Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 8,457 A→T 54.9% T74S (ACC→TCC)  talB → transaldolase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092738,4570AT54.9% 48.4 / 203.3 131T74S (ACC→TCC) talBtransaldolase B
Reads supporting (aligned to +/- strand):  ref base A (28/31);  new base T (32/40);  total (60/71)
Fisher's exact test for biased strand distribution p-value = 8.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

Only 100 of 135 total aligned reads displayed.

ATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGT  >  CP009273/8317‑8606
                                                                                                                                            |                                                                                                                                                     
aTCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAAc                                                                                                                                              >  1:711988/1‑150 (MQ=255)
   aaCCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTgg                                                                                                                                           <  1:699478/150‑1 (MQ=255)
    gcctcAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGc                                                                                                                                          >  2:176556/5‑150 (MQ=255)
     cctcAGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGcg                                                                                                                                         <  1:775033/147‑2 (MQ=255)
      cGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAg                                                                                                                                        >  2:517798/1‑150 (MQ=255)
      cGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAg                                                                                                                                        <  1:979953/150‑1 (MQ=255)
       gCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGt                                                                                                                                       <  1:975469/150‑1 (MQ=255)
        cAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGt                                                                                                                                       >  1:343357/1‑149 (MQ=255)
         aGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGtga                                                                                                                                     <  1:206888/150‑3 (MQ=255)
         aGGATGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGtga                                                                                                                                     <  1:906549/150‑3 (MQ=255)
         aGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTaa                                                                                                                                     <  1:108836/150‑1 (MQ=255)
         aGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTa                                                                                                                                      >  2:140427/1‑149 (MQ=255)
           gATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAat                                                                                                                                   <  2:519727/150‑1 (MQ=255)
            aTGCCACCACTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCTCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTgaaca                                                                                                                                  >  1:185072/1‑148 (MQ=255)
            aTGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAata                                                                                                                                  >  1:454058/1‑150 (MQ=255)
                caccacTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCgg                                                                                                                              >  2:503363/1‑150 (MQ=255)
                caccacTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCgg                                                                                                                              >  2:760050/1‑150 (MQ=255)
                caccacTAACCCTTCCCTGATTCTCGGCGCGGCTCAGATCCCCGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTATCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTgaacaccgg                                                                                                                              >  2:380000/1‑144 (MQ=255)
                 accactaaccCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGt                                                                                                                             <  2:672133/144‑1 (MQ=255)
                 accactaaccCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGt                                                                                                                             <  1:735157/144‑1 (MQ=255)
                 accactaaccCTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGt                                                                                                                             <  2:407644/144‑1 (MQ=255)
                    accaaccCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTg                                                                                                                          >  1:504119/1‑150 (MQ=255)
                      caaccCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTgg                                                                                                                         >  2:858636/1‑149 (MQ=255)
                         cccTTCCCTGATTCTCGGCGCGGCTCAGATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAAt                                                                                                                     <  2:636052/150‑1 (MQ=255)
                                   aTTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACt                                                                                                            <  1:874303/149‑1 (MQ=255)
                                     tCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGt                                                                                                         >  1:409548/1‑150 (MQ=255)
                                      ctcggcgcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTgat                                                                                                         <  1:742006/144‑3 (MQ=255)
                                      ctcggcgcggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTgat                                                                                                         <  1:510686/144‑3 (MQ=255)
                                      cTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGt                                                                                                         <  1:148361/149‑1 (MQ=255)
                                         aaCGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTcc                                                                                                      <  2:893918/149‑1 (MQ=255)
                                          aCGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCgg                                                                                                    <  2:290390/150‑1 (MQ=255)
                                             cggctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGcc                                                                                                 <  2:1002145/145‑1 (MQ=255)
                                             cagcgcagATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGcc                                                                                                 <  2:403580/150‑1 (MQ=255)
                                                ctcagATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTa                                                                                              >  1:753897/3‑150 (MQ=255)
                                                   agATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTAtct                                                                                           >  2:207760/1‑150 (MQ=255)
                                                   agATCCCTGAGTACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTAt                                                                                             <  1:863788/148‑1 (MQ=255)
                                                      ttCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCaa                                                                                        >  1:239907/1‑150 (MQ=255)
                                                           gAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTg                                                                                     >  1:274573/1‑148 (MQ=255)
                                                            agtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAg                                                                                  >  2:213273/3‑150 (MQ=255)
                                                             gtACCGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGt                                                                                 <  2:626158/149‑1 (MQ=255)
                                                             aTACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGt                                                                                 <  2:1022081/150‑1 (MQ=255)
                                                              tACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGtt                                                                                <  2:455146/150‑1 (MQ=255)
                                                                ccGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGa                                                                              >  1:808952/1‑150 (MQ=255)
                                                                ccGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGa                                                                              >  2:869755/1‑150 (MQ=255)
                                                                 cGTAAGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCGCCGAAGTGGac                                                                             <  2:464207/150‑2 (MQ=255)
                                                                   tAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgc                                                                           <  2:264520/150‑1 (MQ=255)
                                                                    aaGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgca                                                                          >  2:490684/1‑149 (MQ=255)
                                                                    aaGCTGATTGACGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgca                                                                          >  2:225920/1‑149 (MQ=255)
                                                                     agctgattgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACgcac                                                                         <  2:765364/147‑3 (MQ=255)
                                                                     aGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATgcgc                                                                         >  2:902829/1‑150 (MQ=255)
                                                                      gTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGcgcg                                                                        >  2:227011/1‑150 (MQ=255)
                                                                        tgatttacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTc                                                                      >  2:388288/1‑150 (MQ=255)
                                                                        tgattgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTc                                                                      >  2:64845/1‑150 (MQ=255)
                                                                        tgattgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTc                                                                      >  1:275577/1‑150 (MQ=255)
                                                                         gattgatGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCt                                                                     >  2:58917/1‑150 (MQ=255)
                                                                          attgatGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCt                                                                     <  1:378573/149‑1 (MQ=255)
                                                                          attgacGACGCTGTCGCCTGGGCTCGTAGCCAGAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCt                                                                     <  1:554141/149‑1 (MQ=255)
                                                                              atgatgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCt                                                                <  2:711988/150‑1 (MQ=255)
                                                                                  tgCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGa                                                            >  2:472840/1‑150 (MQ=255)
                                                                                   gCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGa                                                            >  2:46696/1‑149 (MQ=255)
                                                                                         gCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGa                                                      <  1:679368/149‑1 (MQ=255)
                                                                                           cTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGc                                                   <  2:465880/150‑1 (MQ=255)
                                                                                            tGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCg                                                  >  2:722549/1‑150 (MQ=255)
                                                                                              ggCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTc                                                <  1:392195/150‑1 (MQ=255)
                                                                                                 tcgtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAt                                             >  2:659107/8‑150 (MQ=255)
                                                                                                 gAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAAt                                             >  1:582325/1‑150 (MQ=255)
                                                                                                   gtagccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTAt                                             <  1:921285/143‑1 (MQ=255)
                                                                                                   aaCAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTg                                           <  2:90980/150‑1 (MQ=255)
                                                                                                    tagccagAGCAGCGACCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGCGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGc                                          >  2:725292/5‑150 (MQ=255)
                                                                                                     agccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGc                                          >  2:6778/4‑149 (MQ=255)
                                                                                                      gccagAGCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATcgcg                                         <  1:797390/147‑1 (MQ=255)
                                                                                                         agagCAGCGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAg                                     <  2:953251/150‑1 (MQ=255)
                                                                                                         agagCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAg                                     >  2:224273/1‑150 (MQ=255)
                                                                                                             cagcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGcc                                   >  2:838840/4‑147 (MQ=255)
                                                                                                             cagcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCcaa                                 <  2:323932/147‑1 (MQ=255)
                                                                                                             cagcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCcaa                                 <  1:388288/147‑1 (MQ=255)
                                                                                                             cagcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCcaa                                 <  2:783256/147‑1 (MQ=255)
                                                                                                              agcGATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCcaag                                <  1:1027028/148‑2 (MQ=255)
                                                                                                                 gATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGc                             >  1:567966/1‑150 (MQ=255)
                                                                                                                 gATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCg                              >  2:979056/1‑149 (MQ=255)
                                                                                                                 gATCGCGCGCAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCg                              >  2:338743/1‑149 (MQ=255)
                                                                                                                      cgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGAt                        <  1:140427/150‑1 (MQ=255)
                                                                                                                      cgcgcAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGAt                        >  2:1034719/1‑150 (MQ=255)
                                                                                                                        cgcAGCAGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCatcatc                       >  2:550603/1‑149 (MQ=255)
                                                                                                                         gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa                     <  2:208610/150‑1 (MQ=255)
                                                                                                                         gcagcaGATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaa                     <  1:760050/150‑1 (MQ=255)
                                                                                                                          cagcagATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaaa                    <  2:454058/150‑1 (MQ=255)
                                                                                                                          cagcagATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCaaa                    <  2:274573/150‑1 (MQ=255)
                                                                                                                          cagcagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaag                    <  1:986790/150‑2 (MQ=255)
                                                                                                                          cagcagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCaag                    <  1:405071/150‑2 (MQ=255)
                                                                                                                             cagATCCTCGATGCTTCCGATAAGCTGGCGGTGAACATCGGTCTCGAAATTCTGAAACTGATCCCGGGCCGTATCTCCACCGAAGTGGACGCACGTCTCTCCTATGACACCGAAGCGTCTATCGCGAAAGCCAAGCGCATCATCAAGctg                 <  1:160222/150‑2 (MQ=255)
                                                                                                                              agATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAActct                <  2:68190/150‑1 (MQ=255)
                                                                                                                                aTCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTa               >  2:1028114/1‑149 (MQ=255)
                                                                                                                                     ggACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGa         >  2:296632/1‑150 (MQ=255)
                                                                                                                                      gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt        <  1:1018103/150‑1 (MQ=255)
                                                                                                                                      gACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGAt        <  1:776808/150‑1 (MQ=255)
                                                                                                                                           gaccgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTgg   >  2:537135/1‑150 (MQ=255)
                                                                                                                                            accgacAAAGTGGCAGAAAATATAAGAATGGAAAACCTGAAACTGGAAGCGGGAAGTATCACAACAGAAGTTGAAGAGCGTAAAACCTATGACACCGAAGCGAAAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt  <  1:811433/150‑1 (MQ=255)
                                                                                                                                            accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt  <  1:902829/150‑1 (MQ=255)
                                                                                                                                            accgacAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGt  <  2:280812/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
ATCAACCGCAGGATGCCACAACCAACCCTTCTCTCATTCTTAACGCAGCGCAGATTCCGGAATACCGTAAGTTGATTGATGATGCTGTCGCCTGGGCGAAACAGCAGAGCAACGATCGCGCGCAGCAGATCGTGGACGCGACCGACAAACTGGCAGTAAATATTGGTCTGGAAATCCTGAAACTGGTTCCGGGCCGTATCTCAACTGAAGTTGATGCGCGTCTTTCCTATGACACCGAAGCGTCAATTGCGAAAGCAAAACGCCTGATCAAACTCTACAACGATGCTGGT  >  CP009273/8317‑8606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑