Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,602,774 G→T 6.0% P66P (CCG→CCT)  yhhQ → DUF165 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,602,7740GT 6.0% 224.2 / 3.2 66P66P (CCG→CCT) yhhQDUF165 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (24/38);  new base T (2/2);  total (26/40)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-02

TATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTATCGCCACCGCCA  >  CP009273/3602629‑3602919
                                                                                                                                                 |                                                                                                                                                 
tATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTgg                                                                                                                                               >  1:901857/1‑150 (MQ=255)
tATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTgg                                                                                                                                               >  1:779938/1‑150 (MQ=255)
    tCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGccc                                                                                                                                            >  1:382879/1‑149 (MQ=255)
           ctgGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCAt                                                                                                                                     >  1:667432/1‑149 (MQ=255)
              gTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATc                                                                                                                                 <  1:113568/150‑1 (MQ=255)
              gTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATc                                                                                                                                 <  1:455498/150‑1 (MQ=255)
                 aTCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTc                                                                                                                              <  1:162408/150‑1 (MQ=255)
                    aCCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTcgc                                                                                                                            <  1:484732/149‑1 (MQ=255)
                      cTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCTCTGGCCCGACGCATTATCTTCGCgg                                                                                                                          >  1:73751/1‑149 (MQ=255)
                      cTCCAGTAACTATCTGGTTCAGCTTCCCGTC‑CCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCTCTGGCCCGACGCATTATCTTCGCGGt                                                                                                                         >  1:73636/1‑149 (MQ=255)
                                  tCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTg                                                                                                              <  1:932975/149‑1 (MQ=255)
                                    tGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGt                                                                                                           >  1:382962/1‑150 (MQ=255)
                                      gTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGtta                                                                                                         <  1:26412/150‑1 (MQ=255)
                                         cAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGttatt                                                                                                       >  2:64485/1‑149 (MQ=255)
                                          aGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGttatta                                                                                                      <  2:49780/149‑1 (MQ=255)
                                          aGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTaa                                                                                                     >  2:677564/1‑150 (MQ=255)
                                            cTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAAtc                                                                                                   <  2:382879/150‑1 (MQ=255)
                                            cTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAAtc                                                                                                   >  2:359491/1‑150 (MQ=255)
                                              tCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAAtctc                                                                                                 >  1:759895/1‑150 (MQ=255)
                                               cccGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTcc                                                                                                >  2:750599/1‑150 (MQ=255)
                                                ccGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCt                                                                                               <  1:843900/150‑1 (MQ=255)
                                                 cGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTa                                                                                              <  1:170887/150‑1 (MQ=255)
                                                  gTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAAt                                                                                                    >  2:542595/1‑143 (MQ=255)
                                                  gTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTAc                                                                                             <  1:803150/150‑1 (MQ=255)
                                                   tctcCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAAtc                                                                                                   <  1:542595/143‑1 (MQ=255)
                                                       cATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAt                                                                                        <  1:799421/150‑1 (MQ=255)
                                                       cATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAt                                                                                        <  1:808988/150‑1 (MQ=255)
                                                           ttGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCAtctc                                                                                     <  1:86815/149‑1 (MQ=255)
                                                            tGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgt                                                                                   >  1:382897/1‑150 (MQ=255)
                                                              ggTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCtcgtcg                                                                                 <  2:877568/150‑1 (MQ=255)
                                                                        ccgccTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCtat                                                                        <  2:671250/146‑1 (MQ=255)
                                                                        ccaccTGGGGCGCGTTTATCTTTCCGTTTATTTTTCTTGCTACCGGCCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTTCGTCATCACGTCGGTATTCtct                                                                        <  2:703117/149‑3 (MQ=255)
                                                                            cTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATggg                                                                   >  1:651446/1‑150 (MQ=255)
                                                                                ggCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCTCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTcc                                                               <  2:73636/150‑1 (MQ=255)
                                                                                ggCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCTCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTcc                                                               <  2:73751/150‑1 (MQ=255)
                                                                                 gcgcGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTACGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCt                                                              <  1:281906/150‑1 (MQ=255)
                                                                                   gcgTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTgg                                                            >  1:894292/1‑150 (MQ=255)
                                                                                    cgTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTgg                                                            >  1:763727/1‑149 (MQ=255)
                                                                                     gTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTgg                                                            >  2:478549/1‑148 (MQ=255)
                                                                                          gCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGa                                                      <  1:619881/149‑1 (MQ=255)
                                                                                          gCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGAt                                                     >  1:785931/1‑150 (MQ=255)
                                                                                          gCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGAt                                                     >  1:673172/1‑150 (MQ=255)
                                                                                              tCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTc                                                   <  2:412596/148‑1 (MQ=255)
                                                                                              tCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCg                                                  <  2:905528/149‑1 (MQ=255)
                                                                                               ccGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAAGGATTCgg                                                 <  2:763727/149‑1 (MQ=255)
                                                                                                 gTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAAt                                                                                                                      >  2:440944/1‑78 (MQ=255)
                                                                                                  tttATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATg                                                                                                                     <  1:440944/78‑1 (MQ=255)
                                                                                                  tttATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCa                                             >  2:468574/1‑150 (MQ=255)
                                                                                                  tttATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCa                                             <  1:613813/150‑1 (MQ=255)
                                                                                                     aTTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTc                                          >  2:72872/1‑150 (MQ=255)
                                                                                                               cTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTc                                 <  1:359491/149‑1 (MQ=255)
                                                                                                               cTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCa                                <  2:121154/150‑1 (MQ=255)
                                                                                                                  ccGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAAc                              <  2:117782/149‑1 (MQ=255)
                                                                                                                   cGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCt                            <  2:779938/150‑1 (MQ=255)
                                                                                                                   cGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCt                            <  1:750599/150‑1 (MQ=255)
                                                                                                                      ccTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGtt                         <  1:280774/150‑1 (MQ=255)
                                                                                                                         gACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACt                                           >  1:172238/1‑129 (MQ=255)
                                                                                                                          aCCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTc                                          <  2:172238/129‑1 (MQ=255)
                                                                                                                          aCCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTg                       <  1:64485/148‑1 (MQ=255)
                                                                                                                             gTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGcc                  <  2:901857/150‑1 (MQ=255)
                                                                                                                                 gTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGCTTGTCGCCCGTa              >  1:260702/1‑150 (MQ=255)
                                                                                                                                  tATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTAt             <  1:158639/150‑1 (MQ=255)
                                                                                                                                    tttttGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTATCg           >  1:47010/1‑150 (MQ=255)
                                                                                                                                          gcgcACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTATCGCCAcc      <  2:785931/149‑1 (MQ=255)
                                                                                                                                          gcgcACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTATCGCCAcc      <  2:382962/149‑1 (MQ=255)
                                                                                                                                             cACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTATCGCCACCGCCa  <  2:461897/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TATTTCATCTGCTGGTGATCACCTCCAGTAACTATCTGGTTCAGCTTCCCGTCTCCATTTTGGGTTTCCATACCACCTGGGGCGCGTTTAGCTTTCCGTTTATTTTTCTTGCTACCGACCTGACCGTGCGTATTTTTGGCGCACCGCTGGCCCGACGCATTATCTTCGCGGTAATGATCCCTGCGTTATTAATCTCCTACGTCATCTCGTCGCTATTCTATATGGGTTCCTGGCAGGGATTCGGCGCACTCGCCCACTTCAACCTGTTTGTCGCCCGTATCGCCACCGCCA  >  CP009273/3602629‑3602919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑