Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,230,349 T→C 11.1% intergenic (‑176/‑45) nhaB ← / → fadR sodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,230,3490TC11.1% 125.7 / 8.1 45intergenic (‑176/‑45)nhaB/fadRsodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (24/16);  new base C (2/3);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01

AATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACG  >  CP009273/1230202‑1230497
                                                                                                                                                   |                                                                                                                                                    
aaTGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTat                                                                                                                                                    <  2:577186/150‑1 (MQ=255)
 atgatgTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTat                                                                                                                                                    >  2:910688/1‑149 (MQ=255)
   gatgTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTatgat                                                                                                                                                 >  2:858280/1‑150 (MQ=255)
     tgTTTTTATGTTTAAACGCAAAGATAAACGGTCAGGCAGGAGTGAGGCAAGTCATGATAGTCAAGGGGAAAGAGATGAGGAAAATGAAGCCTTGATCACTTATTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTAtgatga                                                                                                                                               <  1:218266/150‑1 (MQ=255)
      gTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAg                                                                                                                                              >  2:43699/1‑150 (MQ=255)
           tATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCaa                                                                                                                                         >  2:195381/1‑150 (MQ=255)
                  aaaCGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGtt                                                                                                                                  >  2:932302/1‑150 (MQ=255)
                     cGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTg                                                                                                                               >  1:417189/1‑150 (MQ=255)
                        aaaGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCtg                                                                                                                            >  2:958246/1‑150 (MQ=255)
                          aGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCtgtg                                                                                                                          >  1:656157/1‑150 (MQ=255)
                               aaCGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTa                                                                                                                       <  1:957470/148‑1 (MQ=255)
                               aaCGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTa                                                                                                                       <  1:900287/148‑1 (MQ=255)
                                      aggcaggAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAAtct                                                                                                              >  1:248990/1‑150 (MQ=255)
                                                 aGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCa                                                                                                   <  2:12287/150‑1 (MQ=255)
                                                       gTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAgg                                                                                             >  2:294433/1‑150 (MQ=255)
                                                        tCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGgc                                                                                            <  1:858280/150‑1 (MQ=255)
                                                              tAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTTTTTTTGTTTGCTATCAGCGTAGGTAGCCCTCTGGTATTGTTAGTCCCACCTTGTTTTTCCGTGTTATGGAAATTCTAATTTTGTCATTAAGGCGCAAAg                                                                                      >  2:414380/1‑150 (MQ=255)
                                                              tAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAg                                                                                      <  1:910898/150‑1 (MQ=255)
                                                                     ggggAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCcggcg                                                                               <  1:439525/150‑1 (MQ=255)
                                                                          aaGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGttt                                                                          >  2:740134/1‑150 (MQ=255)
                                                                          aaGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGttt                                                                          <  2:636540/150‑1 (MQ=255)
                                                                             agaTGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTcgc                                                                       >  2:283954/1‑150 (MQ=255)
                                                                              gaTGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTcgcg                                                                      >  1:479225/1‑150 (MQ=255)
                                                                                     aaaaTGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAagag                                                                >  2:354819/1‑149 (MQ=255)
                                                                                      aaaTGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTa                                                              >  2:346691/1‑150 (MQ=255)
                                                                                            aGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGAACattat                                                        >  2:313473/1‑150 (MQ=255)
                                                                                                      ccTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATc                                              >  1:673127/1‑150 (MQ=255)
                                                                                                       cTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCt                                             >  1:1004362/1‑150 (MQ=255)
                                                                                                          tttCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGATTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTgg                                           >  2:595623/1‑149 (MQ=255)
                                                                                                           ttcttcTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGaa                                         >  1:358224/1‑150 (MQ=255)
                                                                                                           ttcttcTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGa                                          <  2:131914/149‑1 (MQ=255)
                                                                                                            tcttctTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGaa                                         <  2:479225/149‑1 (MQ=255)
                                                                                                             cttcttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAAt                                        <  2:631525/149‑1 (MQ=255)
                                                                                                             cttcttTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAAt                                        <  1:414380/149‑1 (MQ=255)
                                                                                                                   tttGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCg                                   <  1:43699/148‑1 (MQ=255)
                                                                                                                    ttGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCtt                                >  1:178731/1‑150 (MQ=255)
                                                                                                                     tGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtc                               >  2:799408/1‑150 (MQ=255)
                                                                                                                           cTATCAGCGTAGTTAGCCCTCTGGCATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTtccctc                           <  1:283954/148‑1 (MQ=255)
                                                                                                                              tCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCgg                       <  2:701731/149‑1 (MQ=255)
                                                                                                                                aGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGa                     <  2:457332/149‑1 (MQ=255)
                                                                                                                                 gCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACt                   <  1:910688/150‑1 (MQ=255)
                                                                                                                                          gCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGcc           <  1:366407/149‑1 (MQ=255)
                                                                                                                                            ccTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTTTTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGTCCg         >  1:187560/1‑149 (MQ=255)
                                                                                                                                                tGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGaa    >  1:245060/1‑150 (MQ=255)
                                                                                                                                                  gTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTTGCGGAAGAGGACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACg  >  1:438989/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATGATGTTTTTATGTTTAAACGCAAAGCTTAACGGTCAGGCAGGAGTGAGGCAAGTCTTGATAGTCAAGGGGAAAGAGATGCGGAAAATGAAGCCTTGATCCCTTTTTCTTCTTTTTGTCTGCTATCAGCGTAGTTAGCCCTCTGGTATGATGAGTCCAACTTTGTTTTGCTGTGTTATGGAAATCTCACTATGGTCATTAAGGCGCAAAGCCCGGCGGGTTTCGCGGAAGAGTACATTATTGAAAGTATCTGGAATAACCGCTTCCCTCCCGGGACTATTTTGCCCGCAGAACG  >  CP009273/1230202‑1230497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑