breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR12117903_R1.good.fq1,040,522155,370,422100.0%149.3 bases150 bases97.8%
errorsSRR12117903_R2.good.fq1,040,522155,376,890100.0%149.3 bases150 bases97.1%
total2,081,044310,747,312100.0%149.3 bases150 bases97.5%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0092734,631,46958.71.4100.0%Escherichia coli BW25113, complete genome.
total4,631,469100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000007527
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000102
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.007

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0092730.81852

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input15:41:46 21 Feb 202315:42:28 21 Feb 202342 seconds
Read alignment to reference genome15:42:28 21 Feb 202315:49:02 21 Feb 20236 minutes 34 seconds
Preprocessing alignments for candidate junction identification15:49:02 21 Feb 202315:49:46 21 Feb 202344 seconds
Preliminary analysis of coverage distribution15:49:46 21 Feb 202315:51:25 21 Feb 20231 minute 39 seconds
Identifying junction candidates15:51:25 21 Feb 202315:51:28 21 Feb 20233 seconds
Re-alignment to junction candidates15:51:28 21 Feb 202315:53:06 21 Feb 20231 minute 38 seconds
Resolving best read alignments15:53:06 21 Feb 202315:53:59 21 Feb 202353 seconds
Creating BAM files15:53:59 21 Feb 202315:55:23 21 Feb 20231 minute 24 seconds
Tabulating error counts15:55:23 21 Feb 202315:55:58 21 Feb 202335 seconds
Re-calibrating base error rates15:55:58 21 Feb 202315:55:59 21 Feb 20231 second
Examining read alignment evidence15:55:59 21 Feb 202316:06:26 21 Feb 202310 minutes 27 seconds
Polymorphism statistics16:06:26 21 Feb 202316:06:27 21 Feb 20231 second
Output16:06:27 21 Feb 202316:06:50 21 Feb 202323 seconds
Total 25 minutes 4 seconds