Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,555,008 T→C 8.2% D60G (GAC→GGC)  ddpB ← D‑ala‑D‑ala transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,555,0080TC 8.2% 537.9 / 4.7 210D60G (GAC→GGC) ddpBD‑ala‑D‑ala transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (108/75);  new base C (2/19);  total (115/95)
Fisher's exact test for biased strand distribution p-value = 1.17e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCA  >  CP009273/1554859‑1555156
                                                                                                                                                     |                                                                                                                                                    
  agcagGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTc                                                                                                                                                     >  1:10688/1‑149 (MQ=255)
      gggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTc                                                                                                                                                <  1:219969/150‑1 (MQ=255)
       ggCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTcc                                                                                                                                               <  1:1062458/150‑1 (MQ=255)
          aCCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGCAAAACTGTACATACAGTGGCTGGTCCAGTCCCAg                                                                                                                                            >  1:366008/1‑150 (MQ=255)
            cAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGtt                                                                                                                                          >  1:526692/1‑150 (MQ=255)
             aaaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTg                                                                                                                                         >  2:181363/1‑150 (MQ=255)
              aaaGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGc                                                                                                                                        >  1:473310/1‑150 (MQ=255)
                aGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                                                                                                      >  1:838143/1‑150 (MQ=255)
                aGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTg                                                                                                                                      <  1:260827/150‑1 (MQ=255)
                 gCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTgg                                                                                                                                     >  2:1047307/1‑150 (MQ=255)
                   cAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGc                                                                                                                                    >  1:829879/1‑149 (MQ=255)
                   cAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCg                                                                                                                                   <  2:388222/150‑1 (MQ=255)
                    aGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGCCCAGTCCCAGTTGCTgggg                                                                                                                                   >  2:789009/1‑147 (MQ=255)
                     gCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGaa                                                                                                                                 >  1:173221/1‑150 (MQ=255)
                        ccAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAatat                                                                                                                              >  1:142429/1‑150 (MQ=255)
                        ccAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAatat                                                                                                                              >  2:803722/1‑150 (MQ=255)
                         cAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATAtt                                                                                                                             >  1:771669/1‑150 (MQ=255)
                            cGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATAttttt                                                                                                                          >  1:1061133/1‑149 (MQ=255)
                             gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCTGGCGCATATTTTc                                                                                                                          >  2:880156/1‑149 (MQ=255)
                             gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATAttttt                                                                                                                          >  2:18338/1‑148 (MQ=255)
                             gTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTc                                                                                                                          >  2:944600/1‑149 (MQ=255)
                                 ccGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGa                                                                                                                     >  2:560333/1‑150 (MQ=255)
                                  cGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAt                                                                                                                    <  1:631594/150‑1 (MQ=255)
                                  cGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAt                                                                                                                    <  1:489819/150‑1 (MQ=255)
                                  cGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGAt                                                                                                                    <  2:543209/150‑1 (MQ=255)
                                   gggAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATa                                                                                                                   <  2:521365/150‑1 (MQ=255)
                                     gAAAAATATACGCATCTCTACCAGCACCGGACGCCCGGTACGAATGAATGTTCCCAGGTCACCATGAAAGAGGTCGCTGACGTAGCGGTAAAACTGTACATCCAGTGGCAGGTCCAGTCCCAGTTGCTAGCCCAGAATTTCCACGAAAGc                                                                                                                 <  2:586674/150‑1 (MQ=255)
                                     gAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGc                                                                                                                 <  1:979021/150‑1 (MQ=255)
                                      aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCa                                                                                                                <  1:250867/150‑1 (MQ=255)
                                      aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCa                                                                                                                >  2:866036/1‑150 (MQ=255)
                                      aaaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCa                                                                                                                >  1:857402/1‑150 (MQ=255)
                                       aaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                                                                                               <  1:565009/150‑1 (MQ=255)
                                       aaaaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAt                                                                                                               >  1:729468/1‑150 (MQ=255)
                                         aaTATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCg                                                                                                             >  1:773697/1‑150 (MQ=255)
                                               cGCAGCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAGCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTGCCACGATAGCATCGCTGgc                                                                                                        <  2:929203/149‑1 (MQ=255)
                                                 cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                                                                                                     <  2:36817/150‑1 (MQ=255)
                                                 cAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGcgcg                                                                                                     <  2:783297/150‑1 (MQ=255)
                                                   aCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGt                                                                                                   >  2:845278/1‑150 (MQ=255)
                                                   aCTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGt                                                                                                   <  2:408657/150‑1 (MQ=255)
                                                         ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAc                                                                                                <  1:455242/147‑1 (MQ=255)
                                                         ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCg                                                                                             >  1:689606/1‑150 (MQ=255)
                                                         ccAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCg                                                                                             <  2:1061133/150‑1 (MQ=255)
                                                              aCCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg                                                                                         >  1:478853/1‑149 (MQ=255)
                                                               ccGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGt                                                                                        >  1:652379/1‑149 (MQ=255)
                                                               ccGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGt                                                                                        >  1:410144/1‑149 (MQ=255)
                                                               ccGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCCGGCGCGGGCACCCGCCAGt                                                                                        >  2:889002/1‑149 (MQ=255)
                                                                cGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTaa                                                                                      >  1:66567/1‑150 (MQ=255)
                                                                 ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc                                                                                     >  2:1065565/1‑150 (MQ=255)
                                                                   aCGCCCGGTACGAATGGATGTTCCCAGGTCACAATGAAACAGGTCGCTGACGTAGGGGAAAAACTGTACATACAGTGGCTGGCCCGGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGGGGTCCCCCGCCAGTAACcg                                                                                   <  2:260635/150‑1 (MQ=255)
                                                                     gCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGGCACCCGCCAGTAACcgcg                                                                                 >  2:324112/1‑150 (MQ=255)
                                                                         ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGc                                                                                                                 >  1:174800/1‑114 (MQ=255)
                                                                          gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa                                                                            >  1:582583/1‑150 (MQ=255)
                                                                          gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGATGCTGGCGAATATTTTCCACGATAGCATCGCCGGCGCGGTCACCCGCCCGGAACCGGGCCGGa                                                                            >  1:710041/1‑150 (MQ=255)
                                                                             cGATTGGATGTCCCCGGGTCACCATGAAACAGGTCGCTGAGGTGGGGGAAAAACTGTACATACGGTGGCTGGTCCAGTCCCGGTTGCTGGAGAATTTTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCa                                                                         <  1:880156/150‑1 (MQ=255)
                                                                              gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCTGGCGAATATTTTCCACGATAGCATCGCTTGCGCGGTCACCCGCCCGTAACCGCGCCCGATCAc                                                                        >  2:756362/1‑150 (MQ=255)
                                                                              gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACCATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                        >  1:818456/1‑150 (MQ=255)
                                                                               aaTGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGCCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCCTCGCTTGCCCGGACACCCGCCAGTAACCGCGCCGGAGCAc                                                                        >  1:1053111/1‑149 (MQ=255)
                                                                                  ggATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAgg                                                                    >  1:405816/1‑150 (MQ=255)
                                                                                   gATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                   >  2:901180/1‑150 (MQ=255)
                                                                                    aTGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCCCTAACCGCGCCGGGTCCCCAGGGa                                                                  >  2:81451/1‑150 (MQ=255)
                                                                                     tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                                                 >  1:284079/1‑150 (MQ=255)
                                                                                       ttCCCAGCTCCCCATAAAAGGGGTCGCGGACGTGGGGGAAAACCTGTACATAGAGTGGCGGGTCGAGCCCCAGTTGCGGGCAAATTTTTCCCAAGAAGGAAGCGCGGGCGCGGGCACCCGCCAGTAACCGCGCCGGATCACCAGGGATc                                                                <  2:438031/149‑1 (MQ=255)
                                                                                       tccccAGGCCACCATGAAACAGGGCGCTGACGAAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGCCCCGGTTGCTGGCAAATATTTTCCACGATAGCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCa                                                               <  1:182716/148‑1 (MQ=255)
                                                                                         cccAGGCCCCAATGAAACAGGTCGCTGACGTGGGGGAAAAACTGTACATACAGTGGCTGGTCCAGCCCCAGTTGCGGGCGAATATTTCCCAGGATAGCATCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAg                                                              <  1:201328/149‑1 (MQ=255)
                                                                                           cAGGTCCCCATAAAACAGGTCGCTGACGTAGCGGTAAACCTTTACATAAGGGGGCTGGCCCAGTCCCAGTTGCGGGCGAATTTTTCCCACGATAGCACCGCTGGCGGGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                           <  2:661111/150‑1 (MQ=255)
                                                                                           cAGGTCACCATAAAACAGGTCGCGGACGTAGGGGTAAAACTGTACATACGGTGGCTGGCCCAGTCCCAGTTGCGGGCAAATTTTTCCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                           <  1:1045840/150‑1 (MQ=255)
                                                                                            aGGTCACAATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCGGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGc                                                          <  1:912000/150‑1 (MQ=255)
                                                                                               tCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGGTCACCAGGGATCAGATGc                                                          >  1:299254/1‑147 (MQ=255)
                                                                                                cACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACGGTGGCTGGTCCAGCCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgaga                                                      <  1:43718/150‑1 (MQ=255)
                                                                                                   cATGAAACAGGTCGCGGACGTAGCGGTAAAACTGTCCATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                   <  1:1001760/150‑1 (MQ=255)
                                                                                                    aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                   >  1:23361/1‑149 (MQ=255)
                                                                                                     tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                                                  >  1:948801/1‑149 (MQ=255)
                                                                                                       aaaCAGGATGCTGACGTATCGGTAAAACTGTACATACAGTTGCTGGTCCTCTTCCAGTTTCCGGCGTACATTTTCCCCGAATGCATGCCGGGCGCGGACACGCCCCAGTCACCGCGCCGGAACCCCACTGATCAGACGCGAGATAATaaa                                               >  2:179480/1‑150 (MQ=255)
                                                                                                          cAGGGCGCGGACGTGGGGGTAAAACTGTACATACAGGGGCTGGTCCGGCCCCTGTTGCGGGCAAATTTTTCCCACGATGGCATCGCTGGCGCGGTCCGCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGt                                            <  1:789009/150‑1 (MQ=255)
                                                                                                            ggTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGGCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTa                                           >  2:592728/1‑149 (MQ=255)
                                                                                                              tCGCTGCCGTAGCGGTAAATCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCGGGCGAATTTTTTCCACGATACCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                        <  1:803722/150‑1 (MQ=255)
                                                                                                              gcGCTGACGTGGGGGAAAAACGGAACATGCGGGGGGTGGCCCAGTCCCGGTTGCGGGCAAATATTTCCCAGGAAAGCATCGCGGGGGCGGCCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                        <  1:502355/149‑1 (MQ=255)
                                                                                                               cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCa                                       >  2:152343/1‑150 (MQ=255)
                                                                                                                gCTGACGTAGGGGAAAAACTGTACAAAAAGGGGCTGGCCCAGCCCCAGTGGCGGGCGAATATTTACAACGATAGCATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCAc                                      <  1:1091312/150‑1 (MQ=255)
                                                                                                                gCTGACGTAGCGGAAAACCTGTACATACAGTGGCTGGTCCAGCCCCAGTTGCTGGCAAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCa                                       <  1:32490/149‑1 (MQ=255)
                                                                                                                    aggtgggggTAAATCTGTACATACAGTGGCTGGCCCAGCCCCGGTGGCGGGCAAATATTTCCCACGATAGCATCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGc                                    <  2:201098/141‑1 (MQ=255)
                                                                                                                      gTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAc                                >  2:682285/1‑150 (MQ=255)
                                                                                                                       gggCGGAAAAACTGTCCATACAGGGGCTGGCCCAGCCCCGGTTGCGGGAGAATATTTCCCACGATAGCAGCGCGGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcg                               <  2:735391/148‑1 (MQ=255)
                                                                                                                           ggTAAAACTTTACATCAGGTGGCTGGCCCAGCCCCAGTTGCTGGCGAATATTTCCCACGATAGCATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccg                           <  2:403016/150‑1 (MQ=255)
                                                                                                                           ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCCGTTGCTGGCGAATATTTTCCACGATAGCATCCCTGGCGCGGTTACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGGTAATAAACGTAATCACGCAGAcgcccg                           >  2:429778/1‑150 (MQ=255)
                                                                                                                           ggTAAAACTGTACATACAGGGGCTGGTCCAGCCCCAGTGGCGGGAAAATATTTCCAACGAGAGCATCGCGGGGGCGGCCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccg                           <  1:516631/150‑1 (MQ=255)
                                                                                                                                     tacatacgGGGGTGGGCCCACTCCAGGTTGCTGGAAAATTTTTCCCAGAATGCCAGCGCTGGCGGGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACg                   <  2:473310/148‑1 (MQ=255)
                                                                                                                                      acatacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAg                 >  2:972565/1‑149 (MQ=255)
                                                                                                                                       catacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCa               >  1:932129/1‑150 (MQ=255)
                                                                                                                                         tacgggggCTGGTCCGGCCCATGTTGCGGGCAAATTTTTCCCACAAACGCACCGCTGGGGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAAAGTAATCACGCAGACGCCCGCCACCACGAGCAcc             <  2:729468/144‑1 (MQ=255)
                                                                                                                                         tacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCTCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGGTCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCAc              >  1:738500/1‑149 (MQ=255)
                                                                                                                                         tacaGTGGCTGGCCCAGCCCCAGTTGCGGGCGAATATTTCCCACGATAGCATCGCGGGCGCGGTCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCAcc             <  1:308223/150‑1 (MQ=255)
                                                                                                                                             gTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcc         >  1:1021441/1‑150 (MQ=255)
                                                                                                                                                gttGGTCCAGCCCCAGTGGCGGGCAAATATTTCCCACGATAGCACCGCGGGCGCGGCCCCCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGccccc      <  1:889002/148‑1 (MQ=255)
                                                                                                                                                gCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcccc       >  2:616472/1‑149 (MQ=255)
                                                                                                                                                gCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcccc       >  1:749569/1‑149 (MQ=255)
                                                                                                                                                 cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcccc       >  1:222667/1‑148 (MQ=255)
                                                                                                                                                  tGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcag    >  1:153091/1‑150 (MQ=255)
                                                                                                                                                   ggTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcag    >  2:278465/1‑149 (MQ=255)
                                                                                                                                                   ggTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCcag    >  1:847294/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCAGCAGGGCACCAAAAGCCAGCTCCAGCGTCGCCGGGAAAAATATACGCAACTCTTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCA  >  CP009273/1554859‑1555156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑