Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,654 T→C 70.8% G139G (GGT→GGC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,6540TC70.8% 375.5 / 204.6 236G139G (GGT→GGC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (39/30);  new base C (87/80);  total (126/110)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 238 total aligned reads displayed.

CGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAA  >  CP009273/8506‑8803
                                                                                                                                                    |                                                                                                                                                     
      gTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtg                                                                                                                                                >  1:12935/1‑150 (MQ=255)
       tATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgc                                                                                                                                               >  1:553146/1‑150 (MQ=255)
       tATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgc                                                                                                                                               <  1:796210/150‑1 (MQ=255)
        aTCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCgcgcc                                                                                                                                              >  2:353990/1‑149 (MQ=255)
           tcAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGtgctg                                                                                                                                             >  1:560642/1‑148 (MQ=255)
            ccaccgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGcgccgct                                                                                                                                           >  2:274696/6‑148 (MQ=255)
               cTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAAc                                                                                                                                       <  1:978900/150‑1 (MQ=255)
                cgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGaa                                                                                                                                        >  1:681745/2‑148 (MQ=255)
                cgAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACa                                                                                                                                      <  1:376037/149‑1 (MQ=255)
                    gTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTg                                                                                                                                  <  1:715074/150‑1 (MQ=255)
                        aTGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGa                                                                                                                                         >  2:704149/1‑139 (MQ=255)
                           cgcgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGaaa                                                                                                                              >  1:663844/1‑147 (MQ=255)
                             cgTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAaagaa                                                                                                                         <  1:191245/150‑1 (MQ=255)
                                  ctCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCAt                                                                                                                    >  2:93557/2‑150 (MQ=255)
                                   tCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGTCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGc                                                                                                                      <  2:956759/147‑1 (MQ=255)
                                   tCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCa                                                                                                                     <  1:831789/148‑1 (MQ=255)
                                     cTAAGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCAtta                                                                                                                  <  2:395826/149‑3 (MQ=255)
                                       aTGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACt                                                                                                               >  1:692024/1‑150 (MQ=255)
                                         gACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTg                                                                                                              >  2:726019/1‑149 (MQ=255)
                                         gACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTg                                                                                                              >  1:737509/1‑149 (MQ=255)
                                          acacCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTa                                                                                                            <  1:110106/150‑1 (MQ=255)
                                          acacCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTa                                                                                                            <  1:460794/150‑1 (MQ=255)
                                            acCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAAc                                                                                                          <  2:43231/150‑1 (MQ=255)
                                            acCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAAc                                                                                                          <  2:923437/150‑1 (MQ=255)
                                             ccGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAAcc                                                                                                         <  2:388450/150‑1 (MQ=255)
                                             ccGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAAcc                                                                                                         <  2:322707/150‑1 (MQ=255)
                                             ccGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAAcc                                                                                                         <  2:12935/150‑1 (MQ=255)
                                              cGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCt                                                                                                        <  1:902229/150‑1 (MQ=255)
                                                 aGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGAc                                                                                                     <  1:404012/150‑1 (MQ=255)
                                                   cGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGAcgc                                                                                                   <  1:101803/150‑3 (MQ=255)
                                                   cGTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGAcgc                                                                                                   >  1:300901/1‑148 (MQ=255)
                                                    gTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGct                                                                                                  >  1:570298/1‑150 (MQ=255)
                                                    gTCTATCGCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGct                                                                                                  <  1:112324/150‑1 (MQ=255)
                                                       aaTTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCctgct                                                                                               >  1:157216/1‑150 (MQ=255)
                                                         tcgCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGctgctg                                                                                              <  2:521670/147‑1 (MQ=255)
                                                          cgCGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGtt                                                                                            >  2:231087/2‑150 (MQ=255)
                                                            cGAAAGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTtct                                                                                          <  1:39255/150‑1 (MQ=255)
                                                            cGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTtct                                                                                          <  2:151833/150‑1 (MQ=255)
                                                            cGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTtct                                                                                          <  1:929977/150‑1 (MQ=255)
                                                             gAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTtctc                                                                                         >  2:549671/1‑150 (MQ=255)
                                                                aGCCAAGCGCATCATCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCtt                                                                                      <  2:415496/150‑1 (MQ=255)
                                                                aGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCtt                                                                                      <  2:554406/150‑1 (MQ=255)
                                                                   aaaaCGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCCCCTTCGc                                                                                   >  2:167985/1‑150 (MQ=255)
                                                                    aaaCGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCt                                                                                  >  1:93762/1‑150 (MQ=255)
                                                                      gcgcatcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCt                                                                                  >  2:674542/8‑148 (MQ=255)
                                                                       cgcatcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAg                                                                               >  1:13626/7‑150 (MQ=255)
                                                                       cGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAg                                                                               >  2:758561/1‑150 (MQ=255)
                                                                        gcatcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAgg                                                                              <  1:763688/145‑1 (MQ=255)
                                                                          cTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCt                                                                            <  1:541922/150‑1 (MQ=255)
                                                                          atcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCt                                                                            <  1:320873/147‑1 (MQ=255)
                                                                          atcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCt                                                                            >  1:218176/4‑150 (MQ=255)
                                                                          atcaTCAAGCTGTACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCt                                                                            >  1:632906/4‑150 (MQ=255)
                                                                                  gctgtACAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTtg                                                                    >  2:548585/5‑150 (MQ=255)
                                                                                        cAACGACGCGGGCATCAGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTgcgccg                                                               >  2:774050/1‑147 (MQ=255)
                                                                                                   gcatcaGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCgtct                                                   >  2:658260/6‑148 (MQ=255)
                                                                                                     atcaGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                 >  2:161051/4‑150 (MQ=255)
                                                                                                     atcaGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTc                                                 >  2:689551/4‑150 (MQ=255)
                                                                                                        aGCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCt                                               <  1:232505/149‑1 (MQ=255)
                                                                                                         gCAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGa                                             <  1:405718/150‑1 (MQ=255)
                                                                                                          cAACGATCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGAt                                            >  2:304496/1‑150 (MQ=255)
                                                                                                              gATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCg                                        >  2:574502/1‑150 (MQ=255)
                                                                                                               aTCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGc                                       <  2:835260/150‑1 (MQ=255)
                                                                                                                tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                      <  2:26426/150‑1 (MQ=255)
                                                                                                                tCGCATTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGcc                                      >  1:587848/1‑150 (MQ=255)
                                                                                                                 cgcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCg                                     >  2:843451/4‑150 (MQ=255)
                                                                                                                  gcaTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCg                                     <  2:753841/147‑1 (MQ=255)
                                                                                                                  gTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCg                                     <  2:446219/149‑1 (MQ=255)
                                                                                                                  gTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGt                                    >  1:725580/1‑150 (MQ=255)
                                                                                                                    aTTCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGttc                                  <  1:257178/150‑2 (MQ=255)
                                                                                                                     ttCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGttt                                  <  2:663844/149‑1 (MQ=255)
                                                                                                                     ttCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGttcg                                 >  2:490149/1‑148 (MQ=255)
                                                                                                                     ttCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGttcg                                 <  2:564668/150‑3 (MQ=255)
                                                                                                                      tCTGATCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTtcgt                                >  2:509595/1‑150 (MQ=255)
                                                                                                                           tCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGcc                           <  2:902708/150‑1 (MQ=255)
                                                                                                                           tCAAACTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGcc                           <  1:495758/150‑1 (MQ=255)
                                                                                                                             aaaCTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCCCAGGCCCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTCGGCCg                          >  1:177520/1‑149 (MQ=255)
                                                                                                                                cTGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTAtc                      >  1:34417/1‑149 (MQ=255)
                                                                                                                                 tGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTc                     >  2:643372/1‑150 (MQ=255)
                                                                                                                                 tGGCCTCCACCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTAtcc                     >  2:812848/1‑148 (MQ=255)
                                                                                                                                   gcctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtc                   <  1:510435/144‑2 (MQ=255)
                                                                                                                                   gcctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtc                   <  1:441445/144‑2 (MQ=255)
                                                                                                                                    cctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtc                   >  2:98028/6‑148 (MQ=255)
                                                                                                                                    cctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCtc                   >  1:413687/6‑148 (MQ=255)
                                                                                                                                     ctccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGa                 >  1:483897/5‑150 (MQ=255)
                                                                                                                                      tccaCCTGGCAGGGCATTTGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGAc                >  2:608718/4‑150 (MQ=255)
                                                                                                                                       ccaCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACt               >  1:449347/3‑150 (MQ=255)
                                                                                                                                        tACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTg              >  1:271913/1‑150 (MQ=255)
                                                                                                                                        caCCTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTg              <  1:670215/149‑1 (MQ=255)
                                                                                                                                          ccTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTgg             <  2:629677/149‑1 (MQ=255)
                                                                                                                                          ccTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTg              <  2:737509/148‑1 (MQ=255)
                                                                                                                                          ccTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTgg             >  2:537955/1‑149 (MQ=255)
                                                                                                                                           cTGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTa           >  2:10781/1‑150 (MQ=255)
                                                                                                                                            tGGCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTa           >  1:609946/1‑149 (MQ=255)
                                                                                                                                             ggCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTAc          >  1:386805/1‑149 (MQ=255)
                                                                                                                                              gCAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACa         <  1:689551/149‑1 (MQ=255)
                                                                                                                                               cAGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACa         >  2:476831/1‑148 (MQ=255)
                                                                                                                                                aGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAg      <  1:284679/150‑1 (MQ=255)
                                                                                                                                                aGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAg      >  2:204827/1‑150 (MQ=255)
                                                                                                                                                aGGGCATTCGCGCCGCTGAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAg      >  1:771754/1‑150 (MQ=255)
                                                                                                                                                    tATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGaa  <  2:189461/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGTATTAGCAACGATCGTATTCTGATCAAACTGGCTTCTACCTGGCAGGGTATCCGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAA  >  CP009273/8506‑8803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑