BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,480	Δ1 bp	100%	coding (1/963 nt)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,480	0	A	.	100.0%	859.0 / NA	174	coding (1/963 nt)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (86/88); total (86/88)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

TAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGA  >  CP009273/4097336‑4097622

taAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTt                                                                                                                                          <  2:977104‑M1/150‑2 (MQ=255)

 aaaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtc                                                                                                                                         >  1:973029‑M1/1‑148 (MQ=255)

  aaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcg                                                                                                                                        >  1:404701‑M1/1‑147 (MQ=255)

    aTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcgg                                                                                                                                       >  2:289354‑M1/1‑145 (MQ=255)

    aTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcgg                                                                                                                                       >  1:591983‑M1/1‑145 (MQ=255)

      aCCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaa                                                                                                                                     >  2:96325‑M1/1‑143 (MQ=255)

         gCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaag                                                                                                                                 <  2:860281‑M1/150‑11 (MQ=255)

          ccATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  2:707904‑M1/1‑139 (MQ=255)

          ccATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                <  1:580010‑M1/150‑12 (MQ=255)

            aTTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCATTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  1:654206‑M1/1‑137 (MQ=255)

            aTTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  1:722372‑M1/1‑137 (MQ=255)

            aTTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  1:494122‑M1/1‑137 (MQ=255)

             tttGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcag                                                                                                                             <  1:289354‑M1/150‑15 (MQ=255)

                ggCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagatt                                                                                                                          >  2:216622‑M1/1‑133 (MQ=255)

                 gCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattc                                                                                                                         >  2:521903‑M1/1‑132 (MQ=255)

                 gCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagatt                                                                                                                          <  2:626084‑M1/149‑18 (MQ=255)

                  cctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcc                                                                                                                        >  2:46711‑M1/1‑131 (MQ=255)

                  cctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcc                                                                                                                        <  1:1030299‑M1/150‑20 (MQ=255)

                      acctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattccttta                                                                                                                    >  1:220628‑M1/1‑127 (MQ=255)

                        ctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttacc                                                                                                                  >  1:501816‑M1/1‑125 (MQ=255)

                         tgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccc                                                                                                                 >  2:918430‑M1/1‑124 (MQ=255)

                           aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                <  2:131994‑M1/149‑28 (MQ=255)

                           aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                <  2:259704‑M1/149‑28 (MQ=255)

                                aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                          <  2:391806‑M1/150‑34 (MQ=255)

                                 ttCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                          >  1:425058‑M1/1‑116 (MQ=255)

                                    agcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatg                                                                                                      <  1:333001‑M1/150‑38 (MQ=255)

                                    agcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                       <  1:47422‑M1/149‑37 (MQ=255)

                                    agcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                       >  2:884004‑M1/1‑113 (MQ=255)

                                    agcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                       >  1:355973‑M1/1‑113 (MQ=255)

                                    agcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                       <  2:525926‑M1/149‑37 (MQ=255)

                                     gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                     >  1:867407‑M1/1‑112 (MQ=255)

                                     gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                     <  1:824953‑M1/150‑39 (MQ=255)

                                     gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                     <  1:386382‑M1/150‑39 (MQ=255)

                                       agGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgaca                                                                                                   <  1:918689‑M1/150‑41 (MQ=255)

                                        ggAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                     >  1:575631‑M1/1‑109 (MQ=255)

                                          aaGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacag                                                                                                  >  2:679194‑M1/1‑107 (MQ=255)

                                                 tGTTATACTATTTGCACATGCGTTGGATCACTTAGATGTGTAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGTTAGTC‑TGATTgcggataaaggcagaattctttaccctgaaaccgacgacagaagcaaaaa                                                                                         >  2:146290‑M1/1‑100 (MQ=255)

                                                  gTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaat                                                                                        >  1:381766‑M1/1‑99 (MQ=255)

                                                    tataCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgc                                                                                      >  1:129295‑M1/1‑97 (MQ=255)

                                                      taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcct                                                                                    >  2:407924‑M1/1‑95 (MQ=255)

                                                          aTTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatg                                                                                >  2:778172‑M1/1‑91 (MQ=255)

                                                           tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgc                                                                               >  2:279293‑M1/1‑90 (MQ=255)

                                                           tttGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgc                                                                               <  1:320034‑M1/150‑61 (MQ=255)

                                                              gCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgc                                                                             <  1:500248‑M1/149‑63 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                           <  2:425058‑M1/150‑65 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                           <  1:895969‑M1/150‑65 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                           <  1:96325‑M1/150‑65 (MQ=255)

                                                               cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgct                                                                            <  1:123454‑M1/149‑64 (MQ=255)

                                                                acaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttc                                                                          <  2:209814‑M1/150‑66 (MQ=255)

                                                                 caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcg                                                                         <  2:436197‑M1/150‑67 (MQ=255)

                                                                      gTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctga                                                                                  >  2:91018‑M1/1‑79 (MQ=255)

                                                                       ttGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatc                                                                   <  1:321617‑M1/150‑73 (MQ=255)

                                                                         ggATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcag                                                                 >  1:470338‑M1/1‑76 (MQ=255)

                                                                         ggATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatca                                                                  <  2:381766‑M1/149‑74 (MQ=255)

                                                                             cACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcc                                                              >  1:1011512‑M1/1‑72 (MQ=255)

                                                                              aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                           >  2:243557‑M1/1‑71 (MQ=255)

                                                                               cTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttc                                                                          <  1:243557‑M1/135‑66 (MQ=255)

                                                                                ttCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctaca                                                          >  2:45616‑M1/1‑69 (MQ=255)

                                                                                  cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacat                                                         <  2:489376‑M1/149‑83 (MQ=255)

                                                                                  cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacat                                                         <  2:939822‑M1/149‑83 (MQ=255)

                                                                                    aTGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaa                                                      <  1:273331‑M1/150‑86 (MQ=255)

                                                                                    aTGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaa                                                      >  1:817968‑M1/1‑65 (MQ=255)

                                                                                     tgtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaagccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaat                                                     <  1:447574‑M1/150‑87 (MQ=255)

                                                                                      gtgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaatt                                                    >  2:535416‑M1/1‑63 (MQ=255)

                                                                                       tgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattc                                                   >  1:843008‑M1/1‑62 (MQ=255)

                                                                                       tgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaatt                                                    <  2:385669‑M1/149‑88 (MQ=255)

                                                                                         cAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                 <  2:728851‑M1/150‑91 (MQ=255)

                                                                                         cAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                 >  1:520915‑M1/1‑60 (MQ=255)

                                                                                            gaagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaa                                              <  2:701854‑M1/150‑94 (MQ=255)

                                                                                            gaagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaa                                              <  2:829735‑M1/150‑94 (MQ=255)

                                                                                             aagaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaat                                             >  2:250446‑M1/1‑56 (MQ=255)

                                                                                              agaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatt                                            >  2:475723‑M1/1‑55 (MQ=255)

                                                                                                    ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttatt                                        <  1:706563‑M1/148‑100 (MQ=255)

                                                                                                    ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttatt                                        <  1:939832‑M1/148‑100 (MQ=255)

                                                                                                     cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaa                                     >  2:563498‑M1/1‑48 (MQ=255)

                                                                                                     cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaa                                     >  1:107201‑M1/1‑48 (MQ=255)

                                                                                                      ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                    >  1:938184‑M1/1‑47 (MQ=255)

                                                                                                        cAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaattt                                  >  1:547845‑M1/1‑45 (MQ=255)

                                                                                                        cAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaattt                                  <  2:843008‑M1/150‑106 (MQ=255)

                                                                                                         aaCAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttg                                 <  1:84851‑M1/150‑107 (MQ=255)

                                                                                                         aaCAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttg                                 >  1:382314‑M1/1‑44 (MQ=255)

                                                                                                           cAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgca                               <  2:329713‑M1/150‑109 (MQ=255)

                                                                                                             gATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaa                             <  2:287201‑M1/150‑111 (MQ=255)

                                                                                                               tttCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaact                           >  1:744978‑M1/1‑38 (MQ=255)

                                                                                                                 tCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactt                          <  2:654206‑M1/149‑114 (MQ=255)

                                                                                                                  cATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttt                         <  1:408874‑M1/149‑115 (MQ=255)

                                                                                                                   aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttg                       <  1:247176‑M1/150‑117 (MQ=255)

                                                                                                                   aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactttt                        <  2:487980‑M1/149‑116 (MQ=255)

                                                                                                                   aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactttt                        <  1:475723‑M1/149‑116 (MQ=255)

                                                                                                                    ttttGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttg                       >  2:225525‑M1/1‑33 (MQ=255)

                                                                                                                          aTTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccg                <  1:763863‑M1/150‑124 (MQ=255)

                                                                                                                          aTTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccg                >  1:994894‑M1/1‑27 (MQ=255)

                                                                                                                            tCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccgga              >  1:605075‑M1/1‑25 (MQ=255)

                                                                                                                                aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          <  1:162121‑M1/150‑130 (MQ=255)

                                                                                                                                 aGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataagg         >  1:624958‑M1/1‑20 (MQ=255)

                                                                                                                                 aGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataagg         <  2:102649‑M1/150‑131 (MQ=255)

                                                                                                                                  gTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggc        <  2:501816‑M1/150‑132 (MQ=255)

                                                                                                                                       gagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcg   >  1:880462‑M1/1‑14 (MQ=255)

                                                                                                                                       gagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcg   >  1:909851‑M1/1‑14 (MQ=255)

                                                                                                                                        agGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgc  <  1:680915‑M1/150‑138 (MQ=255)

TAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGA  >  CP009273/4097336‑4097622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`