Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	CP009273	3,553,266	Δ8 bp	12.4%	coding (693‑700/759 nt)	glpR ←	GlpR DNA‑binding transcriptional repressor

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 3553265	38 (0.660)	5 (0.090)	5/268	NT	12.4%	coding (701/759 nt)	glpR	GlpR DNA‑binding transcriptional repressor
	?	CP009273	3553274 =	34 (0.610)					coding (692/759 nt)	glpR	GlpR DNA‑binding transcriptional repressor

TTTCGCGCCAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAA  >  CP009273/3553116‑3553411                                                                                                                                                      |                                                                                                                                                   tttCGCGCCAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCG                                                                                                                                                    <  1:809995‑M1/150‑1 (MQ=255)      cgcCAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCggg                                                                                                                                               >  2:610386/1‑150 (MQ=255)         cAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCg                                                                                                                                            >  1:141485/1‑150 (MQ=255)           gggCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTc                                                                                                                                          >  2:640973/1‑150 (MQ=255)           gggCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTc                                                                                                                                          <  1:298364/150‑1 (MQ=255)             gCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCgg                                                                                                                                        <  2:319300/150‑1 (MQ=255)               aaaGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGgtg                                                                                                                                      <  1:35491/150‑1 (MQ=255)                aaGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGtcggtgtagacggca                                                                                                                                     <  1:435782‑M1/150‑16 (MQ=255)                aaGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGgtg                                                                                                                                      >  1:851232/1‑149 (MQ=255)                   ccAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGtcggtgtagacggcatct                                                                                                                                  <  2:212315‑M1/150‑19 (MQ=255)                    cAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGAc                                                                                                                                 >  1:945485/1‑150 (MQ=255)                     aGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACg                                                                                                                                >  1:854138/1‑150 (MQ=255)                        gTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCCCAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCa                                                                                                                             >  1:521411/1‑150 (MQ=255)                           aaTGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCt                                                                                                                          <  2:431687/150‑1 (MQ=255)                               aCGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCa                                                                                                                      >  1:251074/1‑150 (MQ=255)                                cgcgGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCat                                                                                                                     <  2:934039/150‑1 (MQ=255)                                    gATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCatgctg                                                                                                                 <  1:506727/150‑1 (MQ=255)                                      tCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCatgctgat                                                                                                               <  1:918868/150‑1 (MQ=255)                                          cTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCatgctgatgctg                                                                                                           >  1:878494/1‑150 (MQ=255)                                            aCGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGcc                                                                                                         >  1:318044/1‑150 (MQ=255)                                                aaaTACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCata                                                                                                     >  1:931715/1‑150 (MQ=255)                                                 aaTACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCatat                                                                                                    <  2:521411/150‑1 (MQ=255)                                                    aCCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGa                                                                                                 >  2:977912/1‑150 (MQ=255)                                                      cTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGAcc                                                                                               <  2:945485/150‑1 (MQ=255)                                                         gcgcGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCAt                                                                                             <  2:878494/149‑1 (MQ=255)                                                             gTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGt                                                                                        <  1:640973/150‑1 (MQ=255)                                                                 tGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTAc                                                                                     <  2:56094/149‑1 (MQ=255)                                                                     cTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGtcggtgtagacggcatctaccatgctgatgctgcccatattgaccatcgcgttacggccaaatttc                                                                                  >  1:626142‑M1/1‑82 (MQ=255)                                                                        aCGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCa                                                                                <  2:865576/147‑1 (MQ=255)                                                                          gTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGcggcgg                                                                                                                                                >  2:363480/1‑81 (MQ=255)                                                                           tGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCggg                                                                                                                                               <  1:363480/81‑1 (MQ=255)                                                                                   cGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATc                                                                  <  1:259475/150‑1 (MQ=255)                                                                                   cGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATc                                                                  <  2:626575/150‑1 (MQ=255)                                                                                         ggATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGtcggtgtagacggcatctaccatgctgatgctgcccatattgaccatcgcgttacggccaaatttcgagtgatcgacaaccagcataa                                                            <  1:243414‑M1/150‑89 (MQ=255)                                                                                          gATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAAcc                                                           <  2:809499/150‑1 (MQ=255)                                                                                          gATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAAc                                                            >  2:296058/1‑149 (MQ=255)                                                                                              aGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCa                                                       >  2:645846/1‑150 (MQ=255)                                                                                                 acaGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATaa                                                    <  2:851232/150‑1 (MQ=255)                                                                                                        cAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGgc                                              <  1:554201/149‑1 (MQ=255)                                                                                                              aaTATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGtt                                       >  1:955766/1‑150 (MQ=255)                                                                                                              aaTATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGt                                        <  2:251074/149‑1 (MQ=255)                                                                                                                             cAGCACCTGCATCACGCTTACTGGCGccggtgtagacggcatctaccatgctgatgctgcccatattgaccatcgcgttacggccaaatttcgagtgatcgacaaccagcataacgtggcgcgagttctcaataatggcgcgtttggtgc                        >  2:301346‑M1/1‑26 (MQ=255)                                                                                                                              aGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGt                       >  2:214618/1‑150 (MQ=255)                                                                                                                                       aTCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCg               >  1:114045/1‑149 (MQ=255)                                                                                                                                        tCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACCTGGCGCGAGCTCTCAATAATGGCGCGGTTGGTGCGaa             >  2:239610/1‑150 (MQ=255)                                                                                                                                          aCGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAAc            <  2:854138/149‑1 (MQ=255)                                                                                                                                                 cTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGt    <  2:931715/150‑1 (MQ=255)                                                                                                                                                     cGtcggtgtagacggcatctaccatgctgatgctgcccatattgaccatcgcgttacggccaaatttcgagtgatcgacaaccagcataacgtggcgcgagttctcaataatggcgcgtttggtgcgaacttcgtggtaatcgaactc  >  2:671431‑M1/1‑2 (MQ=255)                                                                                                                                                     cGtcggtgtagacggcatctaccatgctgatgctgcccatattgaccatcgcgttacggccaaatttcgagtgatcgacaaccagcataacgtggcgcgagttctcaataatggcgcgtttggtgcgaacttcgtggtaatcgaactc  >  1:911209‑M1/1‑2 (MQ=255)                                                                                                                                                      |                                                                                                                                                   TTTCGCGCCAGGGCAAAGCCAGCGTCAATGACGCGGATCGGCTACGCAAATACCTGGCGCGTTTTGGTCTGACGTGGGAAGCCGTGCAGGATCAGCACAGCTCCAGTTGAATATGGTGGTCCGTCAGCACCTGCATCACGCTTACTGGCGGCGGGGCGTCGGTGTAGACGGCATCTACCATGCTGATGCTGCCCATATTGACCATCGCGTTACGGCCAAATTTCGAGTGATCGACAACCAGCATAACGTGGCGCGAGTTCTCAATAATGGCGCGTTTGGTGCGAACTTCGTGGTAA  >  CP009273/3553116‑3553411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.