Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,804 T→C 79.2% N189N (AAT→AAC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,8040TC79.2% 611.5 / 163.2 283N189N (AAT→AAC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base T (25/34);  new base C (116/108);  total (141/142)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-05

Only 100 of 283 total aligned reads displayed.

CGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCT  >  CP009273/8658‑8952
                                                                                                                                                  |                                                                                                                                                    
  tgctgcAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGa                                                                                                                                                 <  2:693526/150‑1 (MQ=255)
    ccgctgAACAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCTCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGac                                                                                                                                                >  2:398176/6‑148 (MQ=255)
       cAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATaag                                                                                                                                             <  1:772824/149‑1 (MQ=255)
       cAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATaag                                                                                                                                             <  1:482361/149‑1 (MQ=255)
          aaCAGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACaaga                                                                                                                                            >  1:882715/1‑147 (MQ=255)
            cAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAagag                                                                                                                                       >  1:97231/1‑150 (MQ=255)
             aGCTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAagag                                                                                                                                       >  2:935979/1‑149 (MQ=255)
             aGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGt                                                                                                                                      <  1:36029/150‑1 (MQ=255)
               cTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTAc                                                                                                                                    >  2:970607/1‑150 (MQ=255)
               cTGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTAc                                                                                                                                    <  1:931900/150‑1 (MQ=255)
                tGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACg                                                                                                                                   >  2:512112/1‑150 (MQ=255)
                tGGAAAAAGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACg                                                                                                                                   >  1:689873/1‑150 (MQ=255)
                tGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACg                                                                                                                                   >  1:168201/1‑150 (MQ=255)
                   aaaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGcac                                                                                                                                >  2:786601/1‑148 (MQ=255)
                    aaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                                                                                               <  2:581421/150‑1 (MQ=255)
                    aaaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                                                                                               <  2:222834/150‑1 (MQ=255)
                     aaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCg                                                                                                                              >  1:423075/1‑150 (MQ=255)
                     aaaGAAGGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCg                                                                                                                              <  2:874598/150‑1 (MQ=255)
                      aagaagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTAc                                                                                                                                    >  1:591888/1‑143 (MQ=255)
                       agaagGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCgg                                                                                                                             >  2:343912/1‑149 (MQ=255)
                        gaagGCATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCa                                                                                                                           <  1:713366/150‑1 (MQ=255)
                             cATTAACTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCagaaga                                                                                                                      <  1:289957/150‑1 (MQ=255)
                             cATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCagaaga                                                                                                                      <  1:446101/150‑1 (MQ=255)
                              attaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCag                                                                                                                          >  1:760436/4‑145 (MQ=255)
                               ttaaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCAcc                                                                                                                               <  1:128140/137‑1 (MQ=255)
                                 aaCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCg                                                                                                                  >  1:438124/1‑150 (MQ=255)
                                  aCTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCgg                                                                                                                 >  1:681870/1‑150 (MQ=255)
                                   cTGTAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCggg                                                                                                                <  2:880569/150‑1 (MQ=255)
                                      tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCg                                                                                                              <  1:750261/149‑1 (MQ=255)
                                      tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCg                                                                                                              <  1:241082/149‑1 (MQ=255)
                                      tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGt                                                                                                             <  1:403627/150‑1 (MQ=255)
                                      tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGt                                                                                                             >  1:729675/1‑150 (MQ=255)
                                      tAACCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCATGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCg                                                                                                              <  2:615700/149‑1 (MQ=255)
                                       aaCCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTg                                                                                                            >  2:686537/1‑150 (MQ=255)
                                       aaCCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTg                                                                                                            >  2:657167/1‑150 (MQ=255)
                                        aCCTGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTgg                                                                                                           <  1:217435/150‑1 (MQ=255)
                                          cTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTTTGCGGAAGCTGGCGTGTTCCTGATCTCGCTGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGCGTACGCTCCGGCAGAAGATCCGGGCGTGGtt                                                                                                         >  2:912843/1‑150 (MQ=255)
                                           tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                        >  2:774412/1‑150 (MQ=255)
                                           tGACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGttt                                                                                                        >  1:1069098/1‑150 (MQ=255)
                                            gACGCTGCTGTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTc                                                                                                       >  2:237133/1‑150 (MQ=255)
                                             acgctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTc                                                                                                       <  2:359689/146‑1 (MQ=255)
                                              cccTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTg                                                                                                     <  2:679618/150‑1 (MQ=255)
                                              cccTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTg                                                                                                     >  1:509212/1‑150 (MQ=255)
                                               gctgctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGt                                                                                                    <  1:256692/149‑1 (MQ=255)
                                                   ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcag                                                                                                 <  1:923038/149‑4 (MQ=255)
                                                   ctgTTCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtca                                                                                                  <  1:18171/148‑3 (MQ=255)
                                                      ttCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagcga                                                                                              >  1:617170/1‑143 (MQ=255)
                                                      ttCTCCTTCGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGtcagcga                                                                                              >  1:980355/1‑143 (MQ=255)
                                                              cGCTCAGGCTGGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTGGACTGGTACAAAGCCAACGCCGACAAGAAAGAGTACGCGCCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTAc                                                                                       <  2:1013864/148‑1 (MQ=255)
                                                              cGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTAc                                                                                       <  2:275376/148‑1 (MQ=255)
                                                              cGCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTAc                                                                                       <  1:201374/148‑1 (MQ=255)
                                                              cGCTCAGGCTCGCGCCTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTGGGCCGTCTCCTCGGCTGGTACAAAGCCAACACCGCCAAAAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTAc                                                                                       >  2:724076/1‑148 (MQ=255)
                                                               gCTCAGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAg                                                                                    <  2:1059286/150‑1 (MQ=255)
                                                                   aGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGtact                                                                                <  1:372046/150‑1 (MQ=255)
                                                                   aGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGtact                                                                                <  2:632682/150‑1 (MQ=255)
                                                                   aGGCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGta                                                                                  <  1:1061607/148‑1 (MQ=255)
                                                                    ggCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGtacta                                                                               <  1:851660/150‑1 (MQ=255)
                                                                    ggCTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGtatta                                                                               <  1:638766/150‑1 (MQ=255)
                                                                      cTCGCGCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGtattac                                                                              >  2:420238/1‑149 (MQ=255)
                                                                         gcgCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACaag                                                                           <  1:557314/147‑2 (MQ=255)
                                                                           gCTTGCGCCGAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACaagcag                                                                        <  2:1069098/150‑5 (MQ=255)
                                                                              tgcgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCAc                                                                     >  1:739796/7‑150 (MQ=255)
                                                                                cgccgAAGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACg                                                                    <  1:672529/145‑1 (MQ=255)
                                                                                  cGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGt                                                                  <  2:55805/149‑1 (MQ=255)
                                                                                      aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATg                                                              >  1:392523/1‑149 (MQ=255)
                                                                                      aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                             <  1:786601/150‑1 (MQ=255)
                                                                                      aGCGGGCGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGa                                                             <  1:803169/150‑1 (MQ=255)
                                                                                            cGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGt                                                       >  1:469939/1‑150 (MQ=255)
                                                                                            cGTCTTCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGt                                                       <  1:11947/150‑1 (MQ=255)
                                                                                             gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                      <  2:483017/147‑2 (MQ=255)
                                                                                             gtcttCCTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGtc                                                      >  2:84492/4‑149 (MQ=255)
                                                                                                   cTGATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATg                                                <  1:90854/150‑1 (MQ=255)
                                                                                                     gATTTCGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATggg                                              >  2:450849/1‑150 (MQ=255)
                                                                                                      atttcGCCGTTCGTTGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGgc                                             >  2:6628/4‑150 (MQ=255)
                                                                                                           gCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGgc                                             <  1:569785/145‑1 (MQ=255)
                                                                                                             cgttcgttGGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCt                                      <  2:538919/150‑1 (MQ=255)
                                                                                                              gTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCtt                                     >  2:441435/1‑150 (MQ=255)
                                                                                                                 tgttgGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCg                                  >  2:311892/1‑150 (MQ=255)
                                                                                                                 cgttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                  >  1:889625/2‑150 (MQ=255)
                                                                                                                  gttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCg                                  <  2:326394/149‑1 (MQ=255)
                                                                                                                   ttgGCCGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTa                                <  2:594271/150‑1 (MQ=255)
                                                                                                                       ccGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgt                            <  2:799129/150‑3 (MQ=255)
                                                                                                                        cGTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGc                                       <  2:12074/138‑1 (MQ=255)
                                                                                                                         gTATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttg                          <  2:146128/150‑5 (MQ=255)
                                                                                                                          tATCCTCGACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAAcgttgg                         >  1:1043377/1‑145 (MQ=255)
                                                                                                                              cTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGa                      <  1:343912/149‑1 (MQ=255)
                                                                                                                                 gACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAAtt                  >  1:528554/1‑150 (MQ=255)
                                                                                                                                 gACTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAAtt                  >  1:239275/1‑150 (MQ=255)
                                                                                                                                  aCTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTc                 >  2:202127/1‑150 (MQ=255)
                                                                                                                                   cTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCt                <  1:333376/150‑1 (MQ=255)
                                                                                                                                   cTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCt                <  2:295599/150‑1 (MQ=255)
                                                                                                                                   cTGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCACAAGCTTCCGTa                                >  2:823405/1‑134 (MQ=255)
                                                                                                                                    tGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTg               >  1:136684/1‑150 (MQ=255)
                                                                                                                                    tGGTACAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCACAAGCTTCCGTaa                               <  1:823405/134‑1 (MQ=255)
                                                                                                                                        aCAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc           >  1:847720/1‑150 (MQ=255)
                                                                                                                                        aCAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAAc           >  1:573589/1‑150 (MQ=255)
                                                                                                                                         cAAAGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACt          <  2:906700/150‑1 (MQ=255)
                                                                                                                                          aaaGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTg         <  1:1011811/150‑1 (MQ=255)
                                                                                                                                           aaGCCAACACCGACAAGAAAGAGTACGCACCGGCAGAAGATCCGGGCGTGGTTTCCGTCAGCGAAATCTACGAGTATTACAAGCAGCACGGCTATGAAACCGTCGTGATGGGCGCAAGCTTCCGTAACGTTGGCGAAATTCTGGAACTgg        <  2:760436/150‑1 (MQ=255)
                                                                                                                                                 aTACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCt  <  1:648881/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGTGCTGCAGAACAGCTGGAAAAAGAAGGCATCAACTGTAACCTGACCCTGCTGTTCTCCTTCGCTCAGGCTCGTGCTTGTGCGGAAGCGGGCGTGTTCCTGATCTCGCCGTTTGTTGGCCGTATTCTTGACTGGTACAAAGCGAATACCGATAAGAAAGAGTACGCTCCGGCAGAAGATCCGGGCGTGGTTTCTGTATCTGAAATCTACCAGTACTACAAAGAGCACGGTTATGAAACCGTGGTTATGGGCGCAAGCTTCCGTAACATCGGCGAAATTCTGGAACTGGCAGGCT  >  CP009273/8658‑8952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑