Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,972 A→C 64.9% A245A (GCA→GCC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,9720AC64.9% 234.8 / 218.6 185A245A (GCA→GCC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base C (65/55);  total (99/86)
Fisher's exact test for biased strand distribution p-value = 8.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

Only 100 of 190 total aligned reads displayed.

GCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACT  >  CP009273/8823‑9121
                                                                                                                                                     |                                                                                                                                                     
  accGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                                                                                                                                     >  2:423907/2‑150 (MQ=255)
  accGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGcccc                                                                                                                                                     >  2:874523/2‑150 (MQ=255)
       cAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCAc                                                                                                                                                <  2:581747/150‑1 (MQ=255)
       cAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgc                                                                                                                                                >  1:399983/1‑148 (MQ=255)
       cAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgc                                                                                                                                                >  1:937410/1‑148 (MQ=255)
       cAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGcgc                                                                                                                                                >  1:788748/1‑148 (MQ=255)
        agaagaTCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGct                                                                                                                                               >  2:1051513/1‑150 (MQ=255)
          aagaTCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGctgc                                                                                                                                             >  2:16312/1‑150 (MQ=255)
              tCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGa                                                                                                                                          >  2:361511/1‑149 (MQ=255)
              tCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGaa                                                                                                                                         <  1:839683/150‑1 (MQ=255)
                cGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGaaa                                                                                                                                        <  2:551430/149‑1 (MQ=255)
                cGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGa                                                                                                                                          >  1:205845/1‑147 (MQ=255)
                cGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAag                                                                                                                                       <  1:657167/150‑1 (MQ=255)
                 gggCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAaga                                                                                                                                      <  1:876856/150‑1 (MQ=255)
                 gggCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGaaa                                                                                                                                        >  2:835926/1‑148 (MQ=255)
                  ggCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAagag                                                                                                                                     >  1:75628/1‑150 (MQ=255)
                  ggCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAagag                                                                                                                                     <  2:980319/150‑1 (MQ=255)
                   gCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGc                                                                                                                                    >  1:652504/1‑150 (MQ=255)
                     gTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTg                                                                                                                                  <  2:228286/150‑1 (MQ=255)
                          ttCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCg                                                                                                                               >  1:207581/1‑148 (MQ=255)
                              gtcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGc                                                                                                                         >  2:660515/7‑150 (MQ=255)
                              gtcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGc                                                                                                                         <  2:617170/144‑1 (MQ=255)
                               tcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGc                                                                                                                         >  2:377873/6‑149 (MQ=255)
                               tcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGc                                                                                                                         >  2:258654/6‑149 (MQ=255)
                               tcagcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCg                                                                                                                        <  1:615614/145‑1 (MQ=255)
                                  gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                                                                                                                     <  1:444002/148‑1 (MQ=255)
                                  gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                                                                                                                     >  2:342550/3‑150 (MQ=255)
                                  gcgAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCATGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAg                                                                                                                     <  1:926537/148‑1 (MQ=255)
                                  cTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAg                                                                                                                     >  2:120129/1‑150 (MQ=255)
                                      aaTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgc                                                                                                                 <  1:420238/150‑1 (MQ=255)
                                      aaTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgc                                                                                                                 >  2:826/1‑150 (MQ=255)
                                      aaTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgc                                                                                                                 <  2:211528/150‑1 (MQ=255)
                                       aTCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgcc                                                                                                                >  1:895445/1‑149 (MQ=255)
                                            cgaGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGgcgccg                                                                                                               <  1:849150/144‑3 (MQ=255)
                                            ccAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTAt                                                                                                              <  1:311892/147‑1 (MQ=255)
                                              aGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAAc                                                                                                         >  1:195967/1‑150 (MQ=255)
                                               gTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACg                                                                                                        >  1:532710/1‑150 (MQ=255)
                                                  ctacAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTaa                                                                                                     <  1:260293/150‑1 (MQ=255)
                                                     caagcagCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTaagc                                                                                                   <  1:1061158/144‑3 (MQ=255)
                                                     caagcagCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCt                                                                                                  >  2:454039/6‑150 (MQ=255)
                                                      aagcagCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTg                                                                                                 <  2:254907/146‑1 (MQ=255)
                                                          agCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCtt                                                                                             <  2:528554/150‑1 (MQ=255)
                                                          agCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTggta                                                                                              >  2:312023/1‑146 (MQ=255)
                                                            cACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTggtatat                                                                                           <  1:1016114/150‑7 (MQ=255)
                                                             aCGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTaca                                                                                          >  2:299565/1‑150 (MQ=255)
                                                             aCGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTaca                                                                                          <  2:168201/150‑1 (MQ=255)
                                                               ggTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACAcc                                                                                        <  2:502284/150‑1 (MQ=255)
                                                                 ttATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCgg                                                                                      >  2:172128/1‑150 (MQ=255)
                                                                  tATGAAACCGTCGTGATGGGCGCAAGCTTCCGCAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAATGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGc                                                                                     >  1:772200/1‑150 (MQ=255)
                                                                   aTGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGc                                                                                     <  2:76763/149‑1 (MQ=255)
                                                                   aTGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGc                                                                                     <  2:1091617/149‑1 (MQ=255)
                                                                      aaaCCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAg                                                                                 <  2:739545/150‑1 (MQ=255)
                                                                           gtcgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGaaa                                                                            <  2:566270/144‑1 (MQ=255)
                                                                           gtcgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGaaa                                                                            <  2:849778/144‑1 (MQ=255)
                                                                           gtcgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGa                                                                              >  1:56540/7‑148 (MQ=255)
                                                                           gtcgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGa                                                                              >  2:888565/7‑148 (MQ=255)
                                                                            tGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAg                                                                           >  1:96783/1‑150 (MQ=255)
                                                                             cgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgc                                                                          >  1:575549/5‑150 (MQ=255)
                                                                             cgtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgc                                                                          >  2:957704/5‑150 (MQ=255)
                                                                              gtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgcg                                                                         >  1:322845/4‑150 (MQ=255)
                                                                              gtgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgcg                                                                         <  2:32484/147‑1 (MQ=255)
                                                                               ttATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAgcgc                                                                        >  2:557449/1‑150 (MQ=255)
                                                                               tgaTGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAgcgc                                                                        <  2:1063389/148‑1 (MQ=255)
                                                                                     gcgcAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCgg                                                                  <  1:1012455/150‑1 (MQ=255)
                                                                                      cgcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgc                                                                 <  1:120129/150‑1 (MQ=255)
                                                                                       gcAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGgcg                                                                <  1:373469/150‑1 (MQ=255)
                                                                                         aaGCTTCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGagcg                                                              >  2:621022/1‑150 (MQ=255)
                                                                                         aaGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                              >  1:1026417/1‑150 (MQ=255)
                                                                                          aGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                              >  2:414501/1‑149 (MQ=255)
                                                                                          aGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGcgcg                                                              >  1:714249/1‑149 (MQ=255)
                                                                                          aGCTTCCG‑AACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTa                                                            <  1:72255/150‑1 (MQ=255)
                                                                                             ttCCGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAt                                                           >  2:92136/1‑149 (MQ=255)
                                                                                               ccGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtta                                                         <  2:879361/149‑3 (MQ=255)
                                                                                               ccGTAACGTTGGCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTAtt                                                          <  1:660515/148‑2 (MQ=255)
                                                                                                    aCATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAg                                                    <  1:831130/149‑1 (MQ=255)
                                                                                                      gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                 <  1:621022/147‑2 (MQ=255)
                                                                                                      gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                 <  2:788748/147‑2 (MQ=255)
                                                                                                      gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                 <  2:1025013/147‑2 (MQ=255)
                                                                                                      gttggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATct                                                 <  1:957704/147‑2 (MQ=255)
                                                                                                         ggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAg                                              >  2:644450/1‑150 (MQ=255)
                                                                                                         ggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAg                                              >  1:435991/1‑150 (MQ=255)
                                                                                                         ggCGAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAg                                              >  2:194038/1‑150 (MQ=255)
                                                                                                         ggCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGa                                               <  1:299565/149‑1 (MQ=255)
                                                                                                         ggCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAg                                              >  2:10253/1‑150 (MQ=255)
                                                                                                            gAAATTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTc                                           >  1:674635/1‑150 (MQ=255)
                                                                                                             aaaTTCTGGAACTGGCTGGCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTcc                                          <  1:16312/150‑1 (MQ=255)
                                                                                                                ttCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCtgt                                       >  2:938185/1‑150 (MQ=255)
                                                                                                                 tCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCtgtg                                      >  2:588044/1‑150 (MQ=255)
                                                                                                                        ctggctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagca                                >  1:1079088/1‑149 (MQ=255)
                                                                                                                           gctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagcata                              <  1:983101/145‑3 (MQ=255)
                                                                                                                           gctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGgcagcata                              <  1:590746/145‑3 (MQ=255)
                                                                                                                            ctggCTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAAc                            >  1:124475/3‑149 (MQ=255)
                                                                                                                             aGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAAc                            >  1:809919/1‑148 (MQ=255)
                                                                                                                                cTGTGACCGCCTGACCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGa                       >  2:753028/1‑150 (MQ=255)
                                                                                                                                  gCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATc                     >  1:602020/1‑150 (MQ=255)
                                                                                                                                    gACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATcc                    >  2:774188/1‑149 (MQ=255)
                                                                                                                                            gACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAg            >  1:561107/1‑149 (MQ=255)
                                                                                                                                             aCCATCGCCCCGGCGCTGCTGAAAGAGCTGTCCGAAAGCGAAGGCGCCGTTGAGCGTAAGCTGGTATATACCGGTGAAGTGAAAGCGCGTCCGGAGCGTATTACCGAATCTGAGTTCCTGTGGCAGCATAACCAGGATCCGATGGCGGTa          <  2:28097/150‑1 (MQ=255)
                                                                                                                                             aCCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAg            <  1:172128/148‑1 (MQ=255)
                                                                                                                                                     aCCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACt  >  1:449882/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCTGCGACCGTCTGACCATCGCACCGGCACTGCTGAAAGAGCTGGCGGAGAGCGAAGGGGCTATCGAACGTAAACTGTCTTACACCGGCGAAGTGAAAGCGCGTCCGGCGCGTATCACTGAGTCCGAGTTCCTGTGGCAGCACAACCAGGATCCAATGGCAGTAGATAAACT  >  CP009273/8823‑9121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑