Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,583,599 A→T 5.6% Y225N (TAT→AAT)  ugpA ← glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,583,5990AT 5.6% 249.5 / 5.1 72Y225N (TAT→AAT) ugpAglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (30/37);  new base T (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

CAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAA  >  CP009273/3583453‑3583743
                                                                                                                                                  |                                                                                                                                                
cAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATacac                                                                                                                                               >  1:357191/1‑150 (MQ=255)
 aaCACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATacac                                                                                                                                               >  1:676148/1‑149 (MQ=255)
   caccacCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAg                                                                                                                                            >  2:14186/1‑150 (MQ=255)
          gACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGACGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCAc                                                                                                                                      >  2:627583/1‑149 (MQ=255)
            cTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTAc                                                                                                                                   <  1:591566/150‑1 (MQ=255)
                  ggCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACcagcag                                                                                                                             <  2:963400/150‑1 (MQ=255)
                    cAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGga                                                                                                                           <  1:147469/150‑1 (MQ=255)
                       aCGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGgaaga                                                                                                                        <  1:196862/150‑1 (MQ=255)
                         gAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGaaa                                                                                                                      <  1:524034/150‑1 (MQ=255)
                         gAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGaaa                                                                                                                      >  2:63432/1‑150 (MQ=255)
                             ccAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCa                                                                                                                  <  2:466894/150‑1 (MQ=255)
                               aGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCAcc                                                                                                                <  1:341887/150‑1 (MQ=255)
                               aGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCAcc                                                                                                                >  2:153349/1‑150 (MQ=255)
                                gATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCg                                                                                                               <  1:694424/150‑1 (MQ=255)
                                   ccAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCgggg                                                                                                            >  1:153496/1‑150 (MQ=255)
                                    cAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCgggg                                                                                                            <  1:470977/149‑1 (MQ=255)
                                    cAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGc                                                                                                           <  2:523500/150‑1 (MQ=255)
                                     aGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGTGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGc                                                                                                           >  1:578849/1‑149 (MQ=255)
                                      gTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGa                                                                                                         >  2:1023982/1‑150 (MQ=255)
                                       tCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGAt                                                                                                        <  2:821304/150‑1 (MQ=255)
                                        ccGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGAt                                                                                                        >  1:844689/1‑149 (MQ=255)
                                         cGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATaa                                                                                                      <  2:314534/150‑1 (MQ=255)
                                         cGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATa                                                                                                       <  2:169899/149‑1 (MQ=255)
                                           gTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGc                                                                                                    <  2:687841/150‑1 (MQ=255)
                                            tAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCg                                                                                                   <  2:706939/150‑1 (MQ=255)
                                              aaaCCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCgg                                                                                                  <  1:63432/149‑1 (MQ=255)
                                                  cTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcg                                                                                             >  2:749517/1‑150 (MQ=255)
                                                     cgcgGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAgcgc                                                                                            <  1:515379/148‑1 (MQ=255)
                                                             aTCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTa                                                                                     >  1:318263/1‑147 (MQ=255)
                                                                 tataAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                                                              >  2:30598/1‑150 (MQ=255)
                                                                 tataAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                                                              >  2:888882/1‑150 (MQ=255)
                                                                  ataAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgc                                                                              <  2:645615/149‑1 (MQ=255)
                                                                   taAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgcgg                                                                            >  2:565996/1‑150 (MQ=255)
                                                                     aaTCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgcggcg                                                                          <  2:964302/150‑1 (MQ=255)
                                                                     aaTCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgcggc                                                                           <  2:373312/149‑1 (MQ=255)
                                                                     aaTCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgcggc                                                                           <  1:359156/149‑1 (MQ=255)
                                                                     aaTCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAgcggc                                                                           >  2:198011/1‑149 (MQ=255)
                                                                      aTCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGc                                                                                                 >  2:323085/1‑126 (MQ=255)
                                                                       tCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCa                                                                                                <  1:323085/126‑1 (MQ=255)
                                                                        cAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                                                                       >  1:5166/1‑150 (MQ=255)
                                                                        cAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAAt                                                                       >  1:642889/1‑150 (MQ=255)
                                                                               gtggtgGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCt                                                                <  1:506540/150‑1 (MQ=255)
                                                                               gtggtgGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCt                                                                >  2:381646/1‑150 (MQ=255)
                                                                               gtggtgGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGAGTCGAAAAAGGCATACACCAGTTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCt                                                                >  2:888110/1‑150 (MQ=255)
                                                                                  gtggCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGc                                                              <  2:449641/149‑1 (MQ=255)
                                                                                       cTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTc                                                        >  1:685043/1‑150 (MQ=255)
                                                                                          aaCCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGAt                                                     >  2:697795/1‑150 (MQ=255)
                                                                                            ccGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATgg                                                   <  2:357191/150‑1 (MQ=255)
                                                                                              ggCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCt                                                 >  2:291807/1‑150 (MQ=255)
                                                                                                cccGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGc                                               <  1:761616/150‑1 (MQ=255)
                                                                                                    ccgGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCt                                           >  1:568807/1‑150 (MQ=255)
                                                                                                      ggACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATGCACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTc                                         <  2:863120/150‑1 (MQ=255)
                                                                                                      ggACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTc                                         <  2:873876/150‑1 (MQ=255)
                                                                                                         cGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGAt                                      <  2:981580/150‑1 (MQ=255)
                                                                                                          gTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATc                                     <  1:1037585/150‑1 (MQ=255)
                                                                                                                   tCGATCACCGGGAAGGAGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACgg                            >  2:538700/1‑150 (MQ=255)
                                                                                                                     gttCACCGGGAAGGTGTCGAAGAAGGCATTCACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACggg                           >  1:511759/3‑149 (MQ=255)
                                                                                                                     gATCACCGGGAAGGTGTCGAAGAAGGCATTCACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACggg                           >  1:511754/1‑149 (MQ=255)
                                                                                                                      aTCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACgggg                          >  2:520346/1‑149 (MQ=255)
                                                                                                                       tCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGaa                        <  1:99223/150‑1 (MQ=255)
                                                                                                                         aCCGGGAAGGTGTCGAAGAAGGCATTCACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATg                      <  2:511759/150‑1 (MQ=255)
                                                                                                                         aCCGGGAAGGTGTCGAAGAAGGCATTCACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATg                      <  2:511754/150‑1 (MQ=255)
                                                                                                                         aCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATg                      <  2:676148/150‑1 (MQ=255)
                                                                                                                         aCCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATg                      <  1:843692/150‑1 (MQ=255)
                                                                                                                           cGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGa                    >  2:897376/1‑150 (MQ=255)
                                                                                                                             ggAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGAtt                  >  2:404404/1‑150 (MQ=255)
                                                                                                                                 ggTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAg              <  1:627583/150‑1 (MQ=255)
                                                                                                                                     tCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGcgcg          <  1:749517/150‑1 (MQ=255)
                                                                                                                                       gaagaaGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCgg         <  1:1023982/149‑1 (MQ=255)
                                                                                                                                        aagaagGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCa       >  2:477863/1‑150 (MQ=255)
                                                                                                                                         agaagGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCa       >  1:551021/1‑149 (MQ=255)
                                                                                                                                         agaagGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCa       >  1:942829/1‑149 (MQ=255)
                                                                                                                                           aagGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATag    <  2:844689/150‑1 (MQ=255)
                                                                                                                                             ggCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATagaa  <  1:198011/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CAACACCACCGACTGTGCGGCAGACGAAGCCAGATCCAGTCCGGTAAAACCTTCGCGGTAGATCTTATAAATCAGCGTCGTGGTGGCCTGAACCGGCCCGCCGGACGTGGCGGCGTCGATCACCGGGAAGGTGTCGAAGAAGGCATACACCAGGTTCACTACCAGCAGGAAGAAACTCACCGGGGCGATAAGCGGCAGCGCAATCTTAAAGAAGCGGCGAATCGGCCCTGCACCGTCGATGGCTGCGGCTTCGATCAACGAACGGGGAATGGATTGCAGCGCGGCATAGAA  >  CP009273/3583453‑3583743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑