Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,888 T→C 75.6% G217G (GGT→GGC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,8880TC75.6% 479.0 / 173.7 246G217G (GGT→GGC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (31/29);  new base C (90/96);  total (121/125)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 247 total aligned reads displayed.

GCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCG  >  CP009273/8739‑9036
                                                                                                                                                     |                                                                                                                                                    
    aaGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                                   >  2:963500/1‑149 (MQ=255)
    aaGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTAt                                                                                                                                                   >  2:655134/1‑149 (MQ=255)
     aGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGa                                                                                                                                                 <  1:390061/150‑1 (MQ=255)
     aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTGCAAGCGGCACGGCTATGa                                                                                                                                                 <  2:789607/150‑1 (MQ=255)
      gCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGaa                                                                                                                                                <  1:608718/150‑1 (MQ=255)
        gggCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAAc                                                                                                                                              <  2:924555/150‑1 (MQ=255)
        gggCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAAc                                                                                                                                              >  2:692373/1‑150 (MQ=255)
         ggCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAAcc                                                                                                                                             >  2:85872/1‑150 (MQ=255)
            gtgtTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTg                                                                                                                                          >  2:191127/1‑150 (MQ=255)
              cttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACcgtcgt                                                                                                                                        >  1:509737/2‑150 (MQ=255)
                 ccTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGAt                                                                                                                                     >  2:392603/1‑150 (MQ=255)
                  cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGAt                                                                                                                                     <  2:49345/149‑1 (MQ=255)
                   tGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATgg                                                                                                                                   >  1:350855/1‑150 (MQ=255)
                     atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATgg                                                                                                                                   <  1:300685/145‑1 (MQ=255)
                          gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAg                                                                                                                            <  2:184446/150‑1 (MQ=255)
                          gCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAg                                                                                                                            >  1:259481/1‑150 (MQ=255)
                            cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                                                                                                          >  1:174900/1‑150 (MQ=255)
                            cGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCt                                                                                                                          <  1:774251/150‑1 (MQ=255)
                                tgttgGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCg                                                                                                                      >  2:300686/1‑150 (MQ=255)
                                cgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTcc                                                                                                                       <  1:661291/148‑1 (MQ=255)
                                cgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                                                                                                      >  2:456057/2‑150 (MQ=255)
                                 gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                                                                                                      <  2:121822/149‑1 (MQ=255)
                                  ttgGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGt                                                                                                                     >  2:553252/1‑149 (MQ=255)
                                   tgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTaa                                                                                                                   >  1:271366/1‑150 (MQ=255)
                                    ggCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTaa                                                                                                                   <  1:454908/149‑1 (MQ=255)
                                       cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                                                                                                               >  1:472889/1‑147 (MQ=255)
                                       cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                                                                                                               >  1:125289/1‑147 (MQ=255)
                                       cGTATCCTCCACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgtt                                                                                                               >  1:441995/1‑147 (MQ=255)
                                        gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                                                                                                              >  1:194926/1‑146 (MQ=255)
                                        gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                                                                                                              <  2:218176/150‑5 (MQ=255)
                                         tATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttgg                                                                                                             >  2:681282/1‑145 (MQ=255)
                                          atcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGc                                                                                                            >  2:82483/7‑150 (MQ=255)
                                           tcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGc                                                                                                            <  2:15351/144‑1 (MQ=255)
                                           tcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGc                                                                                                            >  1:182441/6‑149 (MQ=255)
                                           tcctcgACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGc                                                                                                            <  2:516090/144‑1 (MQ=255)
                                               tGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAAt                                                                                                       >  2:291949/1‑150 (MQ=255)
                                                 aCTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTc                                                                                                     <  1:70965/150‑1 (MQ=255)
                                                   tGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTcc                                                                                                                       >  2:38115/1‑130 (MQ=255)
                                                   tGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTg                                                                                                   >  1:92060/1‑150 (MQ=255)
                                                    ggTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                                                                                                      <  1:38115/130‑1 (MQ=255)
                                                    ggTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTg                                                                                                   >  2:108953/1‑149 (MQ=255)
                                                        cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc                                                                                               <  2:441995/149‑1 (MQ=255)
                                                        cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt                                                                                              <  2:669406/150‑1 (MQ=255)
                                                            gccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGct                                                                                          <  1:415111/144‑2 (MQ=255)
                                                             ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                                                                                         >  2:718061/6‑148 (MQ=255)
                                                             ccaacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGctg                                                                                         <  1:324635/145‑3 (MQ=255)
                                                               aacaCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACtggctggc                                                                                       >  2:496307/4‑150 (MQ=255)
                                                                aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCt                                                                                      >  2:128207/1‑150 (MQ=255)
                                                                 caCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctg                                                                                     >  2:702661/2‑150 (MQ=255)
                                                                  aCCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGgctggctgt                                                                                    >  1:910595/1‑149 (MQ=255)
                                                                    cGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCAGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGa                                                                                  <  2:412994/150‑1 (MQ=255)
                                                                    cGACAAGAGAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGa                                                                                  >  1:627719/1‑150 (MQ=255)
                                                                     gacaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAc                                                                                 <  2:573989/147‑1 (MQ=255)
                                                                      acaagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGAcc                                                                                <  1:856969/148‑1 (MQ=255)
                                                                       caagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCg                                                                               <  1:812848/149‑1 (MQ=255)
                                                                       caagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCg                                                                               <  1:655134/149‑1 (MQ=255)
                                                                        aagaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgc                                                                              >  1:296841/1‑149 (MQ=255)
                                                                         agaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgcc                                                                             >  1:698538/1‑148 (MQ=255)
                                                                         agaaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCgcc                                                                             <  1:88324/150‑3 (MQ=255)
                                                                          gaaagaGTGCGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCt                                                                            <  1:677034/150‑1 (MQ=255)
                                                                           aaagaGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTg                                                                           <  2:401619/150‑1 (MQ=255)
                                                                           aaagaGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCt                                                                            >  2:644495/1‑149 (MQ=255)
                                                                               agTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCa                                                                       <  1:477083/150‑1 (MQ=255)
                                                                                gTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCAt                                                                      <  1:737096/150‑1 (MQ=255)
                                                                                 tACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATc                                                                     <  1:544988/150‑1 (MQ=255)
                                                                                 tACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATc                                                                     <  2:608991/150‑1 (MQ=255)
                                                                                   cGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGc                                                                   >  1:19191/1‑150 (MQ=255)
                                                                                       ccGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCg                                                               >  1:5661/1‑150 (MQ=255)
                                                                                         ggCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGc                                                             >  1:876761/1‑150 (MQ=255)
                                                                                          gCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcg                                                            >  2:262893/1‑149 (MQ=255)
                                                                                            agaagaTCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGct                                                          >  2:347502/1‑150 (MQ=255)
                                                                                              aagaTCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGctgc                                                        >  2:813679/1‑150 (MQ=255)
                                                                                               agaTCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCgctgct                                                       >  2:220804/1‑150 (MQ=255)
                                                                                                gaTCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCActgct                                                       >  1:367063/1‑149 (MQ=255)
                                                                                                      ggCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAagag                                                <  2:897411/150‑1 (MQ=255)
                                                                                                        cGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCt                                              <  1:964113/150‑1 (MQ=255)
                                                                                                        cGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCt                                              <  2:768397/150‑1 (MQ=255)
                                                                                                         gTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTg                                             >  2:883610/1‑150 (MQ=255)
                                                                                                         gTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTg                                             <  1:87378/150‑1 (MQ=255)
                                                                                                           ggTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGc                                           >  1:361706/1‑150 (MQ=255)
                                                                                                            gTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCg                                          >  1:837206/1‑150 (MQ=255)
                                                                                                                   tATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGc                                    <  2:367063/149‑1 (MQ=255)
                                                                                                                    cagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGaaa                                                   >  1:726115/5‑133 (MQ=255)
                                                                                                                     agcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAag                                                  <  2:726115/130‑1 (MQ=255)
                                                                                                                      gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                                >  1:507377/3‑150 (MQ=255)
                                                                                                                      gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                                >  2:352457/3‑150 (MQ=255)
                                                                                                                       cgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAgg                               <  1:637722/149‑1 (MQ=255)
                                                                                                                        gAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAggc                              <  2:259481/150‑2 (MQ=255)
                                                                                                                         aaaTCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAgg                               <  2:688492/148‑1 (MQ=255)
                                                                                                                          aaTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgc                            <  1:333245/150‑1 (MQ=255)
                                                                                                                             cTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgccgt                         >  2:170565/1‑147 (MQ=255)
                                                                                                                             cTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTAt                         >  2:748701/1‑150 (MQ=255)
                                                                                                                                ccAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTa                          <  1:574502/146‑1 (MQ=255)
                                                                                                                                     actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                  >  1:433970/1‑149 (MQ=255)
                                                                                                                                     actacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGt                  >  1:732856/1‑149 (MQ=255)
                                                                                                                                         caagcagCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTaagc              <  1:108953/144‑3 (MQ=255)
                                                                                                                                           agcagCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTgg           >  2:533616/4‑149 (MQ=255)
                                                                                                                                           aaGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTaa                >  2:62399/1‑145 (MQ=255)
                                                                                                                                                cACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTggtatat      <  1:98028/150‑7 (MQ=255)
                                                                                                                                                cACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTggtatat      <  1:106771/150‑7 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCG  >  CP009273/8739‑9036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑