| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,909,758 | C→A | 20.7% | E12* (GAA→TAA) | proQ ← | RNA chaperone, putative ProP translation regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,909,758 | 0 | C | A | 20.7% | 147.3 / 28.1 | 53 | E12* (GAA→TAA) | proQ | RNA chaperone, putative ProP translation regulator |
| Reads supporting (aligned to +/- strand): ref base C (24/18); new base A (5/6); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
AAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATCCTGCGACGCTCG > CP009273/1909610‑1909904 | aaaGCGGATCGCAATTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtct > 2:888759/1‑150 (MQ=255) aaGCGGATCGCAATTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTActtct < 1:242036/149‑1 (MQ=255) aGCGGATCGCAATTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCttt < 1:960758/150‑1 (MQ=255) tCGCAATTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCt < 1:768391/150‑1 (MQ=255) aTTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTc > 2:267072/1‑149 (MQ=255) aTTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCa < 2:699555/150‑1 (MQ=255) tGCGTTTTGCTCAGGTTCATTTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCaa > 1:630146/1‑150 (MQ=255) gCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACt > 1:913654/1‑150 (MQ=255) cGTTTTGCTCAGGTTCATTTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACt > 1:768018/1‑150 (MQ=255) gCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGtt < 1:108140/149‑1 (MQ=255) tCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAt > 2:430776/1‑150 (MQ=255) ggTTCATTTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGAttt > 1:444060/1‑150 (MQ=255) ttCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTa > 1:330241/1‑129 (MQ=255) tcattttCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTc > 2:253655/5‑149 (MQ=255) tCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTAt < 2:330241/129‑1 (MQ=255) a‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATg < 2:763680/150‑1 (MQ=255) ttCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGa > 1:353967/1‑149 (MQ=255) tCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGaa < 1:65538/149‑1 (MQ=255) ccccAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAAt > 1:243912/1‑150 (MQ=255) aGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTcc > 2:554769/1‑150 (MQ=255) aaCACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGa > 2:348643/1‑150 (MQ=255) aaCACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGa > 2:784339/1‑150 (MQ=255) aTCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACaa < 1:784339/150‑1 (MQ=255) aCCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGa > 1:109897/1‑150 (MQ=255) cTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGcc > 2:521140/1‑149 (MQ=255) aaaaaTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCcaac < 1:253655/149‑1 (MQ=255) aaaTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAg < 1:62268/150‑1 (MQ=255) aTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAgcg < 1:711581/150‑1 (MQ=255) aTACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAgcg > 1:393030/1‑150 (MQ=255) ccGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCa < 2:878837/149‑1 (MQ=255) ccGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAg < 1:109504/150‑1 (MQ=255) gATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAgg > 2:10279/1‑149 (MQ=255) ttttCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCAt > 2:881037/1‑150 (MQ=255) aGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACa < 2:884652/150‑1 (MQ=255) cgcgCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACACGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTc < 1:214252/150‑1 (MQ=255) gcgcTTTACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCa > 1:319364/1‑150 (MQ=255) gcTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCAtt < 1:430776/150‑1 (MQ=255) gcTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCAt > 2:309277/1‑149 (MQ=255) aCCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAAt > 1:893199/1‑150 (MQ=255) ccTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAAt > 1:75809/1‑149 (MQ=255) ccTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAAt > 1:642124/1‑149 (MQ=255) gCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTa > 2:209980/1‑150 (MQ=255) aCTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATc < 1:348643/150‑1 (MQ=255) aaCAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTa > 2:43218/1‑149 (MQ=255) cAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAAt < 1:888759/150‑1 (MQ=255) cAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAAt < 2:153538/150‑1 (MQ=255) aaCGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTAt < 1:810541/150‑1 (MQ=255) cGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGtt < 2:913654/149‑1 (MQ=255) ccAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGAt > 2:244641/1‑149 (MQ=255) cAGAAACGCGATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGCAAATGCTACGTTATCCGTTGATTa > 2:328748/1‑150 (MQ=255) aaaCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATcc > 2:706003/1‑150 (MQ=255) aaaCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATcc > 1:775891/1‑150 (MQ=255) aCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATCCTg < 1:309277/150‑1 (MQ=255) gcgATTACTTATTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATCCTGCg < 1:70757/150‑1 (MQ=255) aTTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATCCTGCGACg < 2:881538/150‑1 (MQ=255) cttcttTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATCCTGCGACGCTCg > 2:248165/1‑150 (MQ=255) | AAAGCGGATCGCAATTGCGTTTTGCTCAGGTTCA‑TTTCCCCAGCAACACGATCGACCAAATCCTGAAAAATACCGATTTTCAGCGGACGCGCTTCACCTTCCGCACTGAAACAGTGGGGAAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTATCAGTAAATGCTACGTTATCCGTTGATTATCCTGCGACGCTCG > CP009273/1909610‑1909904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |