| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,145,170 | G→T | 36.1% | intergenic (+43/+22) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,145,170 | 0 | G | T | 36.1% | 38.5 / 13.5 | 46 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base T (7/10); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
TACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCC > CP009273/2145025‑2145315 | taCGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCt < 2:226758/149‑1 (MQ=255) cgcgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTc < 2:357555/149‑1 (MQ=255) cgcgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTc < 1:460977/149‑1 (MQ=255) cgcgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCa < 2:64725/150‑1 (MQ=255) aaaaTTTACGGCGTTTATACTTTTGTTCTTTTATTGGTCCCCTACCAAAAAAAAGAGCCCGACGAGCAAAGAGAAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTTTTAAAACT‑GGCGTTTGATTTGTTTTCTTCAACCGCCATgag < 2:166270/150‑1 (MQ=255) ggcggTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGGTTAATTTTTTTTTTTAAACCCCCTTAGAAaatcccc > 2:118204/1‑147 (MQ=255) tttATCCCATTGATCTTTTATTGGTCACCTACCAAAAAAAAGAAGCCGACGAGCAAAGAAAAAAATTAGTT‑CGCTAACCAGCCTTTTTATTTATTGTTTAACCG‑GGGGGTTAAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGc < 2:663361/149‑1 (MQ=255) catttGATCTTTTATTGTTCCCCTATCAAAAAAAAGAAGCCGACGAGATAAGAAAAAAATTAGTC‑CGTTAATCAGCCTTTATTTTTTTTTTTAAACCG‑GGGGGTTGATTTTTTGTTTTCAACCGCCATGAGAAACTCACTCTGCCcaca < 2:774961/146‑1 (MQ=255) gATCTTTTATTGGTCCCCTATCCAAAAAAAGACGCCGACGAGCTAAGAGAAAAATTAGTC‑CGCTAATCAGCCGTTATATTTATTGTTTAACCG‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGcc < 1:102628/149‑1 (MQ=255) gATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAgcgc > 1:565754/1‑148 (MQ=255) ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAggg > 1:712955/1‑148 (MQ=255) cccccTATCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTAGTT‑CGCTAATCAGCCTTTATATTTATTTTTAAAACT‑GGCGGTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtttt < 2:245548/147‑1 (MQ=255) acaaaaaGAAAAACCCGACGAGTAAAAAGAAAATTTAGTC‑CGATAAACAGCCTTTATTTTTATTTTTTAACCG‑GGGGGTTGAATTGTTTTCTCCAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatca < 2:663824/147‑1 (MQ=255) ccAAAAGAAGAAGCCGACGAGCTAAGAGAAAAATTATTT‑CGTTAATCAGCCTTTATATTTATTTTTTAAACT‑GGCGGTTGAATTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat < 1:829749/150‑1 (MQ=255) caaaaaGAAGAAGCGGACGAACAAAGAAAAAATTTTGTT‑CGTTAACCAGCTTTTATTTTTATTGTTAAAACG‑GGGGGTTGATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat < 2:489260/148‑1 (MQ=255) aaaaaGAAGAACCCGACGAGCTAAGAGAAAAATTAGTT‑CGCTAATCAGCCTTTATATTTTTTTTTTAAACG‑GGCGTTTAATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat < 1:149539/148‑1 (MQ=255) gaaaaacCCGACGAGATAAAAGAAAATTTAGTC‑CGCTAATCACCATTTATATTTATTGTTTAAACT‑GGCGTTAGAATTGTTGTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc < 1:646792/150‑1 (MQ=255) aaagaagCGGACGAGCAAAGAGAAAAATTGTTT‑CGCAAATCGCCTGTTATTTTTTTTTTTTAACCG‑GGCGGTTAATTTTTTTTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTAc < 1:992287/149‑1 (MQ=255) agaagCCGGAGAGCGAAGA‑TAAAAGTAGTTGCGTTATCCAGCGTTTATTTTTGTTGTTAAACCG‑GGCGGTAGAATTTTTTTCAGAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGc < 2:631923/150‑1 (MQ=255) cgacgaGCTAAGAGAAAAATTATTC‑CGCTAATCAGCCTTTATATTTTTTTTTTAAACT‑GGCGGTTAAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGttt < 1:642574/150‑1 (MQ=255) gacgaGCTAAGAGAAAAATTGTTT‑CTCTAATCACCTTTTTTATTTTTTTTTTAACCG‑GGCGTTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTa < 1:36835/150‑1 (MQ=255) gaGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAATTGTTGTCTTCAACCCCCATGAGAAACTCACTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAccg > 2:882312/1‑150 (MQ=255) gCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTTTTTTCTTCAACCGCCATGAGAAAATTACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTAccgcc > 2:472712/1‑150 (MQ=255) gCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTTTTCCACCGCCCAGAGGAACCCACTTTTCCCCCATCGCCAGTGCCAACGTTTTATCATCCTCCGTACGCTCGTTTAccgcc > 1:899895/1‑150 (MQ=255) cTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTAcccccg > 1:171240/1‑150 (MQ=255) tAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCgg > 1:134061/1‑150 (MQ=255) aaaGAGAAAAATTATTT‑CGATAATCAGCCTTTATATTTTTTTTTAAAACG‑GGGGGTTAATTTTTTGTCTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCgg < 1:215826/149‑1 (MQ=255) aaGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTTTTTTCTTCAACCGCCCTGAGAAACTCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCggg > 2:234226/1‑150 (MQ=255) agagCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTCCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCCCCCGGc > 1:763101/1‑150 (MQ=255) agCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTg > 1:83558/1‑150 (MQ=255) cAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTTTTTTCTTCAACCCCCCTTAGAAACTCACTCTTCCCACAGCGCCCGTGGCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCCGGCTggg > 2:552090/1‑149 (MQ=255) aaaaTTATTT‑CGCTAACCAGCCTTTATTTTTTTTTTTAAAACG‑GGGGGTTGAATTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGAc < 1:131498/150‑1 (MQ=255) aaTTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACa > 2:92458/1‑149 (MQ=255) aTTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTTTTGTCTTCAACCGCCATGAGAAAATCACTCTTCCCACAGCGCCCGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaa > 2:15766/1‑149 (MQ=255) tAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACaaaa > 1:716363/1‑149 (MQ=255) ccaaaaaTCATCCTTTTTTTTTTTTTTTAAACCG‑GGCGGTTAATTTTTTGTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTg < 2:814277/144‑1 (MQ=255) cTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAATTGTTTTCTTTAACCCCCATGAGAAAATCACCCTTCCCACAGCGCCCGTGCCAAAGTTTTTTTATCATCCGTAAGCCCGTTTACCGCCGGGCTGGACAAAAACTGCttt > 2:166773/1‑150 (MQ=255) aGCTGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAAt < 1:535238/150‑1 (MQ=255) tatttattGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCCCCCTGAGAAACTTACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACGCTCGCTTATCGCCGGGCTGGACAAAAAATTCTTTAAAAATTTAGGtaa > 2:217154/1‑150 (MQ=255) tttattGTTTAAACT‑GGCGGTTGAAATGTTGTCTTCAAACGGCATGAGAAAATCACTCTGCCCACAGCGGCAGTGCCAACGTTTTTTCATCAACCGTACGCTCGTTTACCGCCGGGGTGGACAAAAAATGCTTTTAAAATTTAGGtaat > 2:786903/1‑149 (MQ=255) ttttttttAAACCGGGCGGTTAAATTTTTTTTTTAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGtaata < 1:762294/144‑1 (MQ=255) ttttttaaaaCG‑GGGGGTTAATTTGTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATaaa < 2:310271/142‑1 (MQ=255) ttttAAACCGGGCGTTTAATTTGTTGTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATc < 1:983038/148‑1 (MQ=255) aaaCT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAgttg > 2:680311/1‑150 (MQ=255) t‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTcc > 1:880041/1‑150 (MQ=255) ggTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCg > 2:429636/1‑150 (MQ=255) tttgtCTCAAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgcc < 2:565754/148‑1 (MQ=255) tttgcCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTgcc < 1:786903/148‑1 (MQ=255) | TACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTT‑CGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCC > CP009273/2145025‑2145315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |