Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,255 C→T 29.1% T6T (ACC→ACT)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,2550CT29.1% 128.1 / 76.3 93T6T (ACC→ACT) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base C (36/30);  new base T (12/15);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-01

TGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTG  >  CP009273/8107‑8403
                                                                                                                                                    |                                                                                                                                                    
tgtCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCt                                                                                                                                                     <  2:588562/150‑1 (MQ=255)
     gAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCtt                                                                                                                                                <  1:61454/150‑1 (MQ=255)
      aaaTCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGTGCTGACGTTGCGTCCTGATATAATTAGGGCCGACCGGTTTTTTCCCCCTCACAAGCTGTTTTAAGAGGAATACTATCATGACGGACAAATTGACCTCCCttt                                                                                                                                               >  2:975754/1‑149 (MQ=255)
        aTCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGGTATCATCAGGGCAGACCGGGTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGt                                                                                                                                             >  2:667214/1‑150 (MQ=255)
             aaaCAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTa                                                                                                                                        <  1:660055/150‑1 (MQ=255)
              aaCAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTa                                                                                                                                        >  1:519727/1‑149 (MQ=255)
                  gTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTAcacca                                                                                                                                   <  1:389418/150‑1 (MQ=255)
                      cGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAAATGACCTCCCTTCGTCAGTACACCACCg                                                                                                                                >  1:893918/1‑149 (MQ=255)
                       gCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACaccacc                                                                                                                                 <  1:467866/147‑1 (MQ=255)
                        ccTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAATAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAg                                                                                                                             >  1:1000076/1‑150 (MQ=255)
                         cTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTTCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCCTACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCCTAGt                                                                                                                            >  1:708670/1‑150 (MQ=255)
                         cTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGt                                                                                                                            <  2:793423/150‑1 (MQ=255)
                           ggCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTgg                                                                                                                          >  1:90980/1‑150 (MQ=255)
                           ggCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTgg                                                                                                                          >  1:396905/1‑150 (MQ=255)
                            gCGATAACCGTCTTGTCGGCGTTTGCGCTGACGTTACGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAGCAAGCTGTTTAAAGAGAAATACTATCATGACGTACAAATTGACCAAGCTTCGTCAGTACACGGCCGTAGTg                                                                                                                           <  2:163907/148‑1 (MQ=255)
                              gATAACCGTCTTGTCGGCGGTTTCGCTGACGTTGCGTCGTGTTATTTTTTTTGCTTTCCGGTTTCTTTCCCCTTACAAGCTTTTTTAATAGAAATTCTTTCATTTCGGTCAAATTTACCTCCCTTCTTCATTACACCACCGTAGTGGCCg                                                                                                                       >  1:206007/1‑150 (MQ=255)
                               aTAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGGTATCATCCGGGCAGACCGGTTACATCCCCCTAACAAACTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGa                                                                                                                      >  2:538235/1‑150 (MQ=255)
                                tAACCGTCTTGTCGGCGGCTCTGCTGCCGCTGCGTCCTGATATCACCAGGGGAGACCGGTTTCCTCCCCCTAAAAAGCTGTTTTAAGAGAAATACTATCATGACGGACAAATTGACCTTCCCTCGCCAGGACACCACCGAAGTGGCCgc                                                                                                                      >  2:987542/1‑148 (MQ=255)
                                  aCCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGacac                                                                                                                   <  2:780705/150‑1 (MQ=255)
                                  aCCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGaca                                                                                                                    <  2:147352/149‑1 (MQ=255)
                                     gTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTgg                                                                                                                <  2:350647/150‑1 (MQ=255)
                                                ggTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCgg                                                                                                     <  2:582330/150‑1 (MQ=255)
                                                          aCGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAg                                                                                             <  1:363010/148‑1 (MQ=255)
                                                                cgtcgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATc                                                                                      >  2:132322/1‑149 (MQ=255)
                                                                cgtcgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATc                                                                                      >  1:62772/1‑149 (MQ=255)
                                                                   cgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAAcc                                                                                  >  1:954190/1‑150 (MQ=255)
                                                                   cgtGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAAcc                                                                                  >  2:75391/1‑150 (MQ=255)
                                                                     tGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCg                                                                                 >  2:378573/1‑149 (MQ=255)
                                                                     tGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGc                                                                                <  2:917768/150‑1 (MQ=255)
                                                                       atatCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAg                                                                              <  1:795639/150‑1 (MQ=255)
                                                                             tcaGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGcc                                                                        <  1:83526/150‑1 (MQ=255)
                                                                                   cAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaacc                                                                  <  1:23789/150‑1 (MQ=255)
                                                                                    aGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaacc                                                                  >  2:154419/1‑149 (MQ=255)
                                                                                    aGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaacc                                                                  >  2:789816/1‑149 (MQ=255)
                                                                                      aCCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCAcaaccaac                                                               >  1:596127/1‑150 (MQ=255)
                                                                                       ccGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACaaccaacc                                                              <  1:862905/150‑1 (MQ=255)
                                                                                          gTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCATAACCAACCCtt                                                           >  1:68190/1‑150 (MQ=255)
                                                                                              cATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGACGCCACAACCAACCCTtctct                                                       >  2:108836/1‑150 (MQ=255)
                                                                                               aTCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTtctct                                                       >  2:287614/1‑149 (MQ=255)
                                                                                                tCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCa                                                     >  2:311304/1‑150 (MQ=255)
                                                                                                 cccccTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCAt                                                    <  2:206007/150‑1 (MQ=255)
                                                                                                  ccccTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCAtt                                                   >  1:264520/1‑150 (MQ=255)
                                                                                                    ccTAACAAGCTGTTTAAATAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCt                                                 <  2:1000076/150‑1 (MQ=255)
                                                                                                       aaCAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACa                                                                                                           >  1:559161/1‑89 (MQ=255)
                                                                                                        aCAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACAt                                                                                                          <  2:559161/89‑1 (MQ=255)
                                                                                                                tttAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcg                                       >  2:612642/1‑148 (MQ=255)
                                                                                                                 ttAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAAcgcagcg                                       >  2:758/1‑147 (MQ=255)
                                                                                                                     agagAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTc                                >  2:979953/1‑150 (MQ=255)
                                                                                                                      gagaAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTcc                               <  2:417583/150‑1 (MQ=255)
                                                                                                                      gagaAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTcc                               <  1:287614/150‑1 (MQ=255)
                                                                                                                        gaAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCg                              >  1:250908/1‑149 (MQ=255)
                                                                                                                         aaaTACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGa                            <  2:304990/150‑1 (MQ=255)
                                                                                                                            acacaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTa                         <  2:643746/143‑1 (MQ=255)
                                                                                                                            acacaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTa                         <  1:859845/143‑1 (MQ=255)
                                                                                                                            acacaaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTa                         <  1:953207/143‑1 (MQ=255)
                                                                                                                              cTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATAcc                       <  1:975754/150‑1 (MQ=255)
                                                                                                                               caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTAcca                      <  1:166744/146‑2 (MQ=255)
                                                                                                                               caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                      <  2:674975/146‑1 (MQ=255)
                                                                                                                               caaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCg                      <  2:813193/146‑1 (MQ=255)
                                                                                                                                aaaaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                     <  2:679568/147‑1 (MQ=255)
                                                                                                                                aTCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCg                      >  2:305898/1‑149 (MQ=255)
                                                                                                                                  aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                     >  2:658169/2‑148 (MQ=255)
                                                                                                                                  aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                     >  1:464207/2‑148 (MQ=255)
                                                                                                                                  aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                     >  1:562561/2‑148 (MQ=255)
                                                                                                                                  aaTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                     >  1:649239/2‑148 (MQ=255)
                                                                                                                                   aTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGt                     >  1:761711/1‑147 (MQ=255)
                                                                                                                                   aTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAg                  <  1:43749/150‑1 (MQ=255)
                                                                                                                                   aTGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAg                  <  1:266261/150‑1 (MQ=255)
                                                                                                                                   aTGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAg                  <  1:667214/150‑1 (MQ=255)
                                                                                                                                    tGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAgc                 <  2:26879/150‑2 (MQ=255)
                                                                                                                                    tGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAgc                 <  2:855228/150‑2 (MQ=255)
                                                                                                                                    tGACGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAg                  <  2:761711/149‑1 (MQ=255)
                                                                                                                                    tGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGt                 >  1:403580/1‑150 (MQ=255)
                                                                                                                                      aCGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAgct                >  2:206074/1‑147 (MQ=255)
                                                                                                                                      aCGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAgct                >  2:75551/1‑147 (MQ=255)
                                                                                                                                       cGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctga              >  1:1031049/1‑150 (MQ=255)
                                                                                                                                       cGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctga              >  2:839585/1‑150 (MQ=255)
                                                                                                                                       cGGATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctga              >  2:986790/1‑150 (MQ=255)
                                                                                                                                       cGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttga              >  1:455146/1‑150 (MQ=255)
                                                                                                                                        ggATAAATTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgat             <  1:477985/150‑1 (MQ=255)
                                                                                                                                        ggACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgat             >  2:679368/1‑150 (MQ=255)
                                                                                                                                         gACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgatt            <  2:578449/150‑1 (MQ=255)
                                                                                                                                          aCAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGttgatt            >  1:307169/1‑149 (MQ=255)
                                                                                                                                           taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga          <  2:317463/149‑1 (MQ=255)
                                                                                                                                           taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga          >  1:7525/2‑150 (MQ=255)
                                                                                                                                           taaaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattga          >  1:208610/2‑150 (MQ=255)
                                                                                                                                             aaTTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTtgattgat         >  2:975469/1‑149 (MQ=255)
                                                                                                                                             aaTTAACTTCTCTGCGTCAGTACACCACTGTCGTCGCTGACACCGGAGATATTGCGGCAATGAAGCTGTATCAGCCTCAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGctgattgac         <  2:994661/149‑2 (MQ=255)
                                                                                                                                               ttGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTgatgat      <  2:396905/150‑1 (MQ=255)
                                                                                                                                               ttGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTgatgat      <  1:75391/150‑1 (MQ=255)
                                                                                                                                                tGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGatgatg     >  2:392195/1‑150 (MQ=255)
                                                                                                                                                  aCCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGc    <  2:447864/149‑1 (MQ=255)
                                                                                                                                                  aCCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCt   <  2:556892/150‑1 (MQ=255)
                                                                                                                                                   ccTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTg  >  1:290390/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCATCAGGGCAGACCGGTTACATCCCCCTAACAAGCTGTTTAAAGAGAAATACTATCATGACGGACAAATTGACCTCCCTTCGTCAGTACACCACCGTAGTGGCCGACACTGGGGACATCGCGGCAATGAAGCTGTATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTG  >  CP009273/8107‑8403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑