| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,977,727 | A→C | 100% | I275R (ATA→AGA) | araG ← | fused L‑arabinose transporter subunits of ABC superfamily: ATP‑binding components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,977,727 | 0 | A | C | 98.0% | 158.6 / ‑4.3 | 49 | I275R (ATA→AGA) | araG | fused L‑arabinose transporter subunits of ABC superfamily: ATP‑binding components |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (29/19); total (29/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATAT > CP009273/1977580‑1977871 | ttACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACCCCCCGCCCCTACCAGACCAAACAACCCAACAATTTCACCACTGCGAACCGCCAGActtctt > 1:197688/1‑150 (MQ=255) tACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGActtctt < 1:1023188/149‑1 (MQ=255) tACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTAtt < 2:823972/149‑1 (MQ=255) cGAATATCGATCGGCTGTTGGTCGATATAACCCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCTCCACTGCGAACCGCCAGACTTCTTGGc < 1:240007/150‑1 (MQ=255) cGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGc < 1:36335/150‑1 (MQ=255) aaTATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCTCTACGCCCCCCCCCCACCAGACCAAACAGCCCAAAAATTTCACCACTGCGAAACGCCAGACCTCTTGGCg > 1:564849/1‑149 (MQ=255) tcgatcgGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTacgc < 2:553339/150‑1 (MQ=255) tGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACAAGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAAAAGCCCAAAAATTTTTCCCCTGCGAACCGCCAGACTTCCTGTCGTACGCACGCCCggg > 2:683242/1‑149 (MQ=255) gTTGGTCGATATAAACCTGACCGGCGGTGATTTTCGTCCCCCCAAACATGCCTTTCATTTATTCGCTACGCCCCCCCCCTTCCCGACCCAACAGCCCCACAATTTTTCCACTTCCAACCCCCCCACTTCTTTGCGTACCCCCCCCTGGtt > 2:867751/1‑149 (MQ=255) ttGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGt < 2:818913/148‑1 (MQ=255) tataAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAg > 2:565832/1‑150 (MQ=255) taAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGc < 1:486632/149‑1 (MQ=255) cTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAAAAATTTTACCACTGCGAACCGCCAGACTTCTTTGCGTACGCACGCCTGGTGCTTTCACAGCATc > 1:142770/1‑147 (MQ=255) cTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAACCCAACAATTTCACCACCGCGAACCGCCCGACTTCTTTGCGTACGCACGCCTGGTGCTTTCACAGCATc > 2:649974/1‑147 (MQ=255) aCCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGa < 2:670835/148‑1 (MQ=255) aCCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGAc < 1:483368/149‑1 (MQ=255) ggcggTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAg > 1:405207/1‑150 (MQ=255) gcggTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAgg > 2:914930/1‑150 (MQ=255) gTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCGACGCCCCGCCCCTACCAGACCAAACAGCCAAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGgcgc < 1:713594/150‑1 (MQ=255) cGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGc > 1:998572/1‑149 (MQ=255) gCCAAACATGCCTTTCATTAATTCGCTGCGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCAAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACt < 1:50428/150‑1 (MQ=255) ccAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTa > 1:79841/1‑150 (MQ=255) cAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACCAc > 1:492755/1‑150 (MQ=255) aaaCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAACCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAcg > 1:225287/1‑150 (MQ=255) aaCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAcgc > 1:850030/1‑150 (MQ=255) aCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAcgcg < 2:695388/150‑1 (MQ=255) gCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTg > 2:139023/1‑150 (MQ=255) aTTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCg > 1:773942/1‑150 (MQ=255) aaTTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCg > 1:183053/1‑147 (MQ=255) gCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATc < 2:998572/150‑1 (MQ=255) cgccccgcccCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGcc > 2:496582/1‑149 (MQ=255) cgccccgcccCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCa < 1:1069429/150‑1 (MQ=255) cccgccccTACCAGACCAAACAGCCCAACAATTGCCCCCCTGCAAACCGCCAGACTTCTTGGGGTACGCACGCCGGGTGCTTTCACACTATCAAGACATAGGCGGTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATg < 2:567001/150‑1 (MQ=255) gccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTcg > 1:139815/1‑150 (MQ=255) cccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTcgcg < 1:841147/150‑1 (MQ=255) aGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGAcc < 1:867751/150‑1 (MQ=255) gACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCa > 1:913310/1‑150 (MQ=255) gACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCa > 2:395419/1‑150 (MQ=255) aCCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCAt > 2:707101/1‑150 (MQ=255) aaaCAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGc > 1:917313/1‑150 (MQ=255) aCAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCt < 1:496496/150‑1 (MQ=255) cccAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCAc > 2:424158/1‑150 (MQ=255) caaTTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAgcg < 2:885580/150‑1 (MQ=255) aaTTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAgcgc > 1:517749/1‑150 (MQ=255) gccacTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTg < 2:564849/149‑1 (MQ=255) cacTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGt > 2:1051602/1‑150 (MQ=255) gCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAAc > 1:626972/1‑150 (MQ=255) gAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACct < 2:142770/150‑1 (MQ=255) ccGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACctgct > 2:328444/1‑150 (MQ=255) cGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACctgctg > 1:29689/1‑150 (MQ=255) gACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCatat > 1:634491/1‑150 (MQ=255) | TTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATAT > CP009273/1977580‑1977871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |