| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,074,590 | C→G | 22.5% | A135A (GCG→GCC) ‡ | tktA ← | transketolase 1, thiamine triphosphate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,074,590 | 0 | C | G | 22.5% | 668.3 / 205.6 | 284 | A135A (GCG→GCC) ‡ | tktA | transketolase 1, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base C (113/107); new base G (42/22); total (155/129) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
Only 100 of 306 total aligned reads displayed.
TTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC > CP009273/3074441‑3074739 | ttGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTgc < 2:155112/150‑1 (MQ=255) tCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgcc < 1:743021/150‑1 (MQ=255) cATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGcgccg > 2:599530/1‑150 (MQ=255) gtCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGgcccgc > 2:690201/2‑150 (MQ=255) tCGTAGACGGCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGcccgcc < 1:457081/150‑1 (MQ=255) tCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCgccgcc < 1:979088/150‑1 (MQ=255) gTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAg > 2:431619/1‑150 (MQ=255) tagacggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGc > 1:592129/7‑150 (MQ=255) tAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGc > 2:323065/1‑150 (MQ=255) aGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCg > 1:957520/1‑150 (MQ=255) gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGt < 2:496285/150‑1 (MQ=255) gAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGt > 2:507948/1‑150 (MQ=255) acggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcg > 1:833317/4‑148 (MQ=255) aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtt < 1:70121/150‑1 (MQ=255) aaTGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtt < 1:636850/150‑1 (MQ=255) cggCAATCAGTTTGCCCAGTTTCATCGAGCCTGCCAATGAGCAAGCTTCATGCGAGATGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCgcgc < 1:773462/148‑4 (MQ=255) tGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttt > 2:65278/1‑150 (MQ=255) aTCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCt < 1:479053/150‑1 (MQ=255) cAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCa > 1:1043467/1‑137 (MQ=255) gTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGtttttt > 1:64232/1‑144 (MQ=255) tACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATc < 2:790850/149‑1 (MQ=255) tACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCg > 2:287734/1‑150 (MQ=255) aCCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCg < 1:134269/149‑1 (MQ=255) ccAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCa > 2:976632/1‑150 (MQ=255) aGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATa < 2:377867/150‑1 (MQ=255) gCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATAc > 1:626733/1‑150 (MQ=255) cTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATAcc > 2:1099729/1‑150 (MQ=255) aGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACt < 2:756688/150‑1 (MQ=255) gCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTg > 2:142976/1‑150 (MQ=255) tACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGt > 1:42698/1‑150 (MQ=255) cAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCaa > 1:453553/1‑149 (MQ=255) agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAcc < 1:1001966/150‑1 (MQ=255) agagCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATAcc < 1:247952/150‑1 (MQ=255) agCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCt < 1:352162/150‑1 (MQ=255) aaCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc < 1:865245/150‑1 (MQ=255) aCTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGAcc > 2:1089538/1‑149 (MQ=255) cTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCa < 2:610578/150‑1 (MQ=255) cGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAg < 2:299857/148‑1 (MQ=255) gagaTGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCg < 2:235971/150‑1 (MQ=255) gagaTGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCg > 1:509927/1‑150 (MQ=255) agaTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCgg > 1:572821/1‑150 (MQ=255) aTGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTTACCTAACGGTCCggtc > 1:557296/1‑149 (MQ=255) aTGCCTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCggtc > 1:120581/1‑149 (MQ=255) aTGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtg < 2:1012634/150‑1 (MQ=255) ccTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCCGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCggtcgtc > 1:813201/1‑149 (MQ=255) ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtg > 1:907540/1‑150 (MQ=255) ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtg > 2:936/1‑150 (MQ=255) ccTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggt < 2:867496/149‑1 (MQ=255) cTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCggtcgtcg > 1:149296/1‑148 (MQ=255) cTTCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGGGATGCCTTGACCTAACGGTCCggtcgtcg > 1:233667/1‑148 (MQ=255) cTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCggtggtgg > 1:1053031/1‑150 (MQ=255) ttCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGGGATGCCTTGACCTAACCGTCCggtcgtcgt > 2:630472/1‑150 (MQ=255) tCCATTAAGCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGtt > 2:538377/1‑150 (MQ=255) tCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGtt > 1:840938/1‑150 (MQ=255) cattaagCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGttt < 1:175116/143‑1 (MQ=255) cattaagCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGttt < 1:33123/143‑1 (MQ=255) catcatGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGttt < 2:837379/149‑1 (MQ=255) attaagCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCCGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTc > 2:256411/6‑149 (MQ=255) gCAGCCGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCGACGcc < 2:524918/150‑1 (MQ=255) aGCCGTCGCCCAGGAAGGCGTGGGTGTAGTGGTCGACAATGTCGTGGCACGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATGCCCTGACCCAGCGGACCGGTGGTGGTTTACACACCAg < 2:858597/150‑1 (MQ=255) ccGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCg < 2:577775/150‑1 (MQ=255) ccGTCGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCGACGcccggc < 1:976001/150‑5 (MQ=255) tCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtg < 2:88850/150‑1 (MQ=255) tCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtg < 2:1076468/150‑1 (MQ=255) cGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtg > 2:113877/1‑149 (MQ=255) cGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGgtg > 1:600416/1‑149 (MQ=255) cGCCCAGGAAGGCGTAGGTGTAGTGGTCGACGATTTCGTGGCCGGGGCGGTTGAATTGGCCCGCCAGCGCGCGTTCGGCTATCGCGAAACCCACGGCATTGGTGATGCCTTGACCTAACGGTCCGGTCGTCGTTTCGACGcccggcgca > 2:1067353/1‑142 (MQ=255) ccATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAc > 2:707819/1‑150 (MQ=255) aTGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAAccc < 1:1099729/150‑1 (MQ=255) aaGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACt > 2:623426/1‑150 (MQ=255) ggCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTc > 2:401548/1‑150 (MQ=255) cGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCg < 2:1036743/150‑1 (MQ=255) gtgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGa > 2:306582/1‑150 (MQ=255) gtgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGa < 2:1046925/150‑1 (MQ=255) tgtAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAc > 2:180945/1‑150 (MQ=255) tAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGAccc < 2:898778/150‑1 (MQ=255) gTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg > 1:897947/1‑149 (MQ=255) gTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCg > 2:641093/1‑149 (MQ=255) tGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGa < 2:118689/150‑1 (MQ=255) gTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGt > 1:376704/1‑150 (MQ=255) cGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGttt > 1:276829/1‑150 (MQ=255) gACAATGCCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGttt > 1:782338/1‑149 (MQ=255) aTGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTagag < 1:492641/150‑1 (MQ=255) gTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTg > 2:816631/1‑150 (MQ=255) gTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCa < 1:56450/149‑1 (MQ=255) ggCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCt > 2:475717/1‑150 (MQ=255) gCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCt > 2:915073/1‑149 (MQ=255) cccGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCa < 2:350974/115‑1 (MQ=255) cGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACg > 1:524238/1‑150 (MQ=255) ggACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGCGGCGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACg > 2:44193/1‑149 (MQ=255) cGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAg > 2:512603/1‑140 (MQ=255) ggTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGt > 1:157469/1‑150 (MQ=255) ggTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGt > 1:222265/1‑150 (MQ=255) ttAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttt > 2:748115/1‑150 (MQ=255) ttAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttt < 1:938620/150‑1 (MQ=255) aaaCTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttttt > 1:1085720/1‑150 (MQ=255) aaCTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGttttt < 2:91877/149‑1 (MQ=255) cgccgcCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGGAGCTGACGGAAGTTTTTCAGtt < 1:89326/149‑1 (MQ=255) cgccgcCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGttc < 1:300546/150‑1 (MQ=255) cgccgcCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGt < 2:957520/148‑1 (MQ=255) | TTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTTCGTGGGAGATGCCTTCCATCATGCAGCCGTCGCCCATGAAGGCGTAGGTGTAGTGGTCGACAATGTCGTGGCCCGGACGGTTAAACTGCGCCGCCAGCGTTTTTTCTGCAATCGCCATACCGACTGCGTTGGCAATACCCTGACCCAGCGGACCGGTGGTGGTTTCCACACCAGCGGTGTAACCCACTTCCGGGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTC > CP009273/3074441‑3074739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |