  1:893112/150‑1 (MQ=255)
                   ctgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTg                                                                                                              >  1:437584/1‑150 (MQ=255)
                    tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAATTGTAAAGATATTGCTg                                                                                                              >  1:360299/1‑150 (MQ=255)
                      ctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTg                                                                                                              <  2:972687/148‑1 (MQ=255)
                          aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTAt                                                                                                        <  1:290709/150‑1 (MQ=255)
                              aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTAttgttt                                                                                                   <  2:265062/150‑1 (MQ=255)
                                 gTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATtgtttgtt                                                                                                >  1:427306/1‑150 (MQ=255)
                                  tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATtgtttgtt                                                                                                <  2:383131/149‑1 (MQ=255)
                                     gCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGttttt                                                                                             >  2:528784/1‑150 (MQ=255)
                                       gAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGc                                                                                           >  2:661598/1‑150 (MQ=255)
                                          ggTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGc                                                                                           >  2:253019/1‑146 (MQ=255)
                                           gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGAt                                                                                      <  1:900215/150‑1 (MQ=255)
                                           gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGAt                                                                                      >  2:867927/1‑150 (MQ=255)
                                           gTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGAt                                                                                      <  1:571781/150‑1 (MQ=255)
                                                   atgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGcgccg                                                                                >  2:750905/1‑149 (MQ=255)
                                                    tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCa                                                                              <  1:688481/150‑1 (MQ=255)
                                                      aCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCAcc                                                                            <  2:539487/150‑1 (MQ=255)
                                                       cTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCa                                                                           <  2:819646/150‑1 (MQ=255)
                                                         cATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAtt                                                                         >  1:352854/1‑150 (MQ=255)
                                                         cATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAtt                                                                         >  1:555852/1‑150 (MQ=255)
                                                              tgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGaa                                                                    <  1:638756/150‑1 (MQ=255)
                                                              tgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAg                                                                   <  2:575813/150‑1 (MQ=255)
                                                                aaCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGc                                                                  <  1:96044/150‑1 (MQ=255)
                                                                 aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGc                                                                  >  1:624476/1‑149 (MQ=255)
                                                                  cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCaa                                                                >  2:168567/1‑149 (MQ=255)
                                                                     tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAAt                                                             >  1:257237/1‑150 (MQ=255)
                                                                            tGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCtt                                                         <  1:853839/147‑1 (MQ=255)
                                                                            tGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTaa                                                       >  2:18264/1‑149 (MQ=255)
                                                                                     aTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCtt                                            >  1:77470/1‑150 (MQ=255)
                                                                                       gACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCttt                                           >  2:405330/1‑150 (MQ=255)
                                                                                        aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCttt                                           >  2:794332/1‑149 (MQ=255)
                                                                                            aCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTg                                      <  1:522398/150‑1 (MQ=255)
                                                                                               tGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTa                                   <  2:555852/150‑1 (MQ=255)
                                                                                                  tctcATATCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCg                                >  2:964277/1‑150 (MQ=255)
                                                                                                        atCAGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGAt                          >  2:473523/1‑150 (MQ=255)
                                                                                                           aGGGACTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTa                        <  2:917328/149‑1 (MQ=255)
                                                                                                               aCTTGTTCGCACCTTCCTTAAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACtgtg                   <  2:302097/150‑1 (MQ=255)
                                                                                                                      cGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAAc            >  1:225556/1‑149 (MQ=255)
                                                                                                                              cctt‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTG‑TTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTAc  >  2:821556/1‑150 (MQ=255)
                                                                                                                               |                                                                                                                                                      
TCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT‑AGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAACTAGGTTGTAC  >  CP009273/684374‑684650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.