| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | CP009273 | 374,639 | +GCCC | 100% | coding (389/1110 nt) | frmA ← | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | CP009273 | = 374639 | 0 (0.000) | 46 (0.740) +GCCC |
45/258 | NT | 100% | coding (389/1110 nt) | frmA | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase |
| ? | CP009273 | 374640 = | 0 (0.000) | coding (388/1110 nt) | frmA | alcohol dehydrogenase class III/glutathione‑dependent formaldehyde dehydrogenase | |||||
CCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCA > CP009273/374507‑374788 | ccGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaa < 1:13526‑M1/150‑18 (MQ=255)ccGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaaga < 1:929808‑M1/149‑17 (MQ=255) cGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCCGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtg < 1:65120‑M1/150‑28 (MQ=255) gCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtgg > 2:870375‑M1/1‑122 (MQ=255) ccACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtg < 1:1058272‑M1/150‑30 (MQ=255) acaGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgc > 2:573906‑M1/1‑119 (MQ=255) caGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgcc < 1:421109‑M1/150‑33 (MQ=255) gACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggc < 2:291337‑M1/150‑47 (MQ=255) gACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcc > 2:1035975‑M1/1‑104 (MQ=255) aTGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccct > 1:843158‑M1/1‑95 (MQ=255) gTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctggg > 2:97011‑M1/1‑92 (MQ=255) gTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctggg < 1:85183‑M1/150‑59 (MQ=255) gATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaacc < 1:647257‑M1/149‑69 (MQ=255) aaTTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgca < 1:488471‑M1/149‑73 (MQ=255) aTTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaa < 1:639783‑M1/149‑74 (MQ=255) tttGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaaca > 1:307692‑M1/1‑73 (MQ=255) tGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacag < 1:398153‑M1/149‑79 (MQ=255) tGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacag < 1:870375‑M1/149‑79 (MQ=255) ccAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAGGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagag < 1:97011‑M1/149‑81 (MQ=255) cAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggt < 2:678982‑M1/150‑83 (MQ=255) agagACTCTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaagcggaacacagaggtt < 2:674496‑M1/150‑84 (MQ=255) agagACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggt > 1:139326‑M1/1‑67 (MQ=255) gagaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggt > 2:798058‑M1/1‑66 (MQ=255) gaCACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagtt > 2:975567‑M1/1‑64 (MQ=255) acacTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagtt > 2:812891‑M1/1‑63 (MQ=255) cTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttg < 1:873745‑M1/150‑91 (MQ=255) aCTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacg < 1:909678‑M1/150‑99 (MQ=255) cTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacga > 2:802714‑M1/1‑51 (MQ=255) cGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgac < 1:573906‑M1/148‑101 (MQ=255) ttCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcgacacagaggttagttttgccagaacgacagaactca > 1:643050‑M1/1‑41 (MQ=255) aCTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacact > 1:803809‑M1/1‑38 (MQ=255) tGTAGAGCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgca < 2:201552‑M1/150‑119 (MQ=255) agagCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgca > 2:857800‑M1/1‑29 (MQ=255) gagCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcac < 1:857800‑M1/148‑121 (MQ=255) gCACCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccg < 2:560285‑M1/150‑125 (MQ=255) tacCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgc < 2:413824‑M1/149‑126 (MQ=255) aCCCCATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcg > 2:103579‑M1/1‑24 (MQ=255) ccccATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcgg > 2:726775‑M1/1‑23 (MQ=255) ccATGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtg < 2:843158‑M1/150‑130 (MQ=255) ccATGTAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtg > 2:922192‑M1/1‑21 (MQ=255) aTGTAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgt > 1:907951‑M1/1‑19 (MQ=255) tAGTGATAAAGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaag < 2:1024869‑M1/150‑135 (MQ=255) tAGTGATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagc < 2:480290‑M1/150‑135 (MQ=255) gATAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcggga > 1:619163‑M1/1‑12 (MQ=255) aTAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggat < 2:560106‑M1/150‑140 (MQ=255) aTAAAGCGGCTgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcggga < 1:113029‑M1/149‑139 (MQ=255) aaaGCGGCTgcccgcccgttgtaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagaacgacagaactcacactcgccgcactccgcggtgtaaagcgggat > 1:993473‑M1/1‑9 (MQ=255) tgcccgcccgttgaagaaaaacgggtggtgccgtctggcatcaggcctttaccctgggtttcgcgaaccgcaacacagaggttagttttgccagtacgacagaactcacactcgccgcactccgcggtgtaaagcgggatcacatggtcg > 2:867632‑M1/1‑1 (MQ=255) | CCGGTGGTCACGCCACAGCCCAGCAGGCAGACGTGTTCATGGTTTGCTTCTGGATTAATTTTTGCCAGAGACACTTCCGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCA > CP009273/374507‑374788 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |