Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,729,296 A→C 100% K320Q (AAG→CAG)  xylR → xylose divergent operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,729,2960AC100.0% 190.7 / NA 54K320Q (AAG→CAG) xylRxylose divergent operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/26);  total (28/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATA  >  CP009273/3729151‑3729438
                                                                                                                                                 |                                                                                                                                              
cATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCg                                                                                                                                            >  2:943004/1‑150 (MQ=255)
   tGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGttt                                                                                                                                         >  1:873562/1‑150 (MQ=255)
     aaCGGCCCTAAAAAGTTTACCGCTCGCTGCCCGACCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTa                                                                                                                                       <  2:78621/150‑1 (MQ=255)
            cTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGATCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAaagaa                                                                                                                                  >  1:965906/1‑148 (MQ=255)
                aCAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAggtg                                                                                                                            >  1:666188/1‑150 (MQ=255)
                   gtttACCGCTCGCTGCCCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAggtgggt                                                                                                                         <  2:1003196/148‑1 (MQ=255)
                     ttATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAggtgggtg                                                                                                                        >  1:843547/1‑149 (MQ=255)
                              gctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAAccatcca                                                                                                              >  1:479036/1‑150 (MQ=255)
                              gctGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAAccatcca                                                                                                              >  1:597490/1‑150 (MQ=255)
                              gcagACCGTCCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAAccatcca                                                                                                              <  2:524602/147‑1 (MQ=255)
                                 gACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgc                                                                                                           >  2:838057/1‑150 (MQ=255)
                                  cccctccccGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatg                                                                                                            <  2:434224/142‑1 (MQ=255)
                                  cccGCTCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATccatgcc                                                                                                          <  2:966954/149‑1 (MQ=255)
                                      ctCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCATTCTGGCGCAGCGTTTTAAAGAAGAGGGGGGTGATATCATCCATGCCACGa                                                                                                      >  2:116612/2‑150 (MQ=255)
                                           gccgTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCAt                                                                                                 <  2:418390/150‑1 (MQ=255)
                                              gTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGcc                                                                                              <  2:1012522/150‑1 (MQ=255)
                                                tATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGcc                                                                                              >  1:613645/1‑148 (MQ=255)
                                                  ttCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCgag                                                                                           >  2:796947/1‑149 (MQ=255)
                                                     aGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGc                                                                                       >  1:904479/1‑150 (MQ=255)
                                                      ggCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCt                                                                                      >  2:507018/1‑150 (MQ=255)
                                                         cATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGga                                                                                   <  2:597490/150‑1 (MQ=255)
                                                          aTGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGgag                                                                                  <  1:897621/150‑1 (MQ=255)
                                                            gCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGgaga                                                                                 >  1:834859/1‑149 (MQ=255)
                                                              aTTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAg                                                                              <  1:838057/150‑1 (MQ=255)
                                                                  cATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgcgcg                                                                          <  1:943004/150‑1 (MQ=255)
                                                                   aTTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgcgcgc                                                                         <  1:496065/150‑1 (MQ=255)
                                                                   aTTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAgcgcg                                                                          >  2:638401/1‑149 (MQ=255)
                                                                      cGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGt                                                                      >  1:753215/1‑150 (MQ=255)
                                                                          gtCACGCCTGTAAAGGGATTAGAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTctg                                                                   <  2:920034/148‑1 (MQ=255)
                                                                             aCGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGAGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTctgctg                                                                <  2:733006/149‑1 (MQ=255)
                                                                              cGCATGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGAt                                                              <  2:873562/150‑1 (MQ=255)
                                                                                    tAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCaa                                                         <  2:168612/149‑1 (MQ=255)
                                                                                    tAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAac                                                        >  1:931306/1‑150 (MQ=255)
                                                                                    tAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAac                                                        >  1:309246/1‑150 (MQ=255)
                                                                                       aGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAaccac                                                     <  2:664777/150‑1 (MQ=255)
                                                                                          gATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCtt                                                  >  1:200469/1‑150 (MQ=255)
                                                                                            ttAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGt                                                <  2:658945/150‑1 (MQ=255)
                                                                                                 gTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGAt                                            >  1:908350/1‑149 (MQ=255)
                                                                                                    gATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt                                        >  2:579568/1‑150 (MQ=255)
                                                                                                     aTCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt                                        >  1:815477/1‑149 (MQ=255)
                                                                                                     aTCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATg                                       <  2:479036/150‑1 (MQ=255)
                                                                                                      tCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAAt                                        >  2:240972/1‑148 (MQ=255)
                                                                                                      tCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATga                                      <  1:102775/150‑1 (MQ=255)
                                                                                                             cTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCg                               >  2:6555/1‑150 (MQ=255)
                                                                                                                gATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGc                              <  2:143334/148‑1 (MQ=255)
                                                                                                                gATGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGc                              <  2:904479/148‑1 (MQ=255)
                                                                                                                 aTGCGGTCGGGATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCaaa                           <  1:894661/150‑1 (MQ=255)
                                                                                                                          ggATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGtt                  <  1:750354/150‑1 (MQ=255)
                                                                                                                           gATCTCGCGCTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTa                 >  1:773033/1‑150 (MQ=255)
                                                                                                                                 gcgcTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCAt            <  1:240972/149‑1 (MQ=255)
                                                                                                                                    cTCCAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCt        >  1:373190/1‑150 (MQ=255)
                                                                                                                                       cAATCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGc      >  2:91687/1‑149 (MQ=255)
                                                                                                                                        aaTCTTGAGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCaa    <  1:638401/150‑1 (MQ=255)
                                                                                                                                         ctCTTGCGCAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGCGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGCTCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAat   >  2:34959/2‑150 (MQ=255)
                                                                                                                                              gagcAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAata  <  1:6555/146‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CATTGAACGGCGCTCAACAGATTATCGCTCGCTGACCGATCCCGCCGTTATTCAGGCCATGCATTACATTCGTAATCACGCCTGTAAAGGGATTAAAGTGGATCAGGTACTGGATGCGGTCGGGATCTCGCGCTCCAATCTTGAGAAGCGTTTTAAAGAAGAGGTGGGTGAAACCATCCATGCCATGATTCATGCCGAGAAGCTGGAGAAAGCGCGCAGTCTGCTGATTTCAACCACCTTGTCGATCAATGAGATATCGCAAATGTGCGGTTATCCATCGCTGCAATA  >  CP009273/3729151‑3729438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑