Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,450 G→C 40.5% V71V (GTG→GTC) ‡ talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,4500GC40.5% 111.0 / 189.6 131V71V (GTG→GTC) ‡talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base G (36/42);  new base C (20/33);  total (56/75)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

Only 100 of 131 total aligned reads displayed.

AATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAC  >  CP009273/8306‑8597
                                                                                                                                                |                                                                                                                                                   
aaTGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcgc                                                                                                                                                >  1:766966/1‑150 (MQ=255)
aaTGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcgc                                                                                                                                                >  1:223898/1‑150 (MQ=255)
aaTGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcgc                                                                                                                                                >  1:239312/1‑150 (MQ=255)
 aTGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcgc                                                                                                                                                >  2:31137/1‑149 (MQ=255)
   gAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGcgac                                                                                                                                             >  2:792888/1‑150 (MQ=255)
    aaGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGcgacc                                                                                                                                            <  2:846336/150‑1 (MQ=255)
           aTCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAAc                                                                                                                                     >  2:675061/1‑150 (MQ=255)
               aCCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTgg                                                                                                                                  >  2:715074/1‑149 (MQ=255)
               aCCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTgg                                                                                                                                  >  2:234725/1‑149 (MQ=255)
                cctcAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGcg                                                                                                                                <  1:327994/147‑2 (MQ=255)
                 cGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAg                                                                                                                               >  1:638173/1‑150 (MQ=255)
                    aGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGtga                                                                                                                            <  1:224630/150‑3 (MQ=255)
                    aGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGtga                                                                                                                            <  1:490344/150‑3 (MQ=255)
                     ggATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTaaa                                                                                                                           >  1:701401/1‑150 (MQ=255)
                       aTGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTATCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTgaaca                                                                                                                         >  2:854196/1‑148 (MQ=255)
                          ccACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATAtt                                                                                                                       <  2:679581/149‑1 (MQ=255)
                          ccACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTg                                                                                                                      >  1:614303/1‑150 (MQ=255)
                           caccacTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCgg                                                                                                                     >  1:301853/1‑150 (MQ=255)
                            acaACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGt                                                                                                                    <  1:85638/150‑1 (MQ=255)
                             caaccaacCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTc                                                                                                                   <  2:478295/150‑1 (MQ=255)
                              aaccaaccCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCt                                                                                                                  >  2:701776/1‑150 (MQ=255)
                              aaccaaccCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCt                                                                                                                  >  2:541922/1‑150 (MQ=255)
                                 taaccCTTCCCTGATTCTCGGCACGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTgaaca                                                                                                                         >  2:547389/2‑138 (MQ=255)
                                    cccTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAAt                                                                                                            <  1:733078/150‑1 (MQ=255)
                                    cccTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAAt                                                                                                            <  1:233236/150‑1 (MQ=255)
                                    cccTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAAt                                                                                                            <  2:638173/150‑1 (MQ=255)
                                    cccTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAAt                                                                                                            <  1:120451/150‑1 (MQ=255)
                                     ccTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATc                                                                                                           <  2:13575/150‑1 (MQ=255)
                                      cTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATcc                                                                                                          <  2:388980/150‑1 (MQ=255)
                                                    aaCGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGtt                                                                                               <  1:103013/147‑1 (MQ=255)
                                                        cggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGcc                                                                                        <  2:832621/145‑1 (MQ=255)
                                                          gcgcagATTCCAGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTc                                                                                              <  2:405434/142‑1 (MQ=255)
                                                           ctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTa                                                                                     >  2:334279/3‑150 (MQ=255)
                                                           ctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTa                                                                                     >  1:872690/3‑150 (MQ=255)
                                                            tcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAAc                                                                                                    >  2:615353/2‑134 (MQ=255)
                                                            gcagATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGAcg                                                                                                                                                 >  1:46618/1‑89 (MQ=255)
                                                            gcagATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTAt                                                                                    >  2:448891/1‑150 (MQ=255)
                                                               gATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTAtctc                                                                                 >  2:495819/1‑150 (MQ=255)
                                                                   ccGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAAc                                                                              <  1:448891/149‑1 (MQ=255)
                                                                    ctgagtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCAccg                                                                            <  1:261962/145‑3 (MQ=255)
                                                                    ctgagtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCAccg                                                                            <  2:985320/145‑3 (MQ=255)
                                                                    cGGAATACCGTAGGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAGCGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACt                                                                             <  1:872342/149‑1 (MQ=255)
                                                                    cGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACt                                                                             >  2:707099/1‑149 (MQ=255)
                                                                       agtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAg                                                                         >  2:775465/3‑150 (MQ=255)
                                                                       agtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAg                                                                         >  1:136254/3‑150 (MQ=255)
                                                                        gtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGt                                                                        <  2:123966/149‑1 (MQ=255)
                                                                        aTACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGt                                                                        >  2:929977/1‑150 (MQ=255)
                                                                         tACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGtt                                                                       >  2:427874/1‑150 (MQ=255)
                                                                           ccGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGa                                                                     >  1:682758/1‑150 (MQ=255)
                                                                            cGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGAt                                                                    <  2:766966/150‑1 (MQ=255)
                                                                            cGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGAt                                                                    <  2:257518/150‑1 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAGCTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  1:142895/150‑2 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  2:566762/150‑2 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  2:648641/150‑2 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  2:674059/150‑2 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  2:935098/150‑2 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  2:112209/150‑2 (MQ=255)
                                                                            cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGac                                                                    <  1:531706/150‑2 (MQ=255)
                                                                               aaGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgca                                                                 >  2:771961/1‑149 (MQ=255)
                                                                                aGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgcg                                                                 <  1:323933/149‑1 (MQ=255)
                                                                                aGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgcg                                                                 <  1:966802/149‑1 (MQ=255)
                                                                                     attgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCt                                                            <  1:264116/149‑1 (MQ=255)
                                                                                         atgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCt                                                       <  1:60017/150‑1 (MQ=255)
                                                                                          tgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTa                                                      >  2:191245/1‑150 (MQ=255)
                                                                                            atgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTAt                                                     <  1:31137/149‑1 (MQ=255)
                                                                                            atgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATg                                                    <  2:470034/150‑1 (MQ=255)
                                                                                                  tCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGacac                                                <  1:364380/148‑1 (MQ=255)
                                                                                                  tCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACAcc                                               <  1:792888/149‑1 (MQ=255)
                                                                                                    gCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCg                                              >  1:554406/1‑148 (MQ=255)
                                                                                                         ggCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGt                                        >  1:235604/1‑149 (MQ=255)
                                                                                                           cGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCaa                                     >  2:796210/1‑150 (MQ=255)
                                                                                                              gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAtc                                   <  2:556189/144‑2 (MQ=255)
                                                                                                              gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAt                                    <  2:231371/143‑1 (MQ=255)
                                                                                                                cagcagAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGc                                 >  2:767025/1‑149 (MQ=255)
                                                                                                                    agagCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAg                            <  2:491661/150‑1 (MQ=255)
                                                                                                                     gagCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGc                           <  2:214063/150‑1 (MQ=255)
                                                                                                                       gCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGcc                          >  2:943287/1‑148 (MQ=255)
                                                                                                                        cagcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGcc                          >  2:365657/4‑147 (MQ=255)
                                                                                                                         agcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCcaag                       <  2:191909/148‑2 (MQ=255)
                                                                                                                          aCGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                      <  2:239312/150‑1 (MQ=255)
                                                                                                                           cGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACg                     >  2:293735/1‑150 (MQ=255)
                                                                                                                            gATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCg                     >  1:294147/1‑149 (MQ=255)
                                                                                                                             aTCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGcc                   >  2:455152/1‑150 (MQ=255)
                                                                                                                             aTCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgca                   <  1:605356/150‑2 (MQ=255)
                                                                                                                              tCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                  <  1:627530/150‑1 (MQ=255)
                                                                                                                                gcgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                >  2:978900/1‑150 (MQ=255)
                                                                                                                                gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                <  2:467513/150‑5 (MQ=255)
                                                                                                                                gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                >  1:480903/1‑146 (MQ=255)
                                                                                                                                gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                >  1:319030/1‑146 (MQ=255)
                                                                                                                                 cgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGAt               <  1:701776/150‑1 (MQ=255)
                                                                                                                                  gcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc              <  1:829808/150‑1 (MQ=255)
                                                                                                                                   cgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCatcatc              >  1:874030/1‑149 (MQ=255)
                                                                                                                                    gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa            <  2:790225/150‑1 (MQ=255)
                                                                                                                                    gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa            <  2:511138/150‑1 (MQ=255)
                                                                                                                                      agcagATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc          <  1:662169/150‑1 (MQ=255)
                                                                                                                                      agcagATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc          <  1:356437/150‑1 (MQ=255)
                                                                                                                                      agcagATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc          <  1:80526/150‑1 (MQ=255)
                                                                                                                                        cagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGctg        <  2:987855/150‑2 (MQ=255)
                                                                                                                                             cGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACaa   >  1:647144/1‑150 (MQ=255)
                                                                                                                                              gTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAc  <  1:204670/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAC  >  CP009273/8306‑8597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑