Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,540 T→C 53.0% L101L (CTT→CTC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,5400TC53.0% 32.4 / 203.8 134L101L (CTT→CTC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (27/36);  new base C (36/35);  total (63/71)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

ATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAG  >  CP009273/8395‑8685
                                                                                                                                                 |                                                                                                                                                 
atgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCt                                                                                                                                               <  1:60017/150‑1 (MQ=255)
 tgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTa                                                                                                                                              >  2:191245/1‑150 (MQ=255)
 tgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTa                                                                                                                                              <  1:256620/150‑1 (MQ=255)
   atgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTAt                                                                                                                                             <  1:31137/149‑1 (MQ=255)
   atgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATg                                                                                                                                            <  2:470034/150‑1 (MQ=255)
         tCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGacac                                                                                                                                        <  1:364380/148‑1 (MQ=255)
         tCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACAcc                                                                                                                                       <  1:792888/149‑1 (MQ=255)
         tCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCg                                                                                                                                      <  2:819821/150‑1 (MQ=255)
             cTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGc                                                                                                                                  >  1:870582/1‑150 (MQ=255)
                ggCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGt                                                                                                                                >  1:235604/1‑149 (MQ=255)
                  cGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCaa                                                                                                                             >  2:796210/1‑150 (MQ=255)
                     gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAtc                                                                                                                           <  2:988066/144‑2 (MQ=255)
                     gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAt                                                                                                                            <  2:231371/143‑1 (MQ=255)
                           agagCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAg                                                                                                                    <  2:491661/150‑1 (MQ=255)
                            gagCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGc                                                                                                                   <  2:214063/150‑1 (MQ=255)
                                 aCGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAAc                                                                                                              <  2:239312/150‑1 (MQ=255)
                                   gATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCg                                                                                                             >  1:294147/1‑149 (MQ=255)
                                    aTCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGcc                                                                                                           >  2:455152/1‑150 (MQ=255)
                                    aTCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgca                                                                                                           <  1:605356/150‑2 (MQ=255)
                                     tCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCt                                                                                                          <  1:627530/150‑1 (MQ=255)
                                       gcgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGa                                                                                                        >  2:978900/1‑150 (MQ=255)
                                       gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                                                                                                        >  2:10675/1‑146 (MQ=255)
                                       gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                                                                                                        <  2:467513/150‑5 (MQ=255)
                                       gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                                                                                                        >  1:480903/1‑146 (MQ=255)
                                       gcgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatca                                                                                                        <  2:948495/150‑5 (MQ=255)
                                        cgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGAt                                                                                                       >  2:414239/1‑150 (MQ=255)
                                        cgcgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAgcgcatcat                                                                                                       <  1:325982/150‑6 (MQ=255)
                                         gcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATc                                                                                                      <  1:829808/150‑1 (MQ=255)
                                          cgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCatcatc                                                                                                      >  1:874030/1‑149 (MQ=255)
                                           gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa                                                                                                    <  2:790225/150‑1 (MQ=255)
                                           gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa                                                                                                    <  2:511138/150‑1 (MQ=255)
                                             agcagATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAAc                                                                                                  <  1:662169/150‑1 (MQ=255)
                                               cagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGctg                                                                                                <  2:987855/150‑2 (MQ=255)
                                                   tCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTAc                                                                                             >  1:579709/1‑149 (MQ=255)
                                                    cGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACaa                                                                                           >  1:647144/1‑150 (MQ=255)
                                                     gTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACaa                                                                                           <  2:724669/149‑1 (MQ=255)
                                                     gTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAAc                                                                                          <  1:204670/150‑1 (MQ=255)
                                                          cgcgACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGc                                                                                     <  1:324860/150‑1 (MQ=255)
                                                            cttccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGcgg                                                                                   <  2:225863/147‑3 (MQ=255)
                                                             gaccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTgg                                                                                  >  1:322707/1‑150 (MQ=255)
                                                              tccgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCggg                                                                                  >  1:687404/2‑149 (MQ=255)
                                                                cgatAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCAt                                                                               <  1:658471/150‑1 (MQ=255)
                                                                cgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                                                                               <  2:107370/150‑1 (MQ=255)
                                                                 gacAAACTGGCAGTAAATATTGGTCTGGACATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                                                                               >  2:790979/1‑149 (MQ=255)
                                                                  acAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCAAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTAt                                                                               >  2:382544/1‑148 (MQ=255)
                                                                   taagcTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGGGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAg                                                                            >  1:13713/5‑150 (MQ=255)
                                                                       cTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAAc                                                                        >  1:15351/1‑150 (MQ=255)
                                                                          gCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACg                                                                       <  2:152594/148‑1 (MQ=255)
                                                                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa                                                                 <  1:299396/150‑1 (MQ=255)
                                                                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa                                                                 >  1:819232/1‑150 (MQ=255)
                                                                              tAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTa                                                                 <  2:614303/150‑1 (MQ=255)
                                                                               gaacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCGCGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAt                                                                >  2:112324/8‑150 (MQ=255)
                                                                                aacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAtt                                                               <  2:925892/144‑1 (MQ=255)
                                                                                aacatcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCAt                                                                <  2:319030/143‑1 (MQ=255)
                                                                                  catcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTcc                                                             >  2:668993/5‑149 (MQ=255)
                                                                                  catcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCt                                                             >  1:101974/5‑150 (MQ=255)
                                                                                   atcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCt                                                             >  2:572725/4‑149 (MQ=255)
                                                                                   atcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCt                                                             >  2:984350/4‑149 (MQ=255)
                                                                                    tcggTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGa                                                           <  2:709733/148‑1 (MQ=255)
                                                                                       gTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCa                                                        <  2:154375/150‑1 (MQ=255)
                                                                                            gAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACt                                                    <  1:895760/149‑1 (MQ=255)
                                                                                            gAAATTCTGAAACTGATCCCGGGCCATATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACt                                                    <  2:241912/149‑1 (MQ=255)
                                                                                            gAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACt                                                    <  2:870582/149‑1 (MQ=255)
                                                                                             aaaTCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTgg                                                  <  1:427874/150‑1 (MQ=255)
                                                                                              aaTTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGc                                                 >  1:395826/1‑150 (MQ=255)
                                                                                               attcTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGcc                                                >  2:716414/4‑149 (MQ=255)
                                                                                               attcTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGcc                                                >  1:549645/4‑149 (MQ=255)
                                                                                                ttcTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGcct                                               <  1:429394/148‑3 (MQ=255)
                                                                                                ttcTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGcct                                               <  1:938442/148‑3 (MQ=255)
                                                                                                   tGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTcca                                            >  1:564668/1‑148 (MQ=255)
                                                                                                   tGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTcca                                            >  1:945407/1‑148 (MQ=255)
                                                                                                         tGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTgg                                       >  1:151833/1‑149 (MQ=255)
                                                                                                           gTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGc                                      <  1:234725/148‑1 (MQ=255)
                                                                                                           atcccGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAg                                    <  1:141450/147‑1 (MQ=255)
                                                                                                               cGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGt                                 <  2:756748/149‑1 (MQ=255)
                                                                                                                    cGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGt                           >  2:313471/1‑150 (MQ=255)
                                                                                                                     gTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtg                          >  1:12935/1‑150 (MQ=255)
                                                                                                                      tATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCgcgc                         >  1:181740/1‑150 (MQ=255)
                                                                                                                      tATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgc                         >  1:553146/1‑150 (MQ=255)
                                                                                                                      tATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgc                         <  1:796210/150‑1 (MQ=255)
                                                                                                                       aTCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCgcgcc                        >  2:353990/1‑149 (MQ=255)
                                                                                                                        tctcCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCgcgcc                        <  1:943287/149‑2 (MQ=255)
                                                                                                                          tcAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgctg                       >  1:560642/1‑148 (MQ=255)
                                                                                                                           ccaccgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGcgccgct                     >  2:274696/6‑148 (MQ=255)
                                                                                                                            caccgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGcgccgctg                    <  1:209908/145‑3 (MQ=255)
                                                                                                                            caccgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGcgccgctg                    <  1:682371/145‑3 (MQ=255)
                                                                                                                              ccgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGaa                  >  1:170670/3‑149 (MQ=255)
                                                                                                                              cTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAAc                 <  2:424896/150‑1 (MQ=255)
                                                                                                                               cgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGaa                  >  1:681745/2‑148 (MQ=255)
                                                                                                                               cgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACa                <  1:120838/149‑1 (MQ=255)
                                                                                                                               cgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACa                <  1:376037/149‑1 (MQ=255)
                                                                                                                                 aaGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACa                <  1:767025/148‑1 (MQ=255)
                                                                                                                                  aGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCt             >  1:552727/1‑150 (MQ=255)
                                                                                                                                   gTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTg            <  1:715074/150‑1 (MQ=255)
                                                                                                                                       aTGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGa                   >  2:704149/1‑139 (MQ=255)
                                                                                                                                        tGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGaa                  <  1:704149/139‑1 (MQ=255)
                                                                                                                                           gcgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAaaga    <  2:579709/150‑1 (MQ=255)
                                                                                                                                           gcgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAaaga    <  1:707099/150‑1 (MQ=255)
                                                                                                                                            cgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAaagaa   <  1:191245/150‑1 (MQ=255)
                                                                                                                                             gTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAaagaag  <  2:549645/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAG  >  CP009273/8395‑8685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑