| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,837,194 | T→A | 22.9% | N189K (AAT→AAA) | gdhA → | glutamate dehydrogenase, NADP‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,837,194 | 0 | T | A | 22.9% | 67.0 / 2.0 | 42 | N189K (AAT→AAA) | gdhA | glutamate dehydrogenase, NADP‑specific |
| Reads supporting (aligned to +/- strand): ref base T (5/26); new base A (8/2); total (14/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGCGTTTCCGTTTCTGG > CP009273/1837048‑1837343 | ggTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATAcc < 2:886746/150‑1 (MQ=255) gAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAATTTCCAACAAAACCGcc > 2:1060141/1‑150 (MQ=255) tGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCt < 2:380176/150‑1 (MQ=255) ttttGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCAc < 1:393053/149‑1 (MQ=255) aGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAgg < 1:833459/150‑1 (MQ=255) gcgcTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTTGAAAAAAGCTCCCACAATACCCCCCCCGTCTTCCCC‑GGTAAgggg > 2:574120/1‑149 (MQ=255) gcTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCt < 2:669223/150‑1 (MQ=255) gaCTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAAATCTCCAACAAAAACCCCCGCGGCTTTCCC‑GGGAAGGGGctttcttt > 1:850336/1‑150 (MQ=255) cTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGTAAAAACGCTCCCACAAAACCGCCCGCGTCTTCCCC‑GGTAAGGGGCTTTTATTTg > 2:480822/1‑150 (MQ=255) aTCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGATGAAAAAAATTTCCCACAACAACCCCCCCGTGTTCCCC‑CGTTAGGGGCTCTTATTTtgcggccggc > 2:69804/1‑146 (MQ=255) tCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTc < 1:480822/149‑1 (MQ=255) gCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCtt < 1:56782/149‑1 (MQ=255) cACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGATGAAAAAAATCCCCAACAAAAACCCCCCCGTCCTCACC‑GGGAAGGGCCCTTCATTTTGCGGGAGTCTTAtt > 1:134837/1‑150 (MQ=255) tGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTTATGAAAAAAATCTCCAACAAAAACGCCCGCGTCTTCACC‑GGGAAGGGGCTTTTTTTTTGCGGCCGTCTTTTTTGcc > 1:36408/1‑150 (MQ=255) ggCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGGGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGccc < 1:492238/149‑1 (MQ=255) gATACCGACGTTCCGGCGGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTAGGGCGGGGATGATGAAAAAGCTCTCCAAGAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCt < 2:983157/150‑1 (MQ=255) ccGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTAcc < 1:574120/149‑1 (MQ=255) ccGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTAcc < 2:416933/149‑1 (MQ=255) gACGTTCCGGCGGGTGATGTCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGc < 1:877445/150‑1 (MQ=255) ccGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGGGGTGAAAAAACTCTCCCACAAAAACACCCGCGCCTTCTCCGGGAAAGGGCTTTTCTTTTGG‑GGCCGGCTTATTTTCCCCGGAGCTACCCGCTACggg > 1:82553/1‑149 (MQ=255) cGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGGTGGTTAAAAAACTCTCCCACAAAAACGCCTGCGTCCTTACC‑CGTAAGGGGCTTTTTTTTTTCGGCAGTCTTTTTTGCCCCGGAGCTTCCCGCCCCGGTc > 1:643772/1‑150 (MQ=255) gTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGttt < 2:297906/150‑1 (MQ=255) tGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTa < 2:869286/150‑1 (MQ=255) atatCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTAtt < 2:344247/150‑1 (MQ=255) atatCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTAATCGCCCGGAAGCTACCGGCTACGGTCTGGTTTAtt < 2:463671/150‑1 (MQ=255) ggggTTGGTGATCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTcaca < 2:978075/150‑1 (MQ=255) ttGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAg < 2:1099558/150‑1 (MQ=255) tggtCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAAt < 1:530049/150‑1 (MQ=255) tggtCATAAAGTCGGCTTTATGGCGGAGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAAt < 2:58756/150‑1 (MQ=255) gTGAAGTCGGCTTTATGGCGGGGATTATGAAAAAGTTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTa < 2:36408/149‑1 (MQ=255) tGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTaaa < 2:257248/150‑1 (MQ=255) ggCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCAc < 2:134837/150‑1 (MQ=255) aTGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTAt < 2:187894/149‑1 (MQ=255) aTGGCGGGGATGATGAAAAAACTCTCCAACAAAACCCCCTGCGTCTTCACC‑GGGAAGGGGCTTTCATTTTGCGGGAGTCTTATTTGCCCGGAAGCTACCGGCTACGGTCTGGTTTTTTTTACAAAAACAATGCTAAAAAGCCACGGGATg > 2:454775/1‑150 (MQ=255) ggggATGATGAAAAAGCTCTCCAACAATACCGCCGGCGTCTTCACC‑GGTAAGGTTTTTGCATTTGGCGGCAGTCTTATTCTCCCGTGAGCTACCGGCTACGGGCTGGTTTATTTCACAGAAGCGATGCTAAAACGCTACGTTTTGGGttt > 2:669076/1‑150 (MQ=255) ggATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGtttt > 2:780058/1‑149 (MQ=255) aaaaaGCCCTCAAACACAGCCGCCTGAGTATTCCCC‑GGTAAGGGCCTTTCATTTGGCGGCCGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGAt < 1:669076/149‑1 (MQ=255) ctctCCAAAAAAGCCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGGAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGcgtt < 2:386647/150‑1 (MQ=255) caacaaTACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGcgtttccgt > 1:738009/1‑150 (MQ=255) caacaaTACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGcgtttccgt < 1:662414/150‑1 (MQ=255) aacaaTACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGcgtttccgtt > 2:524397/1‑150 (MQ=255) acaaTACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGcgtttccgttt < 1:462625/150‑1 (MQ=255) tACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGCGTTTCCGTTTCTgg < 1:69804/150‑1 (MQ=255) tACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGCGTTTCCGTTTCTgg > 1:1070022/1‑150 (MQ=255) | GGTGAAGTGATGCGTTTTTGCCAGGCGCTGATGACTGAACTGTATCGCCACCTGGGCGCGGATACCGACGTTCCGGCAGGTGATATCGGGGTTGGTGGTCGTGAAGTCGGCTTTATGGCGGGGATGATGAAAAAGCTCTCCAACAATACCGCCTGCGTCTTCACC‑GGTAAGGGCCTTTCATTTGGCGGCAGTCTTATTCGCCCGGAAGCTACCGGCTACGGTCTGGTTTATTTCACAGAAGCAATGCTAAAACGCCACGGTATGGGTTTTGAAGGGATGCGCGTTTCCGTTTCTGG > CP009273/1837048‑1837343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |