Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,729,296	A→C	100%	K320Q (AAG→CAG)	xylR →	xylose divergent operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,729,296	0	A	C	100.0%	152.6 / NA	42	K320Q (AAG→CAG)	xylR	xylose divergent operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/21);  total (21/21)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTT  >  CP009273/3729152‑3729441                                                                                                                                                 |                                                                                                                                                  aTTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCCTGAGCAGCGt                                                                                                                                              >  2:448183/1‑150 (MQ=255)       cGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTa                                                                                                                                          >  1:848994/1‑148 (MQ=255)          cgcTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAaag                                                                                                                                       >  1:402477/1‑148 (MQ=255)          cgcACAACAAATTATCGCTCGCCGCCCGACCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAaagaa                                                                                                                                     <  2:367978/150‑1 (MQ=255)                             cgctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAAccatc                                                                                                                   >  1:521852/1‑149 (MQ=255)                                 gACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgc                                                                                                              >  1:1023635/1‑150 (MQ=255)                                  cccgcccccGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgcc                                                                                                             <  2:690855/144‑1 (MQ=255)                                  aCCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatg                                                                                                               <  1:23906/148‑1 (MQ=255)                                    cGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgccat                                                                                                           <  1:125170/150‑1 (MQ=255)                                     gATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgccat                                                                                                           >  1:65815/1‑149 (MQ=255)                                     gATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgccat                                                                                                           >  1:393218/1‑149 (MQ=255)                                             cgTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGc                                                                                                  <  1:275939/150‑1 (MQ=255)                                                  ttCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCgaga                                                                                             <  1:647915/150‑1 (MQ=255)                                                       gCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTg                                                                                        >  2:942218/1‑150 (MQ=255)                                                         cATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTg                                                                                        >  2:1026964/1‑148 (MQ=255)                                                            gCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGgaga                                                                                    >  1:604685/1‑149 (MQ=255)                                                                 aCATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgcg                                                                               <  1:735021/149‑1 (MQ=255)                                                                       gTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTc                                                                        >  2:875900/1‑150 (MQ=255)                                                                        tAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTct                                                                       >  2:472967/1‑150 (MQ=255)                                                                            cACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTctgct                                                                    >  2:522078/1‑149 (MQ=255)                                                                             aCGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTctgctg                                                                   <  2:390246/149‑1 (MQ=255)                                                                             aCGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTctgctg                                                                   <  1:262135/149‑1 (MQ=255)                                                                              cGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAt                                                                 >  1:479065/1‑150 (MQ=255)                                                                               gCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAtt                                                                <  2:521852/150‑1 (MQ=255)                                                                               gCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAtt                                                                >  2:953039/1‑150 (MQ=255)                                                                                ccTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAttt                                                               <  2:355665/150‑1 (MQ=255)                                                                                                    gATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt                                           >  2:537617/1‑150 (MQ=255)                                                                                                         ggTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGat                                      <  2:140094/150‑1 (MQ=255)                                                                                                         ggTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGat                                      <  1:922403/150‑1 (MQ=255)                                                                                                                 aTGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCaaa                              >  1:1058209/1‑150 (MQ=255)                                                                                                                     ggTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAAtgtg                          >  1:1041172/1‑150 (MQ=255)                                                                                                                     ggTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAAtgtg                          <  2:887053/150‑1 (MQ=255)                                                                                                                     ggTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAAtgtg                          >  1:566800/1‑150 (MQ=255)                                                                                                                     ggTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAAtgtg                          >  2:233873/1‑150 (MQ=255)                                                                                                                         gggATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGt                      <  1:233873/150‑1 (MQ=255)                                                                                                                          ggATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGt                      >  1:883776/1‑149 (MQ=255)                                                                                                                                 gcgcTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAGCCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCGCCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATcc                 <  1:359326/147‑1 (MQ=255)                                                                                                                                  cgcTCCAATCCTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCg             <  1:448183/150‑1 (MQ=255)                                                                                                                                    cTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCt           <  1:387125/150‑1 (MQ=255)                                                                                                                                    cTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCt           <  1:667592/150‑1 (MQ=255)                                                                                                                                       cAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCa        <  1:541273/150‑1 (MQ=255)                                                                                                                                         aTCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAat      <  2:393218/150‑1 (MQ=255)                                                                                                                                              gagcAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATAttt  <  2:848994/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  ATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATATTT  >  CP009273/3729152‑3729441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑