Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 9,110 A→G 70.4% A291A (GCA→GCG)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092739,1100AG70.4% 285.6 / 157.6 189A291A (GCA→GCG) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base A (26/30);  new base G (55/78);  total (81/108)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

Only 100 of 191 total aligned reads displayed.

TGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGC  >  CP009273/8962‑9259
                                                                                                                                                    |                                                                                                                                                     
   ccATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAg                                                                                                                                                   <  1:821926/150‑1 (MQ=255)
     aTCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGa                                                                                                                                                  >  2:580839/1‑149 (MQ=255)
     aTCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGa                                                                                                                                                  >  2:537789/1‑149 (MQ=255)
      tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                                                                                                                <  1:1006146/150‑3 (MQ=255)
      tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                                                                                                                <  2:1020731/150‑3 (MQ=255)
      tCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGaca                                                                                                                                                <  1:259467/150‑3 (MQ=255)
        gCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATaaa                                                                                                                                              <  2:162708/150‑1 (MQ=255)
         ccccGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAAc                                                                                                                                             >  2:1195/3‑150 (MQ=255)
          aCCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACt                                                                                                                                            >  1:358448/1‑150 (MQ=255)
             ggCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGc                                                                                                                                         <  2:338097/150‑1 (MQ=255)
              gcgctgctgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCg                                                                                                                                        <  1:439181/147‑1 (MQ=255)
                 ctgctgAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGaa                                                                                                                                     <  1:418325/150‑1 (MQ=255)
                    ctgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAg                                                                                                                                    <  2:196451/148‑1 (MQ=255)
                    ctgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAg                                                                                                                                    <  1:896612/148‑1 (MQ=255)
                     tgAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTa                                                                                                                                 >  2:251708/1‑150 (MQ=255)
                      gAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTAt                                                                                                                                >  2:740874/1‑150 (MQ=255)
                       aaaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTAt                                                                                                                                <  1:872811/149‑1 (MQ=255)
                       aaaGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTAt                                                                                                                                <  1:440958/149‑1 (MQ=255)
                        aaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATcc                                                                                                                              <  2:391307/150‑1 (MQ=255)
                        aaGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATcc                                                                                                                              <  2:951520/150‑1 (MQ=255)
                           agCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTa                                                                                                                           <  2:59465/150‑1 (MQ=255)
                            gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                                                                                                          <  2:68013/150‑1 (MQ=255)
                            gCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTAAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTaa                                                                                                                          <  1:931304/150‑1 (MQ=255)
                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                                                                                                      <  1:800553/144‑1 (MQ=255)
                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGttt                                                                                                                      <  1:278696/144‑1 (MQ=255)
                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGtt                                                                                                                       <  2:1040706/143‑1 (MQ=255)
                                tccgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGt                                                                                                                        <  1:649605/142‑1 (MQ=255)
                                  cgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTg                                                                                                                     <  1:209411/145‑1 (MQ=255)
                                  cgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTg                                                                                                                     <  1:493540/145‑1 (MQ=255)
                                  cgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                                                                    >  2:919556/5‑150 (MQ=255)
                                  cgaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                                                                    <  1:662125/146‑1 (MQ=255)
                                   gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcg                                                                                                                   <  2:781273/147‑2 (MQ=255)
                                   gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                                                                    >  1:290649/4‑149 (MQ=255)
                                   gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                                                                    >  1:354755/4‑149 (MQ=255)
                                   gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                                                                    >  2:1028652/4‑149 (MQ=255)
                                   gaaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGc                                                                                                                    >  1:721461/4‑149 (MQ=255)
                                    aaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcga                                                                                                                  >  2:533104/3‑148 (MQ=255)
                                    aaagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGcga                                                                                                                  >  2:622627/3‑148 (MQ=255)
                                     aagCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAt                                                                                                                 <  2:502607/149‑1 (MQ=255)
                                      agCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTAtt                                                                                                                <  1:740874/150‑1 (MQ=255)
                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                                                <  1:579623/150‑2 (MQ=255)
                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                                                <  2:751022/150‑2 (MQ=255)
                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                                                <  2:730973/150‑2 (MQ=255)
                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                                                <  2:826824/150‑2 (MQ=255)
                                      agCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtc                                                                                                                >  1:908094/1‑149 (MQ=255)
                                       gCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGAtcg                                                                                                               <  1:408202/150‑3 (MQ=255)
                                         gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                                                                                                             <  1:888051/150‑1 (MQ=255)
                                         gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                                                                                                             >  2:90439/1‑150 (MQ=255)
                                         gAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGAc                                                                                                             >  2:8203/1‑150 (MQ=255)
                                                     gAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTCGCTATTGACCAGGAAAAACTg                                                                                                 >  2:270645/1‑150 (MQ=255)
                                                        cGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGa                                                                                               >  1:844462/1‑149 (MQ=255)
                                                            aaCTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGaaaaaa                                                                                          >  1:165502/1‑150 (MQ=255)
                                                              cTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATg                                                                                        <  2:437069/150‑1 (MQ=255)
                                                                ggtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGAt                                                                                      >  2:85756/8‑150 (MQ=255)
                                                                gTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGAt                                                                                      >  2:242187/1‑150 (MQ=255)
                                                                 tCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGAt                                                                                      <  1:523049/149‑1 (MQ=255)
                                                                 gtatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                                                                     <  1:933173/144‑1 (MQ=255)
                                                                  tatatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATc                                                                                     >  2:314140/6‑149 (MQ=255)
                                                                  cTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCg                                                                                    >  2:26281/1‑150 (MQ=255)
                                                                    tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                                                                  >  1:374752/4‑148 (MQ=255)
                                                                    tatacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                                                                  <  1:755508/147‑3 (MQ=255)
                                                                     atacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCgac                                                                                  >  1:115541/3‑147 (MQ=255)
                                                                      tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                 <  2:195129/148‑1 (MQ=255)
                                                                      tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                 <  1:526976/148‑1 (MQ=255)
                                                                      tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                 <  2:619110/148‑1 (MQ=255)
                                                                      tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                 <  1:60732/148‑1 (MQ=255)
                                                                      tacCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACg                                                                                 <  2:422058/148‑1 (MQ=255)
                                                                      cacCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCg                                                                                 <  2:489420/149‑1 (MQ=255)
                                                                       acCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaa                                                                               >  1:177146/1‑149 (MQ=255)
                                                                        ccGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaac                                                                              >  2:957189/1‑148 (MQ=255)
                                                                        ccGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATcgacgaac                                                                              <  2:711169/150‑3 (MQ=255)
                                                                        ccGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATc                                                                              <  1:128647/150‑1 (MQ=255)
                                                                         cGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAct                                                                             <  2:387558/150‑1 (MQ=255)
                                                                         cGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAAct                                                                             >  2:36239/1‑150 (MQ=255)
                                                                           gtgAAGTGAAAGCGCGTCCGGTGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgc                                                                           >  1:833042/3‑150 (MQ=255)
                                                                            tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                                                          >  1:789408/2‑150 (MQ=255)
                                                                            tgAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAActgct                                                                          <  1:469786/149‑1 (MQ=255)
                                                                              aaGTGAAAGCGCGTCCGGAGCGTATTGCCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                        <  1:213586/150‑1 (MQ=255)
                                                                                gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                        >  2:188160/1‑148 (MQ=255)
                                                                                gTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                        >  2:714061/1‑148 (MQ=255)
                                                                                 tGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGt                                                                        >  1:819092/1‑147 (MQ=255)
                                                                                  gAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAAt                                                                     >  1:55611/1‑149 (MQ=255)
                                                                                  gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                                                                      >  1:1061672/1‑148 (MQ=255)
                                                                                  gAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                                                                      >  2:402888/1‑148 (MQ=255)
                                                                                   aaaGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaa                                                                      >  1:654531/1‑147 (MQ=255)
                                                                                     aGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaa                                                                 <  1:702288/150‑6 (MQ=255)
                                                                                      gcgcGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTAGACAAACTGGCGGAAGGTATCCGTAAGTTTGCGATCGACCAGGAAAAACTGGAAAAAATGATCGACGAACTGCTGTaagagaat                                                                <  2:22029/150‑7 (MQ=255)
                                                                                        gcgTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCtt                                                              <  2:657375/150‑1 (MQ=255)
                                                                                             cGGCGCGTATCACTGAGTCCAAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAG‑TTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTg                                                        <  2:888443/150‑1 (MQ=255)
                                                                                                       cACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAg                                               >  2:841410/1‑150 (MQ=255)
                                                                                                        aCTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAg                                               <  2:818006/149‑1 (MQ=255)
                                                                                                                    ttCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTAc                                  >  1:906297/1‑150 (MQ=255)
                                                                                                                                             cAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACt          <  1:733035/149‑1 (MQ=255)
                                                                                                                                              aaTGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTcc        >  2:968566/1‑150 (MQ=255)
                                                                                                                                               aTGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTccc       <  2:931385/150‑1 (MQ=255)
                                                                                                                                                tGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCt      <  1:10799/150‑1 (MQ=255)
                                                                                                                                                 ggCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCtt     >  2:917863/1‑150 (MQ=255)
                                                                                                                                                 ggCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCtt     >  2:348045/1‑150 (MQ=255)
                                                                                                                                                    aGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCg   >  1:23150/1‑149 (MQ=255)
                                                                                                                                                    aGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGc  <  2:146555/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACTGGCGGAAGGTATCCGTAAGTTTGCTATTGACCAGGAAAAACTGGAAAAAATGATCGGCGATCTGCTGTAATCATTCTTAGCGTGACCGGGAAGTCGGTCACGCTACCTCTTCTGAAGCCTGTCTGTCACTCCCTTCGC  >  CP009273/8962‑9259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑