| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,005,859 | G→A | 15.6% | G147E (GGG→GAG) | uup → | putative transporter subunit of ABC superfamily: ATP‑binding component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,005,859 | 0 | G | A | 15.6% | 105.4 / 14.1 | 45 | G147E (GGG→GAG) | uup | putative transporter subunit of ABC superfamily: ATP‑binding component |
| Reads supporting (aligned to +/- strand): ref base G (18/20); new base A (2/5); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01 | |||||||||||
AAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAG > CP009273/1005714‑1006008 | aaaCGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGt < 2:729522/148‑1 (MQ=255) aaCGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGt > 2:830508/1‑147 (MQ=255) tATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATcc > 2:263928/1‑149 (MQ=255) tCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGAGTTAGATCCTaa < 1:55056/150‑1 (MQ=255) aCGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGATCTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAATCCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGGTCCTAACg < 2:539063/150‑1 (MQ=255) gATATTTCGCGCCTGGTGATGAGCGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCGGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGtt < 1:724957/150‑1 (MQ=255) atatTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTg < 2:319497/150‑1 (MQ=255) tatTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTgc < 1:23331/150‑1 (MQ=255) tttCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTgcg < 1:548284/149‑1 (MQ=255) cgcCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGAGTTAGATCCTAACGTTGCGCTgtcg > 2:82174/1‑150 (MQ=255) gcCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTgtcgt > 1:1044362/1‑150 (MQ=255) gcCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTgtcgt > 1:131828/1‑150 (MQ=255) ccTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTgtcgtc < 1:805/150‑1 (MQ=255) ccTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTgtcgtc < 1:640527/150‑1 (MQ=255) cTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGtcgtcg > 2:736913/1‑150 (MQ=255) gTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGAGTTAGATCCTAACGTTGCGCTGTCGTCGCt < 2:830831/149‑1 (MQ=255) cgagcgagAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGt < 1:8123/150‑1 (MQ=255) agcgagAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTg < 2:806716/150‑1 (MQ=255) agaAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGt > 1:416023/1‑149 (MQ=255) aaCGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTa < 1:1018802/150‑1 (MQ=255) aCGAACTGGCGAAGGTTCAGGAACAGTTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAg > 1:816302/1‑150 (MQ=255) aCGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAg < 1:363419/150‑1 (MQ=255) tGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGAcgcg > 1:28997/1‑150 (MQ=255) ggCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGAcgcgc > 1:4046/1‑150 (MQ=255) gAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCt > 1:585888/1‑150 (MQ=255) gAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCt > 2:266532/1‑150 (MQ=255) gAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCt > 2:357426/1‑150 (MQ=255) aGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGt > 1:441020/1‑149 (MQ=255) cAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCcg < 1:421348/150‑1 (MQ=255) gATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCcgcgcg < 1:392197/148‑1 (MQ=255) gATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTg < 2:1044362/150‑1 (MQ=255) gATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGAGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCcgcgcg < 1:82174/148‑1 (MQ=255) aTCACCACAACCTTTGTCTGCTGTAAAACCGCATCAACGTTGTTCTTGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCTTCGCTTTCCGGCGGCTGGTTGCTTAAAGCTGCATTTGGACGCGCGCTGGTGAGTAATCCGCGCGTGc > 2:834345/1‑150 (MQ=255) caACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCt > 2:703489/1‑150 (MQ=255) ccTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGAGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTtga < 2:226466/150‑1 (MQ=255) tgGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGaa < 1:736913/150‑1 (MQ=255) gCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAAc < 1:655413/149‑1 (MQ=255) gCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGa > 1:199826/1‑149 (MQ=255) gAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAac < 1:263928/150‑1 (MQ=255) aaCGAAGTGCTGGCGCAACTGGAGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATAtt > 1:466656/1‑150 (MQ=255) cTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCAt < 1:830508/149‑1 (MQ=255) cTGGCGCAACTGGAGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:324663/150‑1 (MQ=255) cAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGActgg < 1:266532/150‑1 (MQ=255) tGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGActggctg > 2:348614/1‑149 (MQ=255) tGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGActggctg > 2:917620/1‑149 (MQ=255) gggTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGa < 1:107967/149‑1 (MQ=255) ggTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAg < 2:199826/150‑1 (MQ=255) | AAACGCTATCACGATATTTCGCGCCTGGTGATGAACGACCCGAGCGAGAAAAATCTCAACGAACTGGCGAAGGTTCAGGAACAGCTGGATCACCACAACCTGTGGCAGCTGGAAAACCGCATCAACGAAGTGCTGGCGCAACTGGGGTTAGATCCTAACGTTGCGCTGTCGTCGCTTTCCGGCGGCTGGTTGCGTAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAG > CP009273/1005714‑1006008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |