Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,230,349 T→C 39.7% intergenic (‑176/‑45) nhaB ← / → fadR sodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,230,3490TC39.7% 37.2 / 71.1 58intergenic (‑176/‑45)nhaB/fadRsodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (21/14);  new base C (13/10);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

AATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGA  >  CP009273/1230202‑1230494
                                                                                                                                                   |                                                                                                                                                 
aaTGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGAAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTat                                                                                                                                                 >  2:68609/1‑150 (MQ=255)
 atgatgTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTat                                                                                                                                                 >  1:244818/1‑149 (MQ=255)
         tttATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTc                                                                                                                                         >  1:215863/1‑149 (MQ=255)
           tATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCa                                                                                                                                       <  1:100732/149‑1 (MQ=255)
           tATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCaa                                                                                                                                      >  1:814954/1‑150 (MQ=255)
           tATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCa                                                                                                                                       <  1:453421/149‑1 (MQ=255)
            aTGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAAc                                                                                                                                     >  1:144370/1‑150 (MQ=255)
             tGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACt                                                                                                                                    >  1:936554/1‑150 (MQ=255)
              gTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACtt                                                                                                                                   >  2:496935/1‑150 (MQ=255)
                 tAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGt                                                                                                                                >  2:624883/1‑150 (MQ=255)
                     cGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTg                                                                                                                            >  2:481016/1‑150 (MQ=255)
                       cAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCt                                                                                                                          >  1:862374/1‑150 (MQ=255)
                       cAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCt                                                                                                                          >  1:325728/1‑150 (MQ=255)
                          aGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCtgtg                                                                                                                       <  2:1096801/150‑1 (MQ=255)
                          aGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCtgtg                                                                                                                       <  1:402788/150‑1 (MQ=255)
                                 cGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATgg                                                                                                                 >  2:339667/1‑149 (MQ=255)
                                           ggAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTa                                                                                                      <  2:729956/150‑1 (MQ=255)
                                             aGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATg                                                                                                    >  1:951250/1‑150 (MQ=255)
                                                                    aGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCcggc                                                                             >  1:249199/1‑150 (MQ=255)
                                                                    aGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCcggc                                                                             >  2:842214/1‑150 (MQ=255)
                                                                     ggggAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCcggcg                                                                            <  2:133472/150‑1 (MQ=255)
                                                                      gggAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCcggcgg                                                                           >  2:455622/1‑150 (MQ=255)
                                                                        gAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGt                                                                         <  2:144370/150‑1 (MQ=255)
                                                                             agaTGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTcgc                                                                    <  1:61992/150‑1 (MQ=255)
                                                                             agaTGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTcgc                                                                    <  1:496935/150‑1 (MQ=255)
                                                                                tGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCgg                                                                  >  1:570597/1‑149 (MQ=255)
                                                                                tGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCgg                                                                  >  2:174845/1‑149 (MQ=255)
                                                                                tGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCgg                                                                  >  2:116054/1‑149 (MQ=255)
                                                                                   ggAAAATGAAGCCTTGATACCTTTATCTTATTTTTGTCTGCTATCAGCGTAGTTAGCCATCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCAATATGGTCATTAAGGCGCAAAGCCAGGCGGGTTACGCGGAaga                                                              <  2:862374/150‑1 (MQ=255)
                                                                                    gAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAagag                                                             <  2:814954/150‑1 (MQ=255)
                                                                                      aaaTGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTa                                                           >  2:111875/1‑150 (MQ=255)
                                                                                       aaTGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTa                                                           >  2:870880/1‑149 (MQ=255)
                                                                                        aTGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACa                                                         >  1:350634/1‑150 (MQ=255)
                                                                                        aTGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACa                                                         >  1:480497/1‑150 (MQ=255)
                                                                                         tGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACat                                                        <  1:141031/150‑1 (MQ=255)
                                                                                           aaGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACatta                                                      <  1:339667/150‑1 (MQ=255)
                                                                                           aaGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACatta                                                      <  2:1027185/150‑1 (MQ=255)
                                                                                              ccTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACattatt                                                    >  1:621645/1‑149 (MQ=255)
                                                                                               cTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTg                                                   >  1:487846/1‑149 (MQ=255)
                                                                                                 tGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGa                                                  >  2:765097/1‑148 (MQ=255)
                                                                                                   aTCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAg                                               <  1:481016/149‑1 (MQ=255)
                                                                                                      ccTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTAt                                            <  2:941779/149‑1 (MQ=255)
                                                                                                          tttCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTg                                         <  2:215863/148‑1 (MQ=255)
                                                                                                           ttcttcTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGa                                       <  1:68609/149‑1 (MQ=255)
                                                                                                             cttcttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAAt                                     <  2:951250/149‑1 (MQ=255)
                                                                                                               tcttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAAc                                  >  1:929119/1‑150 (MQ=255)
                                                                                                               tcttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAAc                                  >  2:1066258/1‑150 (MQ=255)
                                                                                                               tcttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAAc                                  >  1:744577/1‑150 (MQ=255)
                                                                                                                cttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACCTTATTGAAAGTATCTGGAATAAcc                                 >  1:16557/1‑150 (MQ=255)
                                                                                                                   tttGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCGCTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAAcc                                 <  2:249199/147‑1 (MQ=255)
                                                                                                                      gTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtc                            <  2:333360/149‑1 (MQ=255)
                                                                                                                       tCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtccc                          >  2:962053/1‑150 (MQ=255)
                                                                                                                        cTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtccct                         >  2:568960/1‑150 (MQ=255)
                                                                                                                           cTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtccctccc                      <  2:325728/150‑1 (MQ=255)
                                                                                                                            tATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCg                     >  1:128915/1‑150 (MQ=255)
                                                                                                                                   gTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTAt              >  1:888688/1‑150 (MQ=255)
                                                                                                                                      gTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTAtttt           <  2:744577/150‑1 (MQ=255)
                                                                                                                                                  gTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGa  <  2:487846/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGA  >  CP009273/1230202‑1230494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑