Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,074,455 T→G 11.4% A180A (GCA→GCC)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,074,4550TG11.4% 677.5 / 73.5 220A180A (GCA→GCC) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (90/105);  new base G (9/16);  total (99/121)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01

Only 100 of 224 total aligned reads displayed.

TCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTT  >  CP009273/3074306‑3074603
                                                                                                                                                     |                                                                                                                                                    
tcttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAAt                                                                                                                                                      >  2:793890/1‑150 (MQ=255)
 cttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAAt                                                                                                                                                      <  1:32056/149‑1 (MQ=255)
 cttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATg                                                                                                                                                     >  2:858223/1‑150 (MQ=255)
    ctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATg                                                                                                                                                     <  2:654668/147‑1 (MQ=255)
     tACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt                                                                                                                                                 >  1:284376/1‑150 (MQ=255)
       cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt                                                                                                                                                 >  2:826694/1‑148 (MQ=255)
       cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATc                                                                                                                                                <  1:12782/149‑1 (MQ=255)
          cgcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAg                                                                                                                                              <  1:110028/148‑1 (MQ=255)
          cgcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAg                                                                                                                                              <  2:418926/148‑1 (MQ=255)
          cgcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGtt                                                                                                                                            <  1:346824/150‑1 (MQ=255)
          cgcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGt                                                                                                                                             <  2:355470/149‑1 (MQ=255)
           gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGttt                                                                                                                                           <  1:42311/150‑1 (MQ=255)
           gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGtt                                                                                                                                            >  2:402112/1‑149 (MQ=255)
           gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGtt                                                                                                                                            >  1:560106/1‑149 (MQ=255)
             gTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTAc                                                                                                                                         <  2:255559/150‑1 (MQ=255)
             gTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTAc                                                                                                                                         <  1:692481/150‑1 (MQ=255)
              tttGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTAcc                                                                                                                                        <  1:277343/150‑1 (MQ=255)
               ttGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTAcc                                                                                                                                        >  2:888038/1‑149 (MQ=255)
                   tAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCt                                                                                                                                   <  1:447596/150‑1 (MQ=255)
                     gATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTc                                                                                                                                 <  2:942806/150‑1 (MQ=255)
                      aTGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCtt                                                                                                                                  >  1:857643/1‑148 (MQ=255)
                        gCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGc                                                                                                                              >  1:928766/1‑150 (MQ=255)
                         ccGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg                                                                                                                             <  2:457440/150‑1 (MQ=255)
                         ccGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAAACAGCCTGCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGc                                                                                                                              <  2:640673/149‑1 (MQ=255)
                            cgTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTAc                                                                                                                          >  2:204120/1‑150 (MQ=255)
                            cgTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTAc                                                                                                                          <  1:602726/150‑1 (MQ=255)
                             gTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTAcc                                                                                                                         <  2:368197/150‑1 (MQ=255)
                               cATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCg                                                                                                                       >  2:752597/1‑150 (MQ=255)
                                 tGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCCTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGc                                                                                                                      >  1:801200/1‑149 (MQ=255)
                                  gACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGAGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGc                                                                                                                      >  2:120595/1‑148 (MQ=255)
                                   aCCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGc                                                                                                                      >  1:405047/1‑147 (MQ=255)
                                    ccGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCa                                                                                                                    >  2:495578/1‑148 (MQ=255)
                                            gTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAAc                                                                                                          >  2:378909/1‑150 (MQ=255)
                                                cgAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCg                                                                                                      >  1:85863/1‑150 (MQ=255)
                                                  aaTAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTGCCCAGCTTCAGCGTACCCGCCAGGGAGCAAACTTCGTg                                                                                                    <  2:182447/150‑1 (MQ=255)
                                                   aTAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTgg                                                                                                   >  2:933769/1‑150 (MQ=255)
                                                       cGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTgg                                                                                                   >  1:367928/1‑146 (MQ=255)
                                                       cGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTgg                                                                                                   >  1:31584/1‑146 (MQ=255)
                                                        gTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTAAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGATTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGAt                                                                                              <  1:419537/150‑1 (MQ=255)
                                                         tGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATg                                                                                             <  1:634938/150‑1 (MQ=255)
                                                           ccAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGcc                                                                                           >  2:659254/1‑150 (MQ=255)
                                                             aGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTGCCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCtt                                                                                         <  1:121695/150‑1 (MQ=255)
                                                             aGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCtt                                                                                         <  1:143763/150‑1 (MQ=255)
                                                               ccGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc                                                                                       <  1:511662/150‑1 (MQ=255)
                                                                 gTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcat                                                                                     <  2:134425/150‑1 (MQ=255)
                                                                 gTAAGCTTCGAAACGCATTACGGTATCGTCGGTGAACCTGCCTTCAACGTTACCATCGATAGAAATACCATTGTCATCATAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcat                                                                                     <  2:635512/150‑1 (MQ=255)
                                                                    aGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcatcat                                                                                  <  2:284376/150‑1 (MQ=255)
                                                                    aGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcatcat                                                                                  <  1:914990/150‑1 (MQ=255)
                                                                     gCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATAAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATg                                                                                 <  2:583538/150‑1 (MQ=255)
                                                                          gAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGcc                                                                            <  2:870478/150‑1 (MQ=255)
                                                                           aaaCGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCg                                                                           <  2:146263/150‑1 (MQ=255)
                                                                             aCGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTc                                                                         <  2:940400/150‑1 (MQ=255)
                                                                              cGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCg                                                                        >  2:214915/1‑150 (MQ=255)
                                                                              cGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCg                                                                        >  1:75181/1‑150 (MQ=255)
                                                                               gCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGc                                                                       >  1:362303/1‑150 (MQ=255)
                                                                                 aTTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGcc                                                                      >  1:757837/1‑149 (MQ=255)
                                                                                   tGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCAt                                                                   >  2:433109/1‑150 (MQ=255)
                                                                                      ggTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg                                                                  >  1:891596/1‑148 (MQ=255)
                                                                                       gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAg                                                               >  1:306726/1‑150 (MQ=255)
                                                                                       gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAg                                                               <  1:724363/150‑1 (MQ=255)
                                                                                          tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc                                                             <  1:711125/149‑1 (MQ=255)
                                                                                            gtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGt                                                           <  2:614684/149‑1 (MQ=255)
                                                                                              cgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAgg                                                        >  2:723968/1‑150 (MQ=255)
                                                                                               ggTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgt                                                       <  1:987249/150‑1 (MQ=255)
                                                                                               ggTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgt                                                       >  1:231085/1‑150 (MQ=255)
                                                                                                 tGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtgt                                                     <  2:320645/150‑1 (MQ=255)
                                                                                                  gAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg                                                      >  1:478643/1‑148 (MQ=255)
                                                                                                       aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGt                                               >  1:163099/1‑150 (MQ=255)
                                                                                                       aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGt                                               >  2:318144/1‑150 (MQ=255)
                                                                                                             cAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGAc                                           <  1:248355/148‑1 (MQ=255)
                                                                                                             cAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACa                                          <  1:387811/149‑1 (MQ=255)
                                                                                                              aaCGTGCCCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt                                        <  1:100786/150‑1 (MQ=255)
                                                                                                              aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt                                        <  1:492790/150‑1 (MQ=255)
                                                                                                              aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt                                        >  2:501343/1‑150 (MQ=255)
                                                                                                               aCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATAAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATg                                       <  1:640021/150‑1 (MQ=255)
                                                                                                                cGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt                                      <  1:826694/150‑1 (MQ=255)
                                                                                                                 gTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGCACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTc                                     >  2:567910/1‑150 (MQ=255)
                                                                                                                      cATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGc                                >  1:186016/1‑150 (MQ=255)
                                                                                                                       aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGcc                               <  1:255112/150‑1 (MQ=255)
                                                                                                                          gATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCgg                            <  1:544227/150‑1 (MQ=255)
                                                                                                                           aTAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGa                           >  2:838718/1‑150 (MQ=255)
                                                                                                                                aaTACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGt                       >  2:830168/1‑149 (MQ=255)
                                                                                                                                  tgttgTTGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGttg                     >  1:555508/5‑148 (MQ=255)
                                                                                                                                    ccGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAAc                  <  1:933769/150‑1 (MQ=255)
                                                                                                                                      gTTGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTgaatt                 <  2:168267/149‑3 (MQ=255)
                                                                                                                                      gTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTg                <  2:757837/150‑1 (MQ=255)
                                                                                                                                       ttGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTg                <  2:465480/149‑1 (MQ=255)
                                                                                                                                         gtcgtcGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggcc             >  1:332955/1‑147 (MQ=255)
                                                                                                                                          tcgtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggccc            <  1:887582/147‑5 (MQ=255)
                                                                                                                                          tcgtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggcc             <  2:782388/146‑4 (MQ=255)
                                                                                                                                          tCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTgcgc             <  2:928962/149‑1 (MQ=255)
                                                                                                                                          tCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgcc            <  1:630848/150‑1 (MQ=255)
                                                                                                                                           cATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgccg           >  2:860046/1‑150 (MQ=255)
                                                                                                                                            aTCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgccgc          >  2:313193/1‑150 (MQ=255)
                                                                                                                                             tCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCgccgcc         <  1:793890/150‑1 (MQ=255)
                                                                                                                                               gTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAg       >  2:298891/1‑150 (MQ=255)
                                                                                                                                               gTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAg       >  2:338893/1‑150 (MQ=255)
                                                                                                                                                tAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGc      >  1:150919/1‑150 (MQ=255)
                                                                                                                                                   aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtt   >  2:188454/1‑150 (MQ=255)
                                                                                                                                                    aTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGt                                      >  2:269175/1‑114 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTT  >  CP009273/3074306‑3074603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑