| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 360,129 | T→G | 10.3% | intergenic (‑63/+532) | lacZ ← / ← lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 360,129 | 0 | T | G | 10.3% | 253.5 / 2.2 | 97 | intergenic (‑63/+532) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (46/35); new base G (11/2); total (59/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGA > CP009273/359980‑360255 | cTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGt > 1:996790/1‑150 (MQ=255) cgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTc > 1:81404‑M1/124‑150 (MQ=255) gttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTcc > 1:439180‑M1/123‑150 (MQ=255) tttACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTcc < 1:266889/149‑1 (MQ=255) tttACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCt < 1:85718/150‑1 (MQ=255) tcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGCCCTg < 1:687100‑M1/30‑1 (MQ=255) aaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGc > 1:927152‑M1/119‑149 (MQ=255) aCCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGc < 1:664371/149‑1 (MQ=255) aatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGccc < 2:1010426‑M1/33‑1 (MQ=255) cTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCg < 1:442899/150‑1 (MQ=255) atccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGGGGCCTgcccc < 2:452142‑M1/34‑2 (MQ=255) ccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGcc > 2:169687‑M1/115‑146 (MQ=255) ccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGGCCTGcc < 1:169687‑M1/32‑1 (MQ=255) cgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCa < 1:379871‑M1/37‑1 (MQ=255) tgGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCCGcccccccc > 1:186426/1‑148 (MQ=255) gctcccggcggatttgtcctactcaggagagcgttcaccgaaaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCAc > 2:419140‑M1/113‑150 (MQ=255) ccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCt > 1:368277‑M1/109‑149 (MQ=255) cGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTcc < 1:1020585/150‑1 (MQ=255) ggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCg > 2:98792‑M1/107‑150 (MQ=255) aCATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCgg < 1:724115/150‑1 (MQ=255) cAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGt < 1:101323/150‑1 (MQ=255) cAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCCCCCGGcccg > 2:912662/1‑148 (MQ=255) gCACTTCACCGCTTGCCAGCGGCTTTCCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCtt < 1:906831/150‑1 (MQ=255) gCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCCGCCCTTGcct > 2:423787/1‑148 (MQ=255) gCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCCGCCGCTGCtt > 2:665365/1‑150 (MQ=255) cTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGGGTCCTGGCCCCCCCCCCCCCGGCCCGTGCTTccc > 2:505395/1‑148 (MQ=255) tCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGGCCGCCACCCCCCCGGCCGCTGCTTCGC‑aa > 1:676633/1‑150 (MQ=255) ccGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGCTGCTTCGC‑AAC‑G‑t > 1:126647/1‑150 (MQ=38) cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑tt < 1:581883/150‑1 (MQ=255) ttGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCCCTGCTTCGC‑CAC‑G‑GTCa > 2:498679/1‑149 (MQ=38) ttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATcc > 2:379871‑M1/80‑150 (MQ=255) ttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATcc > 1:948069‑M1/80‑150 (MQ=255) ttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATcc > 1:134017‑M1/80‑150 (MQ=255) tcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGGGTCTTGGCCCCCCCCCCCCCGGCCGTTGCTTCCCCAAC‑g‑ttcaatacc < 2:950180‑M1/72‑5 (MQ=255) ggCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTc < 1:980988/150‑1 (MQ=255) cTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCGCCCCCCCCCCGGCCGTTGCTTCTC‑CAC‑G‑TTCAAAACCGCcccc > 2:5488/1‑150 (MQ=255) acaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCcg > 1:264962‑M1/73‑150 (MQ=255) caaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCcgg > 1:657522‑M1/72‑150 (MQ=255) aCCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCCC‑AAC‑GCTTCAAACCCCCCCCcgc > 1:3175/1‑149 (MQ=37) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCcggc < 1:112397/149‑1 (MQ=255) ccATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCGCCCCCCCCCCGGCCGGTGCTTCGC‑AAC‑G‑CTCAAAACCGCTCCccgcg > 1:968804/1‑150 (MQ=255) cATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCCGGCGCTGCTTCCC‑CAC‑G‑GTTCAATCCCCTccccccgg > 2:264962/1‑145 (MQ=255) acaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCcggcgg > 2:400182‑M1/69‑150 (MQ=255) acaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCcggcgg > 2:76578‑M1/69‑150 (MQ=255) ccAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGCCCCCTCCCCCCCCCCCCCGGCCGTTGTTTCGC‑AAC‑G‑GTCAAAACCCCCCCCCCCGGact > 1:837883/1‑148 (MQ=255) cagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGAttt > 1:950180‑M1/65‑150 (MQ=255) cagagaaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGAGGACTGCCCGCCACCCTCAGGGAAGTTGATTAGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGAttt > 1:3966‑M1/65‑150 (MQ=255) cAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGAtt < 1:776989/149‑1 (MQ=255) aGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTg < 1:835517/150‑1 (MQ=255) aaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGCCGCTGCTTCCC‑AAC‑A‑TTTAAATCCCCTCCCCGCGGGTTTGTCCt < 1:614721‑M1/91‑1 (MQ=255) aaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCt > 1:458259‑M1/59‑149 (MQ=255) aaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTa > 1:274018‑M1/59‑150 (MQ=255) aacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCCCCCGGGCGGTGCTTCGC‑AAA‑G‑TTCAAAACCGCCCCcggcgggttttttcttc < 1:644563‑M1/93‑8 (MQ=255) aacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTAc > 2:482312‑M1/58‑150 (MQ=255) acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACt > 2:687100‑M1/57‑150 (MQ=255) caTCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTc < 2:996790/150‑1 (MQ=255) gaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCa > 1:452142‑M1/55‑150 (MQ=255) tCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCAACTCAg < 2:978393/150‑1 (MQ=255) aaggcccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAgg > 2:499014‑M1/53‑150 (MQ=255) gTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGCGGGCCTCCCCCCCCCCCTTCCGGCCCTTGGTTTTC‑AAC‑C‑ACTAAATACGCACCCGGCGGGTTAGTGCTCCTCTggg > 1:23570/1‑147 (MQ=255) tGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGagag < 1:936530/150‑1 (MQ=255) tGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGagag < 2:158313/150‑1 (MQ=255) cccagtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGagag > 1:246373‑M1/49‑150 (MQ=255) agtctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCGC‑AAC‑C‑TTCAAATCCGCTCCCCGCGGGTTTTTTCTACTCAGGAGAGcgg < 1:318099‑M1/105‑2 (MQ=255) aGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGGCCCGCCCGCCCCCCCCCCCGGCGTTGGTTTGC‑GACGG‑TTCAAAACC‑CTCCCCGCGGGTTTTTTCTTCCCCGGAGAGCgg > 2:658904/1‑149 (MQ=255) tctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTc > 2:487660‑M1/44‑150 (MQ=255) tctttcgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGCCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTc > 2:126009‑M1/44‑150 (MQ=255) ggCTCCGATAATAAGCTGTCAAACATGAGATTTCCGAAGCCCAATACCGGGCAGTTCATGCCGGGCCTCCTCCCCGCCGCCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACACAGGAGAGCGTTCa < 1:324663/149‑1 (MQ=17) gCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 1:863818/150‑1 (MQ=255) gCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAc < 1:505395/150‑1 (MQ=255) cTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCAcc < 1:385816/150‑1 (MQ=255) cgactgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCg > 1:62996‑M1/39‑149 (MQ=255) cGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGa < 2:968760/149‑1 (MQ=255) actgagcctttcgttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGaca > 1:344418‑M1/37‑150 (MQ=255) tAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGacaaacaa < 1:377948/150‑1 (MQ=255) tCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTTGC‑AAA‑G‑TTCAAATCCCCCCCCCGCGGGGTTGTCCTACTCAGGAGAGCGGTCACCCccaaaaaaaaaaaa > 1:98792/1‑143 (MQ=17) gttttatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATaa > 2:610020‑M1/25‑150 (MQ=255) tatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGt > 1:976145‑M1/21‑46 (MQ=255) tatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGt < 2:976145‑M1/26‑1 (MQ=255) tatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTg > 2:345181‑M1/21‑50 (MQ=255) tatttgatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTg < 1:345181‑M1/30‑1 (MQ=255) aCATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACg < 1:924767/150‑1 (MQ=255) gatgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAg > 2:968804‑M1/16‑149 (MQ=255) atgcctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAgg > 1:1010426‑M1/15‑149 (MQ=255) aaTTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGcc < 1:154389/150‑1 (MQ=255) cctggggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGccc > 2:325786‑M1/12‑149 (MQ=255) cGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGCCGGTGCTTCGC‑AAC‑G‑TTTAAATCCCCTCCCCGCGGGTTTGTCCTTCTCAGGGGAGCGGTCACCCACAAAAAACAAAAAAAACGAAAAGCCCAggc > 1:400182/1‑148 (MQ=14) gggaattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt > 2:186426‑M1/8‑150 (MQ=255) gaaaattAGTTCCTGGCAGTTTAGGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt > 2:114582‑M1/8‑150 (MQ=255) gATCCCCAATTCCTGGCAGTTTATGGCGGGGGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCt < 2:837883/150‑1 (MQ=38) gATCCCCAATTCCTGGCAGTTTATGGCGGGGGGCCTGCCCGCCCCCCCCCCGGCCGTTGCTTCGC‑CAC‑G‑GTCAAAAACGCCCCCCGCGGGTTTGTTCCACTCCGGAGAGCGGTCACCCACAAAAAACAGAAAAAAAGAAAAAGCCAGTCt > 2:368620/1‑150 (MQ=255) aTCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCGGTGCTTCGC‑AAA‑G‑GTCAAATCCCCCCCCCGCGGGTTTGTCCTACTCAGGAGAGCGGTCACCGCCAAACAACAAATAAAAAGAAAAGccccatcct > 1:76578/1‑144 (MQ=11) gaattAATTCCGGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCttt > 2:596963‑M1/6‑150 (MQ=255) ccccAATTCCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCCTTGCTTTGC‑AAC‑G‑CTCCAATCCCCCCCCCGCGGGTTTGTTCTTCCCCGGAGGGGGGTCACCGACAAACAAAAGATAAAAAAAAAAGCCCACTCTTTc > 2:246373/1‑150 (MQ=2) aattaaTTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCCGTGCTTCTC‑CAA‑G‑TTCAAAATCCCCCCCCGCGGGTTTTTTCTACTCCGGGGAGCGGTCACCGCCAAACAACAAAAAAAAAAAAAAGACCAGACTTTc > 2:164473/5‑150 (MQ=2) aattAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTc > 1:1011971‑M1/5‑150 (MQ=255) cccAATTCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCGGTGCTTCGC‑AAC‑G‑TTCAAAACCCCTCCCCGCGGGGTTTTTCTACTCAGGGGAGCGTTCACCCCCAAAAAACAAATAAAAAAAAAAGCCCAGGCTTTc > 1:308148/1‑149 (MQ=2) taaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGGTTCTTCGC‑AAC‑G‑TTCAAAACCCCCCCCCGCGGGTTTTTCCTTCTCCAGAGAGCGTTCCCCCACAAACAACAGAAAAAAAGAAAAGCCCACGCTTTCCAc > 1:968443/2‑150 (MQ=2) aaTTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGAc < 1:423787/149‑1 (MQ=32) ttCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGa < 1:5488/150‑1 (MQ=31) | CTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCCGATGATAAGCTGTCAAACATGAGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AAC‑G‑TTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGA > CP009273/359980‑360255 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |