BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,097,480	Δ1 bp	100%	coding (1/963 nt)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,097,480	0	A	.	100.0%	708.0 / NA	144	coding (1/963 nt)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (70/74); total (70/74)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 144 total aligned reads displayed.

ATAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGA  >  CP009273/4097335‑4097622

  aaaaTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtc                                                                                                                                         >  2:616318‑M1/1‑148 (MQ=255)

     aTACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcgg                                                                                                                                       >  1:933452‑M1/1‑145 (MQ=255)

      tACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcgga                                                                                                                                      >  2:1089871‑M1/1‑144 (MQ=255)

        ccGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaa                                                                                                                                   <  2:1082951‑M1/150‑9 (MQ=255)

        ccGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaa                                                                                                                                   <  2:234914‑M1/150‑9 (MQ=255)

         cGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaa                                                                                                                                    >  2:1033099‑M1/1‑141 (MQ=255)

          gCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaag                                                                                                                                 >  2:955790‑M1/1‑140 (MQ=255)

          gCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaag                                                                                                                                 >  1:520418‑M1/1‑140 (MQ=255)

            cATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  1:590776‑M1/1‑138 (MQ=255)

             aTTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  1:1079437‑M1/1‑137 (MQ=255)

              tttGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcag                                                                                                                             <  1:2017‑M1/150‑15 (MQ=255)

              tttGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaagg                                                                                                                                >  1:266655‑M1/1‑136 (MQ=255)

                 ggCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagatt                                                                                                                          >  1:175781‑M1/1‑133 (MQ=255)

                 ggCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagat                                                                                                                           <  1:917197‑M1/149‑17 (MQ=255)

                  gCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattc                                                                                                                         <  2:207127‑M1/150‑19 (MQ=255)

                  gCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagatt                                                                                                                          <  1:857376‑M1/149‑18 (MQ=255)

                    ctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcct                                                                                                                       <  1:434287‑M1/150‑21 (MQ=255)

                    ctgacctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcct                                                                                                                       >  1:538427‑M1/1‑130 (MQ=255)

                         ctgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttacc                                                                                                                  >  2:664259‑M1/1‑125 (MQ=255)

                          tgaATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccc                                                                                                                 >  2:897283‑M1/1‑124 (MQ=255)

                            aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                <  1:502352‑M1/149‑28 (MQ=255)

                            aaTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccct                                                                                                                >  1:1025986‑M1/1‑122 (MQ=255)

                             aTCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctga                                                                                                              <  1:332782‑M1/150‑30 (MQ=255)

                              tCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaa                                                                                                             <  2:700213‑M1/150‑31 (MQ=255)

                                aaTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaa                                                                                                            <  2:990845‑M1/149‑32 (MQ=255)

                                aaTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaa                                                                                                            <  2:588548‑M1/149‑32 (MQ=255)

                                 aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                          <  2:734801‑M1/150‑34 (MQ=255)

                                 aTTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaacc                                                                                                          <  2:263445‑M1/150‑34 (MQ=255)

                                   tCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccga                                                                                                        >  1:99339‑M1/1‑115 (MQ=255)

                                    cagcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgat                                                                                                       >  2:41709‑M1/1‑114 (MQ=255)

                                      gcagGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatga                                                                                                     <  1:244639‑M1/150‑39 (MQ=255)

                                            aGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                               >  1:882462‑M1/1‑106 (MQ=255)

                                             gTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                               >  2:220676‑M1/1‑105 (MQ=255)

                                             gTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaag                                                                                               >  2:202925‑M1/1‑105 (MQ=255)

                                              tGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagca                                                                                             <  2:345694‑M1/150‑47 (MQ=255)

                                               gATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagca                                                                                             >  1:297090‑M1/1‑103 (MQ=255)

                                                aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaa                                                                                            <  2:186865‑M1/149‑48 (MQ=255)

                                                aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaa                                                                                            >  2:741804‑M1/1‑102 (MQ=255)

                                                aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaa                                                                                            >  1:352932‑M1/1‑102 (MQ=255)

                                                aTTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaa                                                                                            >  2:871051‑M1/1‑102 (MQ=255)

                                                  tGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaa                                                                                         >  2:17413‑M1/1‑100 (MQ=255)

                                                   gTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaat                                                                                        >  1:688200‑M1/1‑99 (MQ=255)

                                                    ttATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaat                                                                                        <  2:304384‑M1/149‑52 (MQ=255)

                                                    ttATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaat                                                                                        <  2:99339‑M1/149‑52 (MQ=255)

                                                       taCTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcct                                                                                    >  2:566725‑M1/1‑95 (MQ=255)

                                                         cTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctga                                                                                  >  2:798195‑M1/1‑93 (MQ=255)

                                                                cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                           >  2:805694‑M1/1‑86 (MQ=255)

                                                                cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgctt                                                                           >  2:266195‑M1/1‑86 (MQ=255)

                                                                cacaTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgct                                                                            <  1:942710‑M1/149‑64 (MQ=255)

                                                                  caTTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcg                                                                         >  2:650503‑M1/1‑84 (MQ=255)

                                                                          ggATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatca                                                                  <  1:885959‑M1/149‑74 (MQ=255)

                                                                          ggATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattccttta                                                                                                                    <  1:779536‑M1/99‑24 (MQ=255)

                                                                             tCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaagcc                                                              >  2:554541‑M1/1‑73 (MQ=255)

                                                                               aCTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggccta                                                            >  2:283188‑M1/1‑71 (MQ=255)

                                                                                cTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctac                                                           <  1:72925‑M1/150‑81 (MQ=255)

                                                                                cTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctac                                                           <  2:517317‑M1/150‑81 (MQ=255)

                                                                                cTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctac                                                           <  2:601989‑M1/150‑81 (MQ=255)

                                                                                   cGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacat                                                         <  1:566725‑M1/149‑83 (MQ=255)

                                                                                        tgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattc                                                   <  2:30502‑M1/150‑89 (MQ=255)

                                                                                        tgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattc                                                   <  2:1073044‑M1/150‑89 (MQ=255)

                                                                                        tgCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattc                                                   <  1:955790‑M1/150‑89 (MQ=255)

                                                                                         gCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattct                                                  >  1:50877‑M1/1‑61 (MQ=255)

                                                                                           aagaagACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                 >  2:353043‑M1/1‑59 (MQ=255)

                                                                                           aagaagACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                 >  2:667621‑M1/1‑59 (MQ=255)

                                                                                           aagaagACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctg                                                 >  2:956518‑M1/1‑59 (MQ=255)

                                                                                             ggagactTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaa                                              <  2:1064436‑M1/148‑94 (MQ=255)

                                                                                               agaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatt                                            >  2:403022‑M1/1‑55 (MQ=255)

                                                                                                gaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaattt                                           <  1:650503‑M1/150‑97 (MQ=255)

                                                                                                gaCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaattt                                           <  1:629857‑M1/150‑97 (MQ=255)

                                                                                                 aCTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaattta                                          <  1:490029‑M1/150‑98 (MQ=255)

                                                                                                  cTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttat                                         <  1:1018878‑M1/150‑99 (MQ=255)

                                                                                                  cTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttat                                         <  1:998977‑M1/150‑99 (MQ=255)

                                                                                                     ccGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattg                                       <  1:390362‑M1/149‑101 (MQ=255)

                                                                                                      cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaa                                     >  1:193960‑M1/1‑48 (MQ=255)

                                                                                                      cGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaa                                     >  1:4687‑M1/1‑48 (MQ=255)

                                                                                                       ggCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaat                                    <  1:982921‑M1/150‑104 (MQ=255)

                                                                                                        gCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatt                                   <  1:664259‑M1/150‑105 (MQ=255)

                                                                                                              gATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaa                             <  2:802960‑M1/150‑111 (MQ=255)

                                                                                                                tttCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaact                           >  2:1032902‑M1/1‑38 (MQ=255)

                                                                                                                  tCATTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttaccaggcctacatgaattctgcaatttattgaatttgcaaactt                          <  2:294667‑M1/149‑114 (MQ=255)

                                                                                                                    aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactttt                        <  1:17413‑M1/149‑116 (MQ=255)

                                                                                                                    aTTTTGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaactttt                        <  1:249878‑M1/149‑116 (MQ=255)

                                                                                                                        tGCATTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtagg                   >  1:728825‑M1/1‑30 (MQ=255)

                                                                                                                           aTTCCAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccg                <  1:700365‑M1/150‑124 (MQ=255)

                                                                                                                              ccAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccgga              >  2:109397‑M1/1‑24 (MQ=255)

                                                                                                                               cAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggat             >  1:539119‑M1/1‑23 (MQ=255)

                                                                                                                               cAAAGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggat             >  1:361479‑M1/1‑23 (MQ=255)

                                                                                                                                aaaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataa           <  1:616318‑M1/150‑129 (MQ=255)

                                                                                                                                 aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          <  1:667621‑M1/150‑130 (MQ=255)

                                                                                                                                 aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          <  2:538427‑M1/150‑130 (MQ=255)

                                                                                                                                 aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          <  2:527236‑M1/150‑130 (MQ=255)

                                                                                                                                 aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          <  1:41709‑M1/150‑130 (MQ=255)

                                                                                                                                 aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          >  1:61461‑M1/1‑21 (MQ=255)

                                                                                                                                 aaGTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataag          <  1:956518‑M1/150‑130 (MQ=255)

                                                                                                                                   gTTCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggc        >  2:297024‑M1/1‑19 (MQ=255)

                                                                                                                                    ttCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcg       <  1:885240‑M1/150‑133 (MQ=255)

                                                                                                                                    ttCAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtagcccggataaggcg       >  1:643863‑M1/1‑18 (MQ=255)

                                                                                                                                      cAGAGGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgtt     >  1:597729‑M1/1‑16 (MQ=255)

                                                                                                                                       agagGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttc    <  1:353043‑M1/150‑136 (MQ=255)

                                                                                                                                         agGTAGTC‑TGATTtcggaaaaaggcagattcctttaccctgaaaccgatgacagaagcaaaaatgcctgatgcgcttcgcttatcaggcctacatgaattctgcaatttattgaatttgcaaacttttgtaggccggataaggcgttcgc  <  1:798195‑M1/150‑138 (MQ=255)

ATAAAATACCGCCATTTGGCCTGACCTGAATCAATTCAGCAGGAAGTGATTGTTATACTATTTGCACATTCGTTGGATCACTTCGATGTGCAAGAAGACTTCCGGCAACAGATTTCATTTTGCATTCCAAAGTTCAGAGGTAGTCATGATTAAGAAAATCGGTGTGTTGACAAGCGGCGGTGATGCGCCAGGCATGAACGCCGCAATTCGCGGGGTTGTTCGTTCTGCGCTGACAGAAGGTCTGGAAGTAATGGGTATTTATGACGGCTATCTGGGTCTGTATGAAGA  >  CP009273/4097335‑4097622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`