| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,147 | G→C | 23.9% | intergenic (‑81/+514) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,147 | 0 | G | C | 23.9% | 204.4 / 18.9 | 84 | intergenic (‑81/+514) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (33/30); new base C (17/4); total (50/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATT > CP009273/360002‑360293 | tCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGcc < 2:126983/150‑1 (MQ=255)gatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGcc > 1:168224‑M1/103‑150 (MQ=255) atttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCg > 1:864272‑M1/102‑150 (MQ=255) tttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGt > 2:111959‑M1/101‑150 (MQ=255) ggCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAGTTCCTGGCAGTTTATGGCGGG‑CATCCTGCCCGCCACCCTCCGGGCCGTTGCt < 2:863516/150‑1 (MQ=255) aggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACg > 1:169555‑M1/89‑150 (MQ=255) aCCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGCCCTGCCCCCCCCCCCCCCCGCCGCTGCTTCG‑Caaaa > 2:720895/1‑148 (MQ=255) ccGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGt < 2:290759/150‑1 (MQ=255) ccGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGGCCTGCCCGCCCCCCCCCCGGCCCTTTCTTCG‑CAAcct > 2:426135/1‑148 (MQ=38) gagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCa > 1:689177‑M1/85‑150 (MQ=255) tcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑GGTCCCGCCCCCCCCCCCCCCGGCCGGTGCTTCT‑CAAAGTTCAAAtccc < 2:187899‑M1/72‑2 (MQ=255) aGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCg < 1:656195/150‑1 (MQ=255) aGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCCCCCCCCCCCGGGCCGTTGCTTCG‑CCACGTTCAAATca > 1:826266/1‑148 (MQ=255) aGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGC‑GGGCCCGCCCGCCCCCCCCCCCGGCGCTTCTTCG‑CAACGCTCAAAACCg > 2:670652/1‑150 (MQ=255) caccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCg > 2:512486‑M1/78‑149 (MQ=255) cGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCt < 2:441631/150‑1 (MQ=255) ggCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTc < 1:602273/150‑1 (MQ=255) caaacatcagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCcgg > 2:687465‑M1/72‑150 (MQ=255) aaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCcggc > 2:87613‑M1/71‑150 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCcggc < 2:141533/149‑1 (MQ=255) aacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCcggc‑g > 2:650936‑M1/70‑150 (MQ=255) cATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCcggc‑gg < 2:859350/150‑1 (MQ=255) aTCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGa < 1:802050/150‑1 (MQ=255) aacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGAt > 1:324786‑M1/67‑150 (MQ=255) acagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGAtt > 2:870323‑M1/66‑150 (MQ=255) cagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGAttt > 2:765315‑M1/65‑150 (MQ=255) aGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTg < 1:400947/150‑1 (MQ=255) caccaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTa < 1:428166/150‑1 (MQ=255) caccaTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTCCCAGCCACCCCCCCGGGCGGTTCTTCG‑CAAAGTTCAAAACCGCTCCCGGC‑GGGGTTGTTCtt > 1:670058/1‑149 (MQ=25) ccaTCCAGTGCGGGACCTCCGATGATACGCTGTCAGACATGAGAATTCCAATCCCCAATTCCTGGCGGTTTATGACGGG‑CGGCCTGCCCGCCACCCTCCGGACCGTTGCTTCG‑CAACGTTCAGATCCGCTCCCAGC‑GGATTTGTCCTACt < 2:809301/150‑1 (MQ=18) tCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAg < 1:981376/150‑1 (MQ=255) ccAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCCGCCCCCCCCCCCCCCGGCCCGTGCCTTG‑CAAAGTTCAAATCCGCTCCCGGC‑GGGTGTGTCCTACTctg > 2:864272/1‑147 (MQ=21) aggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGga > 1:588050‑M1/52‑150 (MQ=255) gTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGGCCTGCCCCCCACCCCCCCCGCCGTTGCTTTGCCAACTTTCAAA‑CCCCCCCCCGC‑GGGTTTGTCCTCCTCacgg > 2:177472/1‑145 (MQ=255) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGGCCTGCCCGCCCCCCCCCCGGGCGGTGCTTCG‑CAAAGTTGAAAATCGCTCCCCGC‑GGATTTGTTCTAATCAGGggag < 2:956617‑M1/102‑1 (MQ=255) cAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGc < 1:909252/149‑1 (MQ=255) cAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCg < 2:7081/150‑1 (MQ=255) ggAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCCCCCCCCCGGCCGGTGCTTCG‑CAACCTTCAAAACCGCCCCCCGC‑GGGTTTTTCCTTCTCCGGAGAGCggt > 1:292077/1‑148 (MQ=21) tctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTc > 2:556551‑M1/44‑150 (MQ=255) gCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCCGCCCGCCACCCCCCCGGCCGCCGCTTCG‑CAAAGTTCAAATCCCCCCCCCGCGGGGTTTTTCCT‑CTCCGGAGAGCGtttcc > 1:870323/1‑147 (MQ=18) cTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCAcc < 1:426135/150‑1 (MQ=255) gactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGGCCTGCCCCCCCCCCTCCCGGCCGTTGCTTCG‑CAACATTCAAATCCCCTCCCCGC‑GGGTTTTTCCTACTCAGGAGAgagttccccca < 1:925558‑M1/112‑6 (MQ=255) cGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCCGCCCCCCACCCCCCGGGCCGGTGCTTCG‑CAACGTTTCAATCCCCCCCCCGC‑GGGTTTTTTCTACTCAGGAGAGCGTTTAccca > 1:628686/1‑147 (MQ=18) gatgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCCGCCCGGCCCCCCCCCCCCCGCG‑CGTTTCTTCT‑CCACCTTTACATCCCCCCCCCGC‑CGGCTTGTTCTTCTCCGGGGAGGGGTCaaccgc > 2:324786/1‑144 (MQ=255) actgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGaca > 2:934028‑M1/37‑150 (MQ=255) tgatAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCCGCCCCCCACCCCCCCGGCCGGTGCTTTG‑CCAAGTTCAAAAACGCCCCCGGC‑GGGGTTTTCCTACTCCGGAGAGAGGTCACCCacaa > 2:471044/1‑149 (MQ=18) aGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAaacaac < 2:785151/149‑1 (MQ=255) ttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaa > 1:471044‑M1/24‑150 (MQ=255) tttatttgatgcctggggaattAATTCCTGGCAGTTTATGGTGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa < 2:405342‑M1/128‑1 (MQ=255) ttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa > 1:67004‑M1/22‑149 (MQ=255) ttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaaaa > 1:689656‑M1/22‑149 (MQ=255) aCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 2:187015/150‑1 (MQ=255) atttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg > 1:187899‑M1/20‑149 (MQ=255) aTGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGaa < 1:177472/150‑1 (MQ=255) tGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGaaa < 1:835220/150‑1 (MQ=255) tgatgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑GGGCCTTCCCCCCACCCCCCCGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGGTTTGTCCTACTCAGGAGAGCGTTCACCGACAAAAAACAAATAAAACGaa < 1:778234‑M1/132‑1 (MQ=255) gatgcctggggaattAATTCTTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg > 2:452127‑M1/16‑149 (MQ=255) atgcctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAgg > 1:938119‑M1/15‑149 (MQ=255) aaTTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCCCCCCCCCCCCGGCCCCTGCTTCT‑CCACCCTCCAATCCCCCCCCCGC‑GGGTTTTTCCCTCTCACGAGAGCGTTCACCCCCCAACaaaagaaaaaaagaaaaacc > 2:442867/1‑146 (MQ=9) ctggggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg > 2:319768‑M1/11‑150 (MQ=255) ccGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAg < 1:735813/149‑1 (MQ=40) gggaattAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt > 1:136869‑M1/8‑150 (MQ=255) gATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt < 1:877977/150‑1 (MQ=38) ggaattaaTTCCTGGCAGTTTATGGCGGG‑CGTCCTTCCCGCCCCCCCCCCTGCCCGTGTTTTT‑CAACCATTAAATCCGCCCCCCGC‑GGGGTTGTTCTCCTTATGAGAGCGTTTCCCCCCAAACAACAGAAAAAAAAAAAAGACCAATCtt > 2:593756/7‑150 (MQ=2) tCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCttt < 1:459376/150‑1 (MQ=37) ccccAATTCCTGGCAGTTTATGGCGGG‑CGTCCCGCCCGCCCCCCCCCGGGCCGGTGCTTCG‑CAAAGTTCAAATCCGCTCCCCGC‑GGGTTTGTTCTACTCAGGAGAGGGGTTACCCACAAAAAAAAAAAAAAAAGAAAAGGCCAGTCCTTc > 1:138599/1‑150 (MQ=2) aattaaTTCCTGGCAGTTTATGGCGGG‑CGCCCTTCCCCCCACCCTCCCGGGCCTTTCCTCG‑CAACGCTCCAATCCCCTCCCCGC‑GCATTTTTCCTCCTCCCGAGAGCGTTCCCCCACAAACCACTGTTTAAACGAAAAGCCCAGCCTTTc > 1:565461/5‑150 (MQ=2) cccAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc < 2:428065/149‑1 (MQ=35) attAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCg > 1:931196‑M1/4‑150 (MQ=255) aaTTCCTGGCAGTTTATGGCGGG‑TGCCCCGCCCCCCACCCCCCCGGGCGGTGTTTCT‑CAAAGTTTAAAACCGCTCCCCGC‑GGATTTATCCTTCTCTCGAGGGAGTGGACAGACAAAAAACAGATAAAAAGATAGGCTCAGTCatttgcgt > 2:69124/1‑145 (MQ=2) aaTTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACt < 2:292576/150‑1 (MQ=32) aTTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCCCCCCCCCCCCGGCCGGTGCTTCG‑CAAAATTTAAAATCCCTCCCCGC‑GGGGTTTTTCTTCTCAAGGGGGGGTTCTCCCCCAAACAAAAAATAAAAAAAAAAGGCCACTCtttttact > 2:605836/1‑144 (MQ=2) ttCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCCCCACCCCCCCGGCCCTTGCTTCG‑CAACAATTAAATCCCCTCCCCGC‑GGGGTTTTTCTACTCCAGGGAGGGGTTCCCCACAAAAAAAAAAAAAAAAAAAAAGGCCCGTCTTTCGACTgc > 2:665759/1‑149 (MQ=2) ttCCTGGCAGTTTATGGCGGG‑CGGCCTTCCCCCCCCCCTCCCGGGCGTTGCTTCG‑CAACGTTCAAATCCCCTCCCCGC‑GGGGTTTTCCTTCTCAGGAGAGCGGTTACCCACAAACAAAAGATAAAAAGAAAAGCCCAGTCtttttact > 1:650936/1‑143 (MQ=2) tCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCCCCCCCCGGGCCGGTGCTTCG‑CAACGTTCAAATCCCCTCCCCGC‑GGGTTTTTCCTTCTCAGGAGAGCGTTCACCCACAAACAACAGATAAAACGAAAAGCCCAGGCTTTCGACTGAg > 1:41480/1‑150 (MQ=11) tCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCCCCACCCCCCCGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCCGC‑GGGTTTTTCCTACTCAGGAGAGCGGTCACCCACAAAAAACAGATAAAAAAAAAAGCCCCGTCTTTCTACTGAg > 1:448743/1‑150 (MQ=11) gCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTggg > 2:479684/1‑149 (MQ=11) cccGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTgggg > 2:28080/1‑149 (MQ=31) cgacACCCTGCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAGCGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat < 2:223351/147‑1 (MQ=16) cGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaat > 2:251238/1‑150 (MQ=33) gCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaatt > 1:357461/1‑150 (MQ=34) aCCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattggg < 2:972896/150‑1 (MQ=37) ccTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattgggaa < 1:238075/150‑1 (MQ=38) cGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGgggaattgggaatt < 1:498112/148‑1 (MQ=40) | TCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGG‑CGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCG‑CAACGTTCAAATCCGCTCCCGGC‑GGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATT > CP009273/360002‑360293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |