Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,977,727 A→C 100% I275R (ATA→AGA)  araG ← fused L‑arabinose transporter subunits of ABC superfamily: ATP‑binding components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,977,7270AC100.0% 173.5 / NA 48I275R (ATA→AGA) araGfused L‑arabinose transporter subunits of ABC superfamily: ATP‑binding components
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (29/19);  total (29/19)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCG  >  CP009273/1977580‑1977873
                                                                                                                                                   |                                                                                                                                                  
ttACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTTCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTTCCAGACCAAACAGCCCCACAATTTCACCACTGCGAACCCCCCGActtctt                                                                                                                                                  >  2:54574/1‑150 (MQ=255)
ttACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACAAGCCTTTCATTAATTCGCTACGCCCCGCCCCCACCAGACCCAACAACCCAACAATTTCACCACTGCGAAACCCCCGActtctt                                                                                                                                                  >  1:1038895/1‑150 (MQ=255)
   cGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTgg                                                                                                                                                <  1:906844/149‑1 (MQ=255)
       taacgatcgGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTac                                                                                                                                           <  1:540795/147‑1 (MQ=255)
           gatcgGCTGTTGGTCGATATAAACCTGACCGGCGGTGAATTGCGTCCCTCCAAACAATCCTTTTATTTATTTGCTACGCCCCCCCCCTACCTAACCAAACACCCCAAAAAATTCACCTCTTCGAACCGCCTCTCTTCTTTACGTacgcac                                                                                                                                       >  2:592705/1‑150 (MQ=255)
                       gTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCttt                                                                                                                           <  2:731792/150‑1 (MQ=255)
                              taAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCa                                                                                                                    <  1:978265/150‑1 (MQ=255)
                                       ccGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACg                                                                                                            >  2:551363/1‑149 (MQ=255)
                                        cggcggTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCCCTGCGAACCGCCAGACTTCTTTGCGTACGCACGCCTGGTGCTTTCACAGCATCCAGACg                                                                                                            >  1:998247/1‑148 (MQ=255)
                                           cggTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGgc                                                                                                       >  2:617865/1‑150 (MQ=255)
                                             gTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGgcgc                                                                                                     >  2:291073/1‑150 (MQ=255)
                                              tGATTTGCGCCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGct                                                                                                    <  2:40687/150‑1 (MQ=255)
                                                aTTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGctcc                                                                                                  <  2:430659/150‑1 (MQ=255)
                                                  ttGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGctcctc                                                                                                >  2:205568/1‑150 (MQ=255)
                                                    gCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCg                                                                                               <  2:247461/149‑1 (MQ=255)
                                                    gCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGc                                                                                              >  2:503909/1‑150 (MQ=255)
                                                       tCCCGCCAAACATGCCTTTCATTAATCCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCAt                                                                                           <  1:30893/150‑1 (MQ=255)
                                                          cGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAAc                                                                                        >  2:219749/1‑150 (MQ=255)
                                                              aaaCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAcg                                                                                    >  2:56116/1‑150 (MQ=255)
                                                               aaCATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTAcgc                                                                                   >  1:736786/1‑150 (MQ=255)
                                                                     ccTTTCATTAATTCGCTACGCCTCGCCCCTACCAGACCAAACAGCCCAATAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACTCACCCCTGGTGCTTTCACAGCATCAAGACGTAAGCGCTTCTCGCCATAACTACGCGGTTGc                                                                             >  2:912483/1‑150 (MQ=255)
                                                                              aaTTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCg                                                                       >  1:876202/1‑147 (MQ=255)
                                                                               aTTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCg                                                                       >  1:554755/1‑146 (MQ=255)
                                                                                ttCGCTACCCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGat                                                                  <  2:922982/150‑1 (MQ=255)
                                                                                   gCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGatat                                                                <  1:956365/149‑1 (MQ=255)
                                                                                    cTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCg                                                              >  1:772415/1‑150 (MQ=255)
                                                                                       cgccccgcccCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGc                                                             <  2:771041/148‑1 (MQ=255)
                                                                                       cgccccgcccCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCa                                                           <  1:326097/150‑1 (MQ=255)
                                                                                            cgccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCACCAATGTc                                                      <  1:525332/150‑1 (MQ=255)
                                                                                              ccccTACCAGACCAAACAGCCCAACAATTTCACCACCGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTATGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTcgc                                                    >  2:432069/1‑150 (MQ=255)
                                                                                               cccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTcgcg                                                   >  2:995790/1‑150 (MQ=255)
                                                                                                 cTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGcc                                                 >  2:576306/1‑150 (MQ=255)
                                                                                                 cTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGcc                                                 <  1:205568/150‑1 (MQ=255)
                                                                                                   aCCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGccc                                                >  2:495881/1‑149 (MQ=255)
                                                                                                    ccAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGa                                              >  1:953399/1‑150 (MQ=255)
                                                                                                          cAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCg                                        <  2:1039285/150‑1 (MQ=255)
                                                                                                             aCAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCt                                     <  2:384801/150‑1 (MQ=255)
                                                                                                                gCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCa                                  >  1:975257/1‑150 (MQ=255)
                                                                                                                gCCCAACAATTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCa                                  <  2:772415/150‑1 (MQ=255)
                                                                                                                   caacaaTTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCAc                                 >  2:49481/1‑148 (MQ=255)
                                                                                                                      caaTTTCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAgcg                            >  1:594265/1‑150 (MQ=255)
                                                                                                                         tttCACCACTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGcgt                         >  2:505822/1‑150 (MQ=255)
                                                                                                                              ccacTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTgg                    <  1:291073/150‑1 (MQ=255)
                                                                                                                               cacTGCGAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGt                   >  2:547368/1‑150 (MQ=255)
                                                                                                                                     gAACCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACct             >  2:891190/1‑150 (MQ=255)
                                                                                                                                      aaCCGCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACctg            >  1:91703/1‑150 (MQ=255)
                                                                                                                                          gCCAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACctgctg         >  2:124240/1‑149 (MQ=255)
                                                                                                                                            cAGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCa       >  1:945135/1‑149 (MQ=255)
                                                                                                                                             aGACTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCata     >  1:942608/1‑150 (MQ=255)
                                                                                                                                               aCTTCTTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATc   <  2:1038895/150‑1 (MQ=255)
                                                                                                                                                cttcttGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCg  <  2:876202/150‑1 (MQ=255)
                                                                                                                                                cttcttGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCg  <  1:617865/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCG  >  CP009273/1977580‑1977873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑