Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,074,478 G→T 14.5% L173M (CTG→ATG)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,074,4780GT14.5% 740.6 / 99.0 240L173M (CTG→ATG) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (97/108);  new base T (14/21);  total (111/129)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-02

Only 100 of 241 total aligned reads displayed.

GCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTG  >  CP009273/3074330‑3074627
                                                                                                                                                    |                                                                                                                                                     
 ccGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGc                                                                                                                                                      <  2:793057/149‑1 (MQ=255)
 ccGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg                                                                                                                                                     <  2:457440/150‑1 (MQ=255)
    cgTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTAc                                                                                                                                                  <  1:602726/150‑1 (MQ=255)
     gTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTAcc                                                                                                                                                 <  2:368197/150‑1 (MQ=255)
      tCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTAccc                                                                                                                                                <  2:344295/150‑1 (MQ=255)
            ccGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCa                                                                                                                                            >  1:335881/1‑148 (MQ=255)
             cGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCagag                                                                                                                                         <  1:55886/150‑1 (MQ=255)
              gTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCaga                                                                                                                                          >  2:133137/1‑148 (MQ=255)
                  aTGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCa                                                                                                                                     >  1:134865/1‑149 (MQ=255)
                      cgcgAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACtt                                                                                                                                >  2:248355/1‑150 (MQ=255)
                       gcgAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTc                                                                                                                               <  2:621223/150‑1 (MQ=255)
                           aTAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTgg                                                                                                                           <  2:137039/150‑1 (MQ=255)
                              aCGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGga                                                                                                                         <  2:944194/149‑1 (MQ=255)
                               cGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTgg                                                                                                                           >  1:31584/1‑146 (MQ=255)
                               cGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTgg                                                                                                                           >  1:367928/1‑146 (MQ=255)
                                 tGCCAGCCGTAAGCTTCGAACCGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACGTCGTGGGAGATg                                                                                                                     <  1:95615/150‑1 (MQ=255)
                                   ccAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGcc                                                                                                                   >  2:659254/1‑150 (MQ=255)
                                     aGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTGCCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCtt                                                                                                                 <  1:121695/150‑1 (MQ=255)
                                       ccGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTcc                                                                                                               <  1:511662/150‑1 (MQ=255)
                                         gTAAGCTTCGAAACGCATTACGGTATCGTCGGTGAACCTGCCTTCAACGTTACCATCGATAGAAATACCATTGTCATCATAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcat                                                                                                             <  2:635512/150‑1 (MQ=255)
                                            aGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCcatcat                                                                                                          <  1:888038/150‑1 (MQ=255)
                                             gCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATAAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATg                                                                                                         <  2:583538/150‑1 (MQ=255)
                                              cTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATg                                                                                                         >  2:327689/1‑149 (MQ=255)
                                                  gAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGcc                                                                                                    >  1:320645/1‑150 (MQ=255)
                                                    aaCGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGt                                                                                                  >  1:639901/1‑150 (MQ=255)
                                                     aCGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTc                                                                                                 <  1:177173/150‑1 (MQ=255)
                                                      cGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCg                                                                                                >  1:75181/1‑150 (MQ=255)
                                                       gCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGc                                                                                               >  1:159722/1‑150 (MQ=255)
                                                             cGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg                                                                                          >  1:147498/1‑149 (MQ=255)
                                                              ggTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAGTCAGTTTGCCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGaa                                                                                        <  1:926144/150‑1 (MQ=255)
                                                              ggTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATg                                                                                          >  2:832655/1‑148 (MQ=255)
                                                               gtgtCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAg                                                                                       >  1:306726/1‑150 (MQ=255)
                                                                  tcgtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGc                                                                                     <  2:866236/149‑1 (MQ=255)
                                                                    gtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCg                                                                                    >  2:753509/1‑148 (MQ=255)
                                                                    gtcgGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGt                                                                                   <  2:85863/149‑1 (MQ=255)
                                                                       ggTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgt                                                                               >  1:231085/1‑150 (MQ=255)
                                                                       ggTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgt                                                                               >  1:756534/1‑150 (MQ=255)
                                                                          gAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGgtg                                                                              >  1:478643/1‑148 (MQ=255)
                                                                              cAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTgg                                                                        <  2:393652/150‑1 (MQ=255)
                                                                               aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGt                                                                       <  2:937102/150‑1 (MQ=255)
                                                                               aGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGt                                                                       >  2:318144/1‑150 (MQ=255)
                                                                                gCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTc                                                                      <  1:85235/150‑1 (MQ=255)
                                                                                      aaCGTGCCCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt                                                                <  1:100786/150‑1 (MQ=255)
                                                                                      aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt                                                                <  1:492790/150‑1 (MQ=255)
                                                                                      aaCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAAt                                                                >  2:501343/1‑150 (MQ=255)
                                                                                       aCGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATg                                                               >  1:988740/1‑150 (MQ=255)
                                                                                             ccATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTgg                                                         <  1:393888/150‑1 (MQ=255)
                                                                                               aTCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGcc                                                       <  1:255112/150‑1 (MQ=255)
                                                                                                tCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTCCCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGccc                                                      <  2:694989/150‑1 (MQ=255)
                                                                                                  gATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCgg                                                    <  1:544227/150‑1 (MQ=255)
                                                                                                   aTAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGa                                                   >  1:631151/1‑150 (MQ=255)
                                                                                                   aTAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGa                                                   >  2:838718/1‑150 (MQ=255)
                                                                                                      gAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACgg                                                <  1:29298/150‑1 (MQ=255)
                                                                                                         atgttgTTGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGttg                                             <  2:174010/145‑2 (MQ=255)
                                                                                                         atgttgTTGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGtt                                              >  1:734332/6‑149 (MQ=255)
                                                                                                          tACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTaa                                            <  2:283670/150‑1 (MQ=255)
                                                                                                           gttgTTGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTgaa                                           >  1:189881/4‑150 (MQ=255)
                                                                                                            ccGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAAc                                          <  1:933769/150‑1 (MQ=255)
                                                                                                             cGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACt                                         <  2:163099/150‑1 (MQ=255)
                                                                                                              gTTGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTgaatt                                         <  2:734332/149‑3 (MQ=255)
                                                                                                               ttGTCGTCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTg                                        <  2:84546/149‑1 (MQ=255)
                                                                                                                 gtcgtcGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggcc                                     >  1:908445/1‑147 (MQ=255)
                                                                                                                  tcgtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggccc                                    <  1:887582/147‑5 (MQ=255)
                                                                                                                  tcgtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggcc                                     <  2:916540/146‑4 (MQ=255)
                                                                                                                  tCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgcc                                    <  1:630848/150‑1 (MQ=255)
                                                                                                                    gtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTggcccg                                   <  2:930777/148‑6 (MQ=255)
                                                                                                                    aTCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgccgc                                  >  2:313193/1‑150 (MQ=255)
                                                                                                                     tCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCgccgcc                                 <  1:793890/150‑1 (MQ=255)
                                                                                                                     tCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCgccgcc                                 <  2:186016/150‑1 (MQ=255)
                                                                                                                      cGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgccgc                                  >  2:54547/1‑148 (MQ=255)
                                                                                                                       gTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAg                               >  2:298891/1‑150 (MQ=255)
                                                                                                                       gTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAg                               <  1:241909/150‑1 (MQ=255)
                                                                                                                       gTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAg                               <  1:416635/150‑1 (MQ=255)
                                                                                                                        tAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGc                              >  2:766182/1‑150 (MQ=255)
                                                                                                                          gacggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCccgcccgccg                            >  2:206547/5‑148 (MQ=255)
                                                                                                                              gCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcgcgt                        <  1:811339/150‑6 (MQ=255)
                                                                                                                              gCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGttttt                        >  1:111251/1‑150 (MQ=255)
                                                                                                                              gCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGttttt                        >  1:432845/1‑150 (MQ=255)
                                                                                                                               cAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcgcgtt                       <  2:422356/150‑7 (MQ=255)
                                                                                                                               cAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcgcgtt                       <  1:447218/150‑7 (MQ=255)
                                                                                                                               cAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcgcgtt                       >  1:720036/1‑144 (MQ=255)
                                                                                                                               cAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttttt                       <  2:29036/150‑1 (MQ=255)
                                                                                                                               cAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttttt                       >  2:192772/1‑150 (MQ=255)
                                                                                                                                aaTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTc                      >  2:907740/1‑150 (MQ=255)
                                                                                                                                   cAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTg                    >  1:768887/1‑149 (MQ=255)
                                                                                                                                     gTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCaa                 >  2:889280/1‑150 (MQ=255)
                                                                                                                                         aCCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCg              <  2:639901/149‑1 (MQ=255)
                                                                                                                                           ccAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCa           <  2:95319/150‑1 (MQ=255)
                                                                                                                                           ccAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCa           >  2:608368/1‑150 (MQ=255)
                                                                                                                                            cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCa           >  2:46037/1‑149 (MQ=255)
                                                                                                                                            cAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCAt          <  1:378909/150‑1 (MQ=255)
                                                                                                                                             aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa         >  1:548628/1‑150 (MQ=255)
                                                                                                                                             aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa         <  1:453091/150‑1 (MQ=255)
                                                                                                                                             aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa         >  1:869550/1‑150 (MQ=255)
                                                                                                                                             aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa         >  2:2257/1‑150 (MQ=255)
                                                                                                                                             aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa         <  1:280577/150‑1 (MQ=255)
                                                                                                                                             aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa         <  2:480205/150‑1 (MQ=255)
                                                                                                                                              gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATAc        <  1:708308/150‑1 (MQ=255)
                                                                                                                                                  cAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGAc    >  1:359996/1‑150 (MQ=255)
                                                                                                                                                   aGCGTCCCCGGCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTAGCCCATGAAGGCGTAGGTGTAGCGGTCGACAATGCCGTGGCCCGGAAGGTTAAACTCCGCCGCCAGCGTTTTTTCTGCAATCGCCCTACCGACt   <  2:801200/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTG  >  CP009273/3074330‑3074627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑