Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 9,029 T→A 72.8% S264S (TCT→TCA) ‡ talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092739,0290TA72.8% 430.0 / 184.3 216S264S (TCT→TCA) ‡talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base A (73/84);  total (103/113)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

GAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCG  >  CP009273/8880‑9177
                                                                                                                                                     |                                                                                                                                                    
gagCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCt                                                                                                                                                      >  1:146555/1‑150 (MQ=255)
  gCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTa                                                                                                                                                    <  2:222411/150‑1 (MQ=255)
     cGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTacac                                                                                                                                                 <  2:880374/150‑1 (MQ=255)
      ggTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                                                                                <  1:516385/150‑1 (MQ=255)
             aaaCCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAg                                                                                                                                         >  2:664201/1‑150 (MQ=255)
              aaCCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGt                                                                                                                                        <  1:457857/150‑1 (MQ=255)
              aaCCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGt                                                                                                                                        <  1:876945/150‑1 (MQ=255)
              aaCCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGt                                                                                                                                        >  1:262545/1‑150 (MQ=255)
                 cgtcgtGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGa                                                                                                                                      >  1:787908/1‑149 (MQ=255)
                  gtcgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGa                                                                                                                                      >  2:1052501/7‑148 (MQ=255)
                   tGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                                                                                   <  1:378662/150‑1 (MQ=255)
                    cgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgc                                                                                                                                  >  1:40592/5‑150 (MQ=255)
                     gtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgcg                                                                                                                                 >  2:454364/4‑150 (MQ=255)
                     gtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgcg                                                                                                                                 <  1:363648/147‑1 (MQ=255)
                     gTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAgc                                                                                                                                  <  1:636064/149‑1 (MQ=255)
                     gTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAgc                                                                                                                                  <  2:977384/149‑1 (MQ=255)
                        aTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGt                                                                                                                              >  1:534967/1‑150 (MQ=255)
                         tGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTc                                                                                                                             >  1:642752/1‑150 (MQ=255)
                            gcgcAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCgg                                                                                                                          <  2:258152/150‑1 (MQ=255)
                            gcgcAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCg                                                                                                                           >  2:755508/1‑149 (MQ=255)
                             cgcAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCgga                                                                                                                         <  2:546003/150‑2 (MQ=255)
                              gcAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCggag                                                                                                                        <  1:955436/150‑3 (MQ=255)
                                aaGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGagcg                                                                                                                      >  1:800044/1‑150 (MQ=255)
                                aaGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGagcg                                                                                                                      >  2:382658/1‑150 (MQ=255)
                                aaGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                                                                                      >  2:440958/1‑150 (MQ=255)
                                 aGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGt                                                                                                                     <  2:211958/150‑1 (MQ=255)
                                   cTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTAt                                                                                                                   >  1:437069/1‑150 (MQ=255)
                                    ttCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                                                                                   >  1:383753/1‑149 (MQ=255)
                                    ttCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                                                                                   >  1:219649/1‑149 (MQ=255)
                                     tCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtta                                                                                                                 >  1:582195/1‑148 (MQ=255)
                                     tCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtta                                                                                                                 >  2:579623/1‑148 (MQ=255)
                                      ccGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCa                                                                                                                 <  1:341686/149‑1 (MQ=255)
                                      ccGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCa                                                                                                                 <  1:780784/149‑1 (MQ=255)
                                       cGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAc                                                                                                                <  2:901184/149‑1 (MQ=255)
                                       cGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAc                                                                                                                <  2:915780/149‑1 (MQ=255)
                                       cGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAc                                                                                                                <  1:820379/149‑1 (MQ=255)
                                        gTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAcc                                                                                                               >  2:342122/1‑148 (MQ=255)
                                        gTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAcc                                                                                                               >  2:330833/1‑148 (MQ=255)
                                        gTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAcc                                                                                                               >  1:769003/1‑148 (MQ=255)
                                        gTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTAcc                                                                                                               >  1:327043/1‑148 (MQ=255)
                                            cgttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATc                                                                                                          >  1:193998/5‑150 (MQ=255)
                                            cgttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATc                                                                                                          <  1:118716/146‑1 (MQ=255)
                                             gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                                                                         <  2:78827/147‑2 (MQ=255)
                                                ggCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAg                                                                                                      <  1:890708/150‑1 (MQ=255)
                                                ggCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAg                                                                                                      >  2:107846/1‑150 (MQ=255)
                                                   gAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTc                                                                                                   >  1:723074/1‑150 (MQ=255)
                                                           ggAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTgg                                                                                             >  2:367248/1‑148 (MQ=255)
                                                                   ctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAAc                                                                                    >  1:55093/3‑149 (MQ=255)
                                                                      gCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAgg                                                                                <  1:425011/150‑1 (MQ=255)
                                                                        tgtgACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTc                                                                                                   >  2:704598/4‑129 (MQ=255)
                                                                          tgACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGAt                                                                              <  1:713406/147‑1 (MQ=255)
                                                                          tgACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGAt                                                                              <  2:284707/147‑1 (MQ=255)
                                                                          cGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATcc                                                                            <  1:988675/150‑1 (MQ=255)
                                                                           gACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATcc                                                                            >  2:888051/1‑149 (MQ=255)
                                                                           gACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGCCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATcc                                                                            >  1:489420/1‑149 (MQ=255)
                                                                           gACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATcc                                                                            >  1:1053302/1‑149 (MQ=255)
                                                                            aCCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATccga                                                                          <  2:467786/150‑3 (MQ=255)
                                                                            aCCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATccga                                                                          <  2:434016/150‑3 (MQ=255)
                                                                                  tGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGATAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagcata                                                                                      <  1:9982/132‑3 (MQ=255)
                                                                                       aTCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGa                                                                >  1:246383/1‑149 (MQ=255)
                                                                                        tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                              <  1:1006146/150‑3 (MQ=255)
                                                                                           ccccGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAAc                                                           >  2:1195/3‑150 (MQ=255)
                                                                                             ccGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTg                                                         <  1:603592/150‑1 (MQ=255)
                                                                                               ggCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGc                                                       <  2:338097/150‑1 (MQ=255)
                                                                                                   ctgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGaa                                                   <  1:418325/150‑1 (MQ=255)
                                                                                                      ctgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAg                                                  <  2:196451/148‑1 (MQ=255)
                                                                                                        gAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTAt                                              >  2:740874/1‑150 (MQ=255)
                                                                                                         aaaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTAt                                              <  2:728679/149‑1 (MQ=255)
                                                                                                          aaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATcc                                            <  2:951520/150‑1 (MQ=255)
                                                                                                            gagCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGt                                          <  2:50674/150‑1 (MQ=255)
                                                                                                             agCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGt                                          >  2:18311/1‑149 (MQ=255)
                                                                                                             agCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTa                                         <  1:68801/150‑1 (MQ=255)
                                                                                                             agCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTa                                         <  2:59465/150‑1 (MQ=255)
                                                                                                              gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                        <  2:68013/150‑1 (MQ=255)
                                                                                                              gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                        <  1:440765/150‑1 (MQ=255)
                                                                                                              gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                        <  2:40592/150‑1 (MQ=255)
                                                                                                              gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTAAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                        <  1:931304/150‑1 (MQ=255)
                                                                                                                  tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                    <  1:278696/144‑1 (MQ=255)
                                                                                                                  tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGt                                      <  2:1021602/142‑1 (MQ=255)
                                                                                                                    cgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                  <  1:662125/146‑1 (MQ=255)
                                                                                                                     gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcg                                 <  2:849995/147‑2 (MQ=255)
                                                                                                                     gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                  >  2:1028652/4‑149 (MQ=255)
                                                                                                                     gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                  >  1:290649/4‑149 (MQ=255)
                                                                                                                     gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                  >  1:355343/4‑149 (MQ=255)
                                                                                                                      aaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcga                                >  2:622627/3‑148 (MQ=255)
                                                                                                                      aaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcga                                >  2:533104/3‑148 (MQ=255)
                                                                                                                       aagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAt                               >  1:899522/2‑150 (MQ=255)
                                                                                                                       aagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAt                               <  1:65180/149‑1 (MQ=255)
                                                                                                                        agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                              >  1:908094/1‑149 (MQ=255)
                                                                                                                        agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                              <  2:818553/150‑2 (MQ=255)
                                                                                                                        agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                              <  2:826824/150‑2 (MQ=255)
                                                                                                                         gCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtcg                             <  2:927914/150‑3 (MQ=255)
                                                                                                                          cGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGCGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGa                            >  2:464535/1‑150 (MQ=255)
                                                                                                                           gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                           <  1:888051/150‑1 (MQ=255)
                                                                                                                             aGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCa                         <  1:925836/150‑1 (MQ=255)
                                                                                                                                          cGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGa             >  1:844462/1‑149 (MQ=255)
                                                                                                                                              aaCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaaa        >  1:165502/1‑150 (MQ=255)
                                                                                                                                                  gTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGAt    >  2:242187/1‑150 (MQ=255)
                                                                                                                                                   tCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGAt    <  1:523049/149‑1 (MQ=255)
                                                                                                                                                    cTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCg  >  2:26281/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCG  >  CP009273/8880‑9177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑